Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Can, Zehra(7)
- Ecem Bayram, Nesrin(3)
- Gerçek, Yusuf Can(3)
- Kara, Yakup(3)
- Kutlu, Naciye(3)
- Şahin, Engin(3)
- Arslaner, Ayla(2)
- Bayram, Nesrin Ecem(2)
- Bayram, Sinan(2)
- Gıdık, Betül(2)
- Kalay, Erbay(2)
- Kolayli, Sevgi(2)
- Kolaylı, Sevgi(2)
- Ozkok, Aslı(2)
- Özçelik, Beraat(2)
- AKMEŞE, Osman(1)
- AYGÜL, İmdat(1)
- Abca, Tuğba Elbir(1)
- Akar, Zeynep(1)
- Akgül, Halil İbrahim(1)
- Akköse, Ahmet(1)
- Alyafei, Mohammed(1)
- Anlar Daldal, Pınar(1)
- Anlar, Pinar(1)
- Aslanhan, Özlem(1)
- Ay, Mizgin(1)
- BALTACI, Cemalettin(1)
- Basturk, Fatma Nur(1)
- Bayraktar, M. Kilic(1)
- Baştürk, Fatma Nur(1)
- Boztaş, Kadir(1)
- Cakir, O.(1)
- Cakir, Ozlem(1)
- Cavrar, Sevda(1)
- Cebeci, Fatma(1)
- Cetinkaya, Hatice(1)
- Choudhary, R.(1)
- Cinar Topcu, Kübra(1)
- Dagsuyu, Eda(1)
- Degirmenci, Atiye(1)
Yayınlar
- MakaleYalnız künye2026Scopus SJRQ2Hot injection synthesis of metal-doped Ag2S nanocrystals: A novel multifunctional approach for cancer therapy, antibacterial strategies, and antioxidant defense
ULUSU, Funda; OZEL, Sultan Suleyman; Sarilmaz, Adem; ULUSU, Yakup
This pioneering study presents the synthesis and comprehensive evaluation of bare and metal-doped Ag₂S nanocrystals (NCs) as multifunctional agents with potential applications in antibacterial, antioxidant, and anticancer therapies. The incorporation of manganese, nickel, cobalt, and zinc into Ag₂S NCs resulted in a notable enhancement of their bioactivity, representing a novel approach in nanomaterial design. The antibacterial assessments revealed that Mn:Ag₂S NCs were the most potent candidate, exhibiting exceptional activity against S. aureus and E. coli, with the lowest MIC values recorded at 1.28 mg/mL and 1.08 mg/mL, respectively. The antioxidant studies demonstrated that the Mn:Ag₂S NCs exhibited remarkable free radical scavenging and ferric reducing capacities, with the lowest IC50 values (22.69 µg/mL for DPPH and 61.31 µg/mL for FRAP) underscoring their potential to mitigate oxidative stress. Importantly, cytotoxicity assays revealed that Ni:Ag₂S and Mn:Ag₂S nanocrystals selectively inhibited the proliferation of human colon cancer cells (HT-29), achieving IC50 values of 61.51 µg/mL and 90.12 µg/mL, respectively. Furthermore, these NCs demonstrate reduced toxicity towards mouse fibroblast cells (L929). This selective cytotoxicity indicates the potential of these agents in targeted cancer therapies, thereby reducing damage to healthy tissues. To the best of our knowledge, this is the first study to systematically explore the synergistic effects of metal doping on the multifunctional properties of Ag₂S NCs. These findings provide a robust basis for further in vivo studies and offer new avenues for the development of safe and effective nanomaterials in biomedical applications, particularly as antimicrobial, antioxidant, and anticancer agents.
