Sürdürülebilir Kalkınma Amaçları

Yazarlar

  • Derleme MakaleYalnız künye2018
    Poet Faruk Shousha “Yakulu ed-demu al-Arabi Called” -A Structuralist Approach-

    İbrahım Mohamed İbrahım, Dessoukı

    In this study, Dewan ( the Arab blood says) by the poet Farouk Shousha was analysed with structuralist method used in modern poetry analyses. The researcher thinks that the approach of structuralist criticism is one of the most convenient methods in literary textual analysis.

  • Derleme MakaleYalnız künye2018
    Hz. Peygamber’in Gayri Müşriklerle Olan İlişkisi

    Abdelaziz, Eid Fathi Abdellatif; Topkarci, Mehmet Akin

    Bu araştırma Hz. Peygamber’in Mekke-i Mükerreme ve ArapYarımadası’nda yaşayan müşriklerle olan ilişkilerini siret-i nebi,sünnet ve Kuran-ı Kerim çerçevesinde inceler.

  • MakaleYalnız künye2018
    Word familiarity effects in efl reading: an eye tracking study

    Dolgunsöz, Emrah

    Do you know what happens in mind when we encounter a novel word while reading anewspaper, a paragraph or a short story? Via eye tracking technique, this study aimed togather clues about how our mind reacts to an unknown word while we read in anotherlanguage by examining word familiarity effects on eye movements during EFL reading. Aftera vocabulary test, eye movements of 60 EFL learners at intermediate level were recordedwhile they read identical sentence pairs including words with different length and frequency.The results showed that (1) total time spent on words and reanalysis times were predicted byvocabulary knowledge, (2) initial word processing was not predicted by vocabulary size (3)unfamiliar words attended more attention and required more cognitive effort than familiarwords, (4) familiar words are processed in similar time spans for English as L1 and L2 butunfamiliar words cause more cognitive load in EFL. The results were discussed incomparison to L1 eye movement research in reading and were evaluated regarding EFLreading in terms of noticing hypothesis, incidental vocabulary acquisition and EFL readinginstruction.

  • MakaleYalnız künye2018
    Teaching efl vocabulary to young digital nativesthrough online games: a study with turkish 5TH grade efl learners

    Dolgunsöz, Emrah; Bilici, Özlem Utku

    The current study aimed to examine the effect of online vocabulary gameson teaching new words to young learners ofEFL. For this aim, 46 Turkish 5thgrade EFL students in a state school were assigned into control and experimental groups. Both groups studied 22 words in 6 instructional sessions conducted in 6 subsequent weeks in the second semester of 2016-2017academicyear. Throughout this period, the data were collected via recognition-production tests and semi-structured interviews. The results of the recognition and production tests showed that experimental group outperformed the control group in terms of vocabulary gains. The results of the semi-structured interviews also supported the quantitativeresultsindicatingthatonlinevocabularygamesincreased students’ motivation. Additionally, it was observedthat young learners were amused by digital games and were willing to learn more via them.

  • MakaleYalnız künye2018
    Türk Siyasal Hayatında Asker-Sivil İlişkileri, 15 Temmuz ve Dinî Gruplar Meselesi

    Peçe, Halil; Bilici, İlhan

    Türk siyasal hayatında asker-sivil ilişkileri her dönem önem arz etmiştir. Ordubelirli periyotlarla sivil yönetimi “yola getirme” noktasında inisiyatif alıpsiyasete müdahil olmuş ve bunun neticesinde darbeler meydana gelmiştir. Herne kadar bu darbeler her zaman geleneksel askeri metotlar çerçevesindegerçekleşmese de askeri elitler, istenen sonuca ulaşma bağlamında etkiliolmuşlardır. Asker-sivil ilişkilerinde önemli en son gelişme 15 Temmuz 2016tarihinde ordu içine yuvalanmış FETÖ örgütü eliyle gerçekleşen darbegirişimidir. Bu darbe girişimi neticesinde asker-sivil ilişkilerine dairtartışmaların yeniden hararet kazanması yanında, dinî gruplar meselesi farklıboyutlarıyla ele alınmaya başlamıştır. Bu çalışma Türk siyasal hayatındakiasker-sivil ilişkilerine değinmenin yanında, 15 Temmuz darbe girişiminin dinîgruplar boyutuyla nasıl ele alınması gerektiğine dair bir tartışmaya kapıaçmaktadır.

