İlgi alanları

• MakaleYalnız künye2026/r/bayburt/11440
  Microwave-assisted extraction of pectin from persimmon: physicochemical properties, structural characterization and greenness assessment

  Yılmaz, Özlem; Kutlu, Naciye; Kamiloğlu, Aybike; Elbir Abca, Tuğba

  In this study, the microwave-assisted extraction (MAE) of pectin from persimmon was optimized using a Box-Behnken experimental design, with pH, extraction time, and liquid-to-solid ratio as independent variables. The optimization results obtained through response surface methodology indicated optimum conditions of pH 1.78, extraction time of 8 min, and a liquid-to-solid ratio of 125 mL/g, corresponding to a predicted pectin yield of 4.28%. In the validation experiment, the actual pectin yield was determined to be 3.79 ± 0.04%, confirming a good agreement with the model predictions. The degree of esterification, methoxyl content, galacturonic acid and ash contents, water- and oil-holding capacities, antioxidant activity, rheological behavior, and structural properties (FT-IR, DSC, and 1H NMR) of the samples obtained under optimal MAE and conventional extraction conditions were compared. Based on the degree of esterification, both samples were classified as high-methoxyl, with galacturonic acid contents of 41% and 42% for the MAE and conventionally extracted samples, respectively; both values were below the ≥ 65% level specified for commercial food-grade pectin. The ash content was higher in the MAE sample (7.22%) than in the conventionally extracted sample (1.56%). MAE resulted in a pectin-rich complex with a relatively low galacturonic acid content and higher antioxidant activity than the conventionally extracted sample. Furthermore, the MAE sample exhibited higher water-holding capacity but lower oil-binding capacity than the conventionally extracted sample. Rheological analysis showed that the MAE sample exhibited higher apparent viscosity and shear stress than the conventionally extracted sample. FT-IR analysis revealed more pronounced absorption bands in the MAE sample, suggesting structural differences. DSC analysis revealed differences in the thermal behavior of the MAE and conventionally extracted samples. Additionally, 1H NMR analysis supported the presence of characteristic pectic structural features in both samples. The greenness assessment of the method was assessed using the Greenness Evaluation Metric for Analytical Methods (GEMAM), which yielded an overall score of 7.482, indicating a high level of environmental acceptability. Overall, MAE can be considered an efficient and sustainable approach for obtaining a pectin-rich complex with antioxidant activity from persimmon.

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• MakaleYalnız künye2026/r/bayburt/11329
  Optimization of Antioxidant Recovery from Deep Eutectic Solvent Phase via Back-Extraction Using Taguchi Method

  Kamiloğlu, Aybike; Elbir Abca, Tuğba; Kutlu, Naciye

  This study investigates the use of deep eutectic solvents (DESs) via ethyl acetate-mediated liquid–liquid extraction and the optimization of the process using the Taguchi method for the recovery of antioxidant compounds from tea industry waste. In the study, choline chloride was used as a hydrogen bond acceptor; lactic acid, glycerol, and ethanol were used as hydrogen bond donors; and different ratios of water (10–30%) were added to reduce the viscosity of the DESs. Ultrasound-assisted extraction results showed that lactic acid-based DESs provided the highest total phenolic content and antioxidant activity due to their ability to break down plant cell walls. However, in the recovery phase carried out with ethyl acetate, it was determined that ethanol-based DESs (ChCl:EtOH) were more effective in transferring antioxidants to the organic phase due to their looser molecular structure. Optimization using the Taguchi L9 orthogonal array revealed the following results: ChCl:EtOH solvent, 2:1 ethyl acetate/extract ratio, 20% water ratio, and 30-min shaking period were determined as the optimal conditions. Under these conditions, the model predicted a yield of 93.34%, and experimental validation revealed a recovery yield of 85.70 ± 1.13%. The results demonstrate that this developed green extraction method offers a sustainable alternative for converting tea waste into high value-added functional components.

