Yazarlar
- Bayraktar, Bülent(33)
- Dertli, Enes(33)
- Dirican, Ebubekir(20)
- Akeren, Zahide(18)
- İspirli, Hümeyra(18)
- Kizil, Hamit Emre(17)
- Semerci, Vahide(14)
- Bayram, Sinan(13)
- Kul, Murat(13)
- Sönmez Sarı, Ebru(13)
- Agar, Guleray(12)
- Akman, Onur(12)
- Kırbaş, Zila Özlem(12)
- Akeren, İhsan(11)
- Koçak, Lokman(11)
- Makas, Samet(11)
- Can, Zehra(10)
- Uzunçakmak, Sevgi Karabulut(10)
- Yayla, İbrahim Erdoğan(10)
- Çelik, Eyüp(10)
- Bayraktar, B.(9)
- Bulbuloglu, Semra(9)
- Kızıl, Hamit Emre(9)
- Tekce, Emre(9)
- Aydemir, Uğur(8)
- Daştan, Buket(8)
- Hintistan, Sevilay(8)
- Kalyoncuo, Servet(8)
- Sönmez Sari, Ebru(8)
- Türkmen, Mutlu(8)
- Apaydın, Emine(7)
- Arslaner, Ayla(7)
- Beydemir, Şükrü(7)
- Dombak, Kübra(7)
- Kara, Yakup(7)
- Mercan, Emin(7)
- Semerci Çakmak, Vahide(7)
- Can, Özgür Devrim(6)
- Esgin, Özge(6)
- Güneş, Hüseyin(6)
Yayınlar
- MakaleAçık Erişim2020Scopus SJRQ3Effect of probiotics supplementation on adipokine profile (Visfatin, adiponectin and chemerin), intestinal (citrulline) and thyroid function in japanese quail subjected to heat stress; [Einfluss einer probiotischen futterergänzung auf das adipokin-profil (Visfatin, adiponectin und chemerin), und die darm- (citrullin) und schilddrüsenfunktion japanischer wachteln unter hitzestress]
Bayraktar, B.; Tekce, E.
In this study, we aimed to determine the effect of probiotic supplementation of Lactobacillus reuteri E81 (LRE; 200, 400, 600 mg/kg) to the diets of Japanese quail (Coturnix coturnix japonica) reared under heat stress (HS) on serum adipokine (visfatin, adiponectin and chemerin), intestinal (citrulline) and thyroid hormone levels. On day 7 of the trial, the animals were assigned to 8 groups [Control temperature (CT): control (C, without LRE supplementation), LRE-200, LRE-400 and LRE-600 and HS: control (HSC), SLRE-200, SLRE-400 and SLRE-600], each composed of 56 animals of equal body weight. Each group was divided into 4 subgroups withg 14 animals per compartment. During the experiments, the temperature applied to the CT-groups C, LRE-200, LRE-400 and LRE-600 was 25°C and to the HS-groups HSC, SLRE-200, SLRE-400 and SLRE-600 was 37°C. At the end of the study (days 21 and 42), a dose-dependent decrease in citrulline, chemerin, adiponectin, and thyroid hormones and a dose-dependent increase in the visfatin hormone level (p < 0.01) due to LRE supplementation were observed in the HS and CT groups compared to its control. In conclusion, the present study showed that dietary supplementation with 600 mg/kg of LRE affected serum chemerin, adiponectin, T3, T4, TSH and visfatin levels in Japanese quail raised under heat stress (p < 0.001).
AtıfScopus6WoS5OpenAlex2
İncele - MakaleYalnız künye2020Scopus SJRQ1Probiotic properties of lactic acid bacteria strains isolated from pastırma
Topçu, Kübra Cinar; Kaya, Mükerrem; Kaban, Güzin
In this study, the probiotic properties of 80 lactic acid bacteria (LAB) strains isolated from pastırma, a dry-cured meat product, were investigated. For this purpose, strains were investigated for their resistance to bile salt and hydrophobicity abilities. In 6 strains selected as a result of these analyses, development at high salt concentrations, auto-aggregation and co-aggregation, resistance to low pH, and adhesion analyses were performed. The growth percentage of all strains decreased with increasing bile salt concentration at both 25 °C and 37 °C. P. pentosaceus K7, K41, K44, K51 and K81 and P. acidilactici K99 gave the highest values in terms of hydrophobicity ability. The highest auto-aggregation and the highest co-aggregation were found in P. pentosaceus K41 (17.62%) and P. pentosaceus K44 strain (26.23%), respectively. These six strains showed tolerance to high acidity even at pH 2. The strains significantly maintained their viability in the simulated gastric and intestinal fluids. Furthermore, the strains had low adhesion capacity in Caco-2 and HT-29 cell lines and generally showed resistance under gastrointestinal system conditions. As a result, P. pentosaceus K44, P. acidilactici K99 and P. pentosaceus K41 could be considered as probiotic strains.