    AtıfTR Dizin1
    TR Dizinİncele
  • MakaleYalnız künye2018
    HISTORY EDUCATION and TEACHING in "RESIMLI TARIH MECMUASI" WHICH is the 1950s POPULAR HISTORY MAGAZINE

    Elban, Mehmet

    The objective of this research study is to examine the history education and teaching in the popular history magazine of 1950s, "Resimli Tarih Mecmuası” (lit. Illustrated History Magazine). In the study, documentary research technique was used, which is among qualitative research methods. The research universe of the study is comprised of 23 issues of this magazine. Content analysis and descriptive analysis were used in the analysis of the data obtained in the study. One of the important results that the study revealed was that, history, according to the magazine, is to understand the past and present. History is an entertaining and educating means. Besides, the magazine defines history as the activities of the great men. Since the magazine took into consideration the letters from the readers, it is understood that the popular history content of the magazine was determined by its audience. In the narration of the male heroes in the magazine, the emphasis is on winning glories in the wars and fighting heroically. As per the female heroes, they were featured rather with their beauties and attracting the male heroes. It was understood in the study that the magazine had a pedagogical objective and involved history teaching methods. The importance that the magazine attached to history teaching methods is understood from the author cadre of the magazine, which was comprised of history teachers, and the audience of the magazine, an important part of which was comprised of students and teachers. The result that the study put forth in the final analysis is that popular history magazines can be used in history education and teaching. However, while implementing this method, the ability should be acquired by the students to differentiate the mythos and the facts in the popular history magazines.

    AtıfTR Dizin1
    TR Dizinİncele
  • Proje2018
    Sülfanilamid Bağlanmış Manyetik Nanopartiküllerle (Fe 3 O 4 @SiO 2 -PA-SA) Karbonik Anhidraz Enziminin Saflaştırması

