Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2026Scopus SJRQ4Microwave assisted green synthesis of nano-sized biogenic silver in extract of Phlomis linearis: potential anticancer and antimicrobial activities
Orçan Çayır, İlayda; Tahta, Beyhan; Cömertpay, Sabahattin; Uruş, Serhan; Bülbül, Ali Savaş; Aygan, Ashabil; Aksan, Mehmet Emrah
Silver nanoparticles (AgNPs) are known for their significant anticancer and antimicrobial activities in the field of medicine. In the present study, AgNPs were synthesized using a microwave-assisted green synthesis process, in which Ag(I) ions were reduced by organic compounds extracted from the leaves and stems of Phlomis linearis, an endemic plant which acted as both reducing and stabilizing agents. Although various species of the Phlomis genus have been studied in the literature, the synthesis of the nano-sized biogenic silver with reducing Ag(I) in the extract of the endemic plant P. linearis is reported here for the first study time, along with the evaluations of its bioactive properties. The obtained AgNPs were characterized by optical, structural, morphological, etc. techniques such as Ultraviolet–visible spectroscopy (UV–Vis.), Fourier-Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), and photoluminescence (PL). Gas chromatography − mass spectrometry (GC–MS) and FT-IR techniques were used in order to evaluate the biochemical-reduction mechanism. When their antimicrobial activities were determined by the well diffusion method on six bacterial strains, the results showed that P. linearis exhibited antimicrobial activity against all tested bacteria. Additionally, the investigation of the anticancer activity of the particles by using the MTS method on a breast cancer cell line (MCF-7) and a healthy human cell population (human umbilical vein endothelial cells [HUVEC]) revealed that our AgNPs were more cytotoxic toward the cancerous cells compared to the non-cancerous ones (p < 0.001).
- MakaleAçık Erişim2025Scopus SJRQ3Seed morphology of some Eremogone species (Caryophyllaceae) from Türkiye
Tokuşoğlu, Kader Varlik; Armağan, Metin; Bülbül, Ali Savaş
The present research deals with the taxonoy of the genus Eremogone, using morphology of seed; the species occurring in Türkiye are examined. Nineteen characters (qualitative and quantitative) were observed or measured using light microscopy and Scanning Electron Microscopy; statistical analyses considered are PCA and Clustering. The average length of Eremogone seeds was 1756 μm (1168–2569 μm), and the average width was 1148 μm (866–1674 μm). Seed shape, based on the seed length-to-width ratio, ranged from spherical to prolate spheroidal. Seed colors varied from yellowish-brown to dark brown-black. The seed coat surface was covered with tubercles, and the dorsal surface of the seed was covered with papillae ranging from 27 to 146 in length. The identified potential characters contribute to the delimitation of the Turkish species of Ermogone; a diangostic key is proposed.
- MakaleAçık Erişim2024Investigation of the relationship between orthorexic tendencies and some anthropometric measurements and variables in university students
Erkılıç, Tugçe Orkun; Bayraktar, Bülent; Bülbül, Ali Savaş; Bozok, Ümmü Gülşen; Erkılıç, Ali Ozan; Ozcan, Gulbahar Boyuk; Turan, Merve
Orthorexia nervosa (ON) is a type of eating disorder marked by an excessive preoccupation with eating healthily to preserve and promote health, which surpasses normal levels of concern. This study aims to investigate the correlation between orthorexic tendencies, anthropometric measurements, and several other characteristics among university students. A cross-sectional study was done with a sample size of 307 university students from various departments, consisting of 228 females and 79 males. The data-gathering process consisted of administering a demographic information form and the ORTO-11 questionnaire. Anthropometric measurements, such as weight, height, waist circumference, hip circumference, and chest circumference, were measured. In addition, Body Mass Index (BMI) was calculated. The TANITA BC-418 equipment was used to measure the weight of the participants, while a flexible tape measure was used to measure their height, waist circumference, hip circumference, and chest circumference. The BMI was calculated by the individual's weight (in kilograms) by the square of their height (in meters). The data analysis encompassed the utilization of descriptive statistics, t-tests, variance analysis, and Pearson correlation analysis. A score of 25 or lower on the ORTO-11 scale was categorized as orthorexic, whereas a score higher than 25 was deemed normal. The average age and orthorexia scores of the subjects were 20.81±1.86 and 27.76±4.42 for females, and 21.38±4.14 and 28.11±4.37 for males, respectively. It was determined that 29.3% of the participants were found to display orthorexic tendencies, while the remaining 70.7% did not. There were no statistically significant variations in age, hip circumference, and orthorexia scores across genders (p>0.05). No statistically significant relationship was found between the total orthorexia scores and BMIs of the individuals (r=-.051, p>0.05). ON is associated with psychological and social complications, which in turn contribute to the development of disordered eating patterns. The results of this study indicate that determining the frequency of orthorexic tendencies among university students may contribute to the development of strategies for its prevention.
