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Production of mannose-containing oligosaccharides by glucansucrase E81 and determination of their functional characteristics

MakaleYalnız künyeScopus SJRQ3
  • SDG 3 · Sağlık ve Kaliteli Yaşam

Özet

Oligosaccharides are one of the functional ingredients to be used in food technology. In this study, by using an active glucansucrase GTFA-ΔN E81 in the acceptor reaction of mannose, mannose-containing oligosaccharides were produced and their functionality was tested. The formation of the oligosaccharides were visualised by TLC analysis and mannose-containing oligosaccharides up to DP 7 were determined by LC-MS analysis. The presence of the (1,6)Glc and (1,3)Glc units within the oligosaccharides were determined by NMR analysis. The in vitro immune-modulatory functions of the mannose-containing oligosaccharides were determined but no induction in IL-4, IL-10, IL-12 and TNF-α cytokine levels were detected. Importantly this oligosaccharide mixture showed prebiotic effect by triggering the growth of tested probiotics and not affecting the growth of pathogen strains. Our findings reveals the potential of the role of glucansucrases for the production of functional oligosaccharides.

Anahtar Kelimeler

Glucansucraseimmune modulationmannose-containing oligosaccharidesprebiotic
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İndeks kayıtları
ScopusScopusWeb of ScienceWeb of Science
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Atıflar
Scopus
8
WoS
7
OpenAlex
8
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorİspirli, Hümeyra
dc.contributor.authorDertli, Enes
dc.date.accessioned2026-10-29T19:10:59.269869371Z
dc.date.available2026-10-29T19:10:59.269869371Z
dc.date.issued2020
dc.description.abstractOligosaccharides are one of the functional ingredients to be used in food technology. In this study, by using an active glucansucrase GTFA-ΔN E81 in the acceptor reaction of mannose, mannose-containing oligosaccharides were produced and their functionality was tested. The formation of the oligosaccharides were visualised by TLC analysis and mannose-containing oligosaccharides up to DP 7 were determined by LC-MS analysis. The presence of the (1,6)Glc and (1,3)Glc units within the oligosaccharides were determined by NMR analysis. The in vitro immune-modulatory functions of the mannose-containing oligosaccharides were determined but no induction in IL-4, IL-10, IL-12 and TNF-α cytokine levels were detected. Importantly this oligosaccharide mixture showed prebiotic effect by triggering the growth of tested probiotics and not affecting the growth of pathogen strains. Our findings reveals the potential of the role of glucansucrases for the production of functional oligosaccharides.
dc.description.sponsorshipTUBITAK [116O523]
dc.identifier.doi10.1080/10242422.2019.1661384
dc.identifier.eissn1029-2446
dc.identifier.endpage209
dc.identifier.issn1024-2422
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85072023029
dc.identifier.startpage202
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/bayburt/13455
dc.identifier.volume38
dc.identifier.wosWOS:000485975200001
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofBiocatalysis and Biotransformation
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGlucansucrase
dc.subjectimmune modulation
dc.subjectmannose-containing oligosaccharides
dc.subjectprebiotic
dc.subject.classificationBiochemistry & Molecular Biology
dc.subject.classificationBiotechnology & Applied Microbiology
dc.subject.otherGas chromatography
dc.subject.otherProbiotics
dc.subject.otherglucansucrase e81
dc.subject.otherinterleukin 10
dc.subject.otherinterleukin 12
dc.subject.otherinterleukin 4
dc.subject.othermannose
dc.subject.otheroligosaccharide
dc.subject.otherprebiotic agent
dc.subject.otherprobiotic agent
dc.subject.othersucrase
dc.subject.othersucrose
dc.subject.othertumor necrosis factor
dc.subject.otherunclassified drug
dc.subject.otherFunctional characteristics
dc.subject.otherFunctional ingredient
dc.subject.otherGlucansucrase
dc.subject.otherImmune modulation
dc.subject.otherImmune modulatory functions
dc.subject.otherLc-ms analysis
dc.subject.otherPrebiotic effect
dc.subject.otherPrebiotics
dc.subject.otherArticle
dc.subject.otherBacillus cereus
dc.subject.otherbacterial growth
dc.subject.otherbacterial strain
dc.subject.otherBifidobacterium animalis
dc.subject.otherBifidobacterium breve
dc.subject.otherBifidobacterium longum
dc.subject.otherbiotechnological production
dc.subject.othercarbohydrate synthesis
dc.subject.othercarbon nuclear magnetic resonance
dc.subject.othercontrolled study
dc.subject.othercytokine production
dc.subject.otherelectrospray mass spectrometry
dc.subject.otherEscherichia coli
dc.subject.otherhuman
dc.subject.otherhuman cell
dc.subject.otherimmunomodulation
dc.subject.otherin vitro study
dc.subject.otherLactobacillus plantarum
dc.subject.otherLactobacillus reuteri
dc.subject.otherLactobacillus rhamnosus
dc.subject.otherliquid chromatography-mass spectrometry
dc.subject.othernonhuman
dc.subject.otherproton nuclear magnetic resonance
dc.subject.otherSalmonella enterica serovar Typhimurium
dc.subject.otherStaphylococcus aureus
dc.subject.othertandem mass spectrometry
dc.subject.otherYersinia enterocolitica
dc.subject.otherOligosaccharides
dc.titleProduction of mannose-containing oligosaccharides by glucansucrase E81 and determination of their functional characteristics
dc.typeArticle
rdl.citation.openalex8
rdl.citation.scopus8
rdl.citation.wos7
rdl.oa.statusclosed
rdl.scopus.quartileQ3
rdl.sdgSDG03
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)