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  • MakaleYalnız künye2013Scopus SJRQ3
    The effects of reading-writing-application technique and learning together technique on increasing 6th grade students' academic achievement and students' opinions about these techniques

    Aksoy, Gokhan; Gurbuz, Fatih

    The aim of this study is to identify the effects of Reading-Writing-Application technique, Learning Together technique and Traditional Teaching methods on increasing students' academic achievements in the unit "particular structure of matter" at the science and technology course. In addition, students' written opinions were received from students (RWAG and LTG) about Reading-Writing-Application and Learning Together techniques. This study included 92 students from three different classes at the 6 th grade of a primary school during the 2010-2011 academic year. One of these classes served as the Reading-Writing-Application Group (RWAG), using group Reading-Writing-Application Technique, the second one served as the Learning Together Group (LTG), using the Learning Together technique, and the last one served as the Traditional Teaching Group (TTG), using the Traditional Teaching methods. The main instruments for obtaining data were the Academic Achievement Test (AAT) and Students' Written Opinions (SWO). Academic Achievement Test was applied to the all treatments groups but Students' Written Opinions was only applied RWAG and LTG. The data obtained on instruments were evaluated by using descriptive statistic, one-way variance analysis ANOVA and content analysis according to qualitative approach. It was concluded from the research results that the teaching of the unit "particular structure of matter" via the Reading-Writing-Application technique was more effective in increasing students' academic achievements compared to Learning Together technique and the Traditional Teaching methods. In addition, the written opinions of RWAG students and LTG students showed that the students have some negative opinions about Cooperative Learning Method.

    AtıfScopus2
    Scopusİncele
  • MakaleAçık Erişim2013Scopus SJRQ1
    Structure and biosynthesis of two exopolysaccharides produced by lactobacillus johnsonii FI9785

    Dertli, Enes; Colquhoun, Ian J.; Gunning, A. Patrick; Bongaerts, Roy J.; Le Gall, Gwénaëlle; Bonev, Boyan B.; Mayer, Melinda J.; Narbad, Arjan

    Exopolysaccharides were isolated and purified from Lactobacillus johnsonii FI9785, which has previously been shown to act as a competitive exclusion agent to control Clostridium perfringens in poultry. Structural analysis by NMR spectroscopy revealed that L. johnsonii FI9785 can produce two types of exopolysaccharide: EPS-1 is a branched dextran with the unusual feature that every backbone residue is substituted with a 2-linked glucose unit, and EPS-2 was shown to have a repeating unit with the following structure: -6)-α-Glcp-(1-3)-β-Glcp-(1-5)-β-Galf-(1-6)-α-Glcp-(1-4) -β-Gal p-(1-4)-β-Glcp-(1-. Sites on both polysaccharides were partially occupied by substituent groups: 1-phosphoglycerol and O-acetyl groups in EPS-1 and a single O-acetyl group in EPS-2. Analysis of a deletion mutant (δepsE) lacking the putative priming glycosyltransferase gene located within a predicted eps gene cluster revealed that the mutant could produce EPS-1 but not EPS-2, indicating that epsE is essential for the biosynthesis of EPS-2. Atomic force microscopy confirmed the localization of galactose residues on the exterior of wild type cells and their absence in the δepsE mutant. EPS2 was found to adopt a random coil structural conformation. Deletion of the entire 14-kb eps cluster resulted in an acapsular mutant phenotype that was not able to produce either EPS-2 or EPS-1. Alterations in the cell surface properties of the EPS-specific mutants were demonstrated by differences in binding of an anti-wild type L. johnsonii antibody. These findings provide insights into the biosynthesis and structures of novel exopolysaccharides produced by L. johnsonii FI9785, which are likely to play an important role in biofilm formation, protection against harsh environment of the gut, and colonization of the host.