    Çelik, Safinur; Mavi, Ahmet

    Enzimler, endüstriyel süreçlerde ve bilimsel çalışmalarda oldukça yaygın olarak kullanılmaktadır. Bu sebeple enzimlerin hızlı ve ucuz bir yolla yüksek saflıkta saflaştırılması, ekonomik bir önem arz etmektedir. Bu projede amaç, karbonik anhidraz (CA) enzimini kısa sürede yüksek verimle saflaştırmak için ucuz, tekrar kullanılabilir ve manyetik temelli yeni bir materyal geliştirmektir. Projenin birinci bölümünde ilk olarak Fe3O4 MNP sentezlenmiştir. MNP?nin kararlılığını sağlamak için ikinci basamakta tetraetilortosilikat (TEOS) kullanılarak MNP?ler silika ile kaplanmış ve Fe3O4@SiO2 elde edilmiştir. Üçüncü basamakta Fe3O4@SiO2 yüzeyine APTES ((3-Aminopropil) trietoksisilan) ile -NH2 grubu ilave edilerek Fe3O4@SiO2-NH2 üretilmiştir. Dördüncü basamakta ise Fe3O4@SiO2-NH2 yüzeyinde bulunan -NH2 ile Phloretik asitin (PA, 3-(4-hidroksifenil) propiyonik asit) -COOH grubu, EDC (N-(3- Dimetilaminopropil)-N?-etilcarbodiimid hidroklorid) ve NHS (N-hidroksi süksinimid) aktivatörlüğünde, kovalent olarak bağlanmış ve Fe3O4@SiO2-PA elde edilmiştir. Son olarak karbonik anhidraz enziminin ilgisinin bulunduğu sülfonamidin (SA) diazonyum tuzu, Fe3O4@SiO2-PA?de bulunan Phloretik asitin aromatik halkasına bağlanarak Fe3O4@SiO2- PA-SA sentezlenmiştir. Nihai partikül (Fe3O4@SiO2-PA-SA) SEM, TEM, XRD ve FT-IR ile karakterize edilmiştir. TEM analiz sonuçları, hekzagonal şekle sahip monodispers ve ortalama 10 nm boyutlarında Fe3O4 MNP?ler üretildiğini göstermektedir. Silika kaplama ve APTES modifikasyonu yapılan nanopartiküllere ait TEM görüntüleri; Fe3O4@SiO2-NH2 MNP?lerin multi-çekirdek içerdiğini, SEM görüntüleri ise MNP?lerin 100 nm?den küçük ve küresel morfolojiye sahip olduğunu göstermektedir. Projenin ikinci bölümünde Fe3O4@SiO2-PA-SA kullanılarak hemolizattan karbonik anhidraz (CA) enzimi saflaştırılmıştır. Fe3O4@SiO2-PA-SA partikülleri hemolizat ile karıştırılıp CA?nın sülfanilamite bağlanması sağlanmıştır. Daha sonra bir mıknatıs ile Fe3O4@SiO2-PA-SA:::CA partikülleri toplanarak hemolizat ortamından uzaklaştırılmıştır. Fe3O4@SiO2-PA-SA:::CA partikülleri yıkanarak safsızlıklar ortamdan uzaklaştırılmıştır. Fe3O4@SiO2-PA-SA:::CA partiküllerinin üzerine elüsyon tamponu ilave edilerek CA, partiküllerden ayrılmış ve çözelti ortamına alınmıştır. Ortamda kalan Fe3O4@SiO2-PA-SA partiküller tekrar kullanılmak üzere mıknatısla geri kazanılmıştır. Sentezlenen MNP?ler ile CA, 2 saat gibi kısa bir zamanda ve basit bir şekilde hemolizattan saflaştırılabilmiştir. MNP?lerin maksimum enzim tutma kapasitesi 19,5 ± 1,1 mg CA/g MNP olarak hesaplanmıştır. Elde edilen CA?ın SDS-PAGE ile saflığı kontrol edilmiş ve enzimin yüksek saflıkta olduğu görülmüştür. Sentezlenen MNP?leri tekrar kullanılabilirliği test edilmiş ve üç kullanıma kadar MNP?lerin etkinliğini kaybetmediği görülmüştür.

  • MakaleYalnız künye2018
    Türkiye’de Cumhuriyetin İlanıyla Birlikte Yetiştirilecek Bireylere Kazandırılması Düşünülen Temel Nitelikler

    Duman, Tayyip; Demir, Fatih

    Türkiye’de 1923 yılında Cumhuriyetin ilan edilmesiyle siyasi, toplumsal ve ekonomik anlamda yeni bir devlet ve yönetim şekli hakim olmuştur. Bu yeni devlette eğitim üzerinde önemle durulan konuların başında gelmektedir. Osmanlı İmparatorluğunun özellikle son 150 yılındaki değişme ve gelişmelerin ardından 1923 yılında Türkiye’de “Cumhuriyet Rejimi” ne geçişle birlikte, Türk toplumunda yeni devlet anlayışı doğrultusunda yetiştirilecek bireylere kazandırılması düşünülen temel niteliklerde önemli ve köklü değişimlerin meydana gelmesi, Türk Toplumunun içinden geçtiği sürecin doğal bir sonucudur. Cumhuriyet idaresinin, eğitim yoluyla yetiştirilecek bireylere kazandırılmasını düşündüğü temel nitelikler, dönemin eğitim politikaları doğrultusunda yeni nesillerden beklentilerini ve devletin hedeflediği yeni insan modelinin özelliklerini ortaya koymaktadır. Bu araştırma, Cumhuriyetin ilanıyla yeni devlette yetiştirilecek bireylere kazandırılmasını düşünülen temel nitelikleri belirlemeyi amaçlamıştır. Araştırmada, Cumhuriyetin ilanıyla birlikte yetiştirilecek bireylere kazandırılması düşünülen 20 temel nitelik tespit edilmiştir. Cumhuriyetin ilanıyla birlikte takip edilen eğitim politikaları ile yetiştirilecek bireylere kazandırılması düşünülen temel nitelikler arasında ciddi bir ilişki olduğu görülmektedir.

