Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Does Lrig1 expression have a relationship with ERBB1 and ERBB2 expression in schizophrenia?
Uzunçakmak, Sevgi Karabulut; Özcan, Halil; Aydin, Pelin; Halici, Zekai
Purpose: This study aims to investigate Lrig1 expression and to reveal the possible relationship between Lrig1, ERBB1 and ERBB2 in schizophrenia. Materials and Methods: In this study, peripheral blood samples of 70 schizophrenia patients and 60 healthy controls were used. Real Time PCR was applied for Lrig1, ERBB1 and ERBB2 gene expression analysis. Results: The level of Lrig1 mRNA was lower in the patients when compared to healthy controls. Expression levels of ERBB1 and ERBB2 were decreased in the patients versus in the healthy controls. According to the receiver operating characteristic curve analysis, the three genes had the power to discriminate the patients from the ERBB1 and ERBB2 expressions in the patients. in schizophrenia.
- MakaleAçık Erişim2025Scopus SJRQ3Investigation of cytochrome B mutations, and UCP2 and STC1 gene expressions in patients with bipolar disorder
Uzunçakmak, Sevgi Karabulut; Özcan, Halil; Dirican, Ebubekir
Objective The aim herein was to investigate mitochondrial cytochrome B (MT-CYB) mutations in individuals with bipolar disorder. Stanniocalcin-1 (STC1) and uncoupling protein 2 (UCP2) mRNA expressions and their relationship with clinical data and each other were also investigated. Method The blood samples of 100 individuals were included in this study. Real-time PCR was used to evaluate mRNA expressions of STC1 and UCP2. Genetic alterations were investigated via Sanger DNA sequencing. An in silico analysis was performed to reveal the phenotypic effects of MT-CYB mutations. Results In the MT-CYB gene of the bipolar disorder patients, the most seen mutations were the T194A A>G mutation at position 1532, G deletion at position 15498, and C>A L236I mutation at position 15452. Most of the mutations appeared to be neutral or benign. The UCP2 and STC1 mRNA expression levels were significantly higher in the patients than in the healthy controls (P = 0.0124 and P < 0.0001, respectively). The area under the curve values of the receiver operating characteristic curve analysis for UCP2 and STC1 were 0.6631 (P = 0.0123) and 0.8059 (P < 0.0001), respectively. No significant relationship was observed between the gene expressions and the routine laboratory findings. There was a positive correlation between the UCP2 and STC1 mRNA expressions in the bipolar disorder patients (r = 0.03559, P = 0.0306). Conclusion Expression of UCP2 and STC1 may be important parameters in bipolar disorder. MT-CYB mutations may be related to gene expressions. Comprehensive studies on bipolar disorder will help better understand UCP2 and STC1 gene functions.
- MakaleYalnız künye2024Investigation of The Relationship Between COVID-19 Severity and Serum Adropin Levels
Tor, Ibrahim Hakki; Aydın, Pelin; Uzunçakmak, Sevgi Karabulut; Halici, Hamza; Özçelik, Cihad
Objective: COVID-19 is a multisystemic disease with high mortality and morbidity. It is very important to understand the pathogenesis of the disease and to develop pharmacological treatment methods. The aim of this study was to measure serum adropin levels in COVID-19 patients in the ward and intensive care unit and to investigate whether this value can be a prognostic factor or a pharmacological treatment target. Methods: 116 volunteer participants were included in the study. Participants were grouped as the control group consisting of patients without any disease, patients diagnosed with COVID-19 and hospitalised in the ward, patients diagnosed with COVID-19 and hospitalised in Level 2 intensive care and patients diagnosed with COVID- 19 and hospitalised in Level 3 intensive care. Venous blood was taken from the patients. Serum adropin levels were measured according to the manufacturer's instructions. ROC analysis was also performed. Results: According to serum adropin measurements, serum adropin levels of patients with COVID-19 who were hospitalised in the ward decreased compared to the control group. In Level 2 intensive care and Level 3 intensive care patients, serum adropin levels decreased compared to patients in the ward. According to Roc analysis, 333.1 ng/L adropin had a specificity of 0.8167 (95%, 0.6956-0.9048) and a sensitivity of 0.6522 (95%, 0.4273-0.8362). Conclusion: COVID-19 is a disease with high mortality and morbidity affecting all systems. With this study, we think that serum adropin levels may be a prognostic factor or a new pharmacological treatment target and may shed light on new studies.
