Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2026Phytochemical profile, antioxidant potential, and cytotoxic effect of the ethanol extract of Micromeria fruticosa
Çakır, Özlem; Topcu, Kübra Cinar; Anlar, Pinar
In this study, the ethanol extract of dried Micromeria fruticosa (L.) Druce, commercially sourced from the Tortum Valley (Erzurum Province), was comprehensively analysed to evaluate its bioactive potential. Considering that geographical origin and botanical differences significantly influence phytochemical composition and biological activity, the use of locally sourced commercial samples provides valuable data for plant standardisation and comparative studies. The total antioxidant activity of the extract was determined as 8.84±0.10, 4.96 ±0.05, 34.64 ±0.69, and 18.27 ±0.11 mg TE/g dry weight by DPPH, FRAP, CUPRAC, and ABTS assays, respectively. The total phenolic content was 26.25 mg GAE/g dry weight, and LC-MS/MS analysis revealed a high quinic acid content (93.395 mg/g). ICP-MS analysis showed high levels of essential minerals (Na, Mg, K, Ca, Fe, and Al), while toxic elements such as Cd, Te, and Tl were below the limit of detection, and Pb and Ba were present at very low concentrations. Additionally, the extract significantly reduced A549 lung cancer cell viability in a dose-dependent manner. Overall, Tortum Valley–derived M. fruticosa represents a promising natural bioactive source, and this study highlights the impact of local botanical and environmental variations on the biological activity of the same plant species.
- MakaleAçık Erişim2026Scopus SJRQ2Bioactive profile and mineral composition of fruit extracts from four wild hawthorn (Crataegus spp.) species
Çakır, Özlem; Çınar Topçu, Kübra; Sefali, Abdurrahman; Bayyiğit, İshak; İlhan, Gülçe; Singh, Jyoti
This study compared the antioxidant capacity, total phenolic content (TPC), phenolic composition, lipid oxidation level, and mineral profiles of extracts obtained from four wild hawthorn species (Crataegus tanacetifolia, C. orientalis subsp. orientalis, C. orientalis subsp. szovitsii, and C. monogyna) collected from northeastern Türkiye. Significant interspecific variations were observed in DPPH, FRAP, and CUPRAC assays. While C. monogyna had the highest reducing capacity and TPC, C. orientalis subsp. szovitsii had the highest DPPH activity. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry analysis showed that catechin, epicatechin, chlorogenic acid, and quinic acid were the major phenolics, and were particularly abundant in C. monogyna. Despite having a high phenolic content, C. monogyna had the highest malondialdehyde level, indicating that phenolics alone were insufficient to fully inhibit lipid oxidation. Inductively coupled plasma-mass spectrometry mineral analysis detected high levels of K, Mg, and P in all species. Principal component analysis effectively distinguished the species based on their antioxidant, phenolic, and mineral profiles. Overall, hawthorn fruits were confirmed as valuable sources of natural antioxidants and essential minerals.
AtıfScopus1WoS1
İncele - MakaleYayıncıda açık erişim2026Scopus SJRQ1Optimization of extraction conditions of Micromeria fruticosa and use of optimized extract in beef patties production
Anlar Daldal, Pınar; Cinar Topcu, Kübra; Çakır, Özlem
This study aimed to optimize the conditions for obtaining an ethanol extract from Micromeria fruticosa (M. fruticosa) and to determine the antioxidant activity and phenolic compound content of the resulting extract. Subsequently, the use of the optimized extract in beef patties was evaluated, and the effects of the cooking process on the physicochemical properties of the product were examined. In this context, the highest efficiency was obtained in terms of total phenolic contents (TPC), as well as DPPH (2,2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) values, during the ethanol extraction of the plant. The optimum extraction conditions were determined to be 12 g of sample, 20 min, and 75% ultrasonic power. Under these conditions, the TPC of the extract was found to be 37.128 mg GAE/g dry weight, the DPPH value was 42.865, and the FRAP value was 254.776 mg TE/g dry weight. In the second phase of the study, the optimized extract was used at different concentrations (0%, 0.5%, 1.0%, and 1.5%) to produce beef patties, which were then analyzed for pH, moisture content, TBARS (thiobarbituric acid-reactive substances), and cooking yield. The use of the extract did not cause a statistically significant difference in the moisture content and TBARS values of the samples (p > 0.05). However, it was determined that the pH values of the samples containing 0% and 0.5% extract were lower than those containing 1.0% and 1.5% extract (p < 0.01). In addition, it was observed that the use of the extract had a positive effect on cooking yield, and that cooking yield increased with increasing extract concentration. As a result of the study, it was concluded that M. fruticosa holds promising potential as a natural additive in meat and meat products, offering a functional ingredient that can contribute to the development of value-added, health-oriented food products.