    AtıfTR Dizin1
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  • MakaleYalnız künye2018Scopus SJRQ1
    Accurate and reliable U-value assessment of argon-filled double glazed windows: A numerical and experimental investigation

    Cuce, Erdem

    U-value assessment is a significant process to be able to evaluate the thermal insulation performance of building materials notably windows. About 60% of energy losses from building envelope is attributed to windows, hence accurate and reliable thermal resistance evaluation of glazed areas is of vital importance for a sensitive energy demand analysis of buildings. U-value assessment of windows is usually conducted through theoretical and numerical methods in literature. However, dwellers frequently complain about the insufficient thermal insulation performance of commercial glazing products when compared to the reported U-values in datasheets. This inconvenience arises from the lack of comprehensive experimental tests which simulate the actual operating conditions. Therefore, in this research, argon filled double glazed windows, which is one the most common fenestration products in market, is numerically and experimentally analysed in terms of U-value performance. Although computational fluid dynamics (CFD) analyses reveal that the U-value of the reference sample (4 mm pane + 20 mm argon + 4 mm pane) is 0.89 W/m(2)K, which is in good accordance with the theoretical data (0.80 W/m(2)K), it is observed from the environmental chamber tests that the U-value in simulated operating conditions is 1.25, 1.18 and 1.32 W/m(2)K for top, centre and lower positions of the window sample. The tests are repeated for accuracy verification, and the U-value is found to be 1.23, 1.18 and 1.31 W/m(2)K for the said points. In this respect, it is concluded that thermal bridges and edge effects play a key role in actual U-value performance of glazing products. Therefore, experimental performance figures are recommended to be utilised in energy demand analyses of buildings. (C) 2018 Elsevier B.V. All rights reserved.

    AtıfWoS72OpenAlex90
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  • MakaleYalnız künye2018Scopus SJRQ3
    THE COOPERATION BETWEEN METHYL JASMONATE AND SALICYLIC ACID TO PROTECT SOYBEAN (GLYCINE MAX L.) FROM SALINITY

    Seckin-Dinler, Burcu; Tasci, Eda; Sarisoy, Ufkun; Gul, Volkan

    Methyl jasmonate (MeJA), a methyl ester of jasmonic acid, is a naturally produced phytohormone that regulates plant growth and development by maintaining the morphologic, physiological, and biochemical development of plants. The purpose of this study was to determine the effect of methyl jasmonate and endogenous salicylic acid on the stimulation and scavenging of reactive oxygen species (ROS) under salt stress in soybean roots and leaves. Soybean leaves were sprayed with 0.5 mu M methyl jasmonate for 4 days, followed by exposure to 200 mM NaCl. Foliar application of MeJA did not change the leaf area; however, it increased the relative water content (RWC), chlorophyll content, relative electrolyte leakage (REL), and root and shoot fresh weight, and it lessened the stress-induced oxidative damage by decreasing the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA). Curiously, catalase (CAT) enzyme and isoenzyme activity, and also endogenous salicylic acid (SA) were decreased by MeJA treatment. As a result, this study firstly determined that MeJA treatment improved the soybean performance under salinity by acting role as an antioxidant and cooperating with SA.

    AtıfWoS4
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  • MakaleYalnız künye2018
    THE GENERAL SOLUTION OF A LAYERED MEDIUM RESTING ON A RIGID FOUNDATION

    Adiyaman, Gokhan; Öner, Erdal; Birinci, Ahmet

    In this study, the general solution of a receding contact problem of layered medium resting on rigid foundation is considered. The layered medium consists of N homogeneous layer with varying material properties and heights. The contact surfaces can be receding or attached. The problem may include M receding contact surfaces. The layered medium is loaded with n concentrated loads transmitted by circular stamps and r distributed loads. The problem is reduced to integral equation system using elasticity theory and Fourier integral transform. The numerical solution of the system is done by using Matlab. A program is written to solve the problem given by the user with any geometry and loading. The program is tested with two studies from the literature and it was shown that the results are compatible.

