İlgi alanları

• MakaleYalnız künye2026/r/bayburt/12682
  Profile of honey, bee pollen and bee bread: comparative shotgun sequencing

  Morgil, Hande; Ecem Bayram, Nesrin

  The plant origin of the bee products often influences their value. Here, we investigated for the first time how the content of bee bread, a functional food with high nutritional value, differs in terms of plant and bacterial taxa in comparison with bee pollen and honey samples, using shotgun sequencing analysis. Sequencing of honey, bee pollen, and bee bread samples yielded approximately 32.74 Gbp of data. Out of a total of 268,964 abundant genes, 70,280 genes (26.13%) were assigned to a taxonomic group according to the supermajority rule. These taxonomic groups were annotated as follows: Kingdom (84.99%), phylum (75.94%), class (73.26%), order (67.21%), and family (65.11%). It was determined that honey, bee pollen, and bee bread samples consisted of large plant families Poaceae, Fabaceae and Asteraceae. This indicates that these taxa are also favored by bees for visiting, and are therefore used as food sources. These differences vary according to the environmental flora of the apiary and may be site specific. Based on these results, DNA-based next-generation sequencing techniques can be successfully used to identify the plant sources of bee products. Thus, the botanical and geographical origin of bee product samples can be more accurately, reliably and in detail identified. Furthermore, this technique can be successfully used to monitor the regional flora and food safety.

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• MakaleYayıncıda açık erişim2026/r/bayburt/12663
  Anatomical and Phytochemical Characterization of Ranunculus pinardii and Ranunculus constantinopolitanus: LC-MS/MS-Based Bioactive Compound Profiling

  Feizollahi, Sepideh; Gercek, Yusuf Can; Canlı, Deniz; Basturk, Fatma Nur; Yesil, Yeter; Kösesakal, Taylan; Ecem Bayram, Nesrin

  The aim of this study was to characterize Ranunculus pinardii and Ranunculus constantinopolitanus species anatomically and phytochemically. In order to contribute to the taxonomic characterization of R. pinardii and R. constantinopolitanus, anatomical structures of the stem, leaf, and petiole of species were examined with light microscopy. Total phenolic-flavonoid content, antioxidant activity, total anthocyanin, total proanthocyanidin, and individual phenolic compounds of extracts prepared with different solvents were determined. The results showed that the total phenolic contents of the species had a similar range (0.09-0.45 mg GAE/g for R. pinardii and 0.01-0.44 mg GAE/g for R. constantinopolitanus), but R. constantinopolitanus exhibited higher antioxidant activity (29.28 mg TE/g). Higher individual phenolic compound recovery was achieved from R. pinardii compared to R. constantinopolitanus with all solvents, except methanol. Both species were rich in luteolin, a natural flavonoid (163.98-34417.11 µg/kg for R. pinardii and 44.93-129009.44 µg/kg for R. constantinopolitanus). These results indicate that these plants contain bioactive ingredients that may have applications in different fields, such as medicine, cosmetics, and food. In particular, the detection of luteolin compound at high levels in these species compared to other components indicates that optimization studies aimed at the recovery of luteolin are important for future research.

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• MakaleYayıncıda açık erişim2026/r/bayburt/11303
  Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Polyphenols From Tea Parts: A Green Assessment

  Gerçek, Yusuf Can; Baştürk, Fatma Nur; Gıdık, Betül; Abca, Tuğba Elbir; Yurtvermez, Bayram; Fuente-Ballesteros, Adrián; Kutlu, Naciye; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have emerged as green alternatives to conventional solvents for extracting bioactive compounds from plants. This study evaluated nine choline chloride–based DESs for recovering antioxidant polyphenols from four parts of the tea plant (Camellia sinensis): seed, bud, leaf, and flower. Total phenolic content, antioxidant activity, and individual polyphenolic profiles were determined, and the greenness of the sample preparation was assessed using AGREEprep. The highest total phenolic content (53.28 mg GAE/g) and antioxidant activity (141.17 mg TE/g) were obtained from leaves using ChCl:formic acid, outperforming ethanol and methanol. Leaves also exhibited the highest total quantified polyphenolic content (up to 95,813 mg/kg DW, corresponding to the sum of the quantified polyphenols), with epicatechin gallate and gallocatechin + epigallocatechin being the major contributors. Flowers and buds showed notable polyphenolic diversity, while seeds contained the lowest levels. Statistical analysis confirmed clear clustering patterns according to plant part and solvent type. AGREEprep evaluation yielded scores up to 0.54, highlighting the environmental benefits of DES-based extraction. We demonstrated that tea leaves were the richest source of bioactive polyphenols, and selected DESs, particularly formic acid–based and ethylene glycol–based, exhibited superior efficiency and sustainability compared to conventional solvents. These findings support the wider adoption of DESs for green, high-yield extraction of pharmacologically important compounds from tea and other medicinal plants.