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İncele - MakaleAçık Erişim2024Scopus SJRQ4SILVER AND ZINC NANOPARTICLES BIOSYNTHESIS USING LAUREL EXTRACT AND INVESTIGATION OF THE PHOTOCATALYTIC PROPERTIES
Taş, Recep; Köroğlu, Ebru; Karakuş, Ahmet; Bülbül, Ali Savaş; Taş, Nilay Akkuş
Metal nanoparticles that are widely studied in optoelectronics, catalysis, medicine, and sensors offer remarkable optical and electronic properties. To address the cost and environmental concerns associated with their synthesis, this study employs an environmentally friendly method using Laureus nobilis extract to produce silver and zinc nanoparticles, which are prominent in nanotechnology. This study includes investigations of factors such as reaction time, AgNO3/laurel ratio, Zn(Ac)2 H2O/laurel ratio and temperature in nanoparticle biosynthesis to optimize the process. The next stage was set to evaluate the photocatalytic performance of these nanoparticles, specifically against the methylene blue dye under dark and UV light conditions. Parameters such as pollutant decomposition, degradation rate, catalyst stability, and nanoparticle recovery were analysed. Structural characterization of the obtained nanoparticles was performed using UV-Vis, FTIR, SEM, and XRD techniques. The photocatalytic results showed significant degradation percentages for LB-AgNP (silver nanoparticles synthesized with Laureus nobilis extract) (97.5%) and LB-ZnNP (zinc nanoparticles synthesized with Laureus nobilis extract) (90.9%). LB-ZnNP showed superior performance. Therefore, LB-AgNP and LB-ZnNP are promising photocatalysts for water purification and the elimination of toxic organic pollutants.
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İncele - MakaleAçık Erişim2024Scopus SJRQ2Green synthesis of (S)-1-(furan-2-yl)propan-1-ol from asymmetric bioreduction of 1-(furan-2-yl)propan-1-one using whole-cell of Lactobacillus paracasei BD101
Bülbül, Ali Savaş; Şahin, Engin
Chiral heterocyclic alcohols are important precursors for production of pharmaceutical medicines and natural products. (S)-1-(furan-2-yl)propan-1-ol ((S)-2) can be used production of pyranone, which can be used in the synthesis of sugar analogues, antibiotics, tirantamycines, and anticancer drugs. The synthetic approaches for (S)-2, however, have substantial difficulties in terms of inadequate enantiomeric excess (ee) and gram scale synthesis. Moreover, the biocatalytic synthesis of (S)-2 is unknown until now. In this study, the synthesis of (S)-2 was carried out by performing the asymmetric bioreduction of 1-(furan-2-yl)propan-1-one (1) using the Lactobacillus paracasei BD101 biocatalyst obtained from boza, a grain-based fermented beverage. (S)-2 was obtained with >99% conversion, >99% ee, and 96% yield under the optimized conditions. Furthermore, in 50 h, 8.37 g of 1 was entirely transformed into (S)-2 on gram scale (96% isolated yield, 8.11 g). This is the first report on the high-gram scale biocatalyzed synthesis of enantiopure (S)-2. These data suggest that L. paracasei BD101 can be used to bioreduction of 1 in gram scale and efficiently produce (S)-2. Furthermore, these findings laid the base for future study into the biocatalytic production of (S)-2. It was particularly notable as it was the highest known to date optical purity of (S)-2 generated by asymmetric reduction using a biocatalyst. This work offers a productive environmentally friendly method for producing (S)-2 using biocatalysts.
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İncele - MakaleAçık Erişim2023Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities
Bülbül, Ali Savaş; Sen, Fatih; Bekmezci, Muhammed; Karakaya, Funda
In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and UV-vis spectroscopy (UV-VIS). According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10⁻³ g/mL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.