AtıfScopus55WoS48OpenAlex50İncele - MakaleAçık Erişim2020Scopus SJRQ1Development of particle swarm and topology optimization-based modeling for mandibular distractor plates
Şensoy, Abdullah Tahir; Kaymaz, Irfan; Ertaş, Ümit
Mandibular Distraction Osteogenesis (MDO) is a common clinical procedure to correct mandibular retrognathia. However, since there is not a gold standard for determining the screw positions for current MDO operations, deviation of distraction direction and malocclusion increases. This case results in need of additional operations that affect the callus stability. In these cases, relapse risk increases and remodelling period gets longer. On the other hand, large volume of the distractor plates results in more invasive treatment and negatively affects the patients’ comfort. To overcome these problems, this study offers a new method including; virtual surgery simulation, determining the optimum screw configuration using particle swarm optimization loop linked between MATLAB-PYTHON-ANSYS programs and the design of distractor plate geometry with topology optimization. In order to test the proposed method, two different Finite Element (FE) models, CM and OM, were established based on conventional and optimum method, respectively. FEA results of the current study reveals that OM has 33.56% less displacement compared to CM, and the most critical screw in terms of screw loosening for OM has 35.29% less strain value than CM. These outcomes show OM shows superior callus stability in comparison with CM. On the other hand, redesign of the distractor plates using topology optimization according to the best screw positions provides 43.32% reduction in the total implant volume which means reduced cost and a less invasive MDO operation. Therefore, the clinical use of this protocol is expected to increase the success of the operation by shortening the recovery period.
AtıfScopus13WoS11OpenAlex13İncele - MakaleAçık Erişim2020RP-HPLC-UV Analysis of the Phenolic Compounds, Antimicrobial Activity Against Multi-Drug Resistant Bacteria and Antioxidant Activity of Fruit and Seed of Diospyros lotus L.
Akar, Zeynep; Karakurt, Aylin; Okumus, Funda; Cinemre, Sedanur; Duzgun, Azer Ozad; Akar, Bulent; Can, Zehra
The object of the present study was to investigate the antioxidant, antimicrobial activity and phenolic compounds of fruit and seeds of Diospyros lotus L. The fruits consumed by humans as nutrients were harvested from the plants that grow naturally in the province of Trabzon-Akçaabat, Eastern Black Sea Region of Turkey. The antioxidant activities of fruit and seeds of Diospyros lotus were determined by using four methods (% DPPH radical scavenging activity, FRAP antioxidant power determination, CUPRAC reducing antioxidant activity and total phenolic content (TPC) in five different proportion of methanol-water extracts. All extracts of fruit and seeds of D. lotus analyzed through whole antioxidant analysis methods showed significant antioxidant activity. In addition, antimicrobial activity of fruit and seeds extracted with DMSO was determined against seven standard bacteria and three multi-drug resistance clinical strains. Although fruit extracts did not have the antimicrobial activity against bacteria, seeds showed antimicrobial effect to both standard strains (Gram-negative and Gram-positive) and antibiotic resistance clinical isolates (Klebsiella pneumoniae carrying blaKPC, Acinetobacter baumannii, harboring blaOXA-23 gene and resistance to colistin, and multi-drug resistant Pseudomonas aeruginosa). MICs’ value of plant seed extracts for standard strains was 0.75-25 mg/mL and antibiotic resistant clinical bacteria were 12.5 and 25 mg/mL. Additionally, phenolic compounds in methanol extracts of fruit and seeds were also determined in by HPLC using 19 standards. Gallic acid and chrysin phenolic compounds were the major phenolic compounds in fruit and seeds, respectively.
