Yazarlar
- Bayraktar, Bülent(33)
- Dertli, Enes(33)
- Dirican, Ebubekir(20)
- Akeren, Zahide(18)
- İspirli, Hümeyra(18)
- Kizil, Hamit Emre(17)
- Semerci, Vahide(14)
- Bayram, Sinan(13)
- Kul, Murat(13)
- Sönmez Sarı, Ebru(13)
- Agar, Guleray(12)
- Akman, Onur(12)
- Kırbaş, Zila Özlem(12)
- Akeren, İhsan(11)
- Koçak, Lokman(11)
- Makas, Samet(11)
- Can, Zehra(10)
- Uzunçakmak, Sevgi Karabulut(10)
- Yayla, İbrahim Erdoğan(10)
- Çelik, Eyüp(10)
- Bayraktar, B.(9)
- Bulbuloglu, Semra(9)
- Kızıl, Hamit Emre(9)
- Tekce, Emre(9)
- Aydemir, Uğur(8)
- Daştan, Buket(8)
- Hintistan, Sevilay(8)
- Kalyoncuo, Servet(8)
- Sönmez Sari, Ebru(8)
- Türkmen, Mutlu(8)
- Apaydın, Emine(7)
- Arslaner, Ayla(7)
- Beydemir, Şükrü(7)
- Dombak, Kübra(7)
- Kara, Yakup(7)
- Mercan, Emin(7)
- Semerci Çakmak, Vahide(7)
- Can, Özgür Devrim(6)
- Esgin, Özge(6)
- Güneş, Hüseyin(6)
Yayınlar
- MakaleAçık Erişim2019Scopus SJRQ2Optimal Material Selection for Total Hip Implant: A Finite Element Case Study
Şensoy, Abdullah Tahir; Çolak, Murat; Kaymaz, Irfan; Findik, Fehim
The selection of most proper materials in engineering design is known as an important stage of the design process. In order to successfully complete this stage, it is necessary to have sufficient knowledge about the structure of materials, density, melting point, thermal expansion coefficient, tensile and yield strength, elongation, modulus of elasticity, hardness and many other properties. There are several selection systems that help the design engineer to choose most suitable material that meet the required properties. In the field of bioengineering, the selection of materials and the development of new materials for the clinical needs are increasingly important. In this study, the cases of optimal implant stabilization were investigated, material alternatives for hip prosthesis were evaluated, and optimal materials were determined. Using computerized tomography data with MIMICS software, virtual surgery was applied the hip bone and the implant was attached to bone. Boundary conditions and material properties have been defined, and finite element model has been created. FEA investigation of the mechanical behavior of the hip implant for various material alternatives determined by the CES software showed that the best material candidate is austenitic, annealed and biodurable stainless steel in terms of the micromotions at the implant–bone cement interface regarding osseointegration. This candidate showed 20.69% less strain value than the most commercially used hip implant material, Ti6Al4V. Therefore, the findings of this study suggest that the use of some specific stainless steel materials for implants may reduce the operation cost and increase the operation success for the total hip arthroplasty.
AtıfScopus36WoS28OpenAlex41
İncele - MakaleYalnız künye2019Scopus SJRQ1Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities
Taylan, Osman; Yilmaz, Mustafa Tahsin; Dertli, Enes
Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain. The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure. The NMR spectroscopy analysis revealed that EPS was a levan type EPS as a β-(2 → 6)-linked fructan. The FTIR analysis further confirmed the presence of the furanoid rings in the EPS structure. The levan S81 showed high level of thermal stability determined by DSC and TGA analysis. The lyophilised levan S81 showed a sheet-like compact morphology and its aqueous solution formed spheroidal lumps with a compact structure detected by SEM and AFM analysis, respectively. Importantly the levan S81 showed a high level of immunomodulatory role, induced the anti-inflammatory cytokine IL-4, and exhibited a strong antioxidant capacity with EC50 value 1.7 mg mL−1 obtained by hydroxyl radical scavenging activity test under in vitro conditions. These findings reveal potential of levan S81 for technological purposes and as a potential natural immunomodulatory and antioxidant.
