Yazarlar
- Bayraktar, Bülent(33)
- Dertli, Enes(33)
- Dirican, Ebubekir(20)
- Akeren, Zahide(18)
- İspirli, Hümeyra(18)
- Kizil, Hamit Emre(17)
- Semerci, Vahide(14)
- Bayram, Sinan(13)
- Kul, Murat(13)
- Sönmez Sarı, Ebru(13)
- Agar, Guleray(12)
- Akman, Onur(12)
- Kırbaş, Zila Özlem(12)
- Akeren, İhsan(11)
- Koçak, Lokman(11)
- Makas, Samet(11)
- Can, Zehra(10)
- Uzunçakmak, Sevgi Karabulut(10)
- Yayla, İbrahim Erdoğan(10)
- Çelik, Eyüp(10)
- Bayraktar, B.(9)
- Bulbuloglu, Semra(9)
- Kızıl, Hamit Emre(9)
- Tekce, Emre(9)
- Aydemir, Uğur(8)
- Daştan, Buket(8)
- Hintistan, Sevilay(8)
- Kalyoncuo, Servet(8)
- Sönmez Sari, Ebru(8)
- Türkmen, Mutlu(8)
- Apaydın, Emine(7)
- Arslaner, Ayla(7)
- Beydemir, Şükrü(7)
- Dombak, Kübra(7)
- Kara, Yakup(7)
- Mercan, Emin(7)
- Semerci Çakmak, Vahide(7)
- Can, Özgür Devrim(6)
- Esgin, Özge(6)
- Güneş, Hüseyin(6)
Yayınlar
- MakaleAçık Erişim2020Scopus SJRQ1Synthesis and characterisation of alternan-stabilised silver nanoparticles and determination of their antibacterial and antifungal activities against foodborne pathogens and fungi
Yilmaz, Mustafa Tahsin; İspirli, Hümeyra; Taylan, Osman; Dertli, Enes
In this study, alternan isolated from Lactobacillus reuteri E81 was used as the stabilising agent to produce alternan-stabilised silver nanoparticles (AgNPs-Alt) and AgNPs-Alt were characterized and their antibacterial and antifungal activities were tested. The formation and the stability of AgNPs-Alt were determined by UV–vis spectroscopy analysis. SEM and TEM analysis revealed the uniform morphology of AgNPs-Alt with an average size of ~50 nm. The crystalline nature of the AgNPs-Alt was confirmed by XRD analysis. The interactions of alternan functional groups with silver was observed by FTIR analysis and thermal characteristics of AgNPs-Alt were determined by DSC analysis. Finally, the antibacterial and the antifungal activities of AgNPs-Alt were tested and AgNPs-Alt revealed inhibitory effects to pathogenic strains at 0.25 mg ml−1 concentration and this activity was observed to be concentration dependent. For the antifungal activity, a higher level of AgNPs-Alt was required compared to the antibacterial activity.
AtıfScopus35WoS31OpenAlex36
İncele - MakaleAçık Erişim2020Scopus SJRQ2Bioactive Characteristics of Wild Berberis vulgaris and Berberis crataegina Fruits
Eroǧlu, Arzu Yaşar; Çakir, Özlem; Saǧdiç, Mustafa; Dertli, Enes
There is an increasing trend to find novel sources of products with high antioxidant capacity and wild fruits are very good examples for these sources. In this study, fruits of Berberis vulgaris and Berberis crataegina, naturally grown in Bayburt province of Turkey, were tested for their physicochemical features, antioxidant capacities, phenolic compound profiles, and antimicrobial activities. The physicochemical analysis of the fruits revealed that the dry matter content, ash content, pH, and aw values were between 28.47% and 41.61%, 0.65% and 2.13%, 2.44 and 3.25, and 0.996 and 0.97, respectively. The total phenolic content of the fruits was determined by the Folin-Ciocalteu methodology, and for the determination of the antioxidant capacity of the fruits, DPPH, ABTS, and β-carotene bleaching methods were performed and a high level of antioxidant activity was observed. HPLC analysis was applied to identify the phenolic content of the fruits, and gallic acid and chlorogenic acid were found to be the dominant phenolic compounds in Berberis fruits. The water extracts of the Berberis fruits were tested against important foodborne pathogenic bacteria as potential antimicrobials. The extracts inhibited the growth of Bacillus cereus, Salmonella typhimurium, Yersinia enterocolitica, and Staphylococcus aureus at significant rates. This study revealed the potential antioxidant and antimicrobial characteristics of wild-type B. vulgaris and B. crataegina that can be used for different future applications.