- MakaleYalnız künye2025Antioxidant capacity, phenolic compound content, and mineral composition of ethanol extracts from sorbus aucuparia and sorbus kusnetzovii
Sefali, Abdurrahman; Çakır, Özlem; Topcu, Kübra Cinar; Anlar, Pinar
Ethanol extracts of Sorbus aucuparia L. and Sorbus kusnetzovii Zinserl. fruits, belonging to the Rosaceae family, were obtained using an ultrasonic-assisted extraction method. The total phenolic contents of S. aucuparia and S. kusnetzovii were 8.44±0.66 mg GAE/g and 8.65±2.18 mg GAE/g, respectively, while their total flavonoid contents were 2.30±0.01 mg QE/g and 2.67±0.13 mg QE/g, respectively. The fruits’ DPPH values were measured as 9.59±0.00 mg TE/g and 9.28±0.05 mg TE/g, FRAP values as 9.51±0.42 mg TE/g and 13.38±0.52 mg TE/g, and CUPRAC values as 13.43±0.01 mg TE/g and 23.21±0.74 mg TE/g. Furthermore, both Sorbus species were found to have a rich mineral content. Therefore, it can be concluded that S. aucuparia and S. kusnetzovii fruits contain potent antioxidants, abundant phenolic compounds, and valuable minerals. Furthermore, they have significant potential as functional foods and are strong candidates for further clinical research.
- MakaleAçık Erişim2024The Effect of Cadmium and Lead Exposure on the Development and Physical Structure of Quinoa (Chenopodium Quinoa Willd.)
Çakır, Özlem; Yazici, Abdullah; Gül, Zeynep
The presence of soil affected by Cd and Pb and their metals is increasing daily. Quinoa is a plant that can grow in harsh conditions due to being a halophyte plant. This study was planned to examine the effects of lead and cadmium metals, two of the most common metals today, on plant growth, physiology and some biochemical properties of quinoa. Within the scope of the study, heavy metal applications were made as 1 control (no application), 4 doses of Cd (50, 100, 150 and 200 mg/kg), and 4 doses of lead (500, 1000, 1500, 2000 mg/kg). In this study, which was carried out in Atatürk University, Plant Production and Application Center greenhouse conditions, it is observed that the metals applied negatively affected the parameters in the plant, and cadmium metal had a more toxic effect than lead metal. It is determined that the fresh weight of the plant lost 62% at the Cd 200 level and 45% at the Pb 2000 level compared to the control group.
AtıfOpenAlex1İncele - MakaleAçık Erişim2024Berberis Fruit Extracts in Marinated Meat: Quality Effects
Çakır, Özlem; Topcu, Kübra Cinar; Fettahoğlu, Kübra
In this study, the effects of the fruit extracts Berberis vulgaris L. and Berberis crataegina DC. on the quality of the meat were evaluated. According to the results of antioxidant activity analyses, the antioxidant activities of B. vulgaris and B. crataegina fruit extracts were determined as 81.29 ± 14.14% and 78.97 ± 0.39%, respectively. The phenolic content of B. vulgaris fruit extract was found to be 1707.88 ± 60.10 mg GAE/g, while the phenolic content of B. crataegina fruit extract was 83.64 ± 1.61 mg GAE/g (p<0.01). These differences indicate significant diversity in phenolic compounds among plant species. pH values of meat samples marinated with B. vulgaris extract showed a gradual decrease over time. For instance, at the 3rd hour, the pH value of meat samples marinated with B. vulgaris was determined as 4.42 ± 0.05 (p<0.01). At the end of storage, TBARS values were determined as (1.54±0.13; 0.41±0.11; and 0.19±0.02 mg MDA/kg) in the control, B. crataegina and B. vulgaris groups, respectively (p<0.01). A significant reduction in Total Aerobic Mesophilic Bacteria (TAMB) count was observed in meat samples marinated with B. vulgaris extract. For example, at the end of the 12th day, the TAMB count was determined as 8.95 ± 0.07 log CFU/g in the control group, while it was 5.74 ± 0.06 log CFU/g in meat samples marinated with B. vulgaris extract (p<0.01). These data demonstrate the potential of Berberis vulgaris and Berberis crataegina fruit extracts to improve meat quality. With their antioxidant activities, phenolic contents, pH effects, and microbial inhibition, these extracts can be considered important natural components to use in the meat industry.