AtıfScopus8OpenAlex9TR Dizin3
İncele - MakaleYalnız künye2020Scopus SJRQ2Study on the radiation attenuation properties of locally available bees-wax as a tissue equivalent bolus material in radiotherapy
Islam, Sazirul; Mahmoud, K.A.; Sayyed, M.I.; Alim, Bünyamin; Rahman, Md.M.; Mollah, A.S.
In radiation therapy field, bolus material is frequently utilized when treating different areas of a patient, like at the ears or nose, and to provide build-up of dose to the skin surface. The bolus material must be cost effective, suitably flexible to conform to the surface of the patient, and most importantly should be tissue equivalent. The main objective of this work was to estimate the photon attenuation properties of locally available bees-wax and to assess the feasibility of using this bees-wax as a bolus material practice for treatment of cancer patient. The mass attenuation coefficient (μ/ρ) values have been calculated by using MCNP5 simulation code over the wide range of photon energy (0.015–10 MeV). Some related parameters such as the effective atomic number and the mean free path were also calculated to understand the photon attenuation ability of the investigated bolus material. The attenuation properties were also computed for water, tissue and commercial bolus materials for comparison. Evaluation of the results showed that the radiation attenuation properties of locally available bees-wax have a good similarity to water/tissue. Referring to the results of this study, it is proposed that locally available bees-wax is a suitable substance to be used as bolus material practice for treat of cancer patient.
AtıfScopus59WoS53OpenAlex63İncele - MakaleAçık Erişim2020Scopus SJRQ3Investigation of the relationship of apelin hormone response with some physiological parameters in Maedi-Visna infected sheep
Bayraktar, B.; Sait, A.; Takma, Ç.; Tekce, E.
In this study, the objective was to assess the serological characteristics of sheep, during lactation and pregnancy periods, and rams belonging to the Red Karaman and White Karaman-Kangal race infected with maedi-vis-na infection and body condition score (BCS) of ≤2, 3- 3.5 and ≥4 (high) and to analyse the relationship between the level of apelin and its secretion. Apelin level in the blood serum samples obtained from the jugular veinof the sheeps was determined using ELISA method. As a result of the analyses, it was determined that the level of apelin was statistically different between the races; between lactating sheep and pregnant sheep; and sheep and rams. There was no difference between the BCS groups (p > 0.05). Race and sex interaction as well as race, sex and BCS triple interaction effects were found significant (p < 0.05). It was observed that the sheep belonging to the White Karaman-Kangal race were 18 times more likely to be infected with the virus. A decrease in apelin level was observed in the sheep with infection, and it was found that the risk of infection was 0.37 times higher in rams than in lactating sheep (p < 0.05).
AtıfScopus5OpenAlex4İncele - MakaleYalnız künye2020Scopus SJRQ3Production of mannose-containing oligosaccharides by glucansucrase E81 and determination of their functional characteristics
İspirli, Hümeyra; Dertli, Enes
Oligosaccharides are one of the functional ingredients to be used in food technology. In this study, by using an active glucansucrase GTFA-ΔN E81 in the acceptor reaction of mannose, mannose-containing oligosaccharides were produced and their functionality was tested. The formation of the oligosaccharides were visualised by TLC analysis and mannose-containing oligosaccharides up to DP 7 were determined by LC-MS analysis. The presence of the (1,6)Glc and (1,3)Glc units within the oligosaccharides were determined by NMR analysis. The in vitro immune-modulatory functions of the mannose-containing oligosaccharides were determined but no induction in IL-4, IL-10, IL-12 and TNF-α cytokine levels were detected. Importantly this oligosaccharide mixture showed prebiotic effect by triggering the growth of tested probiotics and not affecting the growth of pathogen strains. Our findings reveals the potential of the role of glucansucrases for the production of functional oligosaccharides.
AtıfScopus8WoS7OpenAlex8İncele - MakaleYalnız künye2020Scopus SJRQ1Microbiological, physicochemical, textural characteristics and oxidative stability of butter produced from high-pressure homogenisation treated cream at different pressures
Sert, Durmuş; Mercan, Emin
Microbiological, physicochemical, and textural characteristics, and oxidative stability was investigated of butter produced from raw cream treated with high-pressure homogenisation (HPH) at 0 (control), 10, 20, 30, 50, or 70 MPa. HPH treatment of cream increased lightness and decreased yellowness of butter. Firmness of all butter samples was between 97 (control) and 380 N (70 MPa). HPH decreased the spreadability of butter dependent on increasing pressure. A monomodal fat particle size distribution was determined in all butter samples; D[3.2] ranged from 25.25 to 125.5 μm and HPH decreased average particle size compared with the control. The induction time of butter ranged from 27.40 (control) to 39.61 h (70 MPa). Above 50 MPa, Salmonella, and yeast and moulds were completely inactivated whilst coliforms and staphylococci were not detected in butter from HPH treated cream at 70 MPa, indicating that HPH can improve the microbial quality of butter produced from raw cream.