AtıfScopus36WoS27OpenAlex26İncele - Derleme MakaleYayıncıda açık erişim2020Functional Ice Cream Technology
Arslaner, Ayla; Salik, Mehmet Ali
Various factors besides changes in people’s lifestyle have altered the consumers understanding of dietary, health and welfare life. As a result, interest and demand for functional foods, which have been gaining momentum and importance in recent years, have increased day by day. Milk and dairy products have an important place among the functional foods considered as the foods of today and tomorrow. These products are very convenient in producing functional products due to their positive health effects in daily diet. Ice cream, which has a wide product distribution within this group, provides an advantage in the functional products market because it has a high nutritional value, its composition can be easily changed and it is a food consumed by individuals of all ages. This increases the number of studies on the functionalization of ice cream. When the studies conducted for this purpose were screened, it was determined that probiotic, prebiotic and symbiotic, antioxidant and phenolic compounds, bioactive protein-based components, whey and its products, various fatty acids like omega-3 and omega-6, some sweeteners such as stevia, honey and sugar alcohols, dietary fibers, some vitamins and minerals were used extensively in the literature. In this review, nutraceutical components commonly used in functional ice cream production technology are grouped and studies on functional ice cream are included.
AtıfScopus29OpenAlex24TR Dizin2
İncele - Derleme MakaleAçık Erişim2020Scopus SJRQ1Effects of umbelliferone isolated from the Ferulago pauciradiata Boiss. & Heldr. Plant on cecal ligation and puncture-induced sepsis model in rats
Kutlu, Z.; Celik, M.; Bilen, A.; Halıcı, Z.; Yıldırım, S.; Karabulut, S.; Karakaya, S.; Bostanlık, Delimustafaoğlu F.; Aydın, P.
Sepsis is a pathophysiological event involving systemic inflammatory response syndrome, multiple organ failure syndromes, and tissue damage. Overproduction of free radicals as a result of tissue damage during sepsis contributes to cellular toxicity, organ failure, and even mortality. Antioxidants, which scavenge free radicals, play a protective role against various diseases. Previous studies have shown that umbelliferone (UF) has antioxidant and anti-inflammatory effects. Since oxidative stress is naturally associated with sepsis-induced organ dysfunction, the application of antioxidant compounds could potentially illuminate the pathophysiology of sepsis, which does not yet have an effective treatment. The sepsis model induced by cecal ligation and puncture (CLP) was applied to rats. Different doses of UF (10░mg/kg, 20░mg/kg, and 40░mg/kg) on oxidant-antioxidant in septic rats, mRNA of inflammatory mediators such as tumor necrosis factor- α (TNF-α) and interleukin (IL)-1 its effects on expression levels were evaluated in lung, kidney, and liver tissues. When the lung, kidney, and liver tissues of septic rats were compared with those of the control group, it was found that UF administration increased dose-dependent superoxide dismutase activity and glutathione levels and significantly decreased malondialdehyde levels. The effects of UF administration on oxidative parameters were dose-dependent. The 40░mg/kg UF dose showed greater anti-oxidative properties than the 20░mg/kg and 10░mg/kg doses for all the evaluated parameters. Further, the TNF- α mRNA expression of the CLP +40░mg/kg group was reduced to a level comparable to that of the control group. UF has been found to be an effective molecule in reducing oxidative stress by supporting endogenous antioxidants and enhancing the scavenging effects of free radicals. The potent antioxidant property of UF may also be related to the suppression of the cytokine cascade during sepsis. The results suggest that UF administration may represent a new treatment for the prevention of lung, kidney and liver damage caused by septic conditions.
AtıfScopus22WoS21OpenAlex23
İncele - MakaleAçık Erişim2020Scopus SJRQ2Bifidogenic effect and in vitro immunomodulatory roles of melibiose-derived oligosaccharides produced by the acceptor reaction of glucansucrase E81
İspirli, Hümeyra; Kaya, Yasemin; Dertli, Enes
Glucansucrases are responsible for the production of α-glucans using sucrose as the substrate. Glucansucrases may also produce different oligosaccharides by transferring the glucose moiety from sucrose to a variety carbohydrates acting as acceptor nucleophile. In this study, melibiose-derived oligosaccharides were produced by the glucansucrase from Lactobacillus reuteri E81 expressed without the N-terminal region (GtfA-ΔN). The reaction products were characterized by TLC, LC-MS and NMR analysis and it was found that GtfA-ΔN synthesized melibiose-derived oligosaccharides (DP3 and DP4) by adding glucose units through alpha 1->3 or 1->6 glycosidic bond. The functional characteristics of these melibiose-derived oligosaccharides were determined by testing the immune-modulatory functions in HT-29 cells and testing their growth promoting effects for important probiotic and pathogenic strains. The melibiose-derived oligosaccharides triggered the production of anti-inflammatory cytokine IL-10 and pro-inflammatory cytokine TNF-α depending on their concentrations. Finally, melibiose-derived oligosaccharides showed bifidogenic effect as potential prebiotics.