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• MakaleYalnız künye2026/r/bayburt/11268
  Optimizing microwave drying parameters for rhododendron bee pollen: effects on antioxidant potential, polyphenols, and proteins

  Kutlu, Naciye; Gercek, Yusuf Can; Ecem Bayram, Nesrin

  Monofloral Rhododendron spp. bee pollen is a remarkable bee product due to its functional components as well as its potential pharmacological effects. In this study, Rhododendron spp. bee pollen was dried by the microwave method as an alternative to the conventional drying method. Different combinations of microwave powers (140, 210 and 280 W) and sample weights (10, 20 and 30 g) applied during microwave drying (MWD) were determined by Central Composite Design. Drying time, total protein, total carotenoid, total phenolic-flavonoid compounds, and proanthocyanidin yields of dried pollen were determined and antioxidant activity tests (CUPRAC and DPPH•) were performed. The analysis results were optimized by response surface methodology (RSM) and the optimum drying conditions were obtained when the microwave power was 280 W and the sample weight was 30 g. The optimal conditions have been established to reduce drying time and enhance the content of bioactive compounds. As a result of optimization, the composite desirability (d) was found to be 0.8. Experimental and predicted values were close to each other. In conclusion, MWD has demonstrated efficacy as a drying technique by significantly reducing drying time while maintaining and enhancing the yields of bioactive compounds in monofloral Rhododendron spp. bee pollen. In comparison to conventional techniques, the application of MWD in the drying process of monofloral Rhododendron spp. bee pollen is demonstrably more efficient and sustainable.

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• MakaleYayıncıda açık erişim2026/r/bayburt/11039
  Deep Eutectic Solvents as Sustainable Media for Efficient Recovery of Phenolics From Black (Lycium ruthenicum Murr.) and Red (Lycium barbarum Lam.) Goji Berries: Optimization and Functional Evaluation

  Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Çevik, Berke; Baştürk, Fatma Nur; Özmener, Bahar; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have been frequently preferred over the past decade as alternatives to conventional solvents for the extraction of bioactive compounds from natural products. Meanwhile, black and red goji berries are widely consumed as functional foods due to their many functional properties reported in the literature. The aim of this study is to evaluate the effectiveness of different DESs in the extraction of individual phenolic compounds from goji berry (Lycium barbarum and L. ruthenicum) fruits, to determine the most suitable DES, and to optimize the extraction conditions. The DES that provided the highest yield in the extraction of individual phenolic compounds from L. barbarum was determined to be proline:citric acid (DES6 – a total of approximately 33,545 µg/kg), while for L. ruthenicum it was choline chloride:acetic acid (DES3 – a total of approximately 37,496 µg/kg). Rutin was the predominant phenolic compound in both species. DESs provided a higher phenolic yield compared to ethanol and water. In addition, L. ruthenicum exhibited higher total phenolics, flavonoids, proanthocyanidins, and antioxidant activity compared to L. barbarum. These findings reveal that DESs are a green, efficient, and sustainable alternative for the extraction of phenolic compounds from goji berries.