AtıfScopus1WoS1OpenAlex1
İncele - MakaleAçık Erişim2026Scopus SJRQ1Gastrointestinal stability and bioactivity of propolis from diverse origins
Can, Zehra; Çelebi, Zeynep Berin; Tunca, Erol; Kolaylı, Sevgi; Sevin, Sedat; Tutun, Hidayet
Propolis is a chemically complex bee product whose biological properties depend on its botanical and geographical origin. However, little is known about how gastrointestinal digestion affects its phenolic profile and bioactivity. In this study, twelve propolis samples from eight countries were evaluated for phenolic and flavonoid contents, antioxidant capacity (FRAP, ABTS+•), and urease inhibition before and after simulated gastric digestion. RP-HPLC–PDA identified 26 phenolic compounds showing substantial compositional diversity. Turkish Bolu propolis retained the highest post-gastric FRAP value, while several samples (BBP, AFP) maintained or enhanced urease inhibition. PCA and HCA highlighted origin-dependent differentiation, identifying digestive stability as a key variable for functional potential.
AtıfOpenAlex2İncele - MakaleAçık Erişim2026Scopus SJRQ1Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Polyphenols From Tea Parts: A Green Assessment
Gerçek, Yusuf Can; Baştürk, Fatma Nur; Gıdık, Betül; Abca, Tuğba Elbir; Yurtvermez, Bayram; Fuente-Ballesteros, Adrián; Kutlu, Naciye; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have emerged as green alternatives to conventional solvents for extracting bioactive compounds from plants. This study evaluated nine choline chloride–based DESs for recovering antioxidant polyphenols from four parts of the tea plant (Camellia sinensis): seed, bud, leaf, and flower. Total phenolic content, antioxidant activity, and individual polyphenolic profiles were determined, and the greenness of the sample preparation was assessed using AGREEprep. The highest total phenolic content (53.28 mg GAE/g) and antioxidant activity (141.17 mg TE/g) were obtained from leaves using ChCl:formic acid, outperforming ethanol and methanol. Leaves also exhibited the highest total quantified polyphenolic content (up to 95,813 mg/kg DW, corresponding to the sum of the quantified polyphenols), with epicatechin gallate and gallocatechin + epigallocatechin being the major contributors. Flowers and buds showed notable polyphenolic diversity, while seeds contained the lowest levels. Statistical analysis confirmed clear clustering patterns according to plant part and solvent type. AGREEprep evaluation yielded scores up to 0.54, highlighting the environmental benefits of DES-based extraction. We demonstrated that tea leaves were the richest source of bioactive polyphenols, and selected DESs, particularly formic acid–based and ethylene glycol–based, exhibited superior efficiency and sustainability compared to conventional solvents. These findings support the wider adoption of DESs for green, high-yield extraction of pharmacologically important compounds from tea and other medicinal plants.
- MakaleYayıncıda açık erişim2026Scopus SJRQ1Optimization of extraction conditions of Micromeria fruticosa and use of optimized extract in beef patties production
Anlar Daldal, Pınar; Cinar Topcu, Kübra; Çakır, Özlem
This study aimed to optimize the conditions for obtaining an ethanol extract from Micromeria fruticosa (M. fruticosa) and to determine the antioxidant activity and phenolic compound content of the resulting extract. Subsequently, the use of the optimized extract in beef patties was evaluated, and the effects of the cooking process on the physicochemical properties of the product were examined. In this context, the highest efficiency was obtained in terms of total phenolic contents (TPC), as well as DPPH (2,2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) values, during the ethanol extraction of the plant. The optimum extraction conditions were determined to be 12 g of sample, 20 min, and 75% ultrasonic power. Under these conditions, the TPC of the extract was found to be 37.128 mg GAE/g dry weight, the DPPH value was 42.865, and the FRAP value was 254.776 mg TE/g dry weight. In the second phase of the study, the optimized extract was used at different concentrations (0%, 0.5%, 1.0%, and 1.5%) to produce beef patties, which were then analyzed for pH, moisture content, TBARS (thiobarbituric acid-reactive substances), and cooking yield. The use of the extract did not cause a statistically significant difference in the moisture content and TBARS values of the samples (p > 0.05). However, it was determined that the pH values of the samples containing 0% and 0.5% extract were lower than those containing 1.0% and 1.5% extract (p < 0.01). In addition, it was observed that the use of the extract had a positive effect on cooking yield, and that cooking yield increased with increasing extract concentration. As a result of the study, it was concluded that M. fruticosa holds promising potential as a natural additive in meat and meat products, offering a functional ingredient that can contribute to the development of value-added, health-oriented food products.