    AtıfScopus131WoS120OpenAlex134
    ScopusWeb of SciencePubMedİncele
  • MakaleAçık Erişim2013Scopus SJRQ2
    Experimental research of dynamic instabilities in the presence of coiled wire inserts on two-phase flow

    Omeroglu, Gokhan; Comakli, Omer; Karagoz, Sendogan; Sahin, Bayram

    The aim of this study is to experimentally investigate the effect of the coiled wire insertions on dynamic instabilities and to compare the results with the smooth tube for forced convection boiling. The experiments were conducted in a circular tube, and water was used as the working fluid. Two different pitch ratios (H / D = 2.77 and 5.55) of coiled wire with circular cross-sections were utilised. The constant heat flux boundary condition was applied to the outer side of the test tube, and the constant exit restriction was used at the tube outlet. The mass flow rate changed from 110 to 20 g/s in order to obtain a detailed idea about the density wave and pressure drop oscillations, and the range of the inlet temperature was 15-35°C. The changes in pressure drop, inlet temperature, amplitude, and the period with mass flow rate are presented. For each configuration, it is seen that density wave and pressure drop oscillations occur at all inlet temperatures. Analyses show that the decrease in the mass flow rate and inlet temperature causes the amplitude and the period of the density wave and the pressure drop oscillations to decrease separately.

    AtıfScopus2WoS1OpenAlex4
    ScopusWeb of SciencePubMedİncele
  • MakaleYalnız künye2013Scopus SJRQ2
    Determination of conformational and spectroscopic features of ethyl trans-alfa-cyano-3-indole-acrylate compound: An experimental and quantum chemical study

    Cinar, Mehmet; Karabacak, Mehmet

    The optimized geometrical structure, vibrational and electronic transitions, chemical shifts and non-linear optical properties of ethyl trans-alfa-cyano-3-indole-acrylate (C14H12N 2O2) compound were presented in this study. The ground state geometrical structure and vibrational wavenumbers were carried out by using density functional (DFT/B3LYP) method with 6-311++G(d,p) as basis set. The vibrational spectra of title compound were recorded in solid state with FT-IR and FT-Raman in the range of 4000-400 cm-1 and 4000-10 cm -1, respectively. The fundamental assignments were done on the basis of the total energy distribution (TED) of the vibrational modes, calculated with scaled quantum mechanical (SQM) method. The 1H, 13C and DEPT NMR spectra were recorded in DMSO solution, and gauge-invariant atomic orbitals (GIAO) method was used to predict the isotropic chemical shifts. The UV-Vis absorption spectra of the compound were recorded in the range of 200-800 nm in various solvents of different polarity (acetone, benzene, chlorobenzene, chloroform, DMSO, ethanol, methanol and toluene). Solvent effects were calculated using TD-DFT and CIS method. To investigate the non-linear optical properties, the polarizability, anisotropy of polarizability and molecular first hyperpolarizability were computed. A detailed description of spectroscopic behaviors of compound was given based on the comparison of experimental measurements and theoretical computations.

    AtıfScopus12WoS11
    ScopusWeb of SciencePubMedİncele
  • MakaleAçık Erişim2013Scopus SJRQ2
    An experimental and theoretical investigation of Acenaphthene-5-boronic acid: Conformational study, NBO and NLO analysis, molecular structure and FT-IR, FT-Raman, NMR and UV spectra

    Karabacak, Mehmet; Sinha, Leena; Prasad, Onkar; Asiri, Abdullah M.; Cinar, Mehmet

    The solid state Fourier transform infrared (FT-IR) and FT-Raman spectra of Acenaphthene-5-boronic acid (AN-5-BA), have been recorded in the range 4000-400 cm1 and 4000-10 cm 1, respectively. Density functional theory (DFT), with the B3LYP functional was used for the optimization of the ground state geometry and simulation of the infrared and Raman spectra of the molecule. The vibrational wave numbers and their assignments were examined theoretically using the Gaussian 09 set of quantum chemistry codes and the normal modes were assigned by a scaled quantum mechanical (SQM) force field approach. Hydrogen-bonded dimer of AN-5-BA, optimized by counterpoise correction, has also been studied by B3LYP at the 6-311++G(d,p) level and the effects of molecular association through O-H·O hydrogen bonding have been discussed. The 1H and 13C nuclear magnetic resonance (NMR) chemical shifts of the molecule were calculated by Gauge-Including Atomic Orbital (GIAO) method. Natural bond orbital (NBO) analysis has been applied to study stability of the molecule arising from charge delocalization. UV spectrum of the title compound was also recorded and the electronic properties, such as frontier orbitals, and band gap energies were measured by TD-DFT approach. The first order hyperpolarizability (β), its components and associated properties such as average polarizability and anisotropy of the polarizability (α and Δα) of AN-5-BA was calculated using the finite-field approach.