AtıfScopus15WoS14OpenAlex15İncele - MakaleAçık Erişim2020Scopus SJRQ2Assessment of three mathematical prediction models for forecasting the covid-19 outbreak in Iran and Turkey
Niazkar, Majid; Türkkan, Gökçen Eryılmaz; Niazkar, Hamid Reza; Türkkan, Yusuf Alptekin
COVID-19 pandemic has become a concern of every nation, and it is crucial to apply an estimation model with a favorably-high accuracy to provide an accurate perspective of the situation. In this study, three explicit mathematical prediction models were applied to forecast the COVID-19 outbreak in Iran and Turkey. These models include a recursive-based method, Boltzmann Function-based model and Beesham's prediction model. These models were exploited to analyze the confirmed and death cases of the first 106 and 87 days of the COVID-19 outbreak in Iran and Turkey, respectively. This application indicates that the three models fail to predict the first 10 to 20 days of data, depending on the prediction model. On the other hand, the results obtained for the rest of the data demonstrate that the three prediction models achieve high values for the determination coefficient, whereas they yielded to different average absolute relative errors. Based on the comparison, the recursive-based model performs the best, while it estimated the COVID-19 outbreak in Iran better than that of in Turkey. Impacts of applying or relaxing control measurements like curfew in Turkey and reopening the low-risk businesses in Iran were investigated through the recursive-based model. Finally, the results demonstrate the merit of the recursive-based model in analyzing various scenarios, which may provide suitable information for health politicians and public health decision-makers.
AtıfScopus19WoS14OpenAlex24İncele - MakaleYalnız künye2020Scopus SJRQ4Screening for antioxidant capacity, pollen types and phytochemical profile by GC/MS and UHPLC from propolis
Bayram, Nesrin Ecem; Gerçek, Yusuf Can; Öz, Gül Cevahir
Propolis is a resinous mixture collected by honeybees from different parts of plants such as poplars, birches, alders, conifers, pines, palms and willows. This study aims to determine pollen types (by light microscope), chemical compound profile (by GC-MS), fatty acid composition (by GC-MS), total phenolic content (by the Folin-Ciocalteu method), total flavonoid content (by the aluminium chloride method) and antioxidant capacity (by the CUPRAC, ABTS and CERAC methods) of a propolis sample from the western part of Istanbul, Turkey. As a result of microscopic analysis of the sample, pollen types of taxa belonging to 27 plant families were diagnosed. The GC-MS analysis of propolis revealed the presence of 38 phytochemical constituents that may contribute to its quality. Of these compounds, rates of "4H-1-benzopyran-4-one, 2,3-dihydro-5,7-dihydroxy- 2-phenyl-,(S)"were highest. In addition, the concentrations of naringenin, pinocembrin and galangin were determined to be 2.45, 9.92 and 7.06 mg/ml by UHPLC analysis. The extract had significant antioxidant activity in all assays, with values of 282.8±9 mg TE /g in the CUPRAC, 425.7±18 mg TE/g in the CERAC and 186.4±8 mg TE/g in the ABTS assays. Antioxidant capacity of the propolis extract was positively associated with the total phenolic and flavonoid contents of the extract. Moreover, the major fatty acids were C20:1n9 (cis- 11-eicosenoic acid), C22:1 (erucic acid) and C24:1 (nervonic acid). The results show that the propolis used in our study has important potential as an alternative food supplement and for cosmetic and therapeutic medicine and it can be used as an active agent in these areas.
AtıfScopus20WoS18OpenAlex3İncele - Derleme MakaleAçık Erişim2020Scopus SJRQ2Avocado-Soybean Unsaponifiables: A Panoply of Potentialities to Be Exploited
Salehi, Bahare; Rescigno, Antonio; Dettori, Tinuccia; Calina, Daniela; Docea, Anca Oana; Singh, Laxman; Cebeci, Fatma; Özçelik, Beraat; Bhia, Mohammed; Dowlati Beirami, Amirreza
Avocado and soybean unsaponifiables (ASU) constitute vegetable extracts made from fruits and seeds of avocado and soybean oil. Characterized by its potent anti-inflammatory effects, this ASU mixture is recommended to act as an adjuvant treatment for osteoarthritic pain and slow-acting symptomatic treatment of hip and knee osteoarthritis; autoimmune diseases; diffuse scleroderma and scleroderma-like states (e.g., morphea, sclerodactyly, scleroderma in bands). Besides, it was reported that it can improve the mood and quality of life of postmenopausal women in reducing menopause-related symptoms. This article aims to summarize the studies on biological effects of the avocado-soybean unsaponifiable, its chemical composition, pharmacotherapy as well as applications in autoimmune, osteoarticular and menopausal disorders. Finally, we will also discuss on its safety, toxicological and regulatory practices.
