Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ2Physical, chemical and thermal characterization of bacterial nanocellulose produced using sugar beet molasses by Komagataeibacter sp. SB2569
Kara, Adem; Bayram, Sinan
Bacterial nanocellulose (BNC) is a biopolymer synthesized by bacteria utilizing various carbon sources. The biocompatible and biodegradable nature of BNC offers considerable advantages for its application in fields such as medicine, biomedical engineering, cosmetics, food, nanocomposites, and organoelectronics. Nevertheless, the high production costs necessitate the development of more cost-effective and efficient BNC production methodologies. This study aimed to produce BNC utilizing cost-effective sugar beet molasses. To achieve this, bacteria isolated from various vinegar samples were assessed, revealing that the BNC production potential of these isolates ranged from 2.09 to 3.21 g L⁻1. The 16S rDNA gene of the strain with the highest BNC yield was amplified and sequenced, and it was subsequently deposited in the GenBank database as Komagataeibacter sp. SB2569, with the accession number PV231799.1. XRD analysis revealed characteristic diffraction peaks at 14.8°, 16.5°, 22.6°, and 34.5°, confirming the cellulose 1β crystalline structure and high crystallinity of the synthesized BNC. FTIR analysis exhibited characteristic absorption bandsthat, confirming the presence of β-1,4-glycosidic linkages and the cellulose I structure of BNC. TGA analysis showed an initial weight loss of ~ 5% between 0–100 °C, a major degradation of ~ 75% occurring at 220–350 °C with a maximum decomposition rate at 343 °C, and a residual mass of approximately 10–20% remaining above 600 °C. The findings indicate that the Komagataeibacter sp. SB2569 strain holds significant potential for BNC production using low-cost carbon sources.
AtıfScopus1WoS1OpenAlex2
İncele - MakaleYalnız künye2026Scopus SJRQ1Molecularly engineered bacterial biopolymer as multifunctional interfacial regulators for dendrite-free and stable aqueous zinc-ion batteries
Adhami, Sadaf; Bayram, Sinan; Coskun, Sahin; Sonmezoglu, Savas; Yuksel, Recep
Aqueous zinc-ion batteries (ZIBs) are promising candidates for next-generation energy storage owing to their intrinsic safety, low cost, and high theoretical capacity. However, their practical application remains hindered by dendrite formation and parasitic side reactions such as hydrogen evolution reaction (HER), anode corrosion, and passivation. Herein, we introduce a sustainable and scalable strategy based on a bacterial eumelanin biopolymer (S2–20), synthesized via the oxidative metabolism of Pseudomonas aeruginosa with indole derivatives, and directly deposited onto Zn foil via spin coating. The S2–20 biopolymer provides a conformal, ion-permeable interface that simultaneously guides uniform Zn nucleation, enhances electrolyte wettability, and suppresses parasitic side reactions. Symmetric cells demonstrate significantly increased Zn2+ transference number (0.908), and prolonged cycling stability exceeding 1200 h at 1.0 mA cm−2 and 200 h under harsh conditions up to 20 mA cm−2. Full cells with V2O5 cathodes further confirm remarkable specific capacity (374 mAh g−1 at 1.0 A g−1) and capacity retention, maintaining 150.5 mAh g−1 after 2200 cycles at 1.0 A g−1. This work presents the first demonstration of a bacterial eumelanin biopolymer as a multifunctional interfacial stabilizer for Zn metal, offering a truly green, cost-effective, and scalable pathway toward safe and sustainable aqueous ZIBs.