- MakaleAçık Erişim2026Scopus SJRQ2Probiotic Yogurt With Chokeberry (Aronia melanocarpa (Michx.) Elliot) Sauce Flavored With Date (Phoenix dactylifera L.) Fruit: Comparison of Nero and Viking Cultivars
Arslaner, Ayla; Yücetepe, Aysun; Akköse, Ahmet
The present study investigated the effect of adding chokeberry sauce (CS) from different cultivars (Nero and Viking) on specific quality attributes, total phenolic contents, antioxidant activity, textural qualities, sugar profile, probiotic shelf life, and sensory properties of probiotic yogurts, which were assessed throughout 21 days of cold storage. The results revealed that CS decreased fat, hardness, adhesiveness, and gumminess while increasing ash, total sugar, dry matter, and total calories. From Day 1 to Day 14 of storage, the pH values of CS-added samples significantly decreased (p < 0.01), whereas those in the control remained unchanged (Ky: 0% CS). The springiness and cohesiveness values of the Ky and Vy (20% Viking-CS) samples were comparable. During storage, CS-added yogurt samples’ water-holding capacity (WHC) was significantly higher than that of the control (p < 0.01). Interestingly, yogurt samples showed a notable rise in viscosity up to 14 days of storage, after which they slightly reduced. Ny (20% Nero-CS) and Vy samples exhibited higher red-blue hues than Ky. Vy had greater Lactobacillus acidophilus La-5 survival rates (99.28%) than Ny (99.04%). CS-added samples exhibited significantly higher antioxidant activity, total phenolic content, and total monomeric anthocyanin content compared to Ky; Vy also had the highest values (p < 0.05). Vy showed relatively high scores regarding organoleptic properties, including appearance, taste, texture and consistency, fruit ratio, overall acceptability, and average score. These indicators could help develop a healthier, low-calorie probiotic CS-added yogurt that competes with natural fruit-added yogurt.
- MakaleAçık Erişim2025Scopus SJRQ2Enzyme inhibitory, antioxidant, antimicrobial activities, and phenolic profiles of the methanol extract of Gelasia sericea an endemic species
AYGÜL, İmdat; AKMEŞE, Osman; SEFALI, Abdurrahman; BALTACI, Cemalettin; ÖZ, Mehmet; FIDAN, Muhammed Said
This study aimed to investigate, for the first time, the phytochemical composition, antioxidant, antimicrobial, and enzyme inhibitory properties of the methanolic extract of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N.Kilian. The extract demonstrated a moderate yield (6.55 g/100 g dry plant material) and was found to be rich in phenolic acids, particularly ferulic acid (17182.97 μg g-1), followed by caffeic acid, protocatechuic acid, and p-coumaric acid. Antioxidant analyses revealed high total phenolic (388.86 mg GA eq./100 g), total flavonoid (74.03 mg QE eq./100 g), and total antioxidant capacity (503.33 mg AA eq./100 g). The extract exhibited remarkable ABTS radical scavenging activity (95.99%, IC50 = 37.59 mg ml-1) and considerable ferric-ion reducing power (974.78 mg FeSO4 eq./100 g). Antimicrobial assays indicated moderate inhibitory effects, with stronger activity against Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) and the yeast Candida albicans, whereas Gram-negative bacteria were less susceptible. The extract’s MIC values ranged between 0.20-3.25 mg ml-1, and MLC values between 0.41-6.50 mg ml-1, which were higher than those of standard antibiotics. Enzyme inhibition assays revealed moderate activity against carbonic anhydrase II (IC50 = 0.0136 µg ml-1) and weak inhibition of cholinesterases and α-glucosidase, while α-amylase inhibition was relatively more pronounced (IC50 = 13350.00 µg ml-1). Overall, these findings highlight G. sericea as a valuable natural source of phenolic acids and antioxidants, with promising potential for applications in functional foods, nutraceuticals, and phytopharmaceuticals. However, the relatively limited antimicrobial and enzyme inhibitory activities suggest the need for further studies on bioavailability, in vivo efficacy, and compound isolation to optimize its practical applications.
