The In-vitro Effect of Lisonopril on Serum Alkaline Phosphatase and Acid Phosphatase Enzymes Activity

Hypertension is a disease that most elderly people suffer. People with high blood pressure often obliged to use the drug that lowers blood pressure in order to maintain a healthy lifestyle throughout their lives. In this study, the in vitro effect of lisonopril on serum alkaline phosphatase and acid phosphatase enzymes which are essential for normal bone formation and mineralization activities was investigated. Alkaline phosphatase enzyme was purified using DEAE-cellulose, acid phosphatase enzyme was purified using CM-cellulose ion exchange chromatography from human serum. The activities of alkaline and acid phosphatase were determined using pnitrophenylphosphate as the substrate. When finding out the activity of TrACP enzyme, adding tartrate to the environment provided inhibition of other acid phosphatase enzymes than TrACP. The activities of alkaline phosphatase and acid phosphatase, which is resistant to tartrate, have been found at five different concentrations of substrates (0.033, 0.1, 0.16, 0.23 and 0.3 mm) in environment with inhibitor and without inhibitor. While three different concentrations of the inhibitor, including 10-2,10-3 and 10-4, was used for each substrate concentration. It was found that the drug inhibit both enzymes, and I50 value was calculated as 0.17 mm and 0.79 mm. Furthermore, Ki values were calculated as 6.98x10-4, 7.06x10-4 for ALP and TrACP respectively by utilizing the Lineweaver-Burk plot