AtıfOpenAlex1İncele - MakaleAçık Erişim2026Scopus SJRQ2Investigation of structural, optical and optoelectronic properties of eumelanin pigment
Aksoy Pehlivanoglu, Seval; Bayram, Sinan
In this work, the structural, morphological and optical properties of the eumelanin film fabricated on an FTO substrate were first investigated. Then, the effects of illumination intensitiy on the optoelectronic properties of the device fabricated on p-Si substrate (Eumelanin/p-Si) were investigated. The deposited film was identified as polycrystalline from the XRD pattern. The crystallite size was found to be approximately 13 nm. Surface morphology analysis of the film was carried out using atomic force microscopy (AFM) measurements. The surface roughness value (Rq) of the film was determined to be 55.7 nm. The film’s transmittance in the visible region is approximately 83%. The average refractive index in the visible region was calculated to be 1.39. The value of the eumelanin film was determined using both the Tauc technique and the Kubelka-Munk function. The direct and indirect optical bandgaps of eumelanin were calculated using both the Tauc method and the Kubelka-Munk function. The calculated direct and indirect optical bandgap values are, respectively, 2.79 eV and 1.96 eV for the Tauc method, and 2.65 eV and 1.79 eV for the Kubelka-Munk method. The electronic parameters of Eumelanin/p-Si heterostructure were derived from its characteristics. The charge transport mechanisms controlling the diode behavior of the eumelanin/p-Si structure were investigated and analyzed using the power law relationship. measurements performed at various illumination intensities indicated that the device exhibited photoconductive behavior. All the results obtained from this study demonstrate the potential of eumelanin/p-Si device to be used in photonic applications.
- MakaleAçık Erişim2026Scopus SJRQ4Determination of Biological Activities of Encapsulated Bee Venom
Keskİn, Şaban; Bayram, Sinan; Keskİn, Merve
Bee venom is an important bee product used directly in apitherapy applications. Once harvested, bee venom crystallizes rapidly upon contact with air. Furthermore, bee venom that is not produced and stored under optimal conditions deteriorates rapidly. For this reason, there is a need for new methods that can extend the shelf life of bee venom. Encapsulation techniques are widely used to extend the shelf life of bioactive components by immobilizing them within a biocompatible biopolymer. In this study, the potential of encapsulating bee venom using ionic gelation with chitosan, a biocompatible polymer, was investigated. It was found that chitosan had a 91% encapsulation efficiency for bee venom. It has been determined that bee venom inhibits the acetylcholinesterase enzyme by 84%±2.1, whilst its total protein content is 64.2%±1.8. The antimicrobial activity of the encapsulated bee venom was evaluated against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The inhibition zones ranged from 11 to 12 mm. The results showed that the encapsulated bee venom retained its biological activity without significant loss.
- MakaleAçık Erişim2026Scopus SJRQ1Deep Eutectic Solvents as Sustainable Media for Efficient Recovery of Phenolics From Black (Lycium ruthenicum Murr.) and Red (Lycium barbarum Lam.) Goji Berries: Optimization and Functional Evaluation
Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Çevik, Berke; Baştürk, Fatma Nur; Özmener, Bahar; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have been frequently preferred over the past decade as alternatives to conventional solvents for the extraction of bioactive compounds from natural products. Meanwhile, black and red goji berries are widely consumed as functional foods due to their many functional properties reported in the literature. The aim of this study is to evaluate the effectiveness of different DESs in the extraction of individual phenolic compounds from goji berry (Lycium barbarum and L. ruthenicum) fruits, to determine the most suitable DES, and to optimize the extraction conditions. The DES that provided the highest yield in the extraction of individual phenolic compounds from L. barbarum was determined to be proline:citric acid (DES6 – a total of approximately 33,545 µg/kg), while for L. ruthenicum it was choline chloride:acetic acid (DES3 – a total of approximately 37,496 µg/kg). Rutin was the predominant phenolic compound in both species. DESs provided a higher phenolic yield compared to ethanol and water. In addition, L. ruthenicum exhibited higher total phenolics, flavonoids, proanthocyanidins, and antioxidant activity compared to L. barbarum. These findings reveal that DESs are a green, efficient, and sustainable alternative for the extraction of phenolic compounds from goji berries.
