Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Can, Zehra(5)
- Kara, Yakup(4)
- Ecem Bayram, Nesrin(3)
- Gerçek, Yusuf Can(3)
- Kolaylı, Sevgi(3)
- Baştürk, Fatma Nur(2)
- Cevahir Öz, Gül(2)
- Kolayli, Sevgi(2)
- Çelik, Saffet(2)
- Acar Şahin, Aydan(1)
- Asadov, Elsevar(1)
- Bayram, Sinan(1)
- Birinci, Ceren(1)
- Boyraci, Gulsum Merve(1)
- Degirmenci, Atiye(1)
- Demir Kanbur, Esra(1)
- Esertaş, Ülkü Zeynep Üreyen(1)
- Gıdık, Betül(1)
- Kemal, Mehmet(1)
- Kutlu, Naciye(1)
- Ozkok, Aslı(1)
- Ozmert Ergin, Sema(1)
- Pınar, Nur Münevver(1)
- Sefalı, Abdurrahman(1)
- Yildiz, Oktay(1)
- Çevik, Berke(1)
- Özkök, Aslı(1)
- Özmener, Bahar(1)
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ1Deep Eutectic Solvents as Sustainable Media for Efficient Recovery of Phenolics From Black (Lycium ruthenicum Murr.) and Red (Lycium barbarum Lam.) Goji Berries: Optimization and Functional Evaluation
Gerçek, Yusuf Can; Kutlu, Naciye; Çelik, Saffet; Bayram, Sinan; Çevik, Berke; Baştürk, Fatma Nur; Özmener, Bahar; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have been frequently preferred over the past decade as alternatives to conventional solvents for the extraction of bioactive compounds from natural products. Meanwhile, black and red goji berries are widely consumed as functional foods due to their many functional properties reported in the literature. The aim of this study is to evaluate the effectiveness of different DESs in the extraction of individual phenolic compounds from goji berry (Lycium barbarum and L. ruthenicum) fruits, to determine the most suitable DES, and to optimize the extraction conditions. The DES that provided the highest yield in the extraction of individual phenolic compounds from L. barbarum was determined to be proline:citric acid (DES6 – a total of approximately 33,545 µg/kg), while for L. ruthenicum it was choline chloride:acetic acid (DES3 – a total of approximately 37,496 µg/kg). Rutin was the predominant phenolic compound in both species. DESs provided a higher phenolic yield compared to ethanol and water. In addition, L. ruthenicum exhibited higher total phenolics, flavonoids, proanthocyanidins, and antioxidant activity compared to L. barbarum. These findings reveal that DESs are a green, efficient, and sustainable alternative for the extraction of phenolic compounds from goji berries.
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İncele - MakaleAçık Erişim2025Scopus SJRQ2Quantification of phenolics by LC-MS/MS of monofloral and polyfloral Honey: Evaluation of the greenness of the method with MoGAPI and MoGSA
Gerçek, Yusuf Can; Baştürk, Fatma Nur; Özkök, Aslı; Cevahir Öz, Gül; Ecem Bayram, Nesrin
This study evaluated the physicochemical properties, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, proanthocyanidin content, and polyphenolic profiles of monofloral and multifloral honey samples produced in Türkiye. In addition, the greenness of the analytical method was assessed using two different evaluation tools: MoGSA and MoGAPI. The TPC and TFC values for multifloral honeys ranged from 0.18–0.65 mg GAE/g and 0.01–0.09 mg QE/g, respectively, whereas for monofloral honeys, these values ranged from 0.16–0.98 mg GAE/g and 0.01–0.37 mg QE/g. The highest CUPRAC and DPPH values were observed in Erica spp. honey, consistent with its TFC and total individual polyphenolic compound results. Notably, the Erica spp. honey sample exhibited a significantly higher gallic acid content (26.13 µg/g) compared to the other samples. These findings suggest that Erica spp. honey characterized by its high TPC, antioxidant activity, and distinctive polyphenolic profile, may serve as a notable dietary source of antioxidants. Additionally, the MoGAPI and MoGSA scores of the method were determined to be 84 and 91.67, respectively, demonstrating its high environmental sustainability.