- MakaleAçık Erişim2024Scopus SJRQ3Unveiling the significance of Gremlin-1 as a novel biomarker for enhanced severity prediction in COVID-19 patients
Celik, Muhammet; Tekin, Erdal; Koçak, Mehmet Nuri; Uzunçakmak, Sevgi Karabulut; Bayraktar, Mustafa
Gremlin-1 is associated with lung disease and plays a role in the initiation and progression of pulmonary fibrosis. This suggests that Gremlin-1 may be associated with lung involvement in COVID-19 and poor clinical outcomes and warrants further investigation. This prospective, cross-sectional, single-blind study was the first to investigate Gremlin-1 levels in COVID-19 patients and whether Gremlin-1 levels could be used to predict and guide clinical follow-up in outpatients and inpatients. In this context, serum Gremlin-1 levels were measured and compared in 2 groups of patients who were diagnosed as positive for COVID-19 by RT-PCR and were followed up as outpatients (n = 28) or required hospitalization (n = 30). The median Gremlin-1 values were statistically significantly different between outpatients and intensive care unit patients (0.367 ng/mL [IQR = 0.377], 1.858 ng/mL [IQR = 2.245], respectively) (P < .0001). The area under the ROC curve value to determine the discriminative power of Gremlin-1 was found to be 0.772 (95% CI: 0.672-0.871; P < .0001), and the cutoff value of Gremlin-1 to discriminate between outpatients and hospitalized patients was found to be 1.242 ng/mL with 66.7% sensitivity and 67.2% specificity. Serum Gremlin-1 level is an important biomarker that can be used as a clinical decision-making tool for COVID-19 positive patients.
AtıfScopus2WoS2OpenAlex3
İncele - MakaleAçık Erişim2023The effect of boric acid, calcium fructoborate and potassium boron citrate on bone health in rats
Uzunçakmak, Sevgi Karabulut
The aim of this study was to examine the effects of boron varieties on bone health. For this purpose, twenty-four wistar rats were used. Animals were separated into four groups. The first group was the control group and was treated with nothing. The other three groups were given 3mg/kg boric acid (BA), calcium fructoborate (CaFB) and potassium boron citrate (KBCi) orally by gavage for three weeks. TNF-α, IL1-β, total oxidant level (TOS), total antioxidant level (TAS), osteopontin and osteocalcin levels were measured in serum and bone tissues of animals by ELIZA method. In the results of the study, no significant difference was observed in serum and bone tissue TNF-α and IL-1β levels between the control group and the groups that were treated with boron varieties were applied (p>0.05). Similarly, serum and bone TOS levels did not differ significantly between the control and other groups (p>0.05). However, the serum TAS level increased significantly with the application of BA, CaFB and KBCi in both serum and bone tissue compared to the control (p<0.0001). Serum osteopontin and osteocalcin levels were also significantly increased with the application of BA, CaFB and KBCi, especially in bone tissue, compared to the control group (p<0.0001). The effects of BA, CaFB, and KBCi on bone health have been compared with this study, firstly. This study is promising as a preliminary study for more comprehensive studies to be carried out in order to use boron varieties such as BA, CaFB and KBCi more widely in the future in order to improve bone health, prevent bone diseases or cope with increased stress due to various factors
AtıfOpenAlex2TR Dizin2
İncele - MakaleAçık Erişim2023LRIG1 levels in chronic rhinosinusitis with nasal polyps
Uzunçakmak, Sevgi Karabulut; Sahin, Abdulkadir; Özçelik, Aysegul Tavaci; Halici, Zekai
Purpose: Nasal polyps (NPs), usually occurring together with chronic rhinosinusitis (CRS), are benign masses of mucosal origin arising from inflammation. The transmembrane protein known as leucine-rich repeats and immunoglobulin-like domains 1 (Lrig1) is a member of the Lrig family. Lrig1 is frequently expressed in the respiratory tract and epithelial tissues and can inhibit several signaling pathways involved in cell proliferation. The aim of this study was to determine Lrig1 levels in NP tissues of patients with CRS. Material and Methods: This study included 36 patients with CRS and NPs and 15 patients who underwent rhinoplasty as the control group. The Lrig1 levels of all participants were measured by the ELISA method.Results: This study revealed that Lrig1 levels were significantly lower in NP tissues than in tissues of the control group. The mean level of Lrig1 of the NP tissues was 22.2 ng/ml, while the mean level of the control group was 28.5 ng/ml. According to the results of ROC analysis, Lrig1 levels have the power to distinguish polyp tissues from control tissues (AUC=0.794). Lrig1 levels were higher in tissues with scores of 4-8 than in tissues with scores of 16-20 based on the results of computed tomography scoring. According to endoscopic evaluations, Lrig1 levels of tissues with scores of 5-8 or 9-11 were relatively lower than those of tissues with scores of 2-4.Conclusion: Lrig1 levels were found to be decreased in NP tissues. Thus, Lrig1 may be used to confirm the presence of NPs. Lrig1 may also be helpful in NP grading. Increasing the Lrig1 levels in cases of NPs has the potential to become a targetable treatment modality.