AtıfScopus1WoS1
İncele - MakaleYalnız künye2025Scopus SJRQ2Optimized ultrasonic-NaDES extraction of anthocyanins, polyphenolics, and organic acids from chokeberry fruit with blueness and antimicrobial evaluation
Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Kırkıncı, Seran; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have been widely used in food processing in recent years, owing to their remarkable advantages. The aim of this study was to evaluate the potential of DESs for the recovery of bioactive components from chokeberry fruit. First, among the 10 different DESs, the DES that extracted the highest total individual phenolic content (TIPC) from chokeberry was determined. Then, ultrasonically assisted extraction conditions (ultrasonic amplitude, time, and temperature) were optimized by response surface methodology to obtain the maximum amount of TIPC using this DES. According to the results obtained, the natural deep eutectic solvent (NaDES) choline chloride: acetic acid (1:2, 30% water content) provided the highest yield for TIPC extraction and the optimum conditions for maximum TIPC extraction were determined as 100% for ultrasonic amplitude, 50 °C for temperature and 60 min for sonication time. The TIPC content of the extracts prepared with this NaDES was determined between 1740.26 and 3703.14 mg/kg. In addition, the individual organic acid and individual anthocyanin contents of the NaDES extracts, as well as their antioxidant and antimicrobial activities, were determined. Chlorogenic acid (1392.351–2361.48 mg/kg) among phenolic compounds, pyruvic acid (9361.35–47398.04 mg/kg) among organic acids and kuromanin chloride (69748.13–134668.24 mg/kg) among anthocyanins were the dominant compounds in NaDES extracts. However, the extracts showed varying levels of antimicrobial activity against Gram (+) bacteria, Gram (−) bacteria, and yeast-like fungi (Candida strains). Finally, the Blue Applicability Grade Index (BAGI) was used to evaluate the practicality/blueness potential of the chromatographic method used to determine the anthocyanin composition of the DES extracts. In conclusion, NaDES (choline chloride:acetic acid) can be an alternative solvent for the recovery of some individual phenolics, organic acids and anthocyanins under different extraction conditions. In addition, the use of NaDES in the recovery of various functional compounds needed in food, cosmetics, pharmaceuticals, etc., is important for the promotion of sustainability and green technology.
AtıfScopus19WoS18OpenAlex19İncele - MakaleAçık Erişim2025Scopus SJRQ1Electrospinning Encapsulation of Chlorophylls Microwave-Extracted from Spinach Waste and Utilization as Coloring Agent in Yogurt
Isik, Beyza Sukran; Bayram, Sinan; Altay, Filiz
Natural colorants have become a developing market because of consumer preferences, and more cost-effective and stable production is necessary. In the present study, a natural green colorant was obtained via microwave-assisted extraction from spinach waste to produce affordable, environmentally-friendly, and innovative colorants. The extracts were encapsulated with zein via uniaxial and coaxial electrospinning techniques to overcome the stability issues associated with their natural green color. The encapsulation efficiencies of uniaxially encapsulated chlorophyll extracted from spinach waste by microwave were 63.50 and 57.18 for chlorophyll a and b, respectively. The efficiencies of chlorophyll a and b by coaxial electrospinning were found 49.53% and 88.58%, respectively. The microwave-assisted extraction and the addition of eumelanin may contribute positively to the antioxidant activity and thermal stability of electrospun samples. The stability test against light revealed that 61% of chlorophyll a could be preserved by uniaxial electrospinning. In addition, when chlorophyll-loaded nanofibers were incorporated into yogurt, a more stable green color was achieved during storage.
AtıfScopus10WoS9OpenAlex11İncele - MakaleAçık Erişim2024Phytochemical Analysis and Determination of Antioxidative, Antimicrobial And In Vitro Cytotoxic Properties of Black Rosehip (Rosa Pimpinellifolia L.) Fruits Growing in The Northeast of Türkiye
Bayram, Sinan; Kizil, Hamit Emre; Kıcık, Büşra
The aim of this study was to determine the phenolic content of Rosa pimpinellifolia (L.) fruit extract and antibacterial, antioxidant activity and show the cytotoxic effects of different concentrations of Rosa pimpinellifolia L. fruit extract obtained by ultrasonic assisted method on MCF-7 cell line by WST-8 assay. Antibacterial effects analyzed by disk diffusion method and determined that there was no antibacterial effect in 500 μg/mL Rosa pimpinellifolia L. fruit extract dose. In addition, the total phenolic content was determined by the Folin-Ciocalteu method and found that total phenolic content is 37.5±0.2 mg GA/g. Antioxidant activity value was determined as 32.06±1.1 mg TE/g by ABTS method and 23.47±1.6 mg TE/g by DPPH method. The highest cytotoxic effect of Rosa pimpinellifolia L. fruit extract was determined in dose of 40 μg/mL on MCF-7 cell.