    AtıfWoS3
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  • MakaleYalnız künye2018Scopus SJRQ3
    THE EFFECT OF PROGRESIVE RELAXATION TRAINING ON FREE RADICALS, ANTIOXIDANT SYSTEM AND OXYDATIVE DNA DAMAGE

    Aggon, Eser; Ozturk, Nurinnisa; Alp, Hamit Hakan; Siktar, Elif; Agirbas, Ozturk; Bakan, Ebubekir

    This study aimed in sedentary subjected to progressive relaxation training (PRT) for determination of the effect of this exercise on MDA, OHdG, and NO levels and SOD and GPx activities. Experimental design was used in this research. Seven healthy, sedentary volunteers were included in the study. All subjects were male and within 15 to 18 years of age. Jacabson's Progressive Muscle Relaxation Training was applied to the volunteers for 6 six weeks. Before and after the chronic and acute exercise, blood samples were taken from the subjects, and K-Related Samples Friedman test was used for the analysis of repeated measurements and for the comparison of pre- and post-exercise data and chronic and acute mode data. In addition, 2-Related Samples Wilcoxon test was used in the evaluation of the groups as pairs. Our findings showed increases in MDA PRT, and no statistically significant increase in NO levels after PRT in sedentary volunteers. When the effect of relaxation exercise on the antioxidant system is considered, both SOD and GPx activities increased. The effect of PRT on 8-OHdG has been found to exhibit significant increase after PRT. It can easily be said that PRT may be helpful in patients with chronic disease, especially for obtaining a healing and a high quality sleep. In addition, in order for the athletes to feel well and to use their muscles actively, PRT may be helpful. We can conclude that, with respect to human health and physiology, no controversy may be seen in progressive muscle relaxation training.

  • MakaleYalnız künye2018Scopus SJRQ2
    Plant Origins of Propolis from Hakkari, Turkey

    Ecem Bayram, Nesrin; Sorkun, Kadriye; Cevahir Oz, Gul

    In this study, palynological analysis of 64 propolis samples from Hakkari province in Eastern Anatolia Region of Turkey was carried out. By microscopic analysis method, pollen diagnosis was made in propolis samples, and plants which may be propolis sources, were estimated. As a result, 71 plant taxa belonging to 34 families were identified at different rates in propolis samples. The pollens of the plants belonging to the family Apiaceae (2.7%-35%), Asteraceae (3.4%-35.4%) and, Fabaceae (12%-66%) were considerably found in the propolis of the Hakkari province. This study is the first report introducing the plant profile of Hakkari propolis in detail.

    AtıfWoS1
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  • MakaleYalnız künye2018Scopus SJRQ3
    CHEMICAL CHARACTERIZATION OF KARS HONEY

    Ozenirler, Cigdem; Ecem-Bayram, Nesrin; Gencay-Celemli, Omur; Celikbicak, Omur; Sorkun, Kadriye

    This study was undertaken to determine some chemical parameters (moisture, fructose/glucose ratio, hydroxymethylfurfural value, volatile compounds) of honey samples obtained from Kars. 100 honey samples from eight districts of Kars were evaluated. The chemical characterizations were as follows: average moisture (15,5 +/- 1,11%), HMF (0,77 ppm 0.9 ppm), F/G ratio (1,02 +/- 0,16). As a result of the gas chromatography mass spectrometry (GC MS) analyses, some volatile compounds in honey samples such as alcohols (1,15 +/- 1,84%), aldehydes (4,66 +/- 6,33%), aliphatic acids and their esters (1,86 +/- 4,5%), hydrocarbons (2,32 +/- 3,1%), flavonoids (4,34 +/- 1,63%), carboxylic acids and their esters (0,24 +/- 0,96%), ketones (6,05 +/- 6,35%), sugars (15,79 +/- 10,74%),vitamins (0,59 +/- 2,66%) were determined and quantitative variations of chemical components in different samples were reported.