AtıfScopus22WoS17OpenAlex17İncele - MakaleAçık Erişim2020Scopus SJRQ3The analgesic effect of metformin on paclitaxel-induced neuropathic pain model in rats: By considering pathological results
Hacimuftuoglu, Ahmet; Mohammadzadeh, Maryam; Hjoughi, Ali Taghizadehghale; Taspinar, Numan; Togar, Basak; Nalci, Kemal Alp; Okkay, Ufuk; Gundogdu, Betul
Background and Objective: Metformin (MET) has been used as an antidiabetic agent for type II diabetes. At the same time, recent researches have shown that the clinical improvement of MET is useful for nerve damage. In this study, we investigated the analgesic effect of MET in paclitaxel (PAC)-induced neuropathic pain. Materials and Methods: Forty-two adult, female rats, Wistar strain weighing 220 ± 10 g were randomly divided into 5 experimental groups. PAC was intraperitoneally (IP) administered (2.0 mg/kg) for 4 groups every other day (0, 2, 4, and 6 days). By the 30th day, MET (100, 200, and 400 mg/kg) was administered to 4 groups. Before and after treatment, basal pain threshold values were measured with Randall-Selitto analgesiometer test. At the end of experiment, pathological values were measured in selected regions including brain (motor cortex, M1), spinal cord (L4-L5), sciatic nerve, and muscle. Results: According to our results, PAC-induced neuropathic pain reached to highest level at 14th day. Four hundred milligram/kilogram concentration of MET remarkably decreased PAC-induced neuropathic pain. On the other hand, pathologic features have shown that PAC had significant pathological change in the brain and spinal cord while in the peripheral nerves and muscles had not shown any pathological change. Conclusion: The pathological results of the current study for the first time demonstrated that MET beside of its antidiabetic effects reversed neuropathic pain induced by PAC. Consequently, this research can be promising for cancer patients that suffering from neuropathic pain induced by anticancer drugs.
AtıfScopus12WoS12OpenAlex13
İncele - MakaleAçık Erişim2020Synthesis of novel polymers containing Schiff base as potential antimutagenic and antimicrobial agents
Nartop, Dilek; Tokmak, Erkan; Özkan, Elvan Hasanoğlu; Kızıl, Hamit Emre; Öğütcü, Hatice; Ağar, Güleray; Allı, Sema
In this research study, two novel polymers including Schiff base were prepared by condensation reaction of polystyrene-A-NH2 with 5-nitro-2-thiophenecarboxaldehyde/or 5-(2-nitrophenyl)-2-furancarboxaldehyde. The synthesized polymers were evaluated using the elemental analysis, infrared spectroscopy (IR), gel permeation chromatography (GPC), thermal gravimetric analysis (TG/DTA). In addition, the antimicrobial and antimutagenic study of the polymers were reported. The antibacterial and antifungal activity were investigated using the diffusion method against pathogenic bacterial strains. PA-TC and PA-FC polymers exhibited the highest antimicrobial activity against the S. typhi H. and P. vulgaris, respectively. PA-FC polymer revealed the highest antifungal activity against C. albicans. The antimutagenic effects were evaluated using the sister chromatid exchange (SCE) and micronucleus (MN) methods against the sodium azide (NaN3) in human lymphocyte cells. The results demonstrated that the PA-FC polymer have strong antimutagenic properties, especially in the concentration of 20 µg/mL.