    AtıfScopus38WoS36OpenAlex33
    ScopusWeb of SciencePubMedİncele
  • MakaleYalnız künye2012
    An approach based on TRIZ methodology and SERVQUAL scale to improve the quality of health-care service: a case study

    Yener, Engin; Altuntas, Serkan

    Sağlık hizmetlerinin kalitesini arttırmak için yaratıcı problem çözme teorisi (TRIZ) ve hizmet kalitesi ölçüm skalasına (SERVQUAL) dayanan yeni bir yenilikçi yaklaşım önerilmiştir. SERVQUAL servis kalitesinin ölçümü için (özellikle sağlık hizmetlerinde) yaygın şekilde kullanılırken, TRIZ, sanayileşmiş ülkelerde sorunların çözümü için geniş bir alanda kullanılmaktadır. Su et al. (2008), servis kalitesi ile ilgili problemlerin çözümü için TRIZ’e dayanan sistematik bir çerçeve önermiştir. Bu çalışmada ise, SERVQUAL skalasının parametrelerini TRIZ’e adapte ederek, sağlık hizmetleri sektörünün kalitesini arttırmak için Su et al. (2008)’ün çalışması ışığında bir metodoloji önerilmektedir. İlk olarak TRIZ’in 39 mühendislik parametresinin SERVQUAL skalası ile benzeşen boyutları eşleştirilmiştir. Sonra çalışmamızı doğrulamak için anket formu hazırlanmıştır. Anket formumuz, Türkiye’de, Atatürk Üniversitesi Hastanesindeki on doktora(3’ü profesör diğerleri uzman doktor) uygulanmıştır. Son olarak, bu yeni yenilikçi yaklaşımın nasıl uygulanacağını göstermek için bir örnek uygulama yürütülmüştür. TRIZ’in uygulama alanı bu çalışma sayesinde genişletilmiştir.

    AtıfTR Dizin2
    TR Dizinİncele
  • MakaleYalnız künye2012
    Effects of two different cooperative learning techniques on students’ academic achievements

    Aksoy, Gokhan; Gurbuz, Fatih

    The aim of this study is to determine the effects of two different cooperative learning techniques (Student Teams-Achievement Divisions technique and Jigsaw technique) on academic achievement of seventh grade students at unit of the “Electricity in Our Life”. The subjects of the study were 62 seventh grade students at a primary school in Erzurum, who were in two different classes and taught by the same teacher at 2011-2012 education year. One of the classes was randomly selected as the Student Teams-Achievement Divisions Group (STADG), using group student teams-achievement divisions technique and the second served as the Jigsaw Group (JG), using group jigsaw technique. The main instruments for obtaining data were the Electricity in Our Life Academic Achievement Test (EOLAAT). The study results were analyzed by SPSS. The data obtained on instruments were evaluated by using descriptive statistic, independent samples groups t test, paired sample groups t test and effect sizes. As the results of the EOLAAT revealed that student teams-achievement divisions technique is more successful than jigsaw technique in the unit “electricity in our life”.

    AtıfTR Dizin8
    TR Dizinİncele
  • MakaleYalnız künye2012
    Endülüs’te Tefsir kültürü

    Şentürk, Mustafa

    Endülüs tefsir kültürünü ele almayı amaçladığımız bu makalede, bölgede oluşan tefsîr anlayışını genel hatlarıyla şu sorulara cevap arayarak ele almak istiyoruz: Endülüs’te tefsîr nasıl ve ne zaman başladı? Endülüs’te tefsîrin öncüleri kimlerdi? Endülüs’te tefsîrin kaynakları nelerdi? Bölge müfessir-leri eserlerinde daha çok hangi Tefsîr kitaplarına başvuruyorlardı? Bölgede yaygın olan tefsîr usûlü nasıldı? Çeşitli tefsîr yönelişlerinden hangilerine daha çok ilgi duyuyorlardı? Bölge tefsîrinin özellikle-ri nelerdi? Endülüs’teki tefsîr anlayışı, diğer İslâm bölgelerinden farklılık arz ediyor muydu? Arz ediyor idiyse bunlar nelerdi? Ve son olarak Endülüs, bütün yönleriyle tefsîrde bir ekol oluşturabildi mi?