  AtıfScopus1·WoS1

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• MakaleYayıncıda açık erişim2025/r/bayburt/12768
  Sustainable Optimization of Ohmic Heating–Assisted Extraction of Polyphenolics from Goji Berry (Lycium barbarum L.): LC–MS/MS Profiling and Blueness–Greenness Assessment

  Kantar, Harun; Ecem Bayram, Nesrin

  This study aimed to extract bioactive compounds from goji berry (Lycium barbarum L.) fruit using the ohmic heating–assisted extraction (OHAE) method and optimize the extraction conditions. In this context, firstly, the extraction conditions to be applied for the OHAE method were determined using the response surface method (RSM)-Box Behnken experimental design. During the study, temperature, holding time, and electrical field strength were determined as independent variables, while the total phenolic compound (TPC), total flavonoid compound (TFC), and antioxidant activity (DPPH) were selected as dependent variables. As a result of the optimization, the optimum conditions were determined to be 60 °C for the temperature, 60 min for the holding time, and 45 V/cm for the electrical field strength, and the composite desirability value of the model was 0.90. The highest experimental results for TPC, TFC, and DPPH were found to be 23.83 mg GAE/g, 7.33 mg QE/g, and 24.53 mg TE/g, respectively. In addition, the polyphenolic profiles of all extracts obtained under different process conditions were determined by LC–MS/MS, and rutin was the phenolic detected at the highest rate in the extracts. In addition, the blueness and greenness of the analytical method used in this study were evaluated using the BAGI and ComplexGAPI metrics, respectively. The results indicate that OHAE is an effective and sustainable method for extracting bioactive compounds from goji berries.

  AtıfScopus5·WoS5

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• MakaleYalnız künye2025/r/bayburt/12645
  Optimized ultrasonic-NaDES extraction of anthocyanins, polyphenolics, and organic acids from chokeberry fruit with blueness and antimicrobial evaluation

  Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Kırkıncı, Seran; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) have been widely used in food processing in recent years, owing to their remarkable advantages. The aim of this study was to evaluate the potential of DESs for the recovery of bioactive components from chokeberry fruit. First, among the 10 different DESs, the DES that extracted the highest total individual phenolic content (TIPC) from chokeberry was determined. Then, ultrasonically assisted extraction conditions (ultrasonic amplitude, time, and temperature) were optimized by response surface methodology to obtain the maximum amount of TIPC using this DES. According to the results obtained, the natural deep eutectic solvent (NaDES) choline chloride: acetic acid (1:2, 30% water content) provided the highest yield for TIPC extraction and the optimum conditions for maximum TIPC extraction were determined as 100% for ultrasonic amplitude, 50 °C for temperature and 60 min for sonication time. The TIPC content of the extracts prepared with this NaDES was determined between 1740.26 and 3703.14 mg/kg. In addition, the individual organic acid and individual anthocyanin contents of the NaDES extracts, as well as their antioxidant and antimicrobial activities, were determined. Chlorogenic acid (1392.351–2361.48 mg/kg) among phenolic compounds, pyruvic acid (9361.35–47398.04 mg/kg) among organic acids and kuromanin chloride (69748.13–134668.24 mg/kg) among anthocyanins were the dominant compounds in NaDES extracts. However, the extracts showed varying levels of antimicrobial activity against Gram (+) bacteria, Gram (−) bacteria, and yeast-like fungi (Candida strains). Finally, the Blue Applicability Grade Index (BAGI) was used to evaluate the practicality/blueness potential of the chromatographic method used to determine the anthocyanin composition of the DES extracts. In conclusion, NaDES (choline chloride:acetic acid) can be an alternative solvent for the recovery of some individual phenolics, organic acids and anthocyanins under different extraction conditions. In addition, the use of NaDES in the recovery of various functional compounds needed in food, cosmetics, pharmaceuticals, etc., is important for the promotion of sustainability and green technology.

