Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ3Chemical Profiling of Stachys cretica subsp. anatolica Rech. f. (Endemic) Essential Oils and their Methanol Extracts with Evaluation as Enzyme Inhibitors, Antioxidant, and Antimicrobial Agents
Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat
Enzyme inhibition activities, phenolic compounds, antioxidant activities, bioactive compounds, antimicrobial activities, and chemical components of essential oil and methanol extracts obtained from the aerial parts of S. cretica subsp. anatolica were investigated. The main phenolic compounds of aerial parts were catechin, oleuropein, and epicatechin. The determined enzyme inhibitor activities highlight the potential of S. cretica subsp. anatolica as a source of bioactive compounds, particularly for carbonic anhydrase and cholinesterase inhibition. The essential oil and methanol extract exhibited remarkable activities against CA-II, AChE, and BChE, although they were less potent than standard inhibitors. The essential oils generally showed stronger antimicrobial activity compared to the 30% methanol extracts across most bacterial and fungal strains, as evidenced by minimum lethal concentration (MLC) and lower minimum inhibitory concentration (MIC) values and larger inhibition zones. Chloramphenicol used alone exhibited the highest antimicrobial efficacy, with the lowest MIC and MLC values and the largest inhibition zones. The essential oils of S. cretica subsp. anatolica were determined as esters, oxygenated sesquiterpenes, and aldehydes in aerial parts. The main components were found to be hexahydrofarnesyl acetone in the aerial parts.
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İncele - MakaleAçık Erişim2026Scopus SJRQ3Chemical Profiling and Biological Activities of Methanol Extracts and Essential Oils From Ferulago Platycarpa Boiss. & Balansa (Endemic): Enzyme Inhibition, Antioxidant, and Antimicrobial Properties
Aygül, İmdat; Öz, Mehmet; Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman
This study aimed to evaluate the phytochemical composition and biological activities of Ferulago platycarpa methanol extract (ME) and essential oil (EO). The EO was obtained via hydrodistillation and analyzed by GC-MS, revealing sesquiterpene hydrocarbons as the dominant class, with caryophyllene (65.8%) and α-pinene (9.65%) as the major constituents. The methanol extract was subjected to LC-MS/MS analysis, which identified eleven phenolic compounds, with chlorogenic acid as the most abundant. The extract showed significantly higher total phenolic (1196.22±11.64 mg GAE/100g) and antioxidant (1870.00±17.69 mg QEE/100g) contents compared to the ME. In vitro enzyme inhibition assays demonstrated that the methanol extract exhibited potent inhibitory activity against carbonic anhydrase II (CA-II, ICSO= 0.023 pg/mL), acetylcholinesterase (AChE, ICSO= 110 pg/mL), and butyrylcholinesterase (BChE, IC50= 390 pg/mL). In contrast, the EO showed higher inhibition against α-amylase (IC50= 5920±10.45 pg/mL) and BChE (IC50= 1.32±0.65 pg/mL), while its α-glucosidase showed no inhibition. Antioxidant assays indicated superior activity for the methanol extract compared to the EO. Furthermore, antimicrobial testing revealed that the EO demonstrated broader and more effective antimicrobial action, exhibiting lower MIC and MBC values against several bacterial and fungal strains. Collectively, these results highlight F. platycarpa as a valuable source of bioactive compounds with promising applications in antidiabetic, neuroprotective, antioxidant, and antimicrobial therapies.
- MakaleYayıncıda açık erişim2025Scopus SJRQ2Primula longipes subsp. bayburtensis Sefali, Yapar & Demir (Primulaceae): A new taxon from north-eastern Anatolia, Türkiye
Sefalı, Abdurrahman; Yapar, Yakup; Demir, İbrahim; Yurtvermez, Bayram; Keser, Ali Murat
The new taxon, Primula longipes subsp. bayburtensis Sefali, Yapar & Demir, subsp. nov. (Primulaceae) is described and illustrated from Bayburt Province, Türkiye. It is morphologically assigned to Primula sect. Crystallophlomis based on its long scapes, lanceolate and denticulate leaf with long petiole and hairless habit, fruits longer than calyx and covered by farina on the scape. This new taxon is similar to Primula longipes and P. nivalis subsp. turkestanica; it can also be easily distinguished by its calyx divided ratio, thurm flowers (unwidened corolla tube shape and length) and capsules (length) futures.
- MakaleAçık Erişim2025Scopus SJRQ3Chemical composition of essential oils and comparative analysis of enzyme inhibitory, antioxidant and antimicrobial activities in essential oils of endemic Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian
Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat; Öz, Mehmet
In this study, essential oil from the aerial parts of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian was extracted via hydrodistillation using a Clevenger apparatus. GC-MS/FID analysis determined aldehydes (28.31%) as the dominant group, with eucalyptol (8.71%) as the main constituent. Antioxidant activities were exhibited using DPPH, ABTS, and FRAP assays, with the essential oil demonstrating relatively higher antioxidant properties. The essential oil also showed higher levels of bioactive components, including total antioxidant capacity (160.26 mg AA eq./100 mL), total phenolic content (21.57 mg GA eq./100 mL), and total flavonoid content (14.85 mg QE eq./100 mL). While G. sericea essential oil demonstrated some antioxidant activity, it was significantly lower compared to the reference standard ascorbic acid (AA). The antimicrobial results showed relatively higher antimicrobial activity compared to most bacterial and fungal strains, as evidenced by lower MIC and MBC values and larger inhibition zones. Nevertheless, chloramphenicol (CH) and nalidixic acid (NA) continually showed superior antimicrobial efficacy, with the lowest MIC and MBC values and the largest inhibition zones. The essential oil also demonstrated potent enzyme inhibitory activity, especially against CA-II, AChE, and BChE, with the lowest IC50 value observed for CA-II, highlighting its potential as a potent enzyme inhibitor. However, the essential oil exhibited comparatively greater inhibition against AChE, BChE and α-amylase but was less effective overall. These findings suggest that the G. sericea essential oil has important antioxidant potential and enzyme inhibitory activity, while the essential oil shows varying efficacy across different enzymes.