    AtıfTR Dizin6
    TR Dizinİncele
  • MakaleYalnız künye2012
    Demand for information and communication technology: An application

    Kaynak, Selahattin; Karaca, Zeynep

    Information and communication technologies are increasingly permeates daily life. The use of information and communication technologies that significantly affects the economic behavior of all units of economic decision is increasing day by day. For this reason, which information and communication technology tools and how often they are used and the identification of factors which affect their choice and demand for these tools are so important. In this study prepared for achieving these objectives, exploratory factor analysis, ANOVA and t test analysis was performed. The results of analysis show that as the income level, education level and number of employees in the family increases, the demand for information and communication technologies are increasing, too. At the same time, it is concluded that young people, people living in the city center and men demand information and communication technologies more.

    TR Dizinİncele
  • MakaleYalnız künye2012
    Health services demand: A Study in Erzurum

    Kaynak, Selahattin; Yaylali, Muammer; Karaca, Zeynep

    The sector of health services is a sector with a high potential of improvement. Given that every individual is likely to come across health services and that the resources shared for health services constitute a high rate of the resources shared for social welfare and since it is a sector with high externalities, it is of great importance to research the demand for this sector. The aim of this study, in line with the importance of this sector, is to determine the factors affecting the demand for health services. For this purpose, we estimate a logit model of demand for health services with SPSS and LIMDEP package softwares using the survey data. Our analysis suggests that the probability of the consumers demand for health services is positively related to the income, age and the number of the individuals in a family. It is also found that the average of the group specific variables, i.e. the economic factors, the attitudes and behaviors of the medical officials and the physical environments of the hospitals are significantly different from the general average in statistical terms at 0.05 level of significance.

    AtıfTR Dizin16
    TR Dizinİncele
  • MakaleYalnız künye2012
    Çatışma yönetim stratejisinin tahmin edicileri olarak örgütsel kültür tipleri

    Kahya, Cem; Buzkan, Kemal; Karcıoğlu, Fatih

    Her örgütü karakterize eden özellikler farklıdır ve bu farklılığın asıl kaynaklarından birisi de, örgütsel kültürdür. Her örgüt, kendine özgü bir kültür anlayışına sahiptir. Bu nedenle örgüt içerisinde ortaya çıkan çatışmanın yönetilmesinde, örgütsel kültürün de dikkate alınması gerekecektir. Buradan hareketle çalışmamızın temel amacı, örgüt yöneticileri tarafından çoğunlukla tercih edilen çatışma yönetim stratejisi ile örgüt çalışanları tarafından baskın olarak algılanan örgütsel kültür tipi arasındaki ilişkiyi incelemektir. Böylece çatışma yönetim stratejisinin belirlenmesinde, örgütsel kültür tipinin herhangi bir etkisinin olup olmadığı test edilmiş olacaktır. Çalışma, ankete dayalı bir alan araştırmasıdır ve Erzurum ilinde faaliyet gösteren bir firmada çalışan toplam 146 kişi üzerinde gerçekleştirilmiştir. Araştırma sonuçları, firma çalışanları tarafından baskın olarak algılanan örgütsel kültür tipinin pazar kültürü olduğunu ve firma yöneticileri tarafından çoğunlukla tercih edilen çatışma yönetim stratejisinin ise problem çözme olduğunu göstermiştir. İleri sürülen hipotez testi ispatlanmıştır. Buna göre yöneticiler, çatışma yönetim stratejisini belirlerken, çalışanların algıladıkları örgütsel kültür tipinden etkilenmektedirler.