AtıfScopus98WoS80OpenAlex103İncele - MakaleAçık Erişim2020Effect of dynamic and static strength training on hormonal activity in elite boxers
Aggön, Eser; Agirbas, Ozturk; Alp, Hamit Hakan; Uçan, Izzet; Gürsoy, Recep; Hackney, Anthony C.
Background: The aim of this study was to determine hormonal responses to acute and chronic exposure to static and dynamic strength training programs using resistance bands in boxers. Material and methods: 19 male national boxers participated in the study. Boxers were instructed to perform strength exercises with resistance bands for 3 days a week for 8 weeks involving either dynamic (n=10) or static (n=9) resistance exercises. Blood samples were taken before exercise, immediately after the initial exercise session, and 8 weeks later following the last exercise session. Cortisol, growth hormone, adrenocorticotropic hormone adrenaline and noradrenaline levels were measured. Statistical analyses involved non-parametric analysis with an alpha level of .05. Results: Dynamic strength exercises were effective stimuli to growth hormone, adrenaline and noradrenaline, while static strength exercises provoked cortisol, growth hormone, adrenaline and noradrenaline responses both initially after exercise and after 8 weeks of chronic training. Neither dynamic nor static strength exercises were effective in prompting adrenocorticotropic changes after an exercise session or after 8 weeks of training. Conclusions: We showed that dynamic and static strength exercise protocols using resistance bands both could provoke acute and chronic hormonal responses in boxers similar to more traditional modes of such exercise.
AtıfScopus5WoS6OpenAlex6İncele - MakaleAçık Erişim2019Analysis of genomic stability and DNA damage in plants exposed to cement dust pollution using the RAPD analysis
Ertürk, Filiz Aygün; Agar, Guleray; Sunar, Serap
One of the most important environmental pollutants is cement dust. In this study, RAPD technology was used todefine the potential genotoxic impact of cement dust on plants. For this purpose, the DNA comparison of 12 plant species(Convolvulus sepium, Astragalus christianus, Taraxacum androssovii Medicago varia, Alyssum murale, Artemisiaspisigera, Falcaria vulgaris, Anchusa strigose, Glaucium leiocarpum, Salvia syriaca, Cryciata taurica Tragopogonalbinervis)collected from the areas of 10000 m (Control area) or 0-100 m away from Askale cement factory in Erzurum;was performed. 16 primers were used, and 390 bands were obtained. Important differences were observed in plant RAPDprofiles collected from 0-100 m areas when compared to their respective controls (10000 m away from the factory). Somebands in control plants got lost, and new band formations were observed. The Genomic template stability (GTS) valuedecreased significantly in plants collected from 0-100 m area as compared to the controls (%56.20). Soil samples from 0-100 m and control area were collected and examined for their heavy metal contents. According to the analysis results,Nickel, Cadmium, Zinc, Lead and Copper concentrations were found to be dramatically high in the soil collected from 0-100 m as compared to the control area. These results indicate that the high heavy metal content in the soil causes thechanges in RAPD profiles and GTS in plants. The data also indicated that RAPD and GTS assays are basic, effective andreproducible means of the genotoxicity control of environmental pollution.
AtıfOpenAlex1İncele - MakaleAçık Erişim2019Functionally Graded Porous Implants Obtained by Additive Manufacturing
Murat, Fahri; Şensoy, Abdullah Tahir; Korkmaz, İsmail Hakkı; Kaymaz, Irfan
Porous structures have an increasing use in the biomedical industry due to their advantage, such as supporting tissue regeneration for implants and prostheses used in the body, eliminating stress shielding and aseptic loosening problems. Although porous structures with homogeneous density contributed positively to biological processes, these structures were insufficent to exhibit the desired mechanical properties. The implant to be used in this case should offer the optimum design both biologically and mechanically. In order to solve this problem, functionally graded structures which provide the harmony of tissue regeneration and mechanical behaviour on the load bearing orthopedic implant are presented. The design where porosity is very dense in the load-bearing sections of the implant and less dense in the areas in contact with the tissue have become feasible by the additive manufacturing methods. In this study, the recent developments in the literature on porous implants, functionally graded porous structures and additive manufacturing applications of these structures have been compiled. As a result, a procedure is presented on porous implant designs that will be used to improve mechanical properties by accelerating biological healing processes in additive manufacturing applications.