AtıfScopus132WoS112OpenAlex126
İncele - MakaleYalnız künye2019Scopus SJRQ3Synthesis and Biological Evaluation of Novel Tricyclic Pyrrolidinyl (R)-Alcohols and Amines
Şahin, Engin; Kılıç, Hamdullah; Törnük, Fatih; Dertli, Enes
Three new tricyclic pyrrolidinyl alcohols and three tricyclic pyrrolidinyl amines were synthesized, and the antibacterial activities of these compounds were tested against Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes. All tested pyrrolidinyl alcohols and amines showed antibacterial activity to the tested pathogens, but this activity was dependent on the final concentration of the tested compound, and the structure of these compounds were also important for this antibacterial activity. Of the tested compounds, (2R)-2-(octahydro-4,8-ethanocyclohepta[c] pyrrol2(1H)-yl)-2-phenylethanamine (2c) was the most effective compound among the tested pyrrolidinyl alcohols and amines. This study shows a good example of finding new antimicrobials in order to control pathogenic bacteria.
AtıfScopus1WoS1OpenAlex1İncele - MakaleYalnız künye2019Scopus SJRQ2Expert system application for prioritizing preventive actions for shift work: shift expert
Esen, Hatice; Hatipoğlu, Tuğçen; Cihan, Ahmet; Fiğlali, Nilgün
Introduction. Shift patterns, work hours, work arrangements and worker motivations have increasingly become key factors for job performance. The main objective of this article is to design an expert system that identifies the negative effects of shift work and prioritizes mitigation efforts according to their importance in preventing these negative effects. The proposed expert system will be referred to as the shift expert. Methods. A thorough literature review is conducted to determine the effects of shift work on workers. Our work indicates that shift work is linked to demographic variables, sleepiness and fatigue, health and well-being, and social and domestic conditions. These parameters constitute the sections of a questionnaire designed to focus on 26 important issues related to shift work. The shift expert is then constructed to provide prevention advice at the individual and organizational levels, and it prioritizes this advice using a fuzzy analytic hierarchy process model, which considers comparison matrices provided by users during the prioritization process. An empirical study of 61 workers working on three rotating shifts is performed. After administering the questionnaires, the collected data are analyzed statistically, and then the shift expert produces individual and organizational recommendations for these workers.
AtıfScopus8WoS4OpenAlex6İncele - MakaleAçık Erişim2019Scopus SJRQ3Effects of varying essential oil mixture concentrations applied underconditions of different temperature stress on cardiac markers and other blood parameters
Bayraktar, Bülent; Tekce, E.
Cardiac troponin (cTnI) and erythrocyte distribution width (RDW) have been used as specific markers for the evaluation of cardiac arrhythmias and myocardial diseases. In this experimental study, we aimed to determine the dose-dependent administration of an Essential OilMixture (EOM) (Eucalyptus globulusLabill, Thymus vulgaris, Cymbopogon nardus, and Syzygiumaromaticum) on the serum erythrocyte indices and myocardial damage. We used 400 male, oneday-old, Ross-308 chicks. Each group ((n=8) (22°C Control (C), C+250 mL/1,000 L, C+500 mL/1,000 L, C+750 mL/1,000 L), 36°C (stress control (SC), SC+250 mL/1,000 L, SC+500 mL/1,000 L, SC+750 mL/1,000 L) was further divided into eight groups with 50 animals. Each group of 50 chicks was further subdivided into five groups with 10 animals in each compartment. The results showed that the WBC, RBC, HGB, HCT, RDWSD, RDW-CV, and Lymphocyte decreased in groups without exposure to temperature stress (22ºC) compared to the 22ºC control group; whereas, CnTnI, MCHC, NEUT, and IG (p<0.05) increased compared to the control group. In groups exposed to temperature stress (36ºC), the WBC, HGB, HCT, RDW-SD, and Lymphocyte decreased compared to the control group, but MCH, MCHC, NEUT, and IG increased compared to the control. However, cTnI, CK, creatinine, RBC, and RDW had no effect on CD, MONO, EO, and BASO (p<0.05). EOM mixture had no effect on hematological and biochemical parameters.