- MakaleYalnız künye2025Scopus SJRQ1Extraction of Bioactive Compounds from Celery Root Peels Using an Ultrasound-Assisted Method with Choline Chloride–Based Deep Eutectic Solvents
Kamiloğlu, Aybike; Kutlu, Naciye; Elbir Abca, Tuğba; Yılmaz, Özlem
The concepts of sustainable and green technology have come to the forefront due to problems such as increasing environmental pollution, depletion of resources, and uncontrolled increases in industrialization. Green chemistry aims to reduce or completely eliminate pollution in the environment. In this study, both the ultrasonic method, which is a form of green technology, and deep eutectic solvents, which are among green solvents, were used, and a perspective on creating economic value from a waste product (celery root peel) is presented. In this context, phytochemical compounds (total flavonoid content and antioxidant activities) were obtained from celery root peels by ultrasonic-assisted extraction using 10 different choline chloride–based deep eutectic solvents, and the results were compared with methanol, an organic solvent. As a result, according to the total flavonoid content, the most effective deep eutectic solvent was choline chloride to sucrose, while according to the antioxidant activity value, choline chloride to lactic acid was found to be effective. For all of the analyzed bioactive compounds, higher or equal yields were obtained with all deep eutectic solvent extracts compared to methanol, and deep eutectic solvents could be alternatives to organic solvents (such as methanol) that may have toxic effects on humans or the environment.
AtıfScopus9WoS9OpenAlex10İncele - MakaleYalnız künye2025Scopus SJRQ2Bee bread from Anatolia: its chemical composition, phenolic and aromatic profiles, and antioxidant properties
Kolayli, Sevgi; Okumus Yukunc, Gulsah; Kara, Yakup; Demir Kanbur, Esra; Can, Zehra; Ozkok, Aslı; Tanugur Samanci, Aslı Elif
This study investigated the botanical origin, chemical composition, phenolic and volatile aromatic profiles, and antioxidant activities of 11 bee bread samples from different regions of Anatolia, Turkey. The bee bread samples contained high amounts of proteins (19.61 g/100 g) and lipids (6.43 g/100 g). The bee breads were determined to have a rich mineral content. The antioxidant potential of the bee breads was predicted using total phenolic content (TPC), total flavonoid content (TFC), total condensed tannin (TCT) content, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The TPC of the bee breads ranged between 2.041 ± 0.170 and 3.224 ± 0.006 mg GAE/g, while the TFC ranged from 0.323 ± 0.004 to 1.903 ± 0.017 mg QE/g. The FRAP assay showed antioxidant activity ranging from 17.778 ± 0.207 to 49.752 ± 0.856 µmol FeSO4.7H2O/g, and the DPPH values were between 1.054 ± 0.009 and 4.366 ± 0.014 SC50 mg/mL. Using 25 standards, RP-HPLC-PDA quantified the composition of the phenolic compounds, attributed to their antioxidant activity. The highest concentrations were detected for t-cinnamic acid, rutin, and p-coumaric acid. One hundred nineteen volatile aromatic compounds were determined in the bee breads by solid-phase microextraction coupled with gas chromatography and mass spectrometry (SPME-GC-MS). The obtained values suggest that bee breads could serve as a potential source of nutrients and bioactive compounds for value-added food supplements and functional foods.