    AtıfTR Dizin7
    TR Dizinİncele
  • MakaleYalnız künye2012
    Yerel yönetimlerde sosyal bütçe; Erzurum Büyükşehir Belediyesi sosyal bütçesinin değerlendirilmesi

    Kaya, Nurullah

    Yerel yönetimler, bölge halkının ortak ihtiyaçlarını karşılamak üzere kurulmuş, topluma karşı sorumlulukları olan kamu kurumlarıdır. Bu sorumluluk çerçevesinde, yerel yönetimler, halkın hizmet taleplerine cevap verebilecek kaynakları bulmak ve bu kaynakları etkin kullanmak zorundadırlar. Bunun sonucunda, yerel yönetim politikaları, belde halkının ihtiyaçları doğrultusunda şekillenmektedir. Özellikle muhtaç sosyal gruplar dikkate alınarak yerel yönetim sosyal bütçelerinin hazırlanması ve bu bütçelerin hangi ihtiyaçlar için kullanıldığına ilişkin mali tabloların, ilgili birimlere sunulması gerekmektedir. Bu çalışmada; bütçe ve sosyal politikalarla ilgili teorik bilginin yanı sıra Erzurum Büyükşehir Belediyesinin birkaç yılı kapsayan sosyal bütçesi, yıllar itibarıyla ele alınmış ve sonuçları değerlendirilmiştir.

    AtıfTR Dizin4
    TR Dizinİncele
  • MakaleYalnız künye2012
    Mûsâ cârullah'ın eğitim üzerine düşünceleri: "tarîhu'l-kur'ân ve'l-mesâhıf" adlı eseri bağlamında

    Şentürk, Mustafa

    Genel olarak bireylere kendi iradeleriyle istenen davranışların kazandırılması şeklinde tanımlanan eğitim ile ekonomik ve siyâsî ilerleme veya gerileme kavramları arasında diyalektik bir ilişki söz konusudur. Başka bir deyişle eğitim, toplumların ilerlemelerinin ya da geri kalmalarının başlangıçta sebebi sonraları ise sonucudur. Bu yaklaşıma göre 18. yy.dan itibaren Osmanlı Devleti'nin şahsında İslâm medeniyetinin en önemli gerileme nedenlerinden biri olarak eğitim sisteminin bozulması, kalkınma yolundaki ilk çare olarak da eğitim sisteminin ıslahı gösterilmiştir. Küresel ölçekli çalkantıların cereyân ettiği ve İslâm medeniyetinin yenilgisini ilan ettiği bir dönemde yaşamış olan Mûsâ Cârullah böyle düşünen mütefekkirlerdendir. Cârullah'ın eğitim ile ilgili görüşlerini serdettiği eserlerinden biri de Târîhu'l-Kur'ân ve'l-Mesâhıf'tir. Makalemiz, Cârullah'ın adı geçen eserindeki eğitim üzerine görüşlerini incelemek amacını taşımaktadır

    AtıfTR Dizin1
    TR Dizinİncele
  • MakaleYalnız künye2012
    Türkiye'de Birincil Enerji Kullanımı, Üretimi ve Gayri Safi Yurt İçi Hasıla (GSYİH) Arasındaki İlişki

    Korkmaz, Özge; Develi, Abdülkadir

    Ülkelerin sürdürülebilir bir ekonomik büyümeyi yakalayabilmeleri, öncelikle enerji kaynaklarının etkin bir şekilde kullanılmasına bağlıdır. Ancak enerji kaynaklarının dünya üzerinde eşit bir dağılım göstermemesi, Türkiye gibi yetersiz enerji kaynaklarına sahip olan ülkelerin enerjiye olan bağımlılığını arttırmaktadır. Dolayısıyla dışardan sağlanan enerji kaynaklarının iktisadi büyüme içerisindeki payının değerlendirilmesi büyük önem arz etmektedir. Bu çalışmada enerji tüketimi, enerji üretimi ile GSYİH arasındaki nedensellik ilişkisi Türkiye'nin 1960-2009 dönemi yıllık verileri kullanılarak incelenmektedir. Nedensellik sınamasında Johansen eşbütünleşme ve Granger nedensellik testleri kullanılmıştır. Johansen eşbütünleşme testi sonuçları ele alınan dönem için değişkenler arasında uzun dönemli bir ilişkinin varlığını göstermiştir. Ayrıca enerji tüketimi ile GSYİH arasında iki yönlü nedensellik tespit edilmiştir

