Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2024Determination of Cytotoxic and Apoptotic Properties of Lobaric Acid, a Secondary Metabolite of Lichen and Investigation of Its Theoretical Potential
Kizil, Hamit Emre; Agar, Guleray; Ekincioğlu, Yavuz
In this study, we aimed to elucidate some of the mechanisms of cell death induced by lobaric acid in A549 (human lung cancer) cells. For this purpose, the effects of cytotoxic concentrations on p53 and caspase-3 gene expressions were investigated. A549 cells were treated with varying concentrations of lobaric acid (12.5, 25, 50, and 100 µg/ml) for 48 hours and then their viability was evaluated and p53 and caspase-3 mRNA expressions were determined at statistically cytotoxic concentrations of 12.5, 50, and 100 µg/ml. According to beta-actin, it was determined that the increase in lobaric acid concentration revealed an upward trend in p53 and caspase-3 mRNA expressions. Furthermore, quantum chemical parameters such as frontier molecular orbitals, band gap energy and ionization potential, electronic affinity, chemical softness, chemical potential, electrophilicity index and chemical hardness were analyzed. Furthermore, molecular docking was performed to identify the binding sites and the binding behavior of lobaric acid to some target proteins (P53, Caspase-3 and Bcl-2).
AtıfOpenAlex3
İncele - MakaleAçık Erişim2020Asimetrik Schiff Bazı Ni(II) Komplekslerinin Antimutajenik Aktivitesinin Değerlendirilmesi
Nartop, Dilek; Özkan, Elvan Hasanoğlu; Kizil, Hamit Emre; Agar, Guleray; Sarı, Nurşen
Bu çalışmada, asimetrik Ni(II) kompleksleri (NiL1, NiL2, NiL3, NiL4) potansiyel antimutajen özelliklerini incelemek için son zamanlarda grubumuzdan biri tarafından rapor edilen yeni iki aşamalı bir yöntem ile hazırlandı. Lenfositlerdeki sodyum azid kaynaklı antimutagenik etki, kardeş kromatid değişimi (SCE) ve mikronükleus (MN) yöntemleriyle belirlendi. Sentezlenen bileşiklerin antimutagenik özelliklere sahip olduğu ve pozitif kontrol olarak kullanılan sodyum azid (NaN3) 'ün neden olduğu mutajeniteyi azalttıkları belirlenmiştir.
AtıfOpenAlex3İncele - MakaleAçık Erişim2019Analysis of genomic stability and DNA damage in plants exposed to cement dust pollution using the RAPD analysis
Ertürk, Filiz Aygün; Agar, Guleray; Sunar, Serap
One of the most important environmental pollutants is cement dust. In this study, RAPD technology was used todefine the potential genotoxic impact of cement dust on plants. For this purpose, the DNA comparison of 12 plant species(Convolvulus sepium, Astragalus christianus, Taraxacum androssovii Medicago varia, Alyssum murale, Artemisiaspisigera, Falcaria vulgaris, Anchusa strigose, Glaucium leiocarpum, Salvia syriaca, Cryciata taurica Tragopogonalbinervis)collected from the areas of 10000 m (Control area) or 0-100 m away from Askale cement factory in Erzurum;was performed. 16 primers were used, and 390 bands were obtained. Important differences were observed in plant RAPDprofiles collected from 0-100 m areas when compared to their respective controls (10000 m away from the factory). Somebands in control plants got lost, and new band formations were observed. The Genomic template stability (GTS) valuedecreased significantly in plants collected from 0-100 m area as compared to the controls (%56.20). Soil samples from 0-100 m and control area were collected and examined for their heavy metal contents. According to the analysis results,Nickel, Cadmium, Zinc, Lead and Copper concentrations were found to be dramatically high in the soil collected from 0-100 m as compared to the control area. These results indicate that the high heavy metal content in the soil causes thechanges in RAPD profiles and GTS in plants. The data also indicated that RAPD and GTS assays are basic, effective andreproducible means of the genotoxicity control of environmental pollution.
AtıfOpenAlex1İncele - MakaleYalnız künye2018Scopus SJRQ2Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine
Hasanoglu Özkan, Elvan; Kizil, Hamit Emre; Sakiyan, Iffet; Nartop, Dilek; Sari, Nursen; Agar, Guleray
The purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.
