Bayburt Üniversitesi

Dirican, Ebubekir; Uzunçakmak, Sevgi Karabulut; Özcan, Halil

Purpose: This study aimed to screen for cytochrome b (CYB) mitochondrial DNA (mtDNA) mutations and analyze the mRNA expressions of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia. Materials and Methods: In this study, whole blood was obtained from 44 schizophrenic patients and 41 healthy individuals for DNA (patients) and RNA (patients and control) isolation. Samples for CYB mtDNA mutations were amplified by PCR and identified by Sanger DNA sequencing. RT-PCR and 2- increment increment Ct method was used for mRNA expression of PIK3CA, AKT1, and mTOR genes. Results: In schizophrenia patients, m.15326 A > G (43/44), m.15452 C > A (5/44), m.15078 A > G (3/44), m.14872 C > T (3/44) and m.14798 T > C (3/44) was the most common CYB mtDNA mutations. In silico analysis showed that some of the mutations were associated with the harmful, disease-causing or benign character. The mRNA expression of the PIK3CA, AKT1, and mTOR genes in schizophrenia patients was significantly higher than in healthy individuals. There was a significant moderate positive correlation between the PIK3CA and AKT1 genes. In addition, by ROC analysis, PIK3CA, AKT1 and mTOR genes were found to have good diagnostic power in the patient group. ROC analyzes showed that PIK3CA in particular has significant diagnostic value for schizophrenia patients with 80% sensitivity and 63.4% specificity. Conclusion: Both of CYB mtDNA mutations frequency and PIK3CA, AKT1 and mTOR mRNA expression were higher in schizophrenic patients compared to healthy individuals. We believe that studying these mechanisms in

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Dirican, Ebubekir; Çinar, Trfan

Purpose: The aim of this study is to evaluate the impact of gossypin on the expression level of heat shock proteins (HSPs) genes in different cancer cells. Materials and Methods: Cells were grown under standard culture conditions. Cancer cells were treated with different concentrations (5-100 mu g/ml) of gossypin and cisplatin (50 mu M) as positive control. Cell viability and effective dose range (5-100 mu g/ml) of gossypin were determined by MTT at 24, 48 and 72 hours. After RNA isolation and cDNA synthesis, HSP60 and HSP70 gene expression levels were analyzed using RT-PCR. For gene expression analysis, the 2-(Delta Delta ct) method was used. Results: According to the Mil results, 25-50-100 mu g/ml of gossypin doses were found effective on HSP60 and HSP70 gene expression levels in the cancer cell lines. Gossypin affected with dose-dependently the expression of HSP60 and HSP70 in the three cell lines. In the three cell lines, 50 mu g/ml and 100 mu g/ml of gossypin doses significantly reduced the expression of HSP60 and HSP70 compared to control group. Conclusion: Our results strongly supported the anticarcinogenic effect of gossypin at various doses in different cell lines. However, we believe that further in vivo research and human studies are needed. Our findings suggest that gossypin could be suitable candidate agent for further investigation to develop new strategies for the prevention and/or treatment of different cancer types.

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Comakli, Omer; Comakli, Kemal; Ozdemir, Nesrin; Yilmaz, Mehmet

The goal of this work is to determine the optimum set of parameters by using the Taguchi method in vapour compression heat pump systems. The experimental apparatus consist of an air-to-liquid vapor compression heat pump, a water cooled condenser, a forced air evaporator, an electrical air and water heaters, and various measuring elements and other auxiliary equipments. Refrigerants R22, R407C, and five of their binary mixtures which contain about 0%, 25%, 50%, 75%, and 100% mass fractions of R407C were tested. To determine the effect of the chosen parameters on the system and optimum working conditions, an experimental design method suggested by Genichi Taguchi was used. The most effective parameters are found to be the condenser water mass flow rate for the coefficient of performance and to be condenser water inlet temperature for the exergetic efficiency.

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Gül, Volkan; Coban, Furkan; Öztürk, Erdogan

Vermicompost, which is not harmfull to the environment, and is increasing in agricultural production in terms of both gaining to the soil and benefiting the waste. This research was carried out in 2019 in Bayburt ecological conditions in order to determine the impacts of different doses of solid (250, 500 and 750 kg da(-1)) and liquid (0, 250, 500 and 750 ml da(-1)) vermicompost fertilizers on the flowering and maturation period (days), stem diameter (cm), table diameter (cm), plant height (cm), thousand weight (g) seed yield (kg da(-1)), seed internal rate (%) oil ratio (%) and oil yield (kg da(-1)) of sunflower (Helianthus annuus). In the experiment, it was determined that all parameters were statistically significant compared to p<0.01. The highest seed (218.7 kg da(-1)) and oil yield (75.9 kg da(-1)), which are the most important parameters in oil sunflower, were obtained in 750 kg da(-1) solid and 750 ml da(-1) Liquid vermicompost application (K3S3). As a result, the use of solid and liquid vermicompost application in oil sunflower together will be beneficial in obtaining the highest healthy, high quality seed and oil yield.

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Ustabulut, Mete Yusuf; Boylu, Emrah; Basar, Umut

Turkiye's migrant integration policy, which is one of the most migration-receiving countries in the Middle East owing to regional conflicts during the 2000s, is assessed alongside that of other receiving nations in the Migrant Integration Policy Index (MIPEX). These index ranks nations in eight key categories: labor market mobility, family reunion, education, health, political participation, permanent residence, access to nationality, and anti-discrimination. This study examines Turkiye's change in migration policy ratings by comparing the index 2015 and 2020 with six European countries (Germany, Belgium, France, Norway, Finland, Sweden). It is found that there was a significant disparity in index scores between Turkiye and these European countries in the 2015 MIPEX report; however, this difference has narrowed in the 2020 report. Turkiye's migrant integration policy rankings have improved substantially (+17), according to the 2020 report. As a result, Turkiye's policy indicator has increased from slightly unfavourable to halfway favourable . Although this score and increase are not at the desired level, they may indicate comprehensive improvements in Turkiye's integration policies in the following report.

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Öztürk, Ferhat; Isik, Ahmet; Öztürk, Bilge

The purpose of this study is to reveal the views of preservice elementary mathematics teachers on the concept of instructional activity. Of qualitative research patterns, the case study method has been employed in this study. The research group of the study is made up of a total of 12 preservice elementary mathematics teachers studying the fourth grade at the department of elementary mathematics teaching in a state university located in the Eastern Anatolian Region of Turkey during the fall semester of the 2013-2014 academic year. In the study, to get the opinions of preservice teachers on the concept of instructional activity, a written feedback form containing three open-ended questions was applied to the preservice teachers. Then, content analysis was conducted on the data obtained from the written feedback form. The study has revealed that preservice teachers have different opinions on the concept of instructional activity. (C) 2015 The Authors. Published by Elsevier Ltd.

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