AtıfOpenAlex1İncele - MakaleAçık Erişim2024Scopus SJRQ2Optimization of pectin extraction from crab apple peel and usage in a model meat emulsion system
Aldemir, Hazal; Kamiloğlu, Aybike; Çakır, Özlem
The aim of this study was to optimize conditions of microwave-assisted pectin extraction from crab apple peels using the Box–Behnken experimental design. For this purpose, microwave-assisted pectin extraction was performed at different extraction time (3, 6, and 9 min), liquid/solid ratio (75, 100, and 125), and pH (1.5, 2.0, 2.5). The optimum conditions were selected as extraction time (7.77 min), liquid/solid ratio (77.53 mL/g), and pH/1.79). The response, obtained under optimum conditions was determined as 0.1128 ± 0.0137 g pectin/g crab apple peel. In addition, it was determined that the obtained pectin sample had an esterification degree of 79.14% and oil and water binding capacities of 8.5 ± 0.7 g oil/g pectin and 8.1 ± 1.5 g water/g pectin, respectively. It was determined that pectin emulsions showed higher stability at 1% pectin level. The possibilities of using pectin obtained under optimized conditions as a fat replacer in a model sausage system were investigated. Pectin was evaluated in the model sausage system at two different concentrations. With the increasing pectin concentration, the cooking loss value increased from 14 to 18%, while leakage decreased from 0.31% to 0.18%, showing a negative effect on cooking loss and a positive impact on the amount of leakage into the packaging. Pectin obtained from crab apple peel has the potential to be used for reducing fat content in sausage environment.
AtıfScopus15WoS12OpenAlex14İncele - MakaleAçık Erişim2021Determination of Antioxidant Capacity, Total Phenolic Content and Phenolic Acid Profiles of Some Wild Fruit
Dertli, Enes; Çakır, Özlem; Tüysüz, Büşra
In this study, it was aimed to determine the phenolic substance profiles and antioxidant activities of wild fruit species such as, wild pear, hawthorn, cornelian cherry, firethorn which grow naturally in Turkey. For this purpose, the samples dried in the drying oven has been used in extraction. Total phenolic content was determined by FCR method, antioxidant capacity was determined by DPPH, CUPRAC, ABTS and β-carotene bleaching methods. The phenolic profiles of the fruits were illuminated by HPLC. Total phenolic content of the fruits was determined as 493 (wild pear), 847 (hawtorn), 823 (cornelian cherry), 2555 (firethorn) mg GAE/100 g. The results of antioxidant capacity of the wild pear, hawtorn, cornelian cherry and firethorn were determined as 12.275, 22.754, 47.705, 83.134 %TAC in DPPH method, 0.258, 0.619, 0.684, 2.871 mmol TE/g in CUPRAC, 73.78, 49.33, 51.56, 79.34 % TEAC in ABTS and 93.63%, 96.67%, 88.70%, 97.18% in β-carotene bleaching method, in respect. The fruits mainly contain chlorogenic acid, caffeic acid, vanillic acid and synapic acid.