  AtıfScopus19·WoS18

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• MakaleYalnız künye2025/r/bayburt/11394
  Quantification of phenolics by LC-MS/MS of monofloral and polyfloral Honey: Evaluation of the greenness of the method with MoGAPI and MoGSA

  Gerçek, Yusuf Can; Baştürk, Fatma Nur; Özkök, Aslı; Cevahir Öz, Gül; Ecem Bayram, Nesrin

  This study evaluated the physicochemical properties, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, proanthocyanidin content, and polyphenolic profiles of monofloral and multifloral honey samples produced in Türkiye. In addition, the greenness of the analytical method was assessed using two different evaluation tools: MoGSA and MoGAPI. The TPC and TFC values for multifloral honeys ranged from 0.18–0.65 mg GAE/g and 0.01–0.09 mg QE/g, respectively, whereas for monofloral honeys, these values ranged from 0.16–0.98 mg GAE/g and 0.01–0.37 mg QE/g. The highest CUPRAC and DPPH values were observed in Erica spp. honey, consistent with its TFC and total individual polyphenolic compound results. Notably, the Erica spp. honey sample exhibited a significantly higher gallic acid content (26.13 µg/g) compared to the other samples. These findings suggest that Erica spp. honey characterized by its high TPC, antioxidant activity, and distinctive polyphenolic profile, may serve as a notable dietary source of antioxidants. Additionally, the MoGAPI and MoGSA scores of the method were determined to be 84 and 91.67, respectively, demonstrating its high environmental sustainability.

  AtıfScopus4·WoS4

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• MakaleYayıncıda açık erişim2025/r/bayburt/11130
  Extraction of Phenolic Compounds From Lavender (Lavandula angustifolia L.) Plant by Natural Deep Eutectic Solvent and Greenness Assessment of Analytical Method With Modified Green Analytical Procedure Index

  Ecem Bayram, Nesrin; Yenigün, Baran; Gerçek, Yusuf Can

  In recent years, it has been important in terms of green technology and sustainability to promote the use of natural deep eutectic solvents (NADESs), especially those based on natural products, for the extraction of bioactive components with different functional properties from foods. Therefore, in this study, the effects of green extraction technology (ultrasonic-assisted extraction) and green solvents (NADESs) on the recovery of bioactive components from lavender (Lavandula angustifolia L.) were investigated and evaluated. In addition, the degree of greenness of the analytical method used during the study was determined using the modified green analytical procedure index (MoGAPI). In this context, the total phenolic content (TPC), total antioxidant capacity, and individual polyphenolic profiles of lavender extracts prepared with two different NADES were investigated by liquid chromatography-tandem mass spectrophotometer. Based on the results obtained, it was determined that NADES formed with choline chloride:formic acid (ChCl:FA) had a higher potential to extract bioactive components from lavender than NADES formed with ChCl:ethylene glycol (ChCl:EG). The TPC, antioxidant capacity, and total individual polyphenolic compound content of lavender extracted with these NADES were determined as 9.76 mg gallic acid equivalent/g, 20.83–104.39 mg Trolox equivalent/g, and 200.835 mg/kg, respectively. However, the degree of greenness determined by the MoGAPI tool of the analytical method used in this study was 78% and classified as ‘excellent green’. As a result, it can be concluded that NADES formed with ChCl:FA can be an alternative to conventional solvents for the extraction of bioactive components from lavender, as it provides a remarkable recovery of bioactive substances.

  AtıfScopus5·WoS4

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• Kitap BölümüYayıncıda açık erişim2024/r/bayburt/13140
  Pollen Morphology and Anatomy with Botanical Preferences Made by Bees: An Introduction Data

  Canlı, Deniz; Bayram, Nesrin Ecem

  Pollen is a microspore containing vegetative and generative cells prepared for pollination in seed plants. Because pollen grains show highly variable characteristics, these structures are used to evaluate kinship relationships and similarity levels in plants. However, plant sources of honey, which is consumed as an important food source and functional food, can also be determined by identifying the pollen grains included in the honey by bees. In this study, the characteristic parameters used for the identification of pollen grains in plants and the plant sources frequently visited by bees were mentioned. As a result, variables such as size, polarity and symmetry, shape, exine sculpturing, intine properties and apertures are the main characteristics used to describe pollen grains. Considering these variables, it can contribute to the identification of plant taxa. In addition, the plants that honey bees visit to collect nectar and the botanical origin/source of honey (monofloral-multifloral) can also be determined by identifying the pollen grains included in the honey.

  AtıfScopus5

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