AtıfScopus9WoS9OpenAlex9İncele - MakaleYalnız künye2019Scopus SJRQ1A novel perspective for Lactobacillus reuteri: Nanoencapsulation to obtain functional fish fillets
Ceylan, Z.; Uslu, Emin; İspirli, Hümeyra; Meral, Raciye; Gavgalı, Mehmet; 'Yilmaz, Mustafa Tahsin; Dertli, Enes
Enrichment of food products with beneficial Lactic Acid Bacteria (LAB) is an important methodology used to develop functional food products. Lactobacillus reuteri E81 was successfully nanoencapsulated into poly (vinyl alcohol)-based nanofibers for its application to the surface of fish fillets. The Scanning Electron Microscopy analysis confirmed the nanoencapsulation of L. reuteri E81 in electrospun nanofibers (LRNF) that had an average diameter of 381.83 ± 130.69 nm. LAB growth in rainbow trout fillets and fatty fish (mackerel) and were found to be 2.92 and in the range of 3.27 and 2.66 log CFU/g, respectively. Importantly, the antioxidative characteristics of fish fillets significantly increased following their coating with LRNF. The results revealed a significant increase in the inhibition of free radicals in the samples treated with nanoencapsulated L. reuteri as compared to control group fish fillet samples. DPPH activity significantly increased to 40.24%, 44.96%, 44.97 in mackerel samples treated with nanoencapsulated L. reuteri (MLR) and increased to 19.76%, 20.87%, 21.46% in rainbow samples treated with nanoencapsulated L. reuteri (RLR). MLR samples exhibited the highest ABTS radical-scavenging activity (1631 μmol TEAC/g). FRAP value of MLR samples was found to be 1.70 times higher than the control group samples.
AtıfScopus50WoS37OpenAlex45İncele - MakaleAçık Erişim2019Scopus SJRQ1Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.
Emsen, Bugrahan; Ozdemir, Ozlem; Engin, Tubanur; Togar, Basak; Cavusoglu, Seyda; Turkez, Hasan
Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2'-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed glial-neuronal non-cancerous cells from Sprague-Dawley rats were also utilized to measure the effects of treatments on non-cancerous cells. Based on median inhibitory concentration values, the data belonged to non-cancerous cells (2486.71 mg/L) showed distinct towering compared to U87MG (80.93 mg/L) cells. The viability of non-cancerous and U87MG cells exposed to extract is decreased in a dose dependent manner. It was also showed that low concentrations of extract notably increased total antioxidant capacity on non-cancerous cells. In addition, various phenolic compounds in extract were detected through high-perfonnance liquid chromatography. The recent results encourage that extract will be able to have therapeutic potential against glioblastoma.
AtıfScopus23WoS20OpenAlex21İncele - MakaleAçık Erişim2018Scopus SJRQ4Yumurtlamanın Son Dönemindeki Yumurtacı Tavukların Rasyonlarına Bor (Ortoborik Asit) İlavesinin Yumurta Kabuk Kalitesi ve Tibia Biyomekaniği Parametreleri ile Serum, Kabuk ve Tibia Mineral Konsantrasyonları Üzerine Etkisi
Kaya, Hacer; Macit, Muhlis
Yumurtacı tavuk rasyonlarına farklı seviyelerde Bor (B) ilavesinin yumurta kabuk kalitesi ve tibia biyomekaniği parametreleri ile serum, kabuk ve tibia mineral konsantrasyonları üzerine etkilerini belirlemek amacıyla yürütülen çalışmada, 62 haftalık Lohman yumurtacı ticari hibrit 0, 50, 75 ve 150 mg/kg seviyelerde B içeren dört farklı rasyonla 12 hafta süreyle yemlenmişlerdir. Rasyona ilave edilen B, yumurta ağırlığı ve kırılma mukavemetini artırıp (P<0.05 ve P<0.06), hasarlı yumurta oranını azaltmış (P<0.01), şekil indeksi ve kabuk kalınlığını etkilememiştir. Rasyona katılan B’nin yumurta kabuğu minerallerinden B, P, Ca (P<0.01) ve Pb seviyesini artırdığı (P<0.05); Fe miktarını düşürdüğü (P<0.01); Zn, Mg, Mn, Na ve S düzeylerini ise etkilemediği (P>0.05) belirlenmiştir. Serum minerallerinden B muameleden etkilenmiş (P<0.01); Cu, Mn, Fe, Na (P<0.01) ve Ca (P<0.05) azalmış; Mg, Zn ve P ise değişmemiştir. Rasyona ilave edilen B’nin tibia kemiği biyomekanik özelliklerinin tamamına etkisi önemsiz (P>0.05) olurken, tibia kemik B mineral konsantrasyonunda artış (P<0.01); Ca, Zn, Mg, P ve Mn mineral konsantrasyonlarında azalmaya yol açtığı (P<0.01) tespit edilmiştir. Sonuç olarak, kanatlılarda rasyona B ilavesinin mineral dengesini pozitif yönde etkilediği düşünülerek yumurta kabuk kırılma mukavemetini iyileştirip hasarlı yumurta oranını azaltmak amacıyla yumurtlamanın son dönemindeki yumurtacı tavukların rasyonlarına 50 mg/kg B ilave edilmesinin yararlı olacağı kanaatine varılmıştır.