    AtıfWoS3
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  • MakaleYalnız künye2018Scopus SJRQ3
    EFFECTS OF MENTHA PULEGIUM EXTRACT AND VITAMIN E SUPPLEMENTATION ON LAYING HENS PERFORMANCE, EGG QUALITY, ANTIOXIDANT ENZYME ACTIVITY IN SERUM

    Bolukbasi, Saziye Canan; Urusan, Hilal; Erhan, Muhammet Kuddusi

    The aim of this study was to investigate the effects of the supplementation of Mentha pulegium extract (MPE) and vitamin E on performance, egg quality and antioxidant enzyme activities of laying hens. The experiment study was conducted with 120 40 weeks old laying hens (Lohman LSL). Hens were given five dietary treatments of which each was repeated six times on 4 birds. Hens in the experiment groups were given 50 mg / kg MPE, 100 mg / kg MPE, 50 mg / kg vitamin E, 100 mg/kg vitamin E and the hens in control group were fed on basal diet. MPE and vitamin E supplementation did not make any change on feed consumption and egg weight but improved feed conversion ratio. Addition of 100 mg / kg MPE and vitamin E significantly increased the egg production. No significant difference were found between control and experimental groups in terms of shell thickness, yellowness, shape index and ratio of albumen, yolk and egg shell. However, MPE and vitamin E supplementation improved the shell breaking strength and Hough Unit. Dietary supplementation of MPE and vitamin E did not change AST, ALT, TG, cholesterol, HDL, LDL and glucose level in serum. Serum MDA value was decreased by MPE and vitamin E addition. Serum MDA levels were reduced by addition of MPE and vitamin E, but there was no difference in SOD activity among the groups. The addition of 50 and 100 mg / kg vitamin E significantly reduced the GSH-Px activity. As a result, 100 mg / kg MPE supplementation can be recommended as an alternative to antibiotic feed additives.

    AtıfWoS4
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  • MakaleYalnız künye2018
    THE MOST APPROPRIATE EARTHQUAKE RECORD GROUPS FOR DYNAMIC ANALYSIS OF A BUILDING

    Atmaca, Barbaros; Ergun, Mustafa; Ates, Sevket

    In this paper, seven real earthquake records are scaled according to Eurocode 8 design acceleration spectrum by using SESCAP (Selection and Scaling Program). SESCAP is a scaling program based on time domain scaling method and developed by using MATLAB, GUI software. Real and scaled earthquake records are used for linear time history analyses of a six-storied reinforced concrete building modeled as spatial by SAP2000. Eurocode 8 allows the use of real earthquake records for linear and nonlinear time history analyses of structures. In the case of using three earthquake records in linear and nonlinear time history analyses, maximum results of structural responses are used for design of structures. If at least seven time history analyses are performed, the mean responses of the structures are taken into account rather than the maximum results. For the selection of maximum results of structural response from thirty five groups are created by calculating combination of threes of seven real and scaled earthquake records, and another group including all of the seven real and scaled earthquake records are created for selection of mean. Relative floor displacements along X axis of the building are preferred as structural response of the building in this study. It is seen that differences between mean value and maximum value of the relative floor displacements along X axis of the building induced by seven and three scaled earthquake records respectively are less than ones obtained from real earthquake records.

  • MakaleYalnız künye2018Scopus SJRQ2
    Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine

    Hasanoglu Özkan, Elvan; Kizil, Hamit Emre; Sakiyan, Iffet; Nartop, Dilek; Sari, Nursen; Agar, Guleray

    The purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.