AtıfWoS3
İncele - MakaleAçık Erişim2018Scopus SJRQ3The anti-genotoxic effect of some lichenic acids
Kizil, Hamit Emre; Ceker, Selcuk; Capik, Ozel; Agar, Guleray
Four lichen secondary metabolites; diffractaic acid, lecanoric acid, lobaric acid and vulpinic acid were evaluated for the anti-genotoxic effects on human lymphocytes in vitro using single cell gel electrophoresis (SCGE) or comet assay. Carbon tetrachloride, a known mutagenic agent was used as the positive control in the comet test system. The results revealed that at all concentrations, the tested compounds mediated a significant decrease in total DNA damage (comet assay) as compared with positive controls (p > 0.05). The most effective doses of lichen acids were found to be 100 μgmL-1 of diffractic acid, lobaric acid and vulpinic acid, and 50 μgmL-1 of lecanoric acid. In addition, it was determined that the most effective among the tested compounds was vulpinic acid.
AtıfScopus4WoS2OpenAlex2
İncele - BildiriAçık Erişim2017Scopus SJRQ1Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells
Kizil, Hamit Emre; Agar, Guleray
- BildiriAçık Erişim2017Scopus SJRQ1Determination of the Antigenotoxic effects of Evernic acid against genotoxic effects of CCL4 on HUVEC lines
Agar, Guleray; Kizil, Hamit Emre; Ceker, Selcuk; Capik, Ozel; Sengul, Bulent
- BildiriAçık Erişim2015Scopus SJRQ1Antiproliferative effects of Evernic acid on A549 and healthy human cells: An in vitro study
Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa
AtıfWoS3OpenAlex4İncele - BildiriAçık Erişim2015Scopus SJRQ1Determination of cytotoxic and proliferative effects of 2-amino-5-nitrobenzonitrile on AGS cancer cell line
Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray
- MakaleAçık Erişim2015Scopus SJRQ2Genotoxic and antigenotoxic potentials of two Usnea species
Ceker, Selcuk; Orhan, Furkan; Kizil, Hamit Emre; Alpsoy, Lokman; Gulluce, Medine; Aslan, Ali; Agar, Guleray
For ages, lichens have long been investigated popularly for biological roles, mainly antitumor, antimicrobial and antioxidant activities. Many positive results were obtained in these previous research. Thus, in this study, we aimed to determine whether extracts of Usnea articulata (UAE) and Usnea filipendula (UFE) possessing a protection against aflatoxin B1(AFB1)-induced genotoxic and oxidative damage. The results of our studies showed that 5 μM concentrations of AFB1increased the frequencies of sister chromatid exchange (SCE) and the level of malondialdehyde (MDA) and decreased the activities of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx). However, when 5, 10 and 20 µg/mL concentrations of UAE and UFE was added to AFB1, the frequencies of SCE and MDA level were decreased and SOD, GSH and GPx level were increased. The Ames (Salmonella typhimurium TA1535, TA1537) and WP2 (Escherichia coli) test systems carried out evinced that UAE and UFE possess any mutagenicity, but have antimutagenic effects. Consequently, the results of this experiment have clearly shown that UAE and UFE have strong antioxidative and antigenotoxic effects that are associated with its antioxidant nature. A detailed study can be performed to determine the antioxidant properties of each compound that will extend the use of lichen extracts in food and pharmacy industries.
AtıfScopus16WoS13OpenAlex14
İncele - MakaleYalnız künye2015Scopus SJRQ3Determination of antigenotoxic effects of four lichen species by using human lymphocytes
Anar, Mustafa; Asian, Ali; Ceker, Selcuk; Kizil, Hamit Emre; Alpsoy, Lokman; Agar, Guleray
In this study, the geno-toxic and antigeno-toxic properties of the total extracts of four lichen species were investigated by using human lymphocytes. We obtained total extracts from R farinacea, X. parietina, U. articulataand U. filipendula lichen species. The results of our studies showed that 5 μM concentrations of AFB1 changed the frequencies of micronucleus (MN) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx) activities. When 5,10 and 20 μg/ml concentrations of total extract were added to AFB1, the frequencies of MN and MDA levels were decreased, and SOD, GSH and GPx levels were increased. Consequently, our findings show that four lichen total extracts have strong antigenotoxic properties against aflatoxin Bi.