AtıfScopus10OpenAlex2İncele - MakaleYalnız künye2020Scopus SJRQ2Effects of processing methods and extraction solvents on the chemical content and bioactive properties of propolis
Ecem Bayram, Nesrin; Gerçek, Yusuf Can; Bayram, Sinan; Toğar, Başak
The aim of this study was to evaluate the bioactive properties of propolis extract prepared using different solvents and different extraction methods. The extracts were analyzed by gas chromatography–mass spectrometry (GC–MS) and the differences between their antibacterial activities were evaluated by disc diffusion method. At the same time, bioactive properties of different concentrations of propolis extracts were investigated on human umbilical vein endothelial cells (HUVEC). The proliferative effects and cytotoxic effects of the extracts were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyzes, respectively. Total antioxidant capacity (TAC) and total oxidative status (TOS) parameters were used in assessing biochemical effects in the HUVEC cell line. The DNA damage was also analyzed by 8-oxo-2-deoxyguanosine (8-OHdG) level as indicators of genotoxicity. As a result of the MTT analysis conducted within the scope of the present study, the extracts tested were sorted as 95% ethanol extract of propolis (PEE95) > ultrasonic ethanol extract of propolis (PUEE) > 50% ethanol extract of propolis (PEE50) > ultrasonic water extract of propolis (PUWE) in terms of the effectiveness of their cell viabilities. It was observed that high concentrations of PEE95 induced LDH release. In addition to this, our findings have shown that PEE50, PUEE and PUWE increased oxidative stress at high concentrations. According to 8-OH-dG analysis, all tested extracts were found to be non-genotoxic. The results obtained from antibacterial activity and minimum inhibition concentration tests showed that PUEE and PEE95 had stronger antibacterial effects than PEE50 and PUWE. All these results indicated that propolis has beneficial effects for human health and therefore it is a valuable product which can be used as a food supplement.
AtıfScopus23WoS22OpenAlex25İncele - MakaleAçık Erişim2020Scopus SJRQ3The impact of dietary tarragon (Artemisia dracunculus) on serum apelin, brain-derived neurotrophic factor, cardiac troponin concentrations and histopathology of liver tissue in laying hens housed at different stocking densities
Bayraktar, Bulent; Tekce, Emre; Kaya, Hacer; Karaalp, Musa; Turunc, Eylul
Due to its association with several other stress factors (poultry house gases, inadequate ventilation, heat, cold and poor hygiene), the high stocking density is a major stress factor that adversely affects the health and performance of poultry and the quality of the poultry products. Therefore, this experimental study was aimed at analysing the impact of different doses of dietary tarragon (Artemisia dracunculus) on the serum apelin, plasma brain-derived neurotrophic factor (p-BDNF), and cardiac troponin I (cTnI) concentrations, and the correlation between these indicators in laying hens housed at different stocking densities. The aim of this study is to investigate the effects of adding tarragon in different ratios to laying hen rations in the 2ndovulation period on the cTnI, apelin, and BDNF hormone concentrations and the liver histopathology. The experiment was carried out over a period of eight weeks, with 192 Lohman Brown commercial hybrids at 50 weeks of age. Eight groups (four replicates each), composed of laying hens of equal body weight, which were housed at stocking densities of 580 cm2/hen and 810 cm2/hen and received 0, 1, 5 and 10 mmol/kg of tarragon (Artemisia dracunculus) in the feed, were established. At the end of the trial, 96 of the housed egg-laying hens (3 birds in each subgroup, a total of 12 birds in each group) were randomly selected and blood samples were taken from the vena subcutanea ulnaris. The samples collected were analysed for the apelin, p-BDNF, and cTnI contents. The analysis results demonstrated that tarragon supplementation had no effect on the serum apelin, p-BDNF and cTnI concentrations (P > 0.05). The Sub-Groups ST1, ST1.2, and ST6 presented with severe hyperaemia of the sinusoidal, portal and acinar blood vessels, whilst the hyperaemia of these blood vessels was moderate in Sub-Group ST12. Apelin, BDNF, and cTnI can act as protective factors against negative consequences of stress (e.g., stocking density or heat stress).