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• MakaleYayıncıda açık erişim2026/r/bayburt/11303
  Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Polyphenols From Tea Parts: A Green Assessment

  Gerçek, Yusuf Can; Baştürk, Fatma Nur; Gıdık, Betül; Abca, Tuğba Elbir; Yurtvermez, Bayram; Fuente-Ballesteros, Adrián; Kutlu, Naciye; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have emerged as green alternatives to conventional solvents for extracting bioactive compounds from plants. This study evaluated nine choline chloride–based DESs for recovering antioxidant polyphenols from four parts of the tea plant (Camellia sinensis): seed, bud, leaf, and flower. Total phenolic content, antioxidant activity, and individual polyphenolic profiles were determined, and the greenness of the sample preparation was assessed using AGREEprep. The highest total phenolic content (53.28 mg GAE/g) and antioxidant activity (141.17 mg TE/g) were obtained from leaves using ChCl:formic acid, outperforming ethanol and methanol. Leaves also exhibited the highest total quantified polyphenolic content (up to 95,813 mg/kg DW, corresponding to the sum of the quantified polyphenols), with epicatechin gallate and gallocatechin + epigallocatechin being the major contributors. Flowers and buds showed notable polyphenolic diversity, while seeds contained the lowest levels. Statistical analysis confirmed clear clustering patterns according to plant part and solvent type. AGREEprep evaluation yielded scores up to 0.54, highlighting the environmental benefits of DES-based extraction. We demonstrated that tea leaves were the richest source of bioactive polyphenols, and selected DESs, particularly formic acid–based and ethylene glycol–based, exhibited superior efficiency and sustainability compared to conventional solvents. These findings support the wider adoption of DESs for green, high-yield extraction of pharmacologically important compounds from tea and other medicinal plants.

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• MakaleYalnız künye2026/r/bayburt/11268
  Optimizing microwave drying parameters for rhododendron bee pollen: effects on antioxidant potential, polyphenols, and proteins

  Kutlu, Naciye; Gercek, Yusuf Can; Ecem Bayram, Nesrin

  Monofloral Rhododendron spp. bee pollen is a remarkable bee product due to its functional components as well as its potential pharmacological effects. In this study, Rhododendron spp. bee pollen was dried by the microwave method as an alternative to the conventional drying method. Different combinations of microwave powers (140, 210 and 280 W) and sample weights (10, 20 and 30 g) applied during microwave drying (MWD) were determined by Central Composite Design. Drying time, total protein, total carotenoid, total phenolic-flavonoid compounds, and proanthocyanidin yields of dried pollen were determined and antioxidant activity tests (CUPRAC and DPPH•) were performed. The analysis results were optimized by response surface methodology (RSM) and the optimum drying conditions were obtained when the microwave power was 280 W and the sample weight was 30 g. The optimal conditions have been established to reduce drying time and enhance the content of bioactive compounds. As a result of optimization, the composite desirability (d) was found to be 0.8. Experimental and predicted values were close to each other. In conclusion, MWD has demonstrated efficacy as a drying technique by significantly reducing drying time while maintaining and enhancing the yields of bioactive compounds in monofloral Rhododendron spp. bee pollen. In comparison to conventional techniques, the application of MWD in the drying process of monofloral Rhododendron spp. bee pollen is demonstrably more efficient and sustainable.

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• MakaleYayıncıda açık erişim2026/r/bayburt/11039
  Deep Eutectic Solvents as Sustainable Media for Efficient Recovery of Phenolics From Black (Lycium ruthenicum Murr.) and Red (Lycium barbarum Lam.) Goji Berries: Optimization and Functional Evaluation

  Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Çevik, Berke; Baştürk, Fatma Nur; Özmener, Bahar; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have been frequently preferred over the past decade as alternatives to conventional solvents for the extraction of bioactive compounds from natural products. Meanwhile, black and red goji berries are widely consumed as functional foods due to their many functional properties reported in the literature. The aim of this study is to evaluate the effectiveness of different DESs in the extraction of individual phenolic compounds from goji berry (Lycium barbarum and L. ruthenicum) fruits, to determine the most suitable DES, and to optimize the extraction conditions. The DES that provided the highest yield in the extraction of individual phenolic compounds from L. barbarum was determined to be proline:citric acid (DES6 – a total of approximately 33,545 µg/kg), while for L. ruthenicum it was choline chloride:acetic acid (DES3 – a total of approximately 37,496 µg/kg). Rutin was the predominant phenolic compound in both species. DESs provided a higher phenolic yield compared to ethanol and water. In addition, L. ruthenicum exhibited higher total phenolics, flavonoids, proanthocyanidins, and antioxidant activity compared to L. barbarum. These findings reveal that DESs are a green, efficient, and sustainable alternative for the extraction of phenolic compounds from goji berries.