AtıfOpenAlex8TR Dizin3İncele - MakaleAçık Erişim2021Yabani Kara Kuşburnu (R. pimpinellifolia L.) Meyvesinin Mineral İçerik ve Biyoaktif Bileşenler Açısından Değerlendirilmesi
Çakır, Özlem; Ergen, Berivan
Bu çalışmada Bayburt ilinde doğal olarak yetişen yabani R. pimpinellifolia L. meyvesinin genel özellikleri araştırılmıştır. Bu meyveninantioksidan özellikleri dört farklı yöntem (DPPH, ABTS, CUPRAC ve β-karoten ağartma) kullanılarak hem su hem de metanolekstrelerinde belirlenmiştir. Toplam fenolik madde içeriği ve fenolik profili sırası ile FCR (Folin-Ciocalteu ayıracı) metodu ve HPLCyardımıyla analiz edilmiştir. Bunlara ilaveten mineral madde içeriği ve bazı fizikokimyasal özellikleri ele alınmıştır. Su ekstresindemeyve antioksidan seviyeleri CUPRAC yönteminde 0.56 mmol TR/g-örnek, β-karoten ağartma yönteminde %97.02, ABTS metodunda%88.65 ve DPPH metodunda ise %86.69 olarak tespit edilmiştir. Metanol ekstresinde ise CUPRAC yönteminde 0.99 mmol TR/g-örnek,β-karoten yönteminde %95.21, ABTS metodunda %93.43 ve DPPH metodunda ise %85.21 olarak tespit edilmiştir. Toplam fenolikbileşen seviyesi ise sulu ekstresinde 929.27 mg GAE/100g olarak belirlenmiş olup bu değer metanol ekstresinde elde edilendenyüksektir. Meyvenin içerdiği fenolik bileşenler arasında en baskın olanların sırası ile klorojenik asit, vanilik asit ve gallik asit olduğubelirlenmiştir. Meyvede bulunan minerallerin arasında en yoğun olanlar sırası ile K (12.1688 mg/ml), P (1.3429 mg/ml) ve Mg (1.1926mg/ml) olurken en az bulunan mineralin ise Cr (0.00001 mg/ml) olduğu açığa çıkartılmıştır. Sonuç olarak kara kuşburnu meyvesinin(R. pimpinellifolia) sahip olduğu bu özelliklerden dolayı çok iyi bir doğal besin olarak değerlendirilebileceği anlaşılmıştır.
AtıfOpenAlex3TR Dizin2İncele - MakaleYalnız künye2020Yabani-Ekşi Elmanın (Malus sylvestris Miller) Antioksidan Aktivite ve Fenolik Madde İçeriği Üzerine Farklı Kurutma Yöntemlerinin Etkisi
Çakıroğlu, Kübra; Güldemir, Kübra; Çakır, Özlem
Bu çalışma kapsamında, Bayburt çevresinde yetişen yabani ekşi elma (Malus sylvestris Miller) örneği dört farklıkurutma yöntemi (infrared kurutma, sıcak havada kurutma, oda sıcaklığında kurutma ve dondurarak kurutma) ile kurutulmuşve kurutulan ürünlerin fenolik madde içeriği ve antioksidan aktiviteleri belirlenmiştir. Kurutma yöntemlerine göre toplamfenolik madde içerikleri açısından en düşük değer oda şartlarında kurutulmuş elma örneğinde (34.92 mg GAE/g KM), enyüksek değer ise sıcak havada kurutulmuş örnekte (41.18 mg GAE/g KM) tespit edilmiştir. Yabani ekşi elma örneklerininantioksidan aktivite seviyeleri; FRAP yöntemine göre “dondurarak kurutma > sıcak havada kurutma > infrared kurutma >oda sıcaklığında kurutma” şeklinde, CUPRAC yöntemine göre “sıcak havada kurutma > dondurarak kurutma > infraredkurutma> oda sıcaklığında kurutma” şeklinde, β-karoten yöntemine göre ise dondurarak kurutma>sıcak havada kurutma>odasıcaklığında kurutma>infrared kurutma şeklinde değiştiği belirlenmiştir. CUPRAC metodu ile FRAP metodu arasında yüksekbir korelasyon olduğu saptanmıştır (r=0.939, R2=%88). Elde edilen deneysel verilere göre, antioksidan ve fenolik maddeiçeriği açısından yabani ekşi elma (Malus sylvestris Miller) için en iyi kurutma yönteminin sıcak havada kurutma olduğutespit edilmiştir. Renk değerleri açısından en iyi yöntemin dondurarak kurutma olduğu belirlenmiştir.