AtıfScopus5WoS7OpenAlex2İncele - MakaleAçık Erişim2020Scopus SJRQ4Investigation of alterations in PIK3CA and OCT-4 gene expression in breast cancer
Dirican, Ebubekir; Kankaya, Burak; Büyükaşık, Süleyman; Alış, Halil; Velidedeoğlu, Mehmet; İlvan, Sennur; İlvan, Ahmet
Breast cancer (BCa) is one of the most prevalent cancer amongst women and PIK3CA mutations have a crucial role in carcinogenesis. On the other hand, Octamer-Binding Transcription Factor 4 (OCT-4) is common biomarkers of cancer stem cells, which provide to the pathological processes of numerous carcinomas. In this study, we evaluated the association of OCT-4 gene expression levels with the PIK3CA mutation in BCa patients. The mRNA expression level of the OCT-4 gene was detected by qPCR in 44 BCa patients and 30 BCa patients control have positive PIK3CA mutations. Exon 9 and exon 20 of PIK3CA mutations were identified by sequencing. OCT-4 gene expression and PIK3CA mutations were also clinically evaluated in BCa patients. The frequency of PIK3CA mutation in 44 new cases were 32%. PIK3CA mutations were significantly associated with HER2-positive (p = 0.0271) status, Ki-67 status (p = 0.0434) and patient age (p = 0.0149). OCT-4 mRNA expression was significantly higher in the BCa tissue in comparison to normal breast tissue (p = 0.0305). A significant correlation was found between expression of OCT-4 and node status (p = 0.0190). OCT-4 mRNA expression was higher in the PIK3CA mutation tissue than the wild type tissue. However, no association was found between the expression of OCT-4 and the PIK3CA mutation or wild type samples (p = 0.7908). Our results provided that OCT-4 gene and PIK3CA mutations may have a role in development of BCa. OCT-4 mRNA expression level and PIK3CA mutations have a potential important for BCa diagnosis or treatment.
AtıfScopus1WoS1OpenAlex1İncele - MakaleAçık Erişim2020Scopus SJRQ4Effects of race, gender, body condition score and pregnancy on serum apelin levels in ewe
Bayraktar, Bülent; Tekce, Emre; Aksakal, Vecihi; Takma, Çiğdem; Bayraktar, Fatma Gülten; Şengül, Bülent
Apelin is an important adipokine hormone that is released from adipose tissue, which is considered as the energy store of the body, which plays a role in many physiological processes in the body, as well as cardiovascular, immune functions and energy, nutrients and fluid metabolism. In this study, it was aimed to determine the effect of lactation, pregnancy and gender on apelin hormone levels in blood serums belonging to different races of ewe. In the present study, the hormone levels of the pregnant, non-pregnant ewe and rams of the Akkaraman Kangal and Morkaraman races were thin, with different body condition scores (<2, 3 to 3.5 and ≥4). Apelin hormone level was determined by ELISA technique in blood serum samples of ewe's Jugular vein. It was determined that there was a difference in body scores between races and the interaction between race and body score was important (P<0.05). Apelin level in ewe in lactation and pregnant ewe did not change according to body condition scores (P>0.05). In terms of apelin, gender and body condition score, race and gender, and body condition score interactions were found to be significant.
AtıfScopus6WoS6OpenAlex4TR Dizin2İncele - MakaleYalnız künye2020Scopus SJRQ2Hand washing in primary school students using “demonstration, puzzle, dance, song”: A nursing project based on multifaceted skills training
Ozcan, Aysegul; Ozdil, Kamuran; Kaya, Senay Sermet; Sezer, Fatma
Background: The effects of an educational strategy, “demonstration, puzzle, dance, song (DPDS),” on the hand washing skills of primary school students was ex-amined. Method: This study used a quasi-experimental, training research format with a pre-and posttest research design and was conducted by a research team from a university department of public health nursing. The study included 671 (90.2%) of 744 students who attended primary schools in Nevsehir, Turkey. Data were collected with a demographic data collection form and a Hand Washing Skills Evaluation List. McNemar, Fried-man, and Wilcoxon tests were used to analyze findings. Results: Immediately after training, all steps of hand washing were performed at higher levels than before training (p < .01), and the difference between hand washing times was significant (p < .001). Conclusion: Hand washing skills and times increased after undergo-ing hand washing training using the DPDS method. [J Contin Educ Nurs. 2020;51(4):158-166.].