AtıfOpenAlex1
İncele - MakaleAçık Erişim2024Scopus SJRQ1Ultrasonic assisted extraction of water-soluble vitamins from minor components of bee pollen with deep eutectic solvents (DESs) as green solvent
Çelik, Saffet; Gerçek, Yusuf Can; Kutlu, Naciye; Bayram, Sinan; Kırkıncı, Seran; Ecem Bayram, Nesrin
In this study, different deep eutectic solvents (DESs) were used for vitamin extraction from bee pollen. The DES combination with the highest total amount of individual vitamins was choline chloride: ethylene glycol (molar ratio 1:2). The response surface method was then used to optimize the extraction conditions. The independent variables used for optimization were ultrasonic power, time, and temperature, while the dependent variables were the total individual amino acids, total individual phenolic compounds, and total individual vitamin contents. Spectrophotometric analyses and antimicrobial activity were also determined in the extracts. In addition, AGREEprep, ComplexGAPI and BAGI tools were used to evaluate the greenness of the method. The results obtained with DES were compared with water, ethanol, and methanol extracts, which were considered control groups. As a result of optimization, the optimum conditions were found to be 75 % for ultrasonic power, 71.5 min for sonication time, and 40 °C for ultrasonic bath temperature. The model's composite desirability value (d) was found to be 0.81. The predicted value and the experimental values were close to each other. Furthermore, the most dominant vitamin in DES extracts was ascorbic acid, the most dominant amino acid was proline, and the most dominant phenolic compound was myricetin. The amounts of these compounds were higher than those obtained from water, ethanol, and methanol extracts. The spectrophotometric analysis results obtained higher yields with DES compared to the control groups. Antimicrobial activity analysis showed that pollen extracts prepared using choline chloride: ethylene glycol had a weak inhibitory effect for Gram-positive and Gram-negative bacteria but not yeast-like fungal strains. In conclusion, DES has been successfully used to extract vitamins and other bioactive compounds from bee pollen. The results obtained showed that DESs can be an alternative to organic solvents for the extraction of vitamins, which are among the minor components of bee pollen. Besides, the potential of the application different DESs for more efficient extraction of vitamins and components with important pharmaceutical properties from bee pollen needs to be evaluated in detail.
AtıfScopus14WoS12OpenAlex14İncele - MakaleAçık Erişim2024Scopus SJRQ1Synthesis of flaxseed gum/melanin-based scaffold: A novel approach for nano-encapsulation of doxorubicin with enhanced anticancer activity
Kumar, Deepak; Pal, Ravi Raj; Das, Neeladri; Roy, Partha; Saraf, Shubhini A.; Bayram, Sinan; Kundu, Patit P.
Doxorubicin is a powerful chemotherapy medicine that is frequently used to treat cancer, but because of its extremely destructive side effects on other healthy cells, its applications have been severely constrained. With the aim of using lower therapeutic doses of doxorubicin while maintaining the same anti-cancerous activity as those of higher doses, the present study designs nano-encapsulation of doxorubicin by acrylamide grafted melanin as core and acrylic acid grafted flax seed gum as shell (DOX@AAM-g-ML/AA-g-FSG-NPs) for studies in-vivo and in-vitro anticancer activity. For biological studies, the cytotoxicity of DOX@AAM-g-ML/AA-g-FSG-NPs was examined on a cancerous human cell line (HCT-15) and it was observed that DOX@AAM-g-ML/AA-g-FSG-NPs exhibited very high toxicity towards HCT-15. In-vivo investigation in colon cancer-inflicted rat model also showed that DOX@AAM-g-ML/AA-g-FSG-NPs showed better anticancer activity against cancerous cells as compared to free doxorubicin. The drug release behavior of DOX@GML-GFS-NPs was studied at several pH and maximum drug release (95 %) was recorded at pH -7.2, and kinetic data of drug release was follows the Higuchi (R2 = 0.9706) kinetic model. Our study is focussed on reducing the side effects of doxorubicin by its nano-encapsulation in acrylamide grafted melanin as core and acrylic acid grafted flax seed gum that will also enhance its efficiency.