AtıfOpenAlex4TR Dizin3İncele - MakaleAçık Erişim2019Information age, digitalization and turkish health system
Alacadağlı, Esmeray
We are living in an age, which is also called information or digital revolution, where continuous learning and information are inevitable. On production, it is observed that many rules and principles which are used to get information, are replaced by raw materials, labor and capital. With its own rules and principles, there is now a new social and economic order. In order to survive and adapt to this system the necessity of having the knowledge and the conversions required by knowledge management. In the information society that replaces the industrial society, the informed individual becomes indispensable, while the organizational structures and management systems need to be redesigned in an individual-based manner. Organizations or institutions are structures established to achieve a certain purpose so that their surviving depends on the ability to answer the changing demands, adapt to the changes required by the age and provide services in a different way than others. In today's world, organizations that provide differentiation from other structures/competitors that provide services in this field are the means of benefiting from information and benefiting from it. The way of collecting, protecting and managing information also determines to lose (Özata, Sevinç, 2010:6-21). The aim of this study is to reveal the current status of e-health implementations in health sector and the effects of health service delivery as a public duty. With the literature review; it has been investigated how electronic information management, information systems and e-government applications that emerged in the mid-1990s affected and transformed health service delivery and management. In addition, qualitative analysis method was used because it was important in terms of verifying the information provided by literature review. In this context, health service providers and health information systems applicants were consulted and their opinions and suggestions were received.
AtıfOpenAlex14TR Dizin10İncele - MakaleAçık Erişim2019Functional Bioactive Components of Milk
Salik, Mehmet Ali; Arslaner, Ayla
Milk and dairy products are source of bioactive components that strengthen the immune system and also play a role in the regulation of many functional activities in the human organism. Some of these are casein, serum proteins, α-Lactoalbumin, β-lactaglobulin, serum albumin, immunoglobulins, glycoproteins, lactoferrin, lactoperoxidase and lysozyme; some lipid-based ingredients such as Docosahexaenoic Acid (DHA), Eicosapentaenoic Acid (EPA), Conjugated Linoleic Acid (CLA), phospholipids, sterols, vitamins and melatonin; minerals such as calcium and phosphorus. Serum proteins with high biological value from these components and protein and peptide fractions obtained by hydrolysis from these proteins are also used for enhancing nutritional value and improving the structural properties of foods and for enhancing food quality. In addition, the number of researches that are recommended to use foods such as lactoferrin, lactoperoxidase and lysozyme, which have natural antimicrobial properties in milk, and various fatty acids with antioxidant properties are increasing day by day. In this review, some bioactive components present in milk and their potential use in functional food production are discussed.
AtıfOpenAlex1TR Dizin1İncele - MakaleAçık Erişim2019Mükemmeliyetçilik ve Umutsuzluğun Akademik Erteleme Davranışı Üzerindeki Rolü: Üniversite Öğrencileri Üzerinde Bir Araştırma
Kaya, Feridun; Odaci, Hatice
Üniversite öğrencilerinin üniversite eğitimi süresince çoğunlukla yapması gereken akademik görevlerini ertelediği görülmektedir. Üniversite öğrencilerinin ödev yapma, proje yazma, derslere devam etme, sınavlara hazırlanma gibi durumlarına yönelik belirledikleri yüksek beklentiler, hata yapma endişeleri, ailesel beklentiler, aileden aldıkları eleştiri, yaptıklarından emin olamama ve düzenli olmaya yönelik düşünceleri veya geleceğe yönelik düşüncelerinde meydana gelen umutsuzluk onların akademik erteleme davranışını göstermesinde etkili olabilir. Bu bilgilerden hareketle bu çalışmanın amacı, üniversite öğrencilerinde mükemmeliyetçilik ile umutsuzluğun akademik erteleme davranışı üzerindeki rolünü araştırmaktır. Çalışma ilişkisel desene uygun olarak