AtıfOpenAlex1TR Dizin1İncele - MakaleAçık Erişim2023Alyssum filiforme Bitkisinin Antimikrobiyal, Antibiyofilm, Antioksidan Aktivitesinin ve Fenolik Profilinin Belirlenmesi
Kara, Yakup; Bülbül, Ali Savaş; Orçan, İlayda
Öz − Bakteriler antibiyotiklere ve türevlerine karşı günden güne direnç kazanmaktadır ve bu küresel bir sorun haline gelmektedir. Bitkilerde bulunan sekonder metabolitlerin (fenolik bileşikler, flavonoidler, alkoloidler gibi) antioksidan ve antimikrobiyal gibi çeşitli aktiviteleri sebebiyle bu küresel sorunun önüne geçilebileceği düşünülmektedir. Bu kapsamda çeşitli çalışmalar yapılmış ve halen de yapılmaktadır. Bitkisel kaynaklardan antioksidan, antimikrobiyal ve anti-biyofilm gibi etki gösteren bileşenlerin belirlenmesi ile antibiyotik ve türevlerine karşı oluşan direnç aşılabilir. B vitamini ve magnezyum açısından zengin olan Brassicacea ailesinin bir üyesi de Alyssum filiforme türüdür. Alyssum filiforme bu kapsamda değerlendirilebilecek potansiyele sahip bir endemik türdür. Bu çalışmada; endemik olarak yetişen türün antimikrobiyal, anti-biyofilm, antioksidan ve RP-HPLC-PDA ile fenolik profili belirlendi. Elde edilen ekstre antimikrobiyal aktivite için Minimum İnhibisyon Konsantrasyonu (MIC) ve Minimum Bakterisidal Konsantrasyonu (MBC) yöntemleri uygulandı, antioksidan aktiviteyi belirlemek için ise 2,2-difenil-1-pikrilhidrazil (DPPH) radikalini temizleme aktivitesi ve demir indirgeme antioksidan güç (FRAP) metodları kullanıldı. Biyofilm oluşumunu önlemedeki etkinliği gözlemleyebilmek için kristal viyole bağlama yöntemi kullanıldı. Tüm bakterilerde olumlu sonuçlar elde edildi. FRAP ve DPPH sonuçları sırasıyla 13.070 μmol FeSO4.7H2O/g ve SC50 5.190 mg/ml olarak belirlendi. Elde edilen fenolik analiz sonucunda bitkinin kafeik asit ve krisin yönünden zengin olduğu bulundu. Elde edilen sonuçlar ışığında Alyssum filiforme türünün sağlık açısından değerli bir tür olduğu düşünülmektedir.
AtıfOpenAlex3TR Dizin1İncele - MakaleAçık Erişim2023Antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of methanol extracts of some acantholimon boiss (plumbaginaceae) species
Bülbül, Ali Savaş; Sen, Fatih; Bostancı, Maşide Temiz; Uygun, Cihangir; Bekmezci, Muhammed; Bayat, Ramazan
Therapeutic properties of plants have been used since the existence of mankind. To benefit from this feature, the discovery of the active components of plants has gained momentum today. One of these plant species is Acantholimon Boiss. This plant extract has antifungal, antimicrobial, and anticancer properties. In this article, antimicrobial, antifungal and antioxidant capacities and antibiofilm properties of Acantholimon Boiss plant extract on 15 bacterial and 2 fungal species were evaluated. Antimicrobial properties were evaluated by Agar Well Diffusion method. It was observed that the plants formed a zone diameter of 12-18 cm. Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were used to determine the minimum active property. Biofilms of almost all bacteria were reduced by 10-50% by Acantholimon species at doses of 8 mg/ml-16 mg/ml. For MCF-7 cells, the IC50 values for A. acerosum subsp. brachystachyum (Boiss. ), A. acenum var. balansae, A. armenum var. balansae and A. kotschyi were 0.143 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml and 0.107 mg/ml, respectively. The same doses did not show a cytotoxic effect on HUVEC cell lines. With this article, it has given important results in terms of having a wide range of bacterial studies and examining its activities in fungi and cell lines. These findings suggest that extracts of Acantholimon Boiss species may be a potential source of natural medicines and antibacterial, antifungal, antibiofilm, antioxidant, and anticancer activities may support their therapeutic use.