AtıfScopus3İncele - BildiriAçık Erişim2014Scopus SJRQ1The determination of antioxidative effect and anticancer potential of vulpinic acid
Anar, Mustafa; Kizil, Hamit Emre; Agar, Guleray
AtıfOpenAlex1İncele - BildiriAçık Erişim2014Scopus SJRQ1Cytotoxic and antiproliferative effects of evernic acid on HeLa cell lines: A candidate anticancer drug
Kizil, Hamit Emre; Agar, Guleray; Anar, Mustafa
AtıfWoS6OpenAlex10İncele - MakaleAçık Erişim2013Scopus SJRQ4Protective Role of Mentha longifolia L. ssp. longifolia against Aflatoxin B
Ceker, Selcuk; Agar, Guleray; Alpsoy, Lokman; Nardemir, Gokce; Kizil, Hamit Emre; Mete, Ebru
The aim of this study was to investigate the effects of essential oil of Mentha longifolia L. ssp. longifolia, used since ancient times for the treatment of many digestive tract diseases, on the genotoxicity and oxidative stress by a single dose of Aflatoxin B1 (5 μM) in the human lymphocyte culture. The total of the 12 identified components accounted for 97.69 % by Gas chromatography (GC). Major components of the oil were cis-piperitone epoxide (26.52 %), piperitenone oxide (26.40 %), pulegone (15.66 %) and piperitenone (13.57 %). According to the results obtained from GC-MS systems, monoterpenes consist of 89.19 % of the total oil approximately while sesquiterpenes were represented in small quantities in the oil. We studied the anti-genotoxic effects of essential oils of M. longifolia (MEO) by using sister chromatid exchange (SCE) and micronucleus (MN) test systems. Also, the anti-oxidative effects of MEO were evaluated by using superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) assay. The SCE frequency and the number of MN were increased when treated with AFB1. Especially, MEO at 1.5 and 2.0 μl reduced SCE frequency and MN number in the human lymphocyte (p <0.05). In addition, AFB1 treatment significantly reduced the activity of SOD and GPx and elevated the level of MDA (p<0.05). MEOs treatment led to significant attenuation of AFB1-induced oxidative stress by normalization of the activities of SOD and GPx and the MDA (p < 0.05). These results suggest that MEOs could provide anti-oxidative and anti-genotoxic protection for the oxidative and genotoxic agents that cause many diseases including cancer and neurodegenerative disease.
AtıfScopus10WoS11OpenAlex11İncele - MakaleYalnız künye2012Scopus SJRQ4Investigation of anti-oxidative and anti-genotoxic effects of Origanum vulgare L. essential oil on human lymphocytes in vitro
Ceker, Selcuk; Agar, Guleray; Nardemir, Gokce; Anar, Mustafa; Kizil, Hamit Emre; Alpsoy, Lokman
Origanum vulgare L. (Lamiaceae) is a flavoring herb widely using around the world. Some preparations and extracts from O. vulgare have been used in Turkish traditional medicine to treat several ailments including digestive disorders and menstrual problems. This study is designed to examine the chemical composition and in vitro antigenotoxic and antioxidant activity of the essential oil of O. vulgare (OVE) from Turkey. The essential oil of O. vulgare was analized by using GC. OVEs that were investigated antagonistic effects against oxidative stress and the genotoxic effects of aflatoksin B1 (AFB1). Anti-genotoxic activity was evaluated by sister chromatid exchanges (SCEs) and micronucleus (MN) tests. In additional to investigating protective role of OVEs against the oxidative stress of AFB1 in vitro, oxidative status was assessed on erythrocytes by measuring following oxidative stress markers like; super oxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA).The total of the 25 identified components are accounted for 96.03 % (GC). Major components of the oil were β-caryophyllene (20.40%), and caryophyllene oxide (34.44%). In the SCE and MN tests, it was observed that OVEs suppressed the mutagenic effects of AFB1. OVEs were observed to modulate the adverse effects of AFB1. The results of this experiment clearly reveal that OVEs have strong anti-oxidative and anti-genotoxic effect. These anti-genotoxic effects of O. vulgare essential oils are probably related to its action on the enzymatic activation system. Anti-oxidative and anti-genotoxic effect may be role of these compounds (β-caryophyllene and caryophyllene oxide).
AtıfScopus14WoS16OpenAlex12İncele