AtıfWoS1OpenAlex1TR Dizin1İncele - MakaleAçık Erişim2023Scopus SJRQ2Evaluation Expression of the Caspase-3 and Caspase-9 Apoptotic Genes in Schizophrenia Patients
Dirican, Ebubekir; Özcan, Halil; Uzunçakmak, Sevgi Karabulut; Takım, Uğur
Objective: Apoptosis is programmed cell death that occurs by several pathways. Caspase-3 is induced by active caspase-9 via the intrinsic pathway. The aim of this research was to explore the expression of caspase-3 and caspase-9 in schizophrenia patients and healthy samples. Methods: RNA was isolated from the peripheral blood of 39 schizophrenia patients’ and healthy samples. After cDNA synthesis, real time PCR (RT-PCR) was used to analyse caspase-3 and caspase-9 gene expression. The severity of psychopathological symptoms of schizophrenia was evaluated using the Positive and Negative Symptoms Scale for schizophrenia (PANSS) and Clinical Global Impressions (CGI). Results: The expression of caspase-3 and caspase-9 genes was higher in schizophrenia patients than in healthy samples (p = 0.012, p = 0.002, respectively). The increase in caspase-3 gene expression was significant with being male, smoking and with a duration of less than 6 years (p = 0.047, p = 0.049, p = 0.034, respectively). On the other hand, the increase in caspase-9 gene expression was significant in patients who is smoke, have children, and are under 33 years old (p = 0.040, p = 0.043, p = 0.045, respectively). A significant positive correlation was detected between the caspase-3 and caspase-9 gene expression (r = 0.3218, p = 0.049). Conclusion: Our findings indicate that caspase-3 and caspase-9 gene expression may activate cell death mechanisms by intrinsic apoptotic genes. Furthermore, caspase-3 and caspase-9 may play essential roles in different ways in schizophrenia. Hence there is a need to further study the apoptotic mechanism with expanded patient populations.
AtıfScopus45WoS47OpenAlex42İncele - MakaleAçık Erişim2023Scopus SJRQ1Reproductive Effects of S. boulardii on Sub-Chronic Acetamiprid and Imidacloprid Toxicity in Male Rats
Sevim, Cigdem; Akpinar, Erol; Aksu, Emrah Hicazi; Ömür, Ali Dogan; Yildirim, Serkan; Kara, Mehtap; Bolat, Ismail; Tsatsakis, Aristides; Mesnage, Robin; Golokhvast, Kirill S. S.
The potential health-promoting effects of probiotics against intoxication by pesticides is a topic of increasing commercial interest with limited scientific evidence. In this study, we aimed to investigate the positive effects of probiotic Saccharomyces boulardii on the male reproductive system under low dose neonicotinoid pesticide exposure conditions. We observed that acetamiprid and imidacloprid caused a degeneration and necrosis of the spermatocytes in the tubular wall, a severe edema of the intertubular region and a hyperemia. This was concomittant to increased levels of 8-hydroxy-2 '-deoxyguanosine reflecting oxidative stress, and an increase in caspase 3 expression, reflecting apoptosis. According to our results, Saccharomyces boulardii supplementation mitigates these toxic effects. Further in vivo and clinical studies are needed to clarify the molecular mechanisms of protection. Altogether, our study reinforces the burden of evidence from emerging studies linking the composition of the gut microbiome to the function of the reproductive system.