- MakaleAçık Erişim2023Scopus SJRQ2Efficient bio-catalytic production of enentiopure (S)-(4-chlorophenyl)(phenyl)methanol as a drug precursor by using a novel rotatable composite design-based optimization strategy
Tozlu, Nesrullah; Bülbül, Ali Savaş; Özdemir, Akın; Şahin, Engin
Asymmetric bioreductions catalyzed by biocatalysts have demonstrated great promise in manufacturing chiral alcohols. On the other hand, the synthesis of (S)-(4-chlorophenyl)(phenyl)methanol ((S)-2), precursors of L-cloprastine and carbinoxamine, still presents considerable challenges due to the inadequate substrate quantity and production process. In this work, a novel rotatable composite design-based optimization technique was used with the Lactobacillus paracasei BD101 biocatalyst for the asymmetric reduction of (4-chlorophenyl)(phenyl)methanone (1). Optimization conditions of the reaction were determined by the proposed optimization strategy as: pH=5.85, temperature=37 °C, incubation time=71 h, and agitation speed=120 rpm. The reaction conversion and the product of enantiomeric excess (ee) were also predicted to be 97% and 99%, respectively. In the experimental study performed under the determined optimized conditions, (S)-2 was obtained with >99% ee, >99% conversion, and 97% yield. In addition, 1 with the amount of 15.166 g was completely converted to (S)-2 (14.85 g, 97% isolated yield) on a high-gram scale. Notice that the manufacture of (S)-2 on a gram scale utilizing a biocatalyst and an optimization technique is demonstrated in this work for the first time. Finally, an economical, effective, and environmentally friendly biocatalytic process for the biocatalytic synthesis of (S)-2, which have antitussive and antiemetic properties and relax the bronchial muscle, has been demonstrated by the novel rotatable composite design-based optimization method.
AtıfScopus3WoS3OpenAlex2İncele - MakaleAçık Erişim2022Scopus SJRQ2Pollen morphology of some Veronica species (Plantaginaceae) from Turkey
Varlik, Kader; Armagan, Metin; Bülbül, Ali Savaş
The aim of the present work is to provide an account on the morphological features of pollen of Veronica species in Turkey, as a contribution to future research. Pollen morphology of 20 species of the Veronica were investigated using Light Microscope (LM) and Scanning Electron Microscope (SEM). Different palyno-morphological features including; pollen ornamentation, colpus ornamentation, size of polar and equatorial diameter, P/E ratio, exine thickness, and size of colpus were observed. The study revealed that the pollen grains of the studied Veronica species were in all cases tricolpate, radially symmetrical and isopolar, oblate-spheroidal or prolate spheroidal. The surface pattern of the exine varied from striato-reticulate and striatomicroreticulate to microechinate-perforate, which allows recognition of three homonymous types of pollen grains.
AtıfScopus1WoS1OpenAlex1İncele - MakaleAçık Erişim2022Scopus SJRQ3Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits
Şahin, Birgütay; Bülbül, Ali Savaş; Çelik, I. Seyfettin; Korkmaz, Nesrin; Karadağ, Ahmet
In this study, biological activities of silver nanoparticles (AgNP) synthesized by green synthesis method using the fruit extract of Gilaburu (Viburnum opulus L.) plant were investigated. The characterization of the synthesized AgNPs was performed by ultraviolet visible region spectroscopy (UV-Vis), Fourier transform - infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques. The crystal size was found to be 52.32 nm when the XRD data were calculated with the Debye-Scherrer's equation. The antimicrobial activities of extract and AgNPs were investigated by microdilution and disk diffusion methods. Antibiofilm activities were examined by the crystal violet technique. The cytotoxic effects of the extract and AgNPs against MCF-7 (human breast cancer) and HUVEC (human umbilical vein endothelial cells) cell lines were evaluated by MTT assay. The IC50 values for the HUVEC line were found to be 0.97 mg/mL (AgNP) and 85.24 mg/mL (extract), while the IC50 values for the MCF-7 line were determined as 0.022 µg/mL (AgNP) and 0.021 µg/mL (extract). To our knowledge, this is the first report to comprehensively examine the biological activities of V. opulus L. extract and biosynthetic AgNPs.
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