AtıfScopus14WoS14OpenAlex15İncele - MakaleAçık Erişim2024Scopus SJRQ2An optimization study for amino acid extraction from bee bread using choline chloride-acetic acid deep eutectic solvent and determination of individual phenolic profile
Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Bayram, Sinan; Ecem Bayram, Nesrin
In this study, the aim was to optimize the extraction conditions (molar ratio, time, temperature) in order to maximize the total individual amino acid (TAA) yield from bee bread, which is a fermented bee product, using choline chloride-acetic acid deep eutectic solvent (DES). In addition to the phenolic profile and amino acid profile investigated by LC-MS/MS, some spectrophotometric (total phenolic content, total flavonoid content, total proanthocyanidin content) and bioactivity (antioxidant/antimicrobial activity) tests were carried out on the extracts. Optimum conditions were determined as 1:2 for molar ratio, 15 min for time and 10 °C for temperature with optimization based on the TAA values of the different extracts (d: 0.99). Molar ratio and temperature were found to have statistically significant effects on TAA values. At optimum conditions, the amount of TAA obtained with DES (29.678 g/kg) was found to be approximately 35.2% and 54.4% higher than that obtained with ethanol and methanol extracts, respectively. All these results indicate that choline chloride-acetic acid DES can be chosen when preparing bee bread extracts, which are evaluated for different areas of use such as food and health due to active ingredient content. Higher active ingredients can be extracted by trying different DES combinations.
AtıfScopus7WoS9OpenAlex11İncele - MakaleAçık Erişim2024Scopus SJRQ1The application of deep eutectic solvents for protein extraction from bee bread (Perga)
Ecem Bayram, Nesrin; Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Baştürk, Fatma Nur; Yıldırım, Nazlıcan
Bee bread is one of the basic nutrients that bees use as a protein source and is formed as a result of the fermentation of bee pollen. In the last few years, this product has become increasingly recognized and consumed among bee products due to its functional properties. However, deep eutectic solvents (DES) have attracted great interest in recent years as promising green alternatives to replace traditional solvents due to their variable viscosity, low vapor pressure, application-specific adjustability, non-flammability and chemical stability. In this study, bee bread was extracted in 12 different DES with different hydrogen bond acceptor (HBA):hydrogen bond donor (HBD) combinations. The DES combination with the highest total protein content was determined to be choline chloride (ChCl)-urea. Then, ultrasonic extraction conditions were optimized for maximum bioactive extraction in extracts prepared with ChCl-urea. The optimum conditions were found as 31.3 %, 25 min, 35 °C for ultrasonic power, extraction time and temperature, respectively. The desirability value (d) of the model was 0.83. The extracts were analyzed for total phenolic content, total protein content, individual polyphenolics, individual amino acids and individual vitamins as well as antimicrobial and antioxidant activity tests. According to the results, total protein, total individual amino acids and total individual phenolics were higher in the extracts obtained with ChCl-urea compared to the extract prepared with conventional solvent (ethanol). In conclusion, all these findings showed that ChCl-urea combination can be an alternative to ethanol depending on the structural characteristics of the target compounds to be extracted from bee bread.
AtıfScopus15WoS12OpenAlex13İncele - MakaleYalnız künye2024Scopus SJRQ3Microwave hydrodistillation of Pelargonium graveolens L’Her leaves: Essential oil profile, phytochemical composition of wastewater, histo-anatomical structure
Boztaş, Kadir; Gercek, Yusuf Can; Türer, Gizem; Canlı, Deniz; Bayram, Sinan; Çelik, Saffet; Kırkıncı, Seran; Basturk, Fatma Nur; Feizollahi, Sepideh; Cevahir Öz, Gül
By-products with remarkable bioactive properties are formed in extraction processes of essential oils from plant materials. In present study, it was aimed to determine the concentration of individual phenolic and organic acid compounds by LC-MS/MS of the by-product (wastewater) collected after the essential oil isolation from Pelargonium graveolens L. and to determine some bioactive properties of this by-product. In addition, the chemical analysis and antibacterial activity of the essential oil extracted from P. graveolens were determined and the histo-anatomical characterization of the glandular trichomes in the leaf sample was performed. As a result, quercetin (95862.22 μg/kg), myricetin (59834.32 μg/kg) and rutin (36522.90 μg/kg) were the phenolic compounds determined in the highest concentrations in the by-product extracted using two different extraction techniques (non-hydrolysis and hydrolysis methods). Moreover, the individual organic acids of the by-product screened in LC-MS/MS was found to be 4487.17 mg/kg in total. When the essential oil component profile of the plant sample was examined, the citronellol (38.78%) compound was found to be dominant, and it was also detected that the essential oil had remarkable bioactive properties. In conclusion, it can be stated that both the essential oil obtained from P. graveolens and the by-product obtained during essential oil isolation can be considered as a source of some bioactive components in many different industrial applications.