AtıfScopus4WoS4OpenAlex3İncele - MakaleAçık Erişim2024Scopus SJRQ1Determination of phenolic content and bioactive characterization of Anatolian propolis
Can, Zehra; Birinci, Ceren; Kara, Yakup; Esertaş, Ülkü Zeynep Üreyen; Kolaylı, Sevgi
In this study, propolis referred to as of Türkiye Anatolian propolis was utilized. This work was aim to prepare a 70% ethanolic extract of propolis and subsequently determine certain biochemical parameters, total phenolic content (TPC), total flavonoid content (TFC), phenolic and flavonoid composition, inhibitory effects on certain enzymes [acetylcholinesterase (AChE), urease, and alpha-amylase], along with assessments of antiviral activity against Vero cells, lung cancer cells (A549), and breast cancer cells (MDA-MB-231). The richness of phenolic content in Anatolian propolis clearly indicates a high inhibitory effect on these enzymes. Additionally, it was observed to exert significant effects on cancer cells and demonstrated effective antiviral activity. It can be contemplated that the Anatolian propolis might serve as a potential source for novel drugs, and the isolation of its compounds could offer opportunities for utilization in traditional medicine.
AtıfScopus9WoS10OpenAlex11İncele - MakaleYalnız künye2024Scopus SJRQ3Physicochemical Characteristics, Phenolic Components, and Antioxidant Capacities of Lavender Honey (Lavandula Spp.) from Isparta Region of Türkiye
Kolayli, Sevgi; Can, Zehra; Kara, Yakup; Ozkok, Aslı; Ozmert Ergin, Sema; Kemal, Mehmet; Demir Kanbur, Esra
Lavender honey is one of the most desirable blossom honeys for its unique taste and aromatic qualities and can vary significantly from one region to another. In this study, melissopalynological, physicochemical and antioxidant properties of lavender honey from the Isparta region of Türkiye were investigated. The ration of the Lavandula spp. pollen were identified ranging from 9 % to 76 % in the thirteen samples. The average pH, conductivity, moisture, Hunter Lab* and proline values were 3.72, 0.22 mS/cm, 17.17 %, L*: 66.56, a*: 66.32, b*:18.41 and 576.92 mg/kg, respectively. The average F+G and F/G were 67.66 % and 1.02, respectively. The average of total phenolic (TP) and flavonoid (TF) substance were found to be 39.40 mg GAE/100 g and 3.23 mg QUE/100 g, respectively. The average total antioxidant capacity (FRAP) and DPPH radical scavenging activity values were found to be 178.28 μmol FeSO4 ⋅ 7H2O/100 g and 53.09 mg/mL, respectively. In the phenolic component analysis performed with HPLC-PDA, p-OH-benzoic acid, p-coumaric acid, chrysin and pinocembrin were detected as common polyphenols in the samples. Conclusively, despite considerable variability in their monofloral characteristics, no significant differences were observed in the physicochemical and antioxidant properties of lavender honeys.
AtıfScopus18WoS16OpenAlex18İncele - MakaleAçık Erişim2022Scopus SJRQ2What Should Be the Ideal Solvent Percentage and Solvent-Propolis ratio in the Preparation of Ethanolic Propolis Extract?
Kara, Yakup; Can, Zehra; Kolaylı, Sevgi
The biological activity of propolis is mostly due to its polyphenols. Raw propolis is consumed after being extracted in various solvents, ethanol being the most commonly used. However, the ideal percentage and ratio of ethanol for use in the preparation of propolis extracts are uncertain. The aim of this study is to compare the phenolic compositions and antioxidant activities of propolis extracts extracted with different ethanol concentrations and extraction conditions. Two different extraction methods were used, normal and ultrasonic extraction. The propolis extracts were evaluated in terms of total phenolic content, total flavonoid content, ferric reducing/antioxidant power,1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, and phenolic compositions using RP-HPLC–PDA. The propolis extracts with the highest activity were determined beforehand by preparing ethanol–water mixtures at different percentages, ranging from 0 to 99.9%. The most suitable propolis-to-solvent ratio was then determined. The optimum ethanolic percentage varied between 60 and 70%, the ultrasonic extraction process increased the efficiency, and the ideal solvent-propolis ratio (w/v) ranged between 1:5 and 1:10.