AtıfOpenAlex7TR Dizin2
İncele - MakaleYalnız künye2018Some Physical and Chemical Properties, Antioxidant Activities and Phenolic Profiles of Different Marmalades Traditionally Produced in Artvin, Turkey
Topdas, Elif Feyza; Şengül, Memnune; Serencam, Hüseyın; Dogan, Hanife
In this study, some physiochemical properties (color, pH, total and soluble solid, titratable acidity, glucose, fructose and total sugar content, water activity, hydroxymethyl furfural (5-HMF) as well as antioxidant activity and phenolic composition of traditionally produced rosehip (Rosa canina L.), cornelian cherry (Cornus mas L.), blackthorn (Pyrus elaeagrifolia) and Ahlat pear (Pyrus elaeagrifolia) marmalades were determined. In research, the following data were determined that all marmalade samples' total and water soluble dry matter contents were over 55%; water activity and total sugar contents were over 0.79 and 47 g/100g and HMF contents were found between 10.95 and 1094.11 mg/kg. All samples contained gallic acid, catechin, epicatechin, vanillin, chlorogenic acid, caffeic acid, coumaric acid, p-coumaric acid, hesperidin, routine, ellagic acid and quercetin in different ratios. Phenolic substance values of marmalade samples were between 64.67-205.75 µg GAE/g. The highest DPPH radical scavenging activity belonged to the cornelian cherry marmalade (%3.72±0.09). Because of the high phenolic composition and antioxidant activities of rosehip, blackthorn, cornelian cherry and Ahlat pear marmalades besides their significant health benefits may be used for developing functional foods. High HMF contents in blackthorn and Ahlat pear marmalades could be due to long processing time and boiling process at high temperature during conventional production, and consumers should be aware of this problem.
AtıfOpenAlex24TR Dizin18İncele - MakaleAçık Erişim2018Investigation of the Effect of Some Herbal Extracts Used in Different Ratios on Serum Iron Level in Experimentally Heat Stress Created in Broiler
Bayraktar, Bülent; Tekce, Emre
Objective: Iron is found in the structure of hemoglobin, which carries oxygen to tissues with a critical prescription for life. Hemoglobin is a protein involved in iron binding and transport, which can lead to significant morbidity, leading to impaired suppression of the immune system and resistance to infections, as well as impaired brain and motor function. Material and Methods: Iron level in body; there were studies showing that consumption of iron-containing foods, use of certain hormones and medicines, and stress-related effects have been seen. On the other hand, in psychological and heat stress studies that experimentally applied in rats, it has been reported that levels the level was decreasing. In this study, it was aimed to determine the change in serum iron levels by adding essential oil mixture (EOM) with antioxidant effect to drinking water in 22°C and 36°C heat stress in chickens which are the most sensitive animals to stress. In this study, 400 pcs and 1 day old Ross-308 male broiler chicks were used. There are 8 different groups with 50 animals in each group, it will be divided into 10 pcs in each section and the study was carried out from the 15th day to the 42nd day. The study lasted for 42 days, for 22°C stress-free and 36°C stressed groups with 5 subgroups separated (Control, EOM (essential oil mixture)-250, EOM-500, EOM-750. Results: Serum iron levels in the experimental groups were 88.2, 82.2, 103.4, and 98.4 μg/dL at 22°C, respectively, and 88, 78.2, 84.8, and 85 g/L respectively at 36°C stressed broiler groups. Conclusion: It has been determined as a result of this study, serum ıron levels not affected by the EOM added to drinking water (p>0.05).