AtıfScopus6WoS4OpenAlex6İncele - MakaleAçık Erişim2023Could eumelanin molecule from Streptomyces parvus BSB49 strain be a potential anticancer agent for lung cancer?
Karakas, Ümıt; Bayram, Sinan; Kizil, Hamit Emre; Öztürk, Serdar
Melanins, which are divided into five different subgroups as eumelanin, pheomelanin, allomelanin, neuromelanin and pyomelanin, are a heterogeneous and amor- phous biopolymer family. Although melanin is produced in many different ways today, it is more practical, and more economical to produce melanin using bacterial strains. The eumelanin pigment, which is the subject of this study, was obtained using Streptomyces parvus BSB49 strain, and its antiproliferative effect at various concentrations against small cell (DMS-114) and non-small cell (H-460) lung cancer was determined. According to our results, the synthesized eumelanin molecule was statistically cytotoxic on non-small cell lung cancer cell line (H-460) at concentrations of 3.125 and 1.56 μg/mL after 24 hours of treatment (IC50=8 μg/mL) and only at a concentration of 3.125 μg/mL after 48 hours of treatment (IC50=8.4 μg/mL). In the small cell lung cancer cell line (DMS-114), no statistically cytotoxic concentration was detected (p<0.05).
- Proje2023Derin Ötektik Çözücüler Kullanılarak Arı Ekmeğinden Biyoaktif Bileşenlerin Ultrasonik Destekli Ekstraksiyonunun Optimizasyonu
Kantar, Naciye Kutlu; Bayram, Sinan; Çelik, Saffet; Ecem Bayram, Nesrin; Gerçek, Yusuf Can; Baştürk, Fatma Nur; Yildirim, Nazlican
Arı ekmeği arıların protein kaynağı olarak kullandıkları temel besin maddelerinden birisi olup kovan içeresinde arı poleninin fermantasyonu sonucu oluşmaktadır. Bu ürün fonksiyonel özllikleri sebebiyle son birkaç yıldır arı ürünleri arasında bilinilirliği ve tüketimi artan ürünler arasında yerini almıştır. Bununla birlikte, derin ötektik çözücüler (deep eutectic solvent-DES), değişken viskoziteleri, uygulamaya özel ayarlanabilirlikleri, alev almazlıkları, düşük buhar basınçları ve kimyasal kararlılıkları nedeniyle geleneksel çözücülerin yerini alacak umut verici yeşil alternatifler olarak son yıllarda büyük ilgi görmektedir. Bu nedenle bu çalışmada, arı ekmeği örneği farklı hidrojen bağ alıcısı (HBA):hidrojen bağ donörü (HBD) kombinasyonlarına sahip olan 10 farklı DES?de ekstrakte edilmiş ve en yüksek toplam protein içeriğine sahip DES kombinasyonunun kolin klorid (ChCl)-üre olduğu belirlenmiştir. Daha sonra ChCl-üre ile hazırlanan ekstraktlarda maksimum biyoakif madde ekstraksiyonu için ultrasonik ekstraksiyon koşulları optmize edilmiştir. Yanıt yüzey yöntemi ile yapılan optimizasyon sonucunda optimum koşullar; ultrasonik güç için %31.3, ekstraksiyon süresi için 25 dk ve sıcaklık için 35 °C olarak bulunmuştur. Modelin arzu edilebilirlik değeri ise (d) 0.83 olduğu görülmüştür. Elde edilen ekstraktlarda toplam fenolik madde, toplam protein içerik, bireysel fenolik madde, bireysel amino asit içerik ve bireysel vitamin içerik analzilerinin yanı sıra antioksidan ve antimikrobiyal aktivite testleri gerçekleştirlmiştir. Elde edilen sonuçlara göre toplam protein, toplam bireysel amino asit ve toplam bireysel fenolik içerik, ChCl-üre ile elde edilen ekstraktlarda geleneksel çözücü (etanol) ile hazırlanan ekstrakta kıyasla daha yüksek oranda tespit edilmiştir. Sonuç olarak, elde edilen tüm bu bulgular arı ekmeğinden ekstrakte edilmek istenen hedef bileşiklerin yapısal özelliklerine bağlı olarak ChCl-üre kombinasyonunun etanole alternatif olabileceğini göstermiştir.