AtıfScopus20WoS19OpenAlex24İncele - MakaleAçık Erişim2022CYB mtDNA mutations and expression status of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia
Dirican, Ebubekir; Uzunçakmak, Sevgi Karabulut; Özcan, Halil
Purpose: This study aimed to screen for cytochrome b (CYB) mitochondrial DNA (mtDNA) mutations and analyze the mRNA expressions of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia. Materials and Methods: In this study, whole blood was obtained from 44 schizophrenic patients and 41 healthy individuals for DNA (patients) and RNA (patients and control) isolation. Samples for CYB mtDNA mutations were amplified by PCR and identified by Sanger DNA sequencing. RT-PCR and 2- increment increment Ct method was used for mRNA expression of PIK3CA, AKT1, and mTOR genes. Results: In schizophrenia patients, m.15326 A > G (43/44), m.15452 C > A (5/44), m.15078 A > G (3/44), m.14872 C > T (3/44) and m.14798 T > C (3/44) was the most common CYB mtDNA mutations. In silico analysis showed that some of the mutations were associated with the harmful, disease-causing or benign character. The mRNA expression of the PIK3CA, AKT1, and mTOR genes in schizophrenia patients was significantly higher than in healthy individuals. There was a significant moderate positive correlation between the PIK3CA and AKT1 genes. In addition, by ROC analysis, PIK3CA, AKT1 and mTOR genes were found to have good diagnostic power in the patient group. ROC analyzes showed that PIK3CA in particular has significant diagnostic value for schizophrenia patients with 80% sensitivity and 63.4% specificity. Conclusion: Both of CYB mtDNA mutations frequency and PIK3CA, AKT1 and mTOR mRNA expression were higher in schizophrenic patients compared to healthy individuals. We believe that studying these mechanisms in
AtıfWoS1OpenAlex1TR Dizin1İncele - Derleme MakaleAçık Erişim2022Scopus SJRQ3SARS-CoV-2 Infection and Candidate Biomarkers
Uzunçakmak, Sevgi Karabulut
Severe acute respiratory syndrome coronavirus 2 is a virus that can still infect individuals and whose deadly effects continue despite the current vaccines and drugs. Since 2019, many studies on the pathogenesis of the disease have been completed and continue to be done. In addition to the diagnosis and treatment of the disease, many molecules that can be markers of the disease have been investigated. In the early stages of the pandemic, many nonspecific and infection-related laboratory findings and chest computed tomography were used to obtain information about the diagnosis of the disease. The more individual molecules became associated with the disease yet. The purpose of this review is to summarize the impact and role of many molecules associated with coronavirus disease-2019 infection that have been previously used and newly revealed. Numerous studies are summarized in this review. The obtained data show that previously used laboratory findings and new potential biomarkers are not specific to the disease. New potential biomark-ers have been associated with the severity of the disease itself, as can be seen with lung imaging and even with routine laboratory findings. One of the important points that are seen frequently in studies is that the effectiveness of these molecules has been shown not only in coronavirus disease-2019 infection but also in many other diseases. This removes the pathogenesis of the disease from being a unique mechanism created by the Severe acute respiratory syndrome coronavirus 2 and provides a general perspective formed by viral or bacterial infections. However, there are still many molecular changes that need to be investigated. Future studies will continue to update themselves with the mutations of the virus.
AtıfScopus1WoS1İncele - MakaleAçık Erişim2022Scopus SJRQ4HRM method for identification of TP53 exon 5 and 8 mutations in human prostate cancer patients
Özmerdiven, Çağdaş Gökhun; Dirican, Ebubekir; Ayan, Semih; Tatar, Zeynep; Çakır, Sami; Güler, Yavuz; Karadağ, Abdullah; Soydaş, Tuğba; Uzunçakmak, Sevgi Karabulut; Aru, Melek
Background: The purpose of the present study was to perform a high-resolution melting (HRM) analysis to discover mutations in gene exons 5–8 of tumor protein p53 (TP53), as well as the relationships of these mutations to clinical parameters in prostate cancer (PC). Methods: Genomic DNA was extracted from 50 formalin-fixed paraffin-embedded (FFPE) tissues with PC. Mutations in exons 5 and 8 of TP53 were analyzed using the HRM method. Sanger sequencing was used to describe mutations. Results: According to the HRM analysis results, 21 (42%) PC samples had different normalized and shifted melting curves from other samples. Mutations in TP53 exons 5 and8 were observed in 12 (24%) patients by the Sanger method. The detection sensitivity of the HRM method in exon 5 and exon 8 mutations was 66.7% and 50%, respectively. PSA levels of PC patients with TP53 mutation were found to be lower than that of patients with no mutation (p = 0.8270). However, we did not find any correlations between TP53 mutations and clinical parameters (p > 0.05). Conclusions: HRM analysis is a simple, rapid, and efficient mutation-scanning method for known/unknown mutations in TP53 exons 5and8, as well as an attractive method for detection of mutations and their analysis in FFPE tissues. Additional studies with larger patient populations are warranted to confirm the correlation between the TP53 mutations and PC risk.