AtıfOpenAlex1İncele - MakaleAçık Erişim2025Scopus SJRQ3Phytochemical analysis and biological evaluation of essential oils and extracts from Heracleum pastinacifolium subsp. incanum (Boiss. & A.Huet) P.H.Davis, an endemic plant from Turkey
Yuca, Hafize; Sefalı, Abdurrahman; Aydın, Bilge; Karadayı, Mehmet; Gülşahin, Yusuf; Yazıcı, Ayşenur; Karakaya, Songül; Demirci, Betül; Çoban, Furkan; Özdemir, Erkan
Essential oil content of and phenolic compounds flower-fruit, root, and aerial parts of Heracleum pastinacifolium subsp. incanum were analysed by GC/MS and LC/MS methods, respectively. Antidiabetic, anticholinesterase, and antioxidant activities of flower-fruit, root, aerial parts methanol extracts were evaluated. Apiole (35.0%), myristicine (72.2%), and myristicine (15.1%) were found as major compounds of fruit-flower mixture, root, aerial part essential oils, respectively. Hesperidin was found the highest amount in aerial part and flower-fruit extracts with 8904.2621 ng/mL and 11558.3634 ng/mL values, respectively. Fruit-flower extract showed the highest activity against α-glucosidase (24%). Root extract demonstrating the highest activity (18%) against AChE enzyme. Flowers-fruits mixture methanol extract had a higher % inhibition value on ABTS·+ and DPPH•. Flowers-fruits mixture methanol extract was rich in total phenol, total tannin, and protein content. All the extracts were determined as genetoxically safe according to the results of Ames/Salmonella, Escherichia coli WP2 and Allium cepa assays.
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İncele - MakaleAçık Erişim2022Scopus SJRQ2Anatomy, trichome micromorphology and phytochemical profile of Stachys rizeensis R.Bhattacharjee from Turkey
Gerçek, Yusuf Can; Acar Şahin, Aydan; Ecem Bayram, Nesrin; Çelik, Saffet; Sefalı, Abdurrahman; Gıdık, Betül; Cevahir Öz, Gül; Pınar, Nur Münevver
In recent years, the popularity of natural products and natural product formulations has increased due to their beneficial effects on health. As a part of this trend, Lamiaceae family members have attracted attention due to their rich bioactive properties. Although the Stachys rizeensis R.Bhattacharjee plant is an endemic plant species in Turkey, no studies focus on its characterization. Hence, the present study aims to demonstrate the anatomical, morphological and phytochemical characterization of S. rizeensis in Turkey. In the first stage of the study, anatomical and trichome morphological characterization was performed to contribute to the taxonomic definition of S. rizeensis was found to be anatomically different from some other previously studied Stachys sp. by showing lacunar collenchyma type, uncertain cambium at the corners, two or three rows of endodermis in certain areas in the stem and two rows of palisade parenchyma, capitate and peltate glandular trichomes in the leaf. In the second stage, the phenolic profile, total phenolic/flavonoid content and antioxidant activity of the plant extracts prepared using solvents with different polarities (ethanol, methanol, water, ethyl acetate and hexane) were tested to determine the phytochemical content of the plant. The phytochemical analysis supported by chemometric analysis (PCA and HCA) revealed that methanol was the most efficient organic solvent for the extraction of the phytochemical compounds of the plant. Additionally, it was observed that chlorogenic acid (1842.40–217,086.19 mg/kg), verbascoside (2591.43–204,429.81 mg/kg), luteolin (3579.56–88,636.14 mg/kg) and kaempferol (9706.30–50,340.93 mg/kg) compounds were present at maximum levels in this plant, in which 31 different phenolic compounds were scanned using LC-MS/MS. In addition, results obtained from the phytochemical analysis revealed that S. rizeensis is a valuable plant that can be used for medical protection/treatment and that it could also have a variety of culinary applications. The present study served as the first original report regarding the chemical profile and anatomical characteristics of S. rizeensis (Lamiaceae) from Turkey.
AtıfScopus4WoS4OpenAlex4İncele - MakaleAçık Erişim2021Scopus SJRQ2Biological activity of Psephellus appendicigerus as a natural agent
Acet, Tuba; Özcan, Kadriye; Sefalı, Abdurrahman
Psephellus appendicigerus, is a plant endemic to Turkey, belonging to the Asteraceae family, which is traditionally used in folk medicine in Turkey. The aim of this study is to reveal for the first time the biological activities of this plant and evaluate its potential use as a natural product. For this reason, different extracts (ethanol, methanol, ethyl acetate, and hexane) of the plant’s aerial parts were used in determining the antioxidant (2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl assays), antimicrobial (disc diffusion and microdilution methods), and enzyme inhibition activity (calorimetric methods) related to diabetes (α-amylase and α-glucosidase). Moreover, the phenolic constituents were analysed by high performance liquid chromatography. Methanol extract of the plant exhibited significant biological activity compared to other extracts. This extract had the highest amount of chlorogenic acid as phenolic compound. In addition, all extracts showed inhibition against at least one test microorganism. According to the results, P. appendicigerus has the potential to be used as a natural antioxidant, antimicrobial and enzyme inhibitor.
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