- Kitap BölümüAçık Erişim2023Enigmatic secondary metabolites: Microbial melanins and their applications
Narang, Urja; Kurian, Noble K; Bayram, Sinan
Melanin is a biomacromolecule found in all kingdoms of life that ranges from dark brown to black pigment. Eumelanin, allomelanin, neuromelanin, and pheomelanin are all types of melanin. They possess a wide range of bioactive properties, including antioxidant, radioprotective, antimicrobial, antiviral, antiinflammatory, antiproliferative, and photoprotective properties. Melanin has anticancer and antimicrobial properties that make it useful in pharmacology and medicine. In addition, it has applications in cosmetics; to mimic the natural process of these compounds in the skin, many manufacturers have embraced the use of melanin in sunscreens. Furthermore, due to the absorption of ultraviolet rays, mixed electronic-ionic conductivity, and hydration state-dependent electrical properties of melanin, it is used in organic electronics such as solar cells, batteries, and biosensors. This review examines the various functions and applications of this biological pigment, as well as its distinct physicochemical, chemical, bioactive, and anticorrosive properties.
AtıfScopus1OpenAlex1İncele - MakaleAçık Erişim2023Scopus SJRQ2Preparation of Eumelanin-Encapsulated Stereocomplex Polylactide Nano/Microparticles for Degradable Biocompatible UV-Shielding Products
Putri, Oceu Dwi; Petchsuk, Atitsa; Buchatip, Suthawan; Supmak, Wilairat; Kaewsaneha, Chariya; Thananukul, Kamonchanok; Nim, Bunthoeun; Bayram, Sinan; Thumanu, Kanjana; Opaprakasit, Pakorn
The role of eumelanin as a natural pigment in protecting human skin from ultraviolet (UV) light has drawn vast interest in the research and industrial community. Encapsulation of the compound by various shell materials has been extensively studied to optimize and prolong its shielding efficiency from UV penetration through the skin. Polylactide (PLA)-based copolymers have been widely used in the encapsulation of various active compounds due to their biocompatibility and biodegradability that facilitate sustained release of the active compounds. In this work, stereocomplex PLA (sc-PLA) derived from mixtures of poly(D-lactide-caprolactone-D-lactide), P(DLA-b-CL-b-DLA), a triblock copolymer with linear poly(L-lactide), and PLLA are employed to encapsulate eumelanin by an oil-in-water emulsion (O/W) technique. The effect of eumelanin distribution in PLA's enantiomers and ultrasonication on the physicochemical properties, encapsulation efficiency, and release behavior of the nano/microparticles were evaluated. The potential application of the resulting particles for sunscreen products was assessed in terms of UV absorbance and in vitro sun protection factor (SPF). The nano/microparticles show a hollow spherical structure, whose size can be controlled by ultrasonication. The distribution of eumelanin and the ultrasonication process play a key role in the growth of sc-PLA and the crystalline structure of the particles. The highest encapsulation efficiency of 46.6% was achieved for sc-PLA2U particles. The high content of eumelanin and the hollow structure with a large surface area lead to improvement in the UV absorbance and sunscreen performance of the particles, as revealed by the increase in the SPF value from 9.7 to 16.5. The materials show high potential for various applications, especially in cosmetic and pharmaceutical fields, as UV-shielding products.