AtıfTR Dizin3İncele - MakaleAçık Erişim2020Scopus SJRQ2Comparison of two wild-grown Berberis varieties based on biochemical characterization
Çakır, Özlem; Karabulut, Abdulkadir
In this study, some physicochemical properties, antioxidant activity, total phenolic content, mineral content, and phenolic profiles of Berberis vulgaris and Berberis crataegina wild-grown in Bayburt are analyzed and compared. The level of total phenolic content in B. crataegina DC. (73.48 µg GAE/mg KM) was found higher than B. vulgaris (71.54 µg GAE/mg KM). According to three methods (β-carotene, 2,2-diphenyl-1-picrylhydrazyl, and cupric reducing antioxidant capacity) the antioxidant activity of the B. crataegina is also higher. The change in color (L*, b*) is found statistically important (p <.05). There are no meaningful differences between the dry matter, C vitamin, pH value, and water activity (p >.05). It was observed that the fruit type has a great effect on brix and ash content (p <.05) and glucose, total sugar, and malic acid content. Chlorogenic acid is the dominant phenolic component for both types. It is followed by syringic, sinapic, and gallic acid in B. vulgaris and sinapic, syringic, and gallic acid in B. crataegina. Practical applications: Wild fruits have been consumed for centuries in nutrition or pharmacology applications. They are known as rich resources of antioxidant and phenolic compounds. For that reason, wild fruits play an important role in the prevention and treatment of some diseases especially cardiovascular diseases and cancer. In this study, wild-grown B. vulgaris and B. crataegina were analyzed in detail according to their bioactive properties and an assessment was made in terms of industrial and medical usability. This study reports mineral content, antioxidant activity, total phenolic content, and the phenolic acid profile (sinapic, syringic, gallic, chlorogenic, caffeic, vanillic, trans-ferulic, p-coumaric, and 4-hydroxybenzoic acids) of two Berberis varieties. According to results, these fruits may be used as natural flavoring and coloring material in food sector. Thanks to the active ingredients and minerals; these fruits may be used in pharmaceutical industry. They have a good potential for commercial exploitation and positive effects on health.
AtıfScopus16WoS8OpenAlex16İncele - Derleme MakaleYalnız künye2018Biyodinamik Tarım ve Organik Tarımın Karşılaştırılması
Çakır, Özlem; Karatas, Neva; Yildiz, Hilal
Modern tarımsal uygulamalar ürünlerin dayanıklılık ve verimliliğini en üst düzeyeçıkarmak için farklı biyolojik, fiziksel ve kimyasal işlem basamakları içerir. Bu işlemlerarasında özellikle kimyasal girdi kullanımının giderek artması sonucunda ortaya çıkançevresel etkiler konusundaki endişeler nedeniyle, modern tarımsal uygulamalar içinalternatif felsefeler ortaya çıkmıştır. Bu alternatif yaklaşımlar birçok yönden gelenekseluygulamalara benzerken göze çarpan birkaç farklılık ile gelenekselden ayrılmaktadır. 18.yüzyılın sonlarında başlayan endüstriyel devrimle birlikte, artan nüfus ve küresel ısınma,gezegenimizin bozulduğunun farkına varılmasına neden olmuş ve günümüzde organik vebiyodinamik gibi alternatif tarım metotları ile insan, çevre, su ve toprakların korunmasıgündeme gelmiştir. Biyodinamik tarım; 1924'te filozof Rudolf Steiner ve agronom E.Pfeiffer tarafından kurulan bir sistem olup insan-doğa-evren kavramını esas alanantropolojik bir teoriye dayanmaktadır. Biyodinamik tarım birçok açıdan daha iyi bilinenorganik tarıma benzer ve her ikisi de temel olarak kimyasal girdi (gübre, böcek ilacı,herbisit, hormon) kullanımına olan felsefi muhalefetleriyle şekillenmiştir. Biyodinamiktarımın organik tarımdan farkı, felsefi ve tarihi yönleri dışında, hayvansal organlarlafermente edilen özellikli bitki veya mineraller içeren biyodinamik preparatlarınkullanımında yatmaktadır. Bu derlemede, biyodinamik tarım uygulanmasının avantajlarıortaya konularak iki sistem (organik ve biyodinamik) arasındaki farklara yer verilecektir.
AtıfTR Dizin2İncele