yürütülmüştür. Araştırmanın çalışma grubunu 2016-2017 akademik yılı güz döneminde Bayburt Üniversitesi’nde öğrenimlerine devam eden yaşları 18 ile 27 arasında değişen (x=19.96, Ss=1.50), 278 kadın (%57.4) ve 206 erkek (%42.6) toplam 484 üniversite öğrencisi oluşturmuştur. Araştırmada veri toplamak için Kişisel Bilgi Formu, Frost Çok Boyutlu Mükemmeliyetçilik Ölçeği, Akademik Erteleme Ölçeği ve Beck Umutsuzluk Ölçeği kullanılmıştır. Araştırma verilerinin analizinde Pearson korelasyon katsayısı tekniğinden ve çoklu doğrusal regresyon analizinden yararlanılmıştır. Yapılan analizler sonrasında; akademik erteleme ile; umutsuzluk (r=.24, p<.001), aile eleştirelliği (r=.20, p<.001) ve yaptığından emin olamama (r=.12, p<.01) arasında pozitif yönde anlamlı ilişkiler gözlenirken, kişisel standartlar (r=-.15, p<.01) ve düzen (r=-.40, p<.001) ile negatif yönde anlamlı ilişkiler tespit edilmiştir. Bununla birlikte elde edilen bulgular; aile eleştiriselliği (β=.12, p<.05), yaptığından emin olamama (β=.14, p<.05), kişisel standartlar (β=-.13, p<.05), düzen (β=-.34, p<.05) ve umutsuzluğun (β=.10, p<.05) akademik erteleme üzerindeki değişimin %22’sini açıkladığını göstermiştir (F(7,476) =18.95), p<.001). Çalışmanın sonuçları çok boyutlu mükemmeliyetçilik boyutlarının ve umutsuzluğun akademik erteleme ile ilişkili olduğunu göstermektedir.
AtıfOpenAlex12TR Dizin11İncele - MakaleAçık Erişim2019Tek Bir Seansta Zirve Oksijen Kullanım Düzeyini Veren En Yüksek Güç Çıktısını Tahmin Etmenin Basit Bir Yöntemi
Balcı, Görkem Aybars; Ozkaya, Ozgur; Norouzi, Mahdi; Çabuk, Refık; As, Hakan
Zirve O2 tüketim düzeyi (𝑉 O2pik) veren en yüksek güç çıktısı (aerobik limit güç; ALG)şiddetli ve aşırı egzersiz alanlarını ayıran önemli bir eşiktir ve sporcularda bireysel birALG belirleyebilmek için çok sayıda sabit yüklü tüketici test yapmak gerekmektedir.Bu çalışmanın amacı; sporcuların ALG’lerini tek bir seansta belirleyebilecek biryaklaşım önermektir. Araştırmaya iyi antrene 12 bisiklet sporcusu katılmıştır (𝑉 O2maks:64,7±4,2 mL∙dk-1∙kg-1; Zirve Güç (ZG): 374,1±65,7 W). 𝑉 O2pik düzeyinin belirlenmesiiçin lineer yük artışlı (ramp) testler uygulatılmıştır (+1 W·2 s-1) ve protokollerinsonunda ulaşılan final güç üretim değerleri (FG) ortalama yanıt zamanı (mean responsetime: MRT) düzeltmesi uygulanmadan (FGMRT) ve uygulanarak (FGMRT)değerlendirilmiştir. Sporcuların 𝑉 O2maks’ı ve bu değeri veren ZG değerleri, doğrulamatestleri uygulanarak bulunmuştur. Devamında sporcuların ALG (𝑉 O2maks’a %5’tendaha yakın 𝑉 O2 değeri veren en yüksek güç çıktısı) değerleri, 15 W’lık intervallerlebitkinlikle sonlanan sabit yüklü testler yoluyla ayrı günlerde taranmıştır. FGMRT ileALG değerleri arasındaki fark anlamlı değildir (FGMRT: 435,2±50,8; ALG: 435,4±62,5W, p=0,968). Bland-Altman sonuçları yüksek uyum göstermiştir (Bias=0,20±17,6 W;p=0,968). FGMRT, gerçek ZG’nin %117,5±8,8’ine karşılık gelirken, belirlenen ALGdeğeri ZG’nin %117,1±7,37’sine karşılık gelmiştir (p=0,759). Diğer yandan ramptestinden elde edilen en yüksek 15-s 𝑉 O2 ortalamaları da ALG yükündeki 𝑉 O2yanıtlarıyla benzerdir ( Ramp Test: 62,3±4,9 ml∙dk-1∙kg-1, %96,2; ALG: 61,5±4,3ml∙dk-1∙kg-1, %95; p=0,119 ). Sonuç olarak, ALG’yi belirleyebilmek için ayrı günlerdeçok sayıda bitkinlikle sonlanan test yapmak yerine, tek seansta bir ramp testuygulamasının 𝑉 O2maks’ı düşük tahmin ettiği ancak ALG’yi ve bu değere karşılık gelen𝑉 O2 yanıtını belirleyebilmenin oldukça pratik bir yöntemi olabileceği bulunmuştur
AtıfTR Dizin1İncele - MakaleAçık Erişim2019Scopus SJRQ1Characterization of a 4,6‑α‑glucanotransferase from Lactobacillus reuteri E81 and production of malto-oligosaccharides with immune-modulatory roles
İspirli, Hümeyra; Şimşek, Ömer; Skory, Christopher; Sağdıç, Osman; Dertli, Enes
A wide number of Lactic Acid Bacteria (LAB) species produce α-glucans with their ability to synthesize glucansucrases (GS) which use sucrose as substrate for the glucan production. Recently another group of enzymes in LAB gained special interest for their ability to produce α-glucans targeting the substrates containing α1-4-linkages and synthesizing new (α1-6) or (α1-3)–linkages as α‑glucanotransferases. In this study, a putative 4,6‑α‑glucanotransferase (GTFB) from sourdough isolate Lactobacillus reuteri E81 was identified and expressed in Escherichia coli. The biochemical characterization of the GTFB-E81 confirmed its function as it cleaved the α1-4-linkages in different substrates and produced new gluco-oligomers/polymers containing α1-6 linkages together with the α1-4-linkages detected by NMR analysis. GTFB-E81 produced malto-oligosaccharides targeting maltose and maltoheptaose as substrates with up to DP 8 detected by TLC and ESI-MS/MS analysis. The functional roles of these malto-oligosaccharides were determined by testing their immune-modulatory functions in HT29 cells and they triggered the production of anti-inflammatory 1L-4 and pro-inflammatory IL-12 cytokines.