    AtıfWoS3
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  • MakaleYalnız künye2018Scopus SJRQ3
    Identification of Lactic Acid Bacteria from Spontaneous Rye Sourdough and Determination of Their Functional Characteristics

    Ispirli, Hümeyra; Demirbaş, Fatmanur; Yüzer, Mustafa O.; Dertli, Enes

    This study aimed to identify Lactic Acid Bacteria (LAB) present in rye and rye bran sourdoughs that were prepared spontaneously and determine their functional characteristics. Genotypic characterization of the isolates revealed the presence of 15 distinct LAB strains belonging to 6 different species; Leuconostoc citreum, Lactobacillus plantarum, Lactobacillus graminis, Lactococcus lactis subsp. cremoris, Weissella confusa, and Weissella cibaria in rye sourdoughs. PCR screening of eps genes required for exopolysaccharide (EPS) production revealed that all strains possessed at least one gene required for EPS production and all strains produced homopolymeric EPS comprised of glucose or fructose two Lactobacillus plantarum strains produced both glucan and fructan type EPS. Antifungal activity of these strains was tested against Aspergillus niger and Penicillium chrysogenum identified in this study and several patterns were observed. The phytase activity of these strains was also determined and all strains showed phytase activity with varying degrees.

    • SDG 2 · Açlığa Son
    AtıfScopus24WoS15OpenAlex24
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  • MakaleYalnız künye2018Scopus SJRQ4
    Partial Purification and Some Interesting Properties of Glutathione Peroxidase from Liver of Camel (Camelus dromedarius)

    Chafik, Abdelbasset; Essamadi, Abdelkhalid; Çelik, Safinur Yildirim; Solak, Kübra; Mavi, Ahmet

    Climate change and increasing temperatures are global concerns. Well adapted to desert life, the camel (Camelus dromedarius) lives most of its life under high environmental stress and represents an ideal model for studying desert adaptation among mammals. Glutathione peroxidase is the principal antioxidant defense system capable of protecting cells from oxidative stress. Glutathione Peroxidase from camel liver was purified (11.64-fold purification with 1.73% yield) and characterized The molecular weight of the enzyme was estimated to be about 69 kDa by gel filtration and 34 kDa by SDS-PAGE, implying dimeric structure of the protein. An optimum temperature of 47°C and an optimum pH of 7.8 were found. This enzyme is a typical SH-enzyme that is inhibited by D,L-dithiothreitol and β-mercaptoethanol and sensitive to bivalent cations. The enzyme had common specificity toward hydroperoxides and high specificity for reduced glutathione. The Km and Vmax values for hydrogen peroxide and reduced glutathione were 0.57 and 2.10 mM and 1.11 and 0.87 U/mg, respectively. The purified enzyme contained 16 ng of selenium per mg of protein. Our results show that the camel glutathione peroxidse exhibits properties different of those reported for other mammalian species. Lower molecular weight, homodimeric structure, higher optimum temperature, relatively low optimum pH, high affinity for hydrogen peroxide at low concentration of reduced glutathione and very low content of selenium could be explained by adaptation of the camel to living in the desert under intense environmental stress.

    AtıfScopus12WoS9OpenAlex11
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  • MakaleYayıncıda açık erişim2018Scopus SJRQ2
    Optimization of Adsorption Parameters for Ultra-Fine Calcite Using a Box-Behnken Experimental Design

    Uçurum, Metin; Özdemir, Akln; Teke, Çaǧatay; Serencam, Hüseyin; Ipek, Mümtaz

    Removal of heavy metals from wastewater is a significant issue because it prevents environmental-based concerns and impacts a large number of diseases and disorders. Many low-cost natural materials have been offered recently as possible precursors to commercial synthetic adsorbents. Ultra-fine calcite, one of these natural materials, has been investigated as a potential commercial adsorbent. Response surface designs are effective experimental techniques to investigate the heavy metal adsorption capacity of ultra-fine calcite. In the present study, one such response surface design, Box-Behnken, is used in order to optimize adsorption factors, such as pH level, initial metal concentration, stirring rate and adsorption time, and to determine the heavy metal capacity of this adsorbent. Our results show that the proposed methodology is an effective approach to optimizing the adsorption process and to maximize the heavy metal capacity.

    • SDG 6 · Temiz Su ve Sanitasyon
    • SDG 12 · Sorumlu Üretim ve Tüketim
    AtıfScopus9WoS7OpenAlex12
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