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Preparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions

MakaleAçık ErişimScopus SJRQ2
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Özet

Gentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.

Anahtar Kelimeler

Gentiobiose-derived oligosaccharidesGlucansucraseImmune modulationPrebiotic
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İndeks kayıtları
ScopusScopusWeb of ScienceWeb of SciencePubMedPubMed
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Scopus
26
WoS
21
OpenAlex
22
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorİspirli, Hümeyra
dc.contributor.authorColquhoun, Ian J.
dc.contributor.authorŞahin, Engin
dc.contributor.authorSagdic, Osman
dc.contributor.authorDertli, Enes
dc.date.accessioned2026-10-29T19:11:08.120101098Z
dc.date.available2026-10-29T19:11:08.120101098Z
dc.date.issued2019
dc.description.abstractGentiobiose-derived oligosaccharides were synthesized by the acceptor reaction of glucansucrase E81 obtained from Lactobacillus reuteri E81 with sucrose and gentiobiose as donor-acceptor sugars, respectively. The reaction products were monitored by TLC analysis and gentiobiose-derived oligosaccharides up to DP 8 were formed during the acceptor reaction as determined by ESI-MS/MS analysis. The glycosylation of the gentiobiose with α-(1 → 6) linkages and α-(1 → 3) linkages was shown by 1H and 13C NMR analysis confirming the structure of these gentiobiose-derived oligosaccharides. The in vitro prebiotic function of the oligosaccharides was determined in which probiotic strains were stimulated whereas no growth was observed in pathogen strains. Gentiobiose-derived oligosaccharides showed immune-modulatory functions in vitro and triggered the production of IL-4, IL12 and TNF-α cytokines in HT29 cells in a dose dependent manner. This study showed the production and functional characterisation of gentiobiose-derived oligosaccharides establishing a promising avenue for future applications.
dc.description.sponsorshipTUBİTAK, (116O523)
dc.identifier.articlenumber107837
dc.identifier.doi10.1016/j.carres.2019.107837
dc.identifier.eissn1873-426X
dc.identifier.issn0008-6215
dc.identifier.pmid31655418
dc.identifier.scopus2-s2.0-85073676607
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/bayburt/13550
dc.identifier.volume486
dc.identifier.wosWOS:000496174300018
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofCarbohydrate Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGentiobiose-derived oligosaccharides
dc.subjectGlucansucrase
dc.subjectImmune modulation
dc.subjectPrebiotic
dc.subject.classificationBiochemistry & Molecular Biology
dc.subject.classificationChemistry, Applied
dc.subject.classificationChemistry, Organic
dc.subject.classificationChemistry
dc.subject.otherDisaccharides
dc.subject.otherGlycosyltransferases
dc.subject.otherHT29 Cells
dc.subject.otherHumans
dc.subject.otherImmunologic Factors
dc.subject.otherPrebiotics
dc.subject.otheralternansucrase
dc.subject.otherdisaccharide
dc.subject.othergentiobiose
dc.subject.otherglycosyltransferase
dc.subject.otherimmunologic factor
dc.subject.otherprebiotic agent
dc.subject.otherchemistry
dc.subject.otherHT-29 cell line
dc.subject.otherhuman
dc.subject.othermetabolism
dc.titlePreparation of gentiobiose-derived oligosaccharides by glucansucrase E81 and determination of prebiotic and immune-modulatory functions
dc.typeArticle
rdl.citation.openalex22
rdl.citation.scopus26
rdl.citation.wos21
rdl.departmentYıldız Technical University, Chemical and Metallurgical Engineering Faculty, Department of Food Engineering, Istanbul, 34000, Turkey
rdl.departmentAnalytical Sciences Unit, Quadram Institute Bioscience, Norwich, UK
rdl.departmentDepartment of Food Engineering, Faculty of Engineering, Bayburt University, Bayburt, 69000, Turkey
rdl.oa.statusclosed
rdl.oa.statusgreen
rdl.oa.urlhttp://hdl.handle.net/20.500.12403/3616
rdl.scopus.quartileQ2
rdl.sdgSDG03
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)