  AtıfScopus1·WoS1

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• MakaleYalnız künye2025/r/bayburt/12645
  Optimized ultrasonic-NaDES extraction of anthocyanins, polyphenolics, and organic acids from chokeberry fruit with blueness and antimicrobial evaluation

  Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Kırkıncı, Seran; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have been widely used in food processing in recent years, owing to their remarkable advantages. The aim of this study was to evaluate the potential of DESs for the recovery of bioactive components from chokeberry fruit. First, among the 10 different DESs, the DES that extracted the highest total individual phenolic content (TIPC) from chokeberry was determined. Then, ultrasonically assisted extraction conditions (ultrasonic amplitude, time, and temperature) were optimized by response surface methodology to obtain the maximum amount of TIPC using this DES. According to the results obtained, the natural deep eutectic solvent (NaDES) choline chloride: acetic acid (1:2, 30% water content) provided the highest yield for TIPC extraction and the optimum conditions for maximum TIPC extraction were determined as 100% for ultrasonic amplitude, 50 °C for temperature and 60 min for sonication time. The TIPC content of the extracts prepared with this NaDES was determined between 1740.26 and 3703.14 mg/kg. In addition, the individual organic acid and individual anthocyanin contents of the NaDES extracts, as well as their antioxidant and antimicrobial activities, were determined. Chlorogenic acid (1392.351–2361.48 mg/kg) among phenolic compounds, pyruvic acid (9361.35–47398.04 mg/kg) among organic acids and kuromanin chloride (69748.13–134668.24 mg/kg) among anthocyanins were the dominant compounds in NaDES extracts. However, the extracts showed varying levels of antimicrobial activity against Gram (+) bacteria, Gram (−) bacteria, and yeast-like fungi (Candida strains). Finally, the Blue Applicability Grade Index (BAGI) was used to evaluate the practicality/blueness potential of the chromatographic method used to determine the anthocyanin composition of the DES extracts. In conclusion, NaDES (choline chloride:acetic acid) can be an alternative solvent for the recovery of some individual phenolics, organic acids and anthocyanins under different extraction conditions. In addition, the use of NaDES in the recovery of various functional compounds needed in food, cosmetics, pharmaceuticals, etc., is important for the promotion of sustainability and green technology.

  AtıfScopus19·WoS18

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• MakaleYayıncıda açık erişim2025/r/bayburt/11815
  Optimization of Hemp Seed (Cannabis sativa L.) Protein Extraction and Glycation Conditions by Response Surface Methodology: Physicochemical, Functional, and Thermal Characteristics

  Yüzer, Elvan; Kutlu, Naciye; Yüzer, Mustafa Onur

  In this study, first, optimization using the response surface method was implemented to obtain the highest yield of protein isolate from defatted hemp seed under different extraction conditions. Optimum extraction conditions were determined with 0% salt concentration, 12 pH value, and 49°C temperature. The protein isolate produced under optimum conditions was subjected to the glycation process, and the glycation conditions were also optimized. The optimum conditions for glycation were determined as a protein isolate: sugar ratio of 1.6:1, temperature of 60°C, and time of 90 min, providing the most suitable glycation degrees and browning indices. There was no statistical difference between the glycated protein isolate obtained under these conditions and the nonglycated protein isolate in terms of emulsifying activity index and emulsion stability index. For total phenolic–flavonoid compounds and antioxidant activity, the sugar complex caused a decrease in these values. In addition, there was a high positive correlation between bioactive compounds. SEM micrographs showed relationships between hemp seed protein isolate and sugar, and these interactions led to a spongy three-dimensional microstructure. When the FTIR results were analyzed, the glycated isolate had stronger absorption at 3300–3200 cm−1 compared to the nonglycated isolate. In conclusion, this study reports important findings in terms of functional and characteristic properties of how monosaccharides affect glycation.