AtıfScopus1WoS1OpenAlex6İncele - MakaleAçık Erişim2020Scopus SJRQ2Characterization of pediococcus acidilactici PFC69 and lactococcus lactis PFC77 bacteriocins and their antimicrobial activities in tarhana fermentation
Kaya, Halil İbrahim; Şimşek, Ömer
Tarhana is a traditional cereal product fermented by lactic acid bacteria (LAB) and yeast strains that has gained special interest recently as an infant nutrition. Tarhana contains wheat flour, yogurt, and various vegetables that might create a microbiological toxicological risk, especially for Bacillus cereus and Staphylococcus aureus. In this study, characterization of the metabolites responsible for antibacterial activity of Pediococcus acidilactici PFC69 and Lactococcus lactis PFC77 strains obtained from tarhana was performed, and antibacterial effects were detected against B. cereus ATCC 11778 and S. aureus ATCC 29213 during the fermentation. A total of 12,800 AU/mL antibacterial activity was observed for the supernatants of the PFC69 and PFC77 strains that were found to be stable at high temperature and in low pH conditions and sensitive to proteases, suggesting the antimicrobial metabolite is a bacteriocin. These bacteriocins were further purified and their molecular sizes were determined as 4.5 and 3.5 kDa, respectively. Importantly, inoculation of PFC69 and PFC77 to tarhana dough significantly decreased B. cereus ATCC 11778 and S. aureus ATCC 29213 amounts from the fifth day of fermentation compared to the control dough samples. P. acidilactici PFC69 and L. lactis PFC77 strains were concluded as bioprotective cultures for tarhana and these strains were offered for other cereal-based fermentations.
AtıfScopus29WoS25OpenAlex33İncele - MakaleAçık Erişim2020Scopus SJRQ3Investigating the adipokine and cardiac troponin response ın experimental thyroid dysfunction; [Odgovor adipokina i srčanog troponina na eksperimentalno izazvanu disfunkciju štitne žlijezde brojlera]
Bayraktar, Bülent; Tekce, Emre; Takma, Çiğdem; Bayraktar, Sevil; Kilinç, Ayten A.; Ülker, Ufuk; Kurtdede, Efe
This study was conducted to investigate adipokine [Apelin and brain-derived neurotrophic factor (BDNF)] and cardiac troponin (cTnI) response that emerged after adding an essential oil mixture (EOM) (Eucalytus glabutus labii, Thymus vulgaris, Cymbopogon nardus, and Syzygium aromaticum) at different rates to the drinking water of broilers in which thyroid dysfunction (hypo-and hyperthyroidism) had been experimentally induced. In the present study, 150 1-day-old Ross-308 male broiler chicks were used. They were divided into five groups, each with 30 animals. The groups were designed to include five subgroups: control (C), hypothyroid, hypothyroid + 250 ppm EOM, hyperthyroid, and hyperthyroid + 250 ppm EOM, with six animals in each group. At the end of the experiment, Apelin, p-BDNF, cTnI, T3, T4, and thyroid-stimulating hormone (TSH) levels were investigated in blood serum samples obtained by cervical dislocation from four randomly selected animals from each subgroup, making 100 animals in total. It was found that EOM administration resulted in a dose-dependent increase in p-BDNF and apelin levels, and a decrease in T4 levels in the experimentally induced hypo-and hyper-thyroidism groups, but did not affect T3 and cTnI levels. Conversely, an increase in TSH level was observed in the hypothyroidism groups, whereas a decrease was observed in groups with hyperthyroidism. This study is the first to examine adipokine (Apelin and BDNF) and cTnI response to EOM administration in thyroid dysfunction.
AtıfScopus3WoS3OpenAlex1İncele - MakaleAçık Erişim2020Scopus SJRQ1Antiamnesic effects of tofisopam against scopolamine-induced cognitive impairments in rats
Üçel, Umut İrfan; Can, Özgür Devrim; Demir Özkay, Ümide; Ulupinar, Emel
In this study, we investigated the potential therapeutic effects of tofisopam, a 2,3-benzodiazepine derivative anxiolytic, on cognitive deficits in rats with scopolamine-induced amnesia. Cognitive performance of the rats was investigated by using the Morris water maze and passive avoidance tests. Changes in motor activity were assessed by using the activity cage and Rota-rod tests and then morphological changes in the hippocampus were assessed via immunohistochemical stainings. The results indicated that scopolamine impaired learning and memory parameters in rats. Worsened cognitive performance, neuronal loss, and decreased hippocampal synaptophysin, Ki-67, and glial fibrillary acidic protein density were observed. Tofisopam administration at a dose of 50 mg/kg for seven days improved the impaired cognitive performance, enhanced the attenuated synaptic transmission in the hippocampus, increased proliferation in subgranular zones, and improved the decrease in astrocytes in amnesic rats. These findings point out the anti-amnesic effects of tofisopam with concomitant improvements in the hippocampal synaptogenesis, neurogenesis, and glial plasticity, for the first time. Presented beneficial effects of tofisopam on cognitive dysfunctions may have a notable clinical value considering the fact that one of the most important side effects of 1,4-benzodiazepines, which are classical anxiolytic drugs, is amnesia. However, these preclinical results need to be confirmed with further clinical studies, first.