AtıfScopus43WoS40OpenAlex42İncele - MakaleAçık Erişim2019Scopus SJRQ1Electrospraying method for fabrication of essential oil loaded-chitosan nanoparticle delivery systems characterized by molecular, thermal, morphological and antifungal properties
Yilmaz, Mustafa Tahsin; Yilmaz, Azime; Akman, Perihan Kubra; Bozkurt, Fatih; Dertli, Enes; Basahel, Abdulrahman; Al-Sasi, Basil; Taylan, Osman; Sagdic, Osman
Fabrication of essential oil loaded-chitosan nanoparticles using electrospraying technique appears to be a novel strategy to develop thermally stable nanoparticles possessing higher encapsulation efficiency and particle stability. This study aims to fabricate chitosan nanoparticles (CNPs) loaded with Origanum vulgare essential oil (OEO, Origanum vulgare L.) at different proportions (OEO/CH proportions of 0:1, 0.0625:1, 0.125:1, 0.25:1 and 0.5:1 mL/g) using electrospraying technique. The CNPs were characterized in term of their particle size and stability (dynamic light scattering), encapsulation efficiency (spectrophotometry), and molecular (Fourier transform infrared spectroscopy), thermal (differential scanning calorimetry/thermogravimetric analysis), morphological (scanning electron microscopy) and antifungal (agar dilution method) and fungistatic activity properties. The average particle sizes of the CNPs ranged between 290 and 483 nm with a spherical morphology. Positively charged surface characteristics were observed to increase with the increment of OEO concentration in CNPs. The encapsulation efficiency values were determined in the range of 70.1 and 79.6%. The molecular and thermal analyses exposed very decent encapsulation of OEO into thermally stable chitosan nanoparticles. Morphological analysis verified the spherical shapes of these nanoparticles. Above all, the antifungal effectiveness of OEO against the Alternaria alternata AY1 could be significantly (p < 0.05) increased by its encapsulation into chitosan nanoparticles fabricated by the electrospraying technique. Consequently, it can be stated that the electrospraying technique developed is able to fabricate thermally stable nanoparticles owning higher encapsulation efficiency and particle stability. The results and findings suggest that the electrospraying technique would be a promising method to fabricate chitosan-based nanoparticles as an antimicrobial agent to control their release in a prolonged preservative effect in cosmetic, pharmaceutical and food applications for adjustable dosage forms. Industrial relevance: The fungal agents such as Alternaria alternata cause great damages on post-harvest fresh fruits and vegetables, thus leading to a great economical lose. Therefore, a great variety of methods in struggling with disease have been previously proposed. Nowadays, the most effective leading methods to struggle against plant diseases are those applied with synthetic fungicides to minimize such lose in post-harvest fruit and vegetables. However, intensive and unconscious use of the fungicides leads the pathogens to develop resistance against these agents as well as to accumulation of chemical residues in soil, water and air and finally to formation of carcinogenic effects on human health. Nowadays, a great effort is being exerted to develop novel biodegradable and natural antimicrobial agents for struggling fungal spoilage in postharvest products. Essential oils are among the most widely used natural struggling methods. Essential oils obtained from plants have been extensively used since they are natural antimicrobial agents. However, they cannot be effectively used in spite of their extensive applications. Recently, some researches in the field of nanotechnology have demonstrated that the effectiveness of active substances could be increased by using some techniques. In this respect, we aimed at developing essential-oil-loaded-chitosan-nanoparticle delivery systems using an