    AtıfTR Dizin35
    TR Dizinİncele
  • MakaleYalnız künye2012
    Enerji Dönüşümü Kavramının Farklı Öğrenim Seviyelerinde Öğrenilme Durumunun Araştırılması

    Töman, Ufuk; Çimer, Sabiha Odabaşı

    Bu araştırmanın amacı; ilköğretim, ortaöğretim ve üniversite seviyelerindeöğrenim gören öğrencilerin enerji dönüşümü kavramını anlama düzeylerini vemevcut kavram yanılgılarını belirlemektir.Bu araştırmada gelişimci araştırma yöntemi kullanılmıştır. Bu yöntemdahilinde veri toplama aracı olarak kavramsal anlama testi ve yarı yapılandırılmışmülakat kullanılmıştır. Çalışmada kullanılan testte yazılı cevap gerektiren sorularayer verilmiştir. Test ilköğretimden 35, ortaöğretimden 35 ve üniversiteden 25öğrenci olmak üzere 95 öğrenciye uygulanmıştır. Ayrıca, toplam 15 öğrenciylemülakatlar yapılmıştır.Test ve mülakatlardan elde edilen veriler enerji dönüşümü kavramıyla ilgilianlamaların farklı kategorilerde olduğunu ve bu kavramın her üç öğrenimseviyesindeki öğrenciler tarafından yeterince anlaşılamadığını göstermektedir.İlköğretim seviyesindeki öğrenciler enerji dönüşümü kavramını günlük hayattakikullanımı ile ilişkilendirirken, orta öğretim ve üniversite öğrencileri iseaçıklamalarında daha çok bilimsel tanım ve okul bilgisine bağlı kalmışlardır. Ayrıca,tüm öğrenim seviyelerinde bu kavramlarla ilgili kavram yanılgıları tespit edilmiştir.

    AtıfTR Dizin4
    TR Dizinİncele
  • MakaleYalnız künye2012
    Study of bullyıng cırcumstances between 4TH and 5TH grade students of prımary educatıon accordıng to varıous varıables (rize provınce example)

    Kansu, Ceren Çevık; Kartal, Ayça; Öksüz, Yücel

    The purpose of this study is to examine the variables that could cause students to make bullying between primary education 4th and 5th grade students. The study sample consists of 294 primary school students randomly selected by sampling method ( 4-5th grade) attending primary schools located in 3 towns of Rize. This study is designed as a survey method. In the study, "Peer Bullying Survey" developed by the Pişkin (2002) has been used in order to determine the status of bully and victim. In addition, 10 students has been interviewed using 3 open-ended question developed by the researchers to be able to make further examination. Kay-square test and descriptive statistics has been used to analyze the data. The data obtained from interviews has been analyzed using the qualitative research techniques. According to findings,the vast majority of students has been implemented or exposed to bullying behaviors themselves. It has been found out that the most common type of bullying faced by the students is verbal bullying after the physical bullying, they were subjected to bullying by the bigger ones both inside and outside the school by "a male student" or "a group of male student", especially when they face with incidents of bullying, they ask for help from adults or reply to this circumstance and "physical and mental gymnastics" and "scientific awareness" category social clubs are mostly preferred by the students who make bullying

    AtıfTR Dizin5
    TR Dizinİncele
  • MakaleAçık Erişim2012
    The Qur'an Exegesis Culture Andalusia

    Sentürk, Mustafa

    this article, that we aim to examine the Qur'an exegesis culture in Andalusia, we want to handle the perception of Qur'an exegesis by searching answers for the following questions in general terms: How and when the Qur'an exegesis started in Andalusia? Who were the pioneers of the exegesis in Andalusia? What were the references of the exegesis in Andalusia? Which tafsirs were the exegesis in the region referring to? What was the methodology of exegesis in the region? Which procedure were commentators interested in among the assorted commentary drifts? What were the characteristics of interpretation in the region? Was there any difference in understanding of the Qur'an exegesis in Andalusia by comparison with other Islamic regions? If it was so, what were they? And finally, could Andalus create a school in tafsir in every respect?