  AtıfScopus1·WoS1

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• MakaleYalnız künye2025/r/bayburt/11574
  The impact of osmotic dehydration and microwave drying process conditions on the quality characteristics of celery roots (Apium graveolens l. Subsp. rapaceum)

  Kutlu, Naciye

  In this study, celery roots were dried by the osmotic dehydration-assisted microwave drying method, and both osmotic dehydration (OD) and microwave conditions were optimized using response surface methodology (RSM). Utilizing a Box–Behnken experimental design, the drying time was minimized while maximizing the total phenolic compounds (TPC) in dried samples at different OD salt concentrations (10, 20 and 30 %), OD temperatures (30, 45 and 60 °C), OD durations (1, 2 and 3 h) and microwave powers (210, 350 and 490 W). The optimal drying conditions were identified as 15.05 % for OD salt concentration, 42.5 °C for OD temperature, 1.6 h for OD duration and 489.7 W for microwave power. Under these optimal conditions, the predicted values for time and TPC were 10 min and 104.38 mg GAE/100 g, respectively. The composite desirability value was 0.99 as a result of optimization. The experimental values for time and TPC were measured as 11 min and 97.45 mg GAE/100 g, respectively. The predicted and experimental values were found to be statistically similar to each other. A comparison with control groups designed to assess the effects of OD and microwave processes revealed that these processes significantly influenced both quality (TPC, antioxidant activity, color) and drying characteristics (drying time, rehydration rate). The greenness assessment value was calculated as 78 using the MoGAPI tool. Consequently, the application of OD pretreatment and use of the innovative microwave drying method as a final drying step in vegetable drying was successfully implemented to enhance the properties of the final product.

  AtıfScopus3·WoS3

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• MakaleYalnız künye2025/r/bayburt/11142
  Extraction of Bioactive Compounds from Celery Root Peels Using an Ultrasound-Assisted Method with Choline Chloride–Based Deep Eutectic Solvents

  Kamiloğlu, Aybike; Kutlu, Naciye; Elbir Abca, Tuğba; Yılmaz, Özlem

  The concepts of sustainable and green technology have come to the forefront due to problems such as increasing environmental pollution, depletion of resources, and uncontrolled increases in industrialization. Green chemistry aims to reduce or completely eliminate pollution in the environment. In this study, both the ultrasonic method, which is a form of green technology, and deep eutectic solvents, which are among green solvents, were used, and a perspective on creating economic value from a waste product (celery root peel) is presented. In this context, phytochemical compounds (total flavonoid content and antioxidant activities) were obtained from celery root peels by ultrasonic-assisted extraction using 10 different choline chloride–based deep eutectic solvents, and the results were compared with methanol, an organic solvent. As a result, according to the total flavonoid content, the most effective deep eutectic solvent was choline chloride to sucrose, while according to the antioxidant activity value, choline chloride to lactic acid was found to be effective. For all of the analyzed bioactive compounds, higher or equal yields were obtained with all deep eutectic solvent extracts compared to methanol, and deep eutectic solvents could be alternatives to organic solvents (such as methanol) that may have toxic effects on humans or the environment.

  AtıfScopus9·WoS9

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• MakaleYalnız künye2025/r/bayburt/11119
  Influence of ultrasonic-assisted deep eutectic solvent extraction on bioactive compounds from germinated mung beans (Vigna radiata) and greenness assessment

  Yücel, Tuğçenur; Kamiloğlu, Aybike; Kutlu, Naciye

  In this study, the ultrasonic-assisted method was applied to extract phenolic, flavonoid, and antioxidant compounds from germinated mung beans. In addition, deep eutectic solvents (DESs) were chosen as solvents to support the green concept and to increase their applicability. Choline chloride is a quaternary ammonium salt and was used as the hydrogen bond acceptor (HBA), and compounds from the alcohol, organic acid, sugar, and amide groups were combined as hydrogen bond donors (HBD) to obtain 8 different DESs. Higher yields were obtained with ultrasonic-assisted DESs compared to the traditional method (maceration) when total phenolic compound and antioxidant activities were evaluated. While the most effective solvent for total phenolic compound yield was choline chloride:fructose, the highest antioxidant activity values were obtained with solvents using organic acids as HBD. Moreover, Blue Applicability Grade Index (BAGI) was used to assess the greenness of the applied ultrasonic-assisted spectrophotometric extraction and DESs. As a result, using green technology and green solvents was influential in the extraction of bioactive compounds, and their applicability was especially effective for mung beans.

  AtıfScopus8·WoS7

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