AtıfScopus23WoS20OpenAlex22İncele - MakaleAçık Erişim2020Scopus SJRQ2Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49
Bayram, Sinan; Dengiz, Cagatay; Gerçek, Yusuf Can; Cetin, Idil; Topcul, Mehmet R.
In this study, the structure of the purified extracellular eumelanin pigment isolated from Streptomyces spp. was elucidated by detailed analysis via two different spectroscopic techniques (FT-IR and NMR). In vitro antiproliferative effects of eumelanin were evaluated on HeLa cell line. These experiments were carried out with the evaluation of the parameters including cell viability, cell index, and mitotic index. With the cell viability and cell index, IC50 concentration of eumelanin was determined as 10 μM. This result showed that the IC50 concentration of eumelanin decreased the values of cell viability, cell index and mitotic index. These changes are statistically significant (p ' 0.01). The ability of the dissolved eumelanin (250 μg mL−1) to scavenge free radicals was determined via DPPH and ABTS and was shown to be about 87.73% and 75.2%, respectively, compared with standard antioxidants. It was observed that dry weights of eumelanin yield among the selected strains ranged from 160 to 240 mg L−1. The strain with the highest production potential was selected for 16S rDNA sequence analysis and, accordingly, the selected strain BSB49 was identified as Streptomyces parvus and the sequence analysis results were deposited in NCBI under accession number MK894155.
AtıfScopus47WoS40OpenAlex48İncele - MakaleAçık Erişim2020Scopus SJRQ2Effect of the addition of essential fatty acid mixture to the drinking water of the heat stress broilers on adipokine (Apelin, BDNF) response, histopathologic findings in liver and intestines, and some blood parameters
Bayraktar, Bülent; Tekce, Emre; Aksakal, Vecihi; Gül, Mehmet; Takma, Çiğdem; Bayraktar, Sevil; Bayraktar, Fatma Gülten; Eser, Gizem
The purpose of this study was to examine the effect of adding an essential fatty acid mixture (EOM; Eucalyptus globulus Labill, thymus vulgaris, Cymbopogon nardus, and Syzygium aromaticum) to the drinking water in the heat stress broilers on the adipokine (Apelin, BDNF) response, the histopathologic changes in liver and intestines, and some biochemical parameters. This study lasted for a total of 42days, including the physical exercise period (7days) and the fattening period (35days). A total of 400 Ross 308 male broilers (1-d-old) were randomly divided into 8 groups (50 animals per group), each of which was exposed to various conditions of temperature (C: 22°C and SC: 36°C) and treatment dose (C, 250, 500 and 750mL/1000 L). Each group was divided into 5 subgroups, each comprising 10 animals. In the stress-free groups, whereas the Apelin level linearly decreased, the BDNF level linearly increased. In histopathology, the liver tissue was found to be normal in all groups whereas the duodenum villi length was found to increase in the group of 750mL/1000 L. No statistically significant difference was found between the stressed groups and the non-stressed groups in terms of VLDL, Glucose, Total bilirubin, ALT, and TG (p >.05). While Apelin level increased in the stressed groups, the BDNF level increased in the group of 250mL/1000 L. In the histopathological examination, a small amount of coagulation necrosis was detected in hepatocyte, a diffuse hydropic degeneration was observed in hepatocytes, and finally a dilatation and hyperaemia were seen in sinusoid in the groups of EOM-500mL/1000 L and EOM-750 m/1000 L compared to the control group. Whereas there was no difference between the group of EOM-250mL/1000 L and the control group in terms of duodenum villa length, there was a significant decrease in other groups (p <.00). In conclusion, this study showed that adding 250mL/1000 L of EOM to the drinking water had a positive effect on the serum levels of Apelin and p-BDNF in the groups exposed to stress (p <.05).HIGHLIGHTS Heat stress results in serious annual economic losses in the poultry industry. Essential oil fatty mix acids have positive effects on poultry health. Apelin and BDNF may be a protective factor against deleterious consequences of chronic exposition to heat stress.
AtıfScopus12WoS9OpenAlex10İncele