    AtıfWoS1OpenAlex8
    Web of Scienceİncele
  • MakaleAçık Erişim2012
    Mediation in Islamic Jurisprudence

    Sen, Yusuf

    The conflict resolution is the most fundamental matter that is all legal systems must carry out. People who have problems between themselves find solutions to their issues through the reconcilation, the mediation, the peace/sulh, the arbitration/consolidation and the court of law. In this paper mediation is pointed out as one of the methods of the conflict resolution. Concept analysis regarding to the meditaon is being made and also the comparision between sulh/peace and other conflict resolution methods are being done. In order to provide adequate ground for the mediation, the Quran and the prophet's practices are examined. Morever, the application of the mediation in the contemprary world is discussed. The procces of the mediation and the qualifications that are asked from the mediator are explained. The paper is ended at the conclusion part with the thoughts and the considerations.

    • SDG 16 · Barış, Adalet ve Güçlü Kurumlar
    AtıfWoS2OpenAlex16
    Web of Scienceİncele
  • MakaleYalnız künye2012Scopus SJRQ4
    Expression of cytosolic and noncytosolic carbonic anhydrase enzymes from bovine brain membrane

    Demir, Nazan; Demir, Yasar; Coskun, Fatma; Tasgin, Esen

    Carbonic anhydrase is an enzyme that takes responsibility in inhalation function but, until today, carbonic anhydrase is not examined if it is present in the bovine brain membrane or not. The enzyme carbonic anhydrase was purified and separately characterized according to the bonding forms in 4 steps such as outer peripheral, cytosolic, inner peripheral and integral. Affinity chromatography was used for purification of the enzyme in all four steps. The affinity column was prepared with sepharose-4B-L-tyrosine-sulphanilamide. Purified carbonic anhydrase was obtained at each step. Enzyme activity was measured by CO2 hydratase activity and esterase activity methods. Optimum pH and optimum temperature were defined for purified enzymes at each step. Morover molecular weight and purity were detected by gel filtration and SDS-PAGE electrophorose. In addition, the enzyme's Km and nmax values were found with the Lineweaver- Burk method. The obtained results are discussed in comparison with other mammalian carbonic anhydrases. Carbonic anhydrase was shown to be exist in bovine brain membrane and this enzyme was optimized.

    AtıfScopus1
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  • MakaleYalnız künye2012Scopus SJRQ1
    Adsorption of phenol from aqueous solution on a low-cost activated carbon produced from tea industry waste: Equilibrium, kinetic, and thermodynamic study

    Gundogdu, Ali; Duran, Celal; Senturk, H. Basri; Soylak, Mustafa; Ozdes, Duygu; Serencam, Huseyin; Imamoglu, Mustafa

    The ability of activated carbon which was produced by chemical activation using zinc chloride from tea industry wastes (TIWAC) to adsorb phenol molecules from aqueous solution was tested by equilibrium, kinetic, and thermodynamic parameters. Phenol adsorption on TIWAC took place with a high yield at pH values in the range 4 to 8. The optimum contact period was observed as 4.0 h and from the adsorption graphs plotted as a function of time; it was established that phenol adsorption on TIWAC conformed more to a pseudosecond-order kinetic model. Additionally, it was determined that the adsorption rate is controlled by intraparticle diffusion as well as film diffusion. It was established that phenol adsorption on TIWAC can be better defined by the Langmuir adsorption model and its adsorption capacity was 142.9 mg·g-1 from the linear Langmuir equation. Temperature had an adverse effect on adsorption yield, and hence, the adsorption process was exothermic in our case. Moreover, increasing electrolyte concentration in the medium has a positive effect on adsorption yield. From the data obtained, it was concluded that the removal of phenol from aqueous solution by TIWAC produced from tea industry wastes with a very low cost took place with an extremely high performance.

    • SDG 6 · Temiz Su ve Sanitasyon
    • SDG 12 · Sorumlu Üretim ve Tüketim
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