electrospraying deposition system to prevent fungal colonization on food and plant materials. By increasing antifungal effectiveness of essential oils by their encapsulation into nanoparticles, it will be possible to decrease the levels of regularly applied dose and reflect the obtained outcomes to the food and agriculture industry. This study is the first example of production of essential oil loaded nanoparticles using the electrospraying-hydrodynamic process and showed that encapsulation of oregano essential oil into chitosan based nanoparticles (CNPs) by using the electrospraying deposition technique considerably increased the antifungal effectiveness of the Origanum vulgare essential oil. Our results highlight the potential use of the chitosan nanoparticles (CNPs) loaded with different amounts of Origanum vulgare in food and agriculture industry as an effective fungicidal material against Alternaria alternata, suggesting that the CNPs can be promising tools to compete with synthetic fungicide counterparts and limit use of synthetic ones for struggling of food and plant pathogens. Therefore, the results of this study should be of great importance to industrial applications in terms of development of natural, but effective preservatives as alternative to synthetic ones. In this respect, the CNPs would find a great industrial application area in the food and agriculture industry which seek natural preservatives due to the recent health concerns.
AtıfScopus119WoS101OpenAlex118İncele - MakaleAçık Erişim2019Scopus SJRQ2Employment of emergency advanced nurses of Turkey: A discrete-event simulation application
Atalan, Abdulkadir; Donmez, Cem Cagri
In the present study, problems in emergency services (ESs) were dealt with by analyzing the working system of ESs in Turkey. The purpose of this study was to reduce the waiting times spent in hospitals by employing advanced nurses (ANs) to treat patients who are not urgent, or who may be treated as outpatients in ESs. By applying discrete-event simulation on a 1/24 (daily) and 7/24 (weekly) basis, and by employing ANs, it was determined that the number of patients that were treated increased by 26.71% on a 1/24 basis, and by 15.13% on a 7/24 basis. The waiting time that was spent from the admission to the ES until the treatment time decreased by 38.67% on a 1/24 basis and 53.66% on a 24/7 basis. Similarly, the length of stay was reduced from 82.46 min to 53.97 min in the ES. Among the findings, it was observed that the efficiency rate of the resources was balanced by the employment of ANs, although it was not possible to obtain sufficient efficiency from the resources used in the ESs prior to the present study.
AtıfScopus9WoS8OpenAlex13İncele - MakaleYalnız künye2019Scopus SJRQ1Effect of electrospun nisin and curcumin loaded nanomats on the microbial quality, hardness and sensory characteristics of rainbow trout fillet
Meral, Raciye; Alav, Aslıhan; Karakas, CananYagmur; Dertli, Enes; Yilmaz, Mustafa Tahsin; Ceylan, Zafer
Nisin and curcumin loaded (NCL) nanomats having 172 nm mean diameter were successfully fabricated. The effect of NCL nanomats in terms of limitation of Total Mesophilic Aerobic (TMAB) and Lactic acid bacteria (LAB) in fish fillets was tested during the analysis period. On the 4th day, while TMAB load of C was 6.61 log CFU g−1, that of the sample coated with NCL nanomats was found to be 3.28 log CFU g−1. TMAB load of control samples reached above 6 log CFU g−1 on the 4th day of cold storage, TMAB load of fish samples coated with nanomats reached the same level up to the 12th day. The shelf life of coated fillets was extended to 12 day. 3–4 logarithmic decrease was obtained in LAB count by NCL nanomats during the analysis period. On the 4th storage day, the hardness value of C sample decreased 21% compared to NCL samples. All sensory attributes of C samples were acceptable by the 4th day of the storage; while sensory attributes of NCL samples were acceptable by the 10th day of storage. The study revealed that nisin and curcumin loaded nanomats could be effectively used to improve the quality of fish fillets.
AtıfScopus92WoS84OpenAlex91İncele - MakaleAçık Erişim2019Scopus SJRQ2Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions
İspirli, Hümeyra; Colquhoun, Ian J.; Şahin, Engin; Sagdic, Osman; Dertli, Enes
Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
AtıfScopus26WoS21OpenAlex22İncele