AtıfScopus12WoS13OpenAlex15İncele - MakaleAçık Erişim2025Scopus SJRQ1Optimization of Ethanol Extraction Conditions From Sun-Dried Apricot and Prediction of Antioxidant, Phenolic, and Flavonoid Contents and Their Effects on HCT116 Cells Using Artificial Neural Networks
Topcu, Kubra Cinar; Gueven, Sara Altun; Cakir, Ozlem; Anlar, Pinar; Durul, Melekber Sulusoglu; Ay, Mizgin; Alyafei, Mohammed; Hayat, Faisal; Ercisli, Sezai
This study aimed to optimize the ethanol extraction conditions of sun-dried apricot (Prunus armeniaca L.) and evaluate the antioxidant capacity, phenolic, and flavonoid contents of the obtained extracts. Response surface methodology (RSM) was applied to determine the optimal extraction conditions, which were identified as 60 degrees C temperature, 34% ultrasonic power, 46 min sonication time, and a 4 g/mL solid-liquid ratio. Under these conditions, the extract exhibited a total phenolic content (TPC) of 4.20 mg GAE/g, a total flavonoid content (TFC) of 7.09 mg QE/g, a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of 1.37 mg TE/g, a ferric reducing antioxidant power (FRAP) value of 9.12 mg TE/g, and a thiobarbituric acid reactive substances (TBARS) level of 1.69 mg MDA/g. The artificial neural network (ANN) model provided highly accurate predictions for these parameters. Additionally, cell culture experiments demonstrated that the extract exerted a dose-dependent cytotoxic effect on HCT116 colon cancer cells, significantly reducing their viability. These findings highlight the potential of sun-dried apricot extracts as natural antioxidants with possible applications in the functional food and pharmaceutical industries.
AtıfScopus2WoS2OpenAlex2
İncele - MakaleYayıncıda açık erişim2024Scopus SJRQ1Enzyme Inhibitory, Physicochemical, and Phytochemical Properties and Botanical Sources of Honey, Bee Pollen, Bee Bread, and Propolis Obtained from the Same Apiary
Gercek, Yusuf Can; Dagsuyu, Eda; Basturk, Fatma Nur; Kırkıncı, Seran; Yıldırım, Nazlıcan; Kıskanç, Gamze; Özmener, Bahar; Unlu, Yigit Sabri; Kalkan, Seda Nur; Boztaş, Kadir
Bee products are an important source of nutrients and bioactive phytochemicals. This study aimed to determine the chemical composition (proximate composition, general phytochemical composition, sugar, and phenolic profiles) of four different products (honey, bee pollen, bee bread, and propolis), obtained from the same apiary, as well as to assess their biological activity through antioxidant and enzyme inhibition assays (α-amylase, α-glucosidase, lipase, AchE, neuraminidase, angiotensin-converting enzyme, urease, trypsin, tyrosinase, carbonic anhydrase, thioredoxin reductase, adenosine deaminase). Clear differences were observed among the samples in terms of both chemical composition and biological activity. The analysis revealed that bee pollen exhibited the highest carbohydrate content (87.9%), while propolis was identified as the richest source of phenolic compounds (14,858.9 mg/kg) among the analyzed samples. Propolis exhibited the highest biological activity in all applied antioxidant assays (CUPRAC, DPPH•, and ABTS•+) and in most enzyme inhibition assays. Notably, the α-glucosidase inhibition activity of propolis was comparable to that of the reference standard. In addition, honey exhibited remarkable trypsin inhibition, also comparable to the applied standard. These findings highlight the diverse bioactivities of hive products, which could play a key role in promoting health and preventing diseases.
