Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Can, Zehra(4)
- Dertli, Enes(3)
- Akar, Zeynep(2)
- Bülbül, Ali Savaş(2)
- Demir, Nazan(2)
- Demir, Yaşar(2)
- Gerçek, Yusuf Can(2)
- Kantar, Naciye Kutlu(2)
- Kara, Yakup(2)
- Turan, Metin(2)
- Çakır, Özlem(2)
- Çelik, Safinur Yıldırım(2)
- Agar, Guleray(1)
- Akar, Bülent(1)
- Alim, Zuhal(1)
- Alkan, Ozan(1)
- Arslaner, Ayla(1)
- Ates, Fatih(1)
- Avcı, Sibel(1)
- Barış, Özlem(1)
- Bayat, Ramazan(1)
- Bayir, Yasin(1)
- Bayraktar, Bülent(1)
- Bayram, Nesrin Ecem(1)
- Bayram, Sinan(1)
- Baştürk, Fatma Nur(1)
- Bekmezci, Muhammed(1)
- Beydemir, Şükrü(1)
- Bostancı, Maşide Temiz(1)
- Bozdeveci, Arif(1)
- Cebeci, Fatma(1)
- Demir, Elif Ayazoglu(1)
- Demir, Çağlar(1)
- Dogan, Hanife(1)
- Ecem Bayram, Nesrin(1)
- Erdem, Gizem Melissa(1)
- Erdogan, Ummugulsum(1)
- Erdoğan, Yaşar(1)
- Ergen, Berivan(1)
- Erten, Tayyibe(1)
Yayınlar
- MakaleYalnız künye2018Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes
Isgor, Mehmet Mustafa; Kılınç, Namık; Alim, Zuhal; Beydemir, Şükrü; Sengül, Bülent
Carbonic anhydrase enzyme plays a vital role in metabolic events such as acid-base regulation and respiration. Inour research, it is tried to determine the inhibitory influences of the cardiovascular therapeutics esmololhydrochloride, amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes carbonic anhydrases(hCA I and II). In accordance with this purpose, carbonic anhydrase isoenzymes were purified from humanerythrocytes by using affinity chromatography method. Enzyme purity was checked by SDS-PAGEelectrophoresis method. After the carbonic anhydrase enzymes were purified, the inhibitory affects ofcardiovascular therapeutics on these enzymes, using esterase activity, which is the method of measuring in vitroactivity, were examined. The three cardiovascular therapeutics dose-dependently decreased activity of hCAs. IC50values of amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride were found to be,respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM, 3.5 mM, and 6.36 mM for hCA-II. Our resultsproved that, under in vitro conditions, cardiovascular therapeutics significantly inhibit human CA-I and IIactivities. So, irregular use of these medicines may cause serious adverse effects in terms of human health.
AtıfTR Dizin2
İncele - MakaleAçık Erişim2018Investigation of the Effect of Some Herbal Extracts Used in Different Ratios on Serum Iron Level in Experimentally Heat Stress Created in Broiler
Bayraktar, Bülent; Tekce, Emre
Objective: Iron is found in the structure of hemoglobin, which carries oxygen to tissues with a critical prescription for life. Hemoglobin is a protein involved in iron binding and transport, which can lead to significant morbidity, leading to impaired suppression of the immune system and resistance to infections, as well as impaired brain and motor function. Material and Methods: Iron level in body; there were studies showing that consumption of iron-containing foods, use of certain hormones and medicines, and stress-related effects have been seen. On the other hand, in psychological and heat stress studies that experimentally applied in rats, it has been reported that levels the level was decreasing. In this study, it was aimed to determine the change in serum iron levels by adding essential oil mixture (EOM) with antioxidant effect to drinking water in 22°C and 36°C heat stress in chickens which are the most sensitive animals to stress. In this study, 400 pcs and 1 day old Ross-308 male broiler chicks were used. There are 8 different groups with 50 animals in each group, it will be divided into 10 pcs in each section and the study was carried out from the 15th day to the 42nd day. The study lasted for 42 days, for 22°C stress-free and 36°C stressed groups with 5 subgroups separated (Control, EOM (essential oil mixture)-250, EOM-500, EOM-750. Results: Serum iron levels in the experimental groups were 88.2, 82.2, 103.4, and 98.4 μg/dL at 22°C, respectively, and 88, 78.2, 84.8, and 85 g/L respectively at 36°C stressed broiler groups. Conclusion: It has been determined as a result of this study, serum ıron levels not affected by the EOM added to drinking water (p>0.05).