AtıfScopus3WoS3OpenAlex3İncele - MakaleAçık Erişim2023Scopus SJRQ2Determination of toxicity and radioprotective properties of bacterial and fungal eumelanin pigments
Bayram, Sinan; Aygün, Bünyamin; Karadayi, Mehmet; Alaylar, Burak; Güllüce, Medine; Karabulut, Abdulhalik
Purpose: Determination of the protective property of melanin, an organic polymer class consisting of phenolic and/or indolic compounds isolated from bacteria and fungi, against fast neutron radiation. To show that these melanin samples, which also have antioxidant and metal chelating properties, can be used as an active ingredient for a drug to be developed against neutrons used in nuclear research and medicine. Materials and methods: Bacterial and fungal media were prepared, and melanin pigments were produced and isolated. For molecular characterization of pigments, bacterial genomic DNA extraction, 16S rDNA gene amplification processes, and fungal genomic DNA extraction, ITS1, and ITS4 Gene Regions amplification were performed. The DEL assay was implemented to determine the genotoxicity properties of bacterial and fungal melanin pigments. Samples were prepared in a pad measuring 10 ml volume (60 × 15 mm) at a concentration of 0.2–1 microgram in 1% agarose gel for radiation-absorbed dose measurements. Absorption measurements were made using 241Am-Be fast neutron source and Canberra brand NP series BF3 gaseous detector to determine the neutron radiation absorption capacity of all samples. The results obtained to determine the absorption degrees of melanin samples were compared with paraffin and normal concrete, which are widely used in neutron radiation shielding studies. Results: Melanin pigments were obtained using different bacteria and fungi strains. Afterwards, the fast neutron radiation absorption capacity of these purified pigments were determined. Compared to reference samples, these pigments were found to have slightly lower radiation absorbing ability. In addition to these experiments, cytotoxicity tests were carried out using the Yeast DEL assay technique to evaluate the potential for use of these organic pigments in fields such as medicine and pharmacology. According to the results obtained from the tests, it was determined that these melanin samples did not have any toxic effects. Conclusion: It was determined that these melanin samples have the potential to be used as a radioprotective drug active substance to protect the tissues and cells of people exposed to neutron radiation after a nuclear accident or nuclear war. Giving a drug that will be developed by using these active ingredients before or after people are exposed to a radiation environment can provide great benefits.
AtıfScopus12WoS12OpenAlex17İncele - MakaleAçık Erişim2023Scopus SJRQ2Extraction of Functional Compounds from Tarragon (Artemisia dracunculus L.) by Deep Eutectic Solvents at Different Properties
Can Gerçek, Yusuf; Kutlu, Naciye; Çelik, Saffet; Gıdık, Betül; Bayram, Sinan; Bayram, Nesrin Ecem
In this study, it was aimed to examine the capacity of deep eutectic solvents (DESs) with different contents to extract bioactive compounds from tarragon (Artemisia dracunculus L.) plant. For this reason, the total phenolic-flavonoid content, total proanthocyanidin content and antioxidant/antimicrobial activities of the prepared DES extracts were investigated, as well as the individual phenolic and individual amino acid profiles. According to the results, DES10 had the highest efficiency in terms of its capacity to extract individual phenolics (approximately 59 mg/100 g) and individual amino acids (approximately 2500 mg/kg), and also gave a higher yield compared to ethanol (approximately 44 mg/100 g for individual phenolics and about 19810 mg/kg for individual amino acids) and methanol (approximately 58 mg/100 g for individual phenolics and approximately 21430 mg/kg for individual amino acids). However, the total phenolic content, total flavonoid content and antioxidant activity values of DES extracts were determined between 59.09–77.50 mg GAE/100 g, 28.68–45.55 mg GAE/100 g and 42.96–146.86 mg TE/100 g, respectively. Therefore, it can be recommended to use these green solvents, which are known as environmentally friendly, as an alternative to organic solvents in the process of preparing extracts of this important medicinal plant in different areas.
AtıfScopus8WoS6OpenAlex9İncele - MakaleAçık Erişim2023Scopus SJRQ1Exploring the Charge Transport of a Natural Eumelanin for Sustainable Technologies
Paulin, João V.; Bayram, Sinan; Graeff, Carlos F. O.; Bufon, Carlos C. B.
Eumelanin, the main quinone-based biomaterial of the melanin family of compounds, is emerging as a model for medical and sustainable electronic interfaces due to its biocompatibility, biodegradability, and transducing abilities. The study utilizes current-voltage measurements and impedance/dielectric spectroscopy under a controlled hydration atmosphere to investigate the charge transport of eumelanin produced from the S. parvus BSB49 strain. We show no differences in the electrical response in highly hydrated conditions compared to the standard synthetic eumelanin. Hence, our findings provide the groundwork to assess the potential use of this natural eumelanin in sustainable organic (bio)electronic applications.
AtıfScopus13WoS14OpenAlex16İncele