AtıfScopus17WoS16OpenAlex16İncele - MakaleAçık Erişim2024Scopus SJRQ1Antioxidant activity and phenolic content of bee breads from different regions of Türkiye by chemometric analysis (PCA and HCA)
Can, Zehra; Gıdık, Betül; Kara, Yakup; Kolaylı, Sevgi
The objective of this study was to assess the antioxidant activity and phenolic compounds of bee bread samples obtained from various regions in Türkiye The goal was to characterize and classify 15 of bee bread samples based on their geographical origins. This investigation employed chemometric techniques, specifically principal component analysis (PCA) and a hierarchical clustering algorithm (HCA), for the inaugural comprehensive analysis of all data encompassing antioxidant activity and phenolic compounds in bee bread. The primary objective was to unveil potential clustering patterns among of bee bread samples based on their geographical origins. According to the results, the Total Phenolic Content (TPC) of bee bread samples ranged from 4.393 to 14.917 mg GAE/g dw, while the Total Flavonoid Content (TFC) exhibited variation within the range of 0.681 to 3.504 mg QE/g dw. p-OH benzoic and p-coumaric acids were detected in all samples. Other phenolic compounds were identified in different proportions among the bee bread samples. This study demonstrated the successful application of the PCA chemometric method to assess the antioxidant activity and phenolic compounds of bee bread samples from various regions of Türkiye. The results revealed effective clustering of the bee bread samples based on their geographical origins. Graphical Abstract: (Figure presented.)
AtıfScopus12WoS14OpenAlex16İncele - MakaleYalnız künye2023Scopus SJRQ2Design, synthesis, antioxidant and anticholinesterase activities of novel isonicotinic hydrazide-hydrazone derivatives
Aslanhan, Özlem; Kalay, Erbay; Tokalı, Feyzi Sinan; Can, Zehra; Şahin, Engin
The design and synthesis of hydrazone derivatives are increasing in popularity day by day due to the significant biological activities of this scaffold. In the present study, twelve novel isonicotinic hydrazide-hydrazone analogues were synthesized by the condensation reaction of isonicotinic hydrazide with benzaldehyde possessing sulfonate moiety. The structures of the novel compounds have been characterized in detail using spectroscopic techniques. All compounds have shown inhibitory effects against the AChE enzyme at rates ranging from 21.00 to 59.48%. Among them, compound 5 has exhibited the best inhibitory effect of 59.48% against AChE at a concentration of 0.1 mM. Furthermore, to determine how effective the novel compounds are as antioxidants, FRAP and DPPH studies were also carried out. FRAP values in compounds 1–12 were found to range from 26.989–3415.556 µmol FeSO4.7H2O/mg. They also displayed moderate antioxidant potential in the range of SC50=0.03–87.32 mg/mL compared with the control Trolox (SC50 =0.004) in DPPH radical scavenging activities. It was seen that the AChE inhibition percentages of the compounds were in the range of 23.04–58.10% at 0.1 mM concentration. This is the first research on the synthesis, antioxidant and enzyme inhibition properties of these compounds.
AtıfScopus43WoS37OpenAlex44İncele - MakaleYalnız künye2023Scopus SJRQ1Comparison of bioactive constituents by HPLC–DAD–ESI-MS and UFLC and in vitro antioxidant activities of blossom honey, bee pollen, and propolis
Saroğlu, Öznur; Ecem Bayram, Nesrin; Özçelik, Beraat
The aim of this study was to analyze the total phenolic content, phenolic profile, and antioxidant activity of honey, bee pollen, and propolis samples from Turkey's Black Sea Region. The total phenolic content of these bee products was found using Folin–Ciocalteu's method, and their antioxidant capacity was found using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cupric ion reducing antioxidant capacity (CUPRAC) assays. Phenolic compounds of honey samples were characterized by high-performance liquid chromatography coupled to a photodiode array detector and mass spectrometer (HPLC–DAD–ESI-MS), while phenolic compounds of bee pollen and propolis samples were quantified ultra-fast liquid chromatography (UFLC). The total phenolic values for honey, bee pollen and propolis were 6.32–18.21 mg GAE/100 g, 547.64–769.4 mg GAE/100 gr, and 6096.1–11,564 mg GAE/100 gr, respectively. DPPH and CUPRAC values of honey, pollen and propolis were 11.05–21.38% and 0.38–1.48 µmol Trolox/g; 24.67–38.63% and 0.03–0.05 mmol Trolox/g; 11.81–34.12% and 0.47–0.89 mmol Trolox/g, respectively. About 30 different phenolic compounds were identified as quantitative. Our findings have shown that all bee products examined in the study are found to contain gallic acid, naringenin, and caffeic acid. In terms of total phenolic content and antioxidant activity, the bee product extracts were ranked as follows: propolis > bee pollen > honey. The high concentration of phenolic compounds in propolis explains its remarkable antioxidant effect.