AtıfOpenAlex6İncele - MakaleYalnız künye2017The In-vitro Effect of Lisonopril on Serum Alkaline Phosphatase and Acid Phosphatase Enzymes Activity
Demir, Yaşar; Çelik, Safinur Yıldırım; Demir, Nazan
Hypertension is a disease that most elderly people suffer. People with high blood pressure often obliged to use the drug that lowers blood pressure in order to maintain a healthy lifestyle throughout their lives. In this study, the in vitro effect of lisonopril on serum alkaline phosphatase and acid phosphatase enzymes which are essential for normal bone formation and mineralization activities was investigated. Alkaline phosphatase enzyme was purified using DEAE-cellulose, acid phosphatase enzyme was purified using CM-cellulose ion exchange chromatography from human serum. The activities of alkaline and acid phosphatase were determined using pnitrophenylphosphate as the substrate. When finding out the activity of TrACP enzyme, adding tartrate to the environment provided inhibition of other acid phosphatase enzymes than TrACP. The activities of alkaline phosphatase and acid phosphatase, which is resistant to tartrate, have been found at five different concentrations of substrates (0.033, 0.1, 0.16, 0.23 and 0.3 mm) in environment with inhibitor and without inhibitor. While three different concentrations of the inhibitor, including 10-2,10-3 and 10-4, was used for each substrate concentration. It was found that the drug inhibit both enzymes, and I50 value was calculated as 0.17 mm and 0.79 mm. Furthermore, Ki values were calculated as 6.98x10-4, 7.06x10-4 for ALP and TrACP respectively by utilizing the Lineweaver-Burk plot
- MakaleAçık Erişim2017Farklı Propolis Örneklerinin Ana Bileşenleri
Bayram, Nesrin Ecem; Gerçek, Yusuf Can
Propolis bal arıları tarafından bitkilerden toplanan reçinemsi bir maddedir. Bu çalışmanın amacı Hakkari (Türkiye)’den toplanan propolisin kimyasal içeriğini incelemektir. Bu amaçla farklı coğrafik özelliklere sahip yerlerden toplanan sekiz propolis örneğinin ilk olarak etanol özütleri hazırlandı ve daha sonra kimyasal bileşenlerinin yoğunluğu gaz kromatografisi ve kütle spektrometresi (GC-MS) aracılığıyla belirlendi. Sonuç olarak, hidrokarbonlar, alifatik asit ve esterleri, karboksilik asit ve esterleri, sinnamik asit ve esterleri, flavonoidler, alkoller ve terpenler propolis örneklerinde tespit edildi. Propolis 6’nın total flavonoid içeriği diğer örneklere kıyasla daha yüksek oranda bulundu. Ayrıca ‘Etil oleat’ bileşiği bütün propolis örneklerinde farklı miktarlarda saptandı.
AtıfOpenAlex7TR Dizin1İncele - MakaleYalnız künye2015Scopus SJRQ2Yoğurttan ekzopolisakkarit üreten streptococcus thermophilus suşunun izolasyonu ve tanımlanması
Dertli, Enes
Production of exopolysaccharides (EPS) by starter Lactic acid bacteria (LAB) gained special interest due to technological role of thesenatural polymers. Yogurt samples were collected from diferent households in Turkey and bacterial strains were isolated and evaluatedfor EPS production. Strains potentially producing EPS were then identified to species level with 16S rRNA sequencing. An EPS producerStreptecoccus thermophilus strain was identified from yogurt samples. EPS was extracted from culture supernatants of S. thermophilus andpartially purified and structural analysis of the crude EPS by FTIR spectroscopy revealed the presence of typical functional groups relatedto exopolysaccharides. This study explains the identification of a potential starter culture for yogurt production with EPS production.
AtıfTR Dizin4İncele - MakaleYalnız künye2015Agrobacterium radiobacter'den Pektin Liyaz Enziminin Üretilmesi, Saflaştırılması, Karakterize Edilip Meyve Suyu Üretiminde Kullanılması
Barış, Özlem; Demir, Yaşar; Yıldırım Çelik, Safinur; Demir, Nazan; Güllüce, Medine
Ekstraselüler pektin liyaz enzimi (EC 4.2.2.10) Agrobacterium radiobacter'den katı ortam fermantasyonu ile üretilmiş ve DEAE-selüloz iyon değişim kromatografisi kullanılarak 21 kat saflaştırılıp karakterize edilmiştir. Enzimin molekül ağırlığı Sephadex G-100 jel filtrasyon kromatografisi ile 67 kDa olarak bulunmuş ve enzimin saflığı SDS-PAGE ile kontrol edilmiştir. Enzimin optimum pH'sı ve sıcaklığı sırasıyla 8 ve 60°C olarak belirlenmiştir. Enzim aktivitesinin 24 saat boyunca kararlı olduğu en yüksek sıcaklığın 40°C'de olduğu gözlenmiştir. 50°C'de yaklaşık 4 saat boyunca, 60°C'de ise 1 saat boyunca %50 aktivitesini koruduğu bulunmuştur. Saflaştırılan enzimin Vmax ve KM'si Lineweaver-Burk grafiği kullanılarak 4.36 mg/mL and 303 µmol/L.dakika olarak hesaplanmıştır. Pektin liyaz enziminin aktivitesi üzerine metal iyonlarının etkisi incelenmiş ve Mg2+ ve Ca2+ iyonlarının enzimi kısmen, Fe3+, Zn2+, Cu2+ ve Hg2+ iyonlarının enzimi tamamen inhibe ettiği anlaşılmıştır. Co2+ (0,1 mM, 1 mM ve 10 mM) iyonunun ise enzimin az da olsa aktivitesini arttırdığı gözlenmiştir. Saflaştırılıp karakterize edilen enzim meyve suyu üretiminde kullanıldığında kontrole göre meyve suyu veriminde önemli artış görülmüştür.