AtıfScopus39WoS33İncele - MakaleAçık Erişim2022Scopus SJRQ2Anatomy, trichome micromorphology and phytochemical profile of Stachys rizeensis R.Bhattacharjee from Turkey
Gerçek, Yusuf Can; Acar Şahin, Aydan; Ecem Bayram, Nesrin; Çelik, Saffet; Sefalı, Abdurrahman; Gıdık, Betül; Cevahir Öz, Gül; Pınar, Nur Münevver
In recent years, the popularity of natural products and natural product formulations has increased due to their beneficial effects on health. As a part of this trend, Lamiaceae family members have attracted attention due to their rich bioactive properties. Although the Stachys rizeensis R.Bhattacharjee plant is an endemic plant species in Turkey, no studies focus on its characterization. Hence, the present study aims to demonstrate the anatomical, morphological and phytochemical characterization of S. rizeensis in Turkey. In the first stage of the study, anatomical and trichome morphological characterization was performed to contribute to the taxonomic definition of S. rizeensis was found to be anatomically different from some other previously studied Stachys sp. by showing lacunar collenchyma type, uncertain cambium at the corners, two or three rows of endodermis in certain areas in the stem and two rows of palisade parenchyma, capitate and peltate glandular trichomes in the leaf. In the second stage, the phenolic profile, total phenolic/flavonoid content and antioxidant activity of the plant extracts prepared using solvents with different polarities (ethanol, methanol, water, ethyl acetate and hexane) were tested to determine the phytochemical content of the plant. The phytochemical analysis supported by chemometric analysis (PCA and HCA) revealed that methanol was the most efficient organic solvent for the extraction of the phytochemical compounds of the plant. Additionally, it was observed that chlorogenic acid (1842.40–217,086.19 mg/kg), verbascoside (2591.43–204,429.81 mg/kg), luteolin (3579.56–88,636.14 mg/kg) and kaempferol (9706.30–50,340.93 mg/kg) compounds were present at maximum levels in this plant, in which 31 different phenolic compounds were scanned using LC-MS/MS. In addition, results obtained from the phytochemical analysis revealed that S. rizeensis is a valuable plant that can be used for medical protection/treatment and that it could also have a variety of culinary applications. The present study served as the first original report regarding the chemical profile and anatomical characteristics of S. rizeensis (Lamiaceae) from Turkey.
AtıfScopus4WoS4OpenAlex4İncele - MakaleAçık Erişim2022Scopus SJRQ2Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product
Kara, Yakup; Can, Zehra; Kolaylı, Sevgi
In this work, a reverse phase high-performance liquid chromatographic method (RP-HPLC) with photo diode array (PDA) at 4 different wavelengths (250, 280, 320 and 360) for the determination of some polyphenols in some bee products (honey, pollen, and propolis) is developed. The analyses were carried out on C18 column (250 mm x 4.6 mm, 5 μm; GL Sciences), and the gradient program was used with a mobile phase A reservoir with 70% acetonitrile and the B reservoir with 2% acetic acid. The HPLC method was founded that limit of detection was in the range 0.022-0.0908 μg/mL; the limit of quantification was in the range 0.074-0.3027 μg/mL, all calibration curves were linear R2>0.995 within the range, the recovery range was 91.43-111.37% for 10 ppm and 98.44-101.68% for 40 ppm and relative error levels 0.0330-0.0290 respectively. The developed method was applied to some bee products available on the Turkey market. The study aimed at phenolic profiles of the bee products extracts were revealed by using 25 phenolic standards. The proposed method was optimized, quickly, and simple validated by evaluating the linear range, the limits of detection and quantification, the accuracy, the precious, the repeatability, and recoveries suitable for the phenolic analysis. It is concluded that evaluated and the quantitative determination of the bee products can be made quickly and reliably with the optimized method.
AtıfScopus35WoS35OpenAlex41İncele - MakaleYalnız künye2020Scopus SJRQ2Honeys from some different regions of Azerbaijan: bioactive characteristics based on phenolic profile and antioxidant activity
Degirmenci, Atiye; Can, Zehra; Boyraci, Gulsum Merve; Yildiz, Oktay; Asadov, Elsevar; Kolayli, Sevgi
Azerbaijan is an important region for the beekeeping and honey industry. Biochemical characteristics of eleven honey samples from different regions of Azerbaijan were analyzed through measuring electrical conductivity, optical activity, moisture content, proline and diastase number. Phenolic components are analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC) with UV–Vis double beam detector. Sugars profiles of samples were carried out using HPLC with a refractive index detector (RID). Total phenolic content (TPC), ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity were measured with the aim of gathering information on the antioxidant power of samples. Biochemical and antioxidant characteristics are also supported with melissopalynological data. Conductivity ranged between 0.32 and 0.82 mS cm−1, moisture 14.52 and 20.78%, proline 314.34 and 1077.21 mg kg−1, diastase 13.05 and 22.19. Azerbaijan honeys exhibited levorotatory properties. Total phenolic content varied from 18.82 to 154.75 mg gallic acid equivalent per 100 g honey. The results confirm that physico-chemical and biologically active properties of Azerbaijan honeys are affected by the flora and geographical variations.
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