AtıfOpenAlex6İncele - Derleme MakaleAçık Erişim2018Mutual Interaction Between Phenolic Compounds and Intestinal Bacteria
Erdogan, Ummugulsum; Talay, Rabia
The intestinal bacterial populations constitute the anaerobic bacteria Clostridium, Bifidobacterium, Peptococcus, Eubacterium, Fusobacterium, Peptostreptococcus, Bacteroides, E. coli, Lactobacillus, Klebsiella, Staphylococcus, Streptococcus and Bacillus species. It is known that the bacteria that make up this population are affecting human health. Therefore, any change in the population causes various diseases (obesity, diabetes, hypertension etc.). Prebiotics are needed to restore this disrupted balance and to protect it from diseases. A diet made from natural foods such as fruit and vegetables plays an important role in the regulation of intestinal microbiota. Many fruit and vegetables rich in phenolic components such as corn, rosehip, trabzon, tomato, carrot, soybean, walnut, grapefruit are important in this sense. The purpose of this compilation is; phenolic compounds, general microbiota and intestinal microbiota and explain the effect of intestinal microbiota on phenolic compounds and phenolic compounds on intestinal microbiota.
AtıfOpenAlex1TR Dizin2İncele - MakaleAçık Erişim2018Scopus SJRQ1Antimicrobial Carvacrol in Solution Blow-Spun Fish-Skin Gelatin Nanofibers
Liu, Fei; Saricaoglu, Furkan Tuerker; Avena-Bustillos, Roberto J.; Bridges, David F.; Takeoka, Gary R.; Wu, Vivian C. H.; Chiou, Bor-Sen; Wood, Delilah F.; McHugh, Tara H.; Zhong, Fang
Carvacrol is a volatile monoterpenic phenol and main component of oregano essential oil that shows nonspecific antimicrobial activity against foodborne pathogenic bacteria. Fish-skin gelatin (FSG) nanofibers encapsulating carvacrol (15%, 20%, 25%, and 30%, w/w FSG) were successfully prepared via solution blow-spinning (SBS) technique using lecithin (2.475% wb) as the surfactant. FSG emulsions with lower carvacrol ratios (5% and 10%) showed higher values in particle size and surface tension as well as lower values in viscosity and modulus, which led to failure of maintaining nanofibers shape. The formed carvacrol-FSG nanofibers showed round and smooth morphologies with average fiber diameters ranging from 103.2 to 138.1 nm as the carvacrol ratio increased from 15% to 30%. Carvacrol was evenly dispersed within the interior of nanofiber matrix. All carvacrol-FSG nanofibers showed inhibitive effects against the growth of Escherichia coli, Salmonella enterica, and Listeria monocytogenes. Moreover, nanofibers with lower carvacrol ratios showed bigger inhibition zones for E. coli and L. monocytogenes (20 mm compared with 12.5 mm for lowest to highest carvacrol ratios, respectively). Nanofibers stored at 20 degrees C (51% RH) showed better retention (40% to 60%) for carvacrol during the first 4 weeks of storage, while nanofibers stored at 2 degrees C (70% RH) showed better retention (10% to 30%) at the end of storage.
AtıfWoS28OpenAlex31İncele - MakaleAçık Erişim2018Scopus SJRQ3Effects of two lichen acids isolated from Pseudevernia furfuracea (L.) Zopf in cultured human lymphocytes
Emsen, Bugrahan; Togar, Basak; Turkez, Hasan; Aslan, Ali
The present study aims at assessing the efficacies of olivetoric acid (OA) and physodic acid (PA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) in human lymphocytes (HLs) in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to establish cytotoxicity in HLs. Besides, oxidative stress and genotoxicity were monitored by estimating the changes of total oxidative stress (TOS) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, respectively, in HLs. At the same time, OA- and PA-induced total antioxidant capacity (TAC) levels in HLs were determined. Although especially low concentrations of OA (IC50 = 109.94 mg/L) and PA (IC50 = 665.49 mg/L) did not show cytotoxic effect at high levels in HLs, it was revealed that cytotoxicity was significantly (p < 0.05) associated with oxidative stress and genotoxicity via correlation analysis. While TOS level in HLs did not statistically (p > 0.05) increase in the presence of all treatments (0.5-100 mg/L) of PA, TAC level was increased by PA applications in certain concentrations (0.5-10 mg/L). Overall, the obtained data indicate that OA and especially PA as lichen compounds that do not cause oxidative stress can be a new resource of therapeutics as recognized in the present study with their high antioxidant features.