AtıfScopus10WoS10OpenAlex10İncele - MakaleAçık Erişim2023Scopus SJRQ3Synthesis of Arylsulfonyl Hydrazone Derivatives: Antioxidant Activity, Acetylcholinesterase Inhibition Properties, and Molecular Docking Study
Demirci, Yasin; Kalay, Erbay; Kara, Yakup; Güler, Halil İbrahim; Can, Zehra; Şahin, Engin
In this current paper, fifteen novel sulfonyl hydrazone derivatives have been successfully synthesized and evaluated for antioxidant activity as well as their effects on inhibitory activity toward acetylcholinesterase (AChE). By using 1H NMR, 13C NMR, FT-IR, and high-resolution mass spectrometry methods, the full characterization data of the novel compounds were obtained. The synthesized compounds capacity to inhibition glucosidase and exhibit antioxidant activity were tested in vitro. It was determined that compounds (E)-4-((2-((4-chlorophenyl)sulfonyl)hydrazone)methyl)-2-methoxyphenylfuran-2-carboxylate (21), (E)-2-methoxy-4-((2-(phenylsulfonyl)hydrazone)methyl)phenylfuran-2-carboxylate (17) and (E)-4-((2-((4-bromophenyl)sulfonyl)hydrazone)methyl)-2-methoxyphenylfuran-2-carboxylate (25) showed good antioxidant activity. Upon examining the acetylcholinesterase inhibitory activity, it was determined that compounds (E)-2-methoxy-4-((2-((4-methoxyphenyl)sulfonyl) hydrazone)methyl)phenylacetate (27) (10.39 μM), (E)-4-((2-((4-chloro phenyl)sulfonyl) hydrazone)methyl)-2-methoxyphenylfuran-2-carboxylate (21) (10.81 μM), (E)-4-((2-((4-chloro phenyl)sulfonyl)hydrazone)methyl)-2-methoxyphenyl thiophene-2-carboxylate (22) (12.92 μM) and (E)-2-methoxy-4-((2-(phenylsulfonyl)hydrazone)methyl)phenylfuran-2-carboxylate (17) (12.93 μM) showed potent inhibitory effects. Molecular docking simulations were used to investigate the interactions of novel sulfonyl hydrazone derivatives with human acetylcholinesterase protein. The ligands exhibited strong binding to the receptor protein with potent inhibition.
AtıfScopus18WoS18OpenAlex19İncele - MakaleAçık Erişim2023Scopus SJRQ3COMPARATIVE ANALYSIS OF BIOCHEMICAL CONTENT, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF HYPERICUM PERFORATUM L. SPECIES IS GROWN IN TÜRKİYE
Gul, Volkan; Cetinkaya, Hatice; Dinler, Burcu Seckin; Sefaoglu, Firat
Many external factors, such as climatic conditions, geographical differences, and altitude, directly affect the primary and secondary metabolites and therapeutic use of St. John's Wort (Hypericum perforatum L.), an important plant in alternative medicine. This study was carried out to identify the antioxidant and antimicrobial activities and the content of proline, malondialdehyde, hydrogen peroxide, flavonoid, anthocyanin, and total phenolic in the St. John's Wort growing naturally in different regions of Bayburt. The St. John's Wort samples were collected from 11 different locations. As a result of the analyses carried out on the plant samples, it was found that the content of proline was within the range of 4.6-8.2 µmol g-1 DW, the amount of malondialdehyde was within the range of 0.69-1.10 nmol g-1 DW, the amount of hydrogen peroxide was within the range of 79.89-155.49 µmol g-1 DW, the amount of flavonoids was within the range of 91.38%-100.27%, the amount of anthocyanin was within the range of 0.15-1.11 mkmol g-1 DW, the total phenolic content was within the range of 15.82