AtıfWoS20OpenAlex24İncele - MakaleAçık Erişim2018Scopus SJRQ3Qualitative and quantitative structure-activity relationships studies of quercetin derivatives as chemotherapeutic activity
Rouane, Abderrahmane; Tchouar, Noureddine; Kerassa, Aicha; Cinar, Mehmet; Belaidi, Salah
Qualitative and Quantitative structure activity relationship (SAR/QSAR) analysis was applied to eighteen Quercetin derivatives using a combination of various physicochemical, steric, electronic, and structural molecular descriptors. A multiple linear regression (MLR) procedure was used to model the relationships between molecular descriptors and the chemotherapeutic activity of the Quercetin derivatives. The stepwise regression method was used to derive the most significant models as a calibration model for predicting the inhibitory activity of this class of molecules. The best QSAR models were further validated by a leave one out technique as well as by the calculation of statistical parameters for the established theoretical models. High agreement between experimental and predicted inhibitory values, obtained in the validation procedure, indicated the good quality of the derived QSAR models.
AtıfScopus3OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ1Glucan type exopolysaccharide (EPS) shows prebiotic effect and reduces syneresis in chocolate pudding
İspirli, Hümeyra; Demirbaş, Fatmanur; Dertli, Enes
Exopolysaccharides (EPS) of lactic acid bacteria are important biopolymers that can improve the physicochemical properties of food products and act as prebiotics. In this study the physicochemical role and the prebiotic effects of a glucan type EPS with (α1-3) and (α1-6) linkages were assessed in chocolate pudding containing Lactobacillus rhamnosus GG as a probiotic strain. The functions of EPS were determined by developing three different formulations: control, probiotic (Lactobacillus GG) and symbiotic pudding (Lactobacillus GG + EPS) samples. The pH and acidity of the symbiotic pudding sample were higher than the probiotic and the control samples during the 28-day of storage period. Similarly, an important level of increment in Lactobacillus GG levels in symbiotic sample was observed compared to the probiotic sample suggesting the prebiotic role of the α-glucan. Importantly, the syneresis in symbiotic pudding sample reduced significantly compared to other pudding samples which is related with the physicochemical role of glucan type EPS. This study reveals the prebiotic and physicochemical roles of α-glucan type EPS in a chocolate pudding model.
AtıfScopus33WoS28OpenAlex35İncele - MakaleAçık Erişim2018Scopus SJRQ2Isolation and characterisation of lactic acid bacteria from traditional koumiss and kurut
Ispirli, Hümeyra; Dertli, Enes
Lactic acid bacteria (LAB) were isolated from traditional koumiss and kurut samples and identification of these isolates revealed the presence of Lactobacillus fermentum, Enterococcus faecalis, Enterococcus durans, and Lactobacillus helveticus species. L. fermentum and L. helveticus strains were further tested for different characteristics. All tested strains produced heteropolymeric exopolysaccharides (EPS) with the presence of glucose and galactose as sugar monomers whereas three strains produced fructan type EPS as homopolymeric EPS. Important levels of antibacterial and antifungal activities were observed in these strains. All tested strains were sensitive to the antibiotics tested except streptomycin. Half of the tested strains showed important levels of survival ability under mimicking gastrointestinal conditions.
AtıfScopus20WoS14OpenAlex22İncele - MakaleAçık Erişim2018Scopus SJRQ2Synthesis of Enantiomerically Enriched Drug Precursors by Lactobacillus paracasei BD87E6 as a Biocatalyst
Öksüz, Selda; Şahin, Engin; Dertli, Enes
Global sales of single enantiomeric drug products are growing at an alarming rate every year. A total of 7 bacterial strains were screened for their ability to reduce acetophenones to its corresponding alcohol. Among these strains Lactobacillus paracasei BD87E6 was found to be the most successful biocatalyst to reduce the ketones to the corresponding alcohols. The reaction conditions were systematically optimized for the reducing agent Lactobacillus paracasei BD87E6, which showed high enantioselectivity and conversion for the bioreduction. The preparative scale asymmetric reduction of 3-methoxyacetophenone (1h) by Lactobacillus paracasei BD87E6 gave (R)-1-(3-methoxyphenyl)ethanol (2h) with 92% yield and 99% enantiomeric excess. Compound 2h could be used for the synthesis of (S)-rivastigmine which has a great potential for the treatment of Alzheimer's disease. This study demonstrates that Lactobacillus paracasei BD87E6 can be used as a biocatalyst to obtain chiral carbinol with excellent yield and selectivity. The whole cell catalyzed the reductions of ketone substrates on the preparative scale, demonstrating that Lactobacillus paracasei BD87E6 would be a valuable biocatalyst for the preparation of chiral aromatic alcohols of pharmaceutical interest.
AtıfScopus16WoS14OpenAlex15İncele - MakaleYalnız künye2018Scopus SJRQ2Determining the Patient-Specific Optimum Osteotomy Line for Severe Mandibular Retrognathia Patients
Şensoy, Abdullah Tahir; Kaymaz, Irfan; Ertaş, Ümit; Kiki, Ali
Purpose: The purpose of this study is to suggest a patient-specific osteotomy line to optimize the distractor position and thus to minimize the disadvantages of conventional mandibular distraction osteogenesis (MDO) protocols. In addition, this study also aims to compare the conventional MDO protocols with the new MDO protocol proposed in this study in terms of both orthodontic outcomes and mechanical effects of osteotomy level on callus stabilization by means of the finite element method. Methods: A preoperative patient-specific 3-dimensional bone model was created and segmented by using computed tomography images of an individual patient. Virtual orthodontic set-up was applied to the segmented model prior to the virtual surgery. In order to compare the proposed osteotomy line with the conventional lines used in clinical applications, virtual surgery simulations were performed and callus tissues were modelled for each scenario. The comparison of the success of each osteotomy line was carried out based on the occlusion of the teeth. Results: The osteotomy line determined using the method proposed in this study has resulted in far less malocclusion than the conventional method. Namely, any angular deviation from the optimum osteotomy line determined in this study might result in deep-bite or open-bite. On the other hand, the finite element analysis results have indicated that this deviation also negatively affects the callus stability. Conclusion: In order to achieve a better MDO treatment in terms of occlusion of the teeth and the callus stability, the location of the osteotomy line and the distractor position can be computationally determined. The results suggest that MDO protocol developed in this study might be used in clinic to achieve a better outcome from the MDO treatment.
AtıfScopus7WoS4OpenAlex8İncele - MakaleYalnız künye2018Scopus SJRQ2The interactions of cephalosporins on polyol pathway enzymes from sheep kidney
Şengül, Bülent; Beydemir, Şükrü
Context: Cephalosporins are derived from the fungus Acremonium. Due to their strong bactericidal ability, these drugs have to a wide usage in medicine. Objective: An investigation of the effects on sheep renal aldose reductase (AR) and sorbitol dehydrogenase (SDH) of cefoperazone, cefazolin, cefuroxime, ceftazidime and ceftriaxone as cephalosporin drugs was carried out in the present study. Methods: AR and SDH were purified from sheep kidney by ion exchange, gel filtration and affinity methods with approximately 219- and 484-fold, respectively. Some kinetic properties of the enzymes were determined such as optimal pH, optimal ionic strength, optimal temperature, stable pH, Km and Vmax. IC50 values of the drugs were found for each enzyme. Results: While the AR was inhibited by all drugs, SDH enzyme was inhibited by only CXM (IC50 8.10 mM). Interestingly, CZO activated SDH enzyme. This result was evaluated as important for the flow of the polyol reactions. Ki values and inhibition types were determined for AR. However, these values could not have determined for SDH, due to insufficient inhibition. Conclusions: From these results, it was concluded that cephalosporins may have an important effect on flow of the polyol metabolism.
AtıfScopus12WoS12OpenAlex13İncele - MakaleAçık Erişim2018Scopus SJRQ3The anti-genotoxic effect of some lichenic acids
Kizil, Hamit Emre; Ceker, Selcuk; Capik, Ozel; Agar, Guleray
Four lichen secondary metabolites; diffractaic acid, lecanoric acid, lobaric acid and vulpinic acid were evaluated for the anti-genotoxic effects on human lymphocytes in vitro using single cell gel electrophoresis (SCGE) or comet assay. Carbon tetrachloride, a known mutagenic agent was used as the positive control in the comet test system. The results revealed that at all concentrations, the tested compounds mediated a significant decrease in total DNA damage (comet assay) as compared with positive controls (p > 0.05). The most effective doses of lichen acids were found to be 100 μgmL-1 of diffractic acid, lobaric acid and vulpinic acid, and 50 μgmL-1 of lecanoric acid. In addition, it was determined that the most effective among the tested compounds was vulpinic acid.
AtıfScopus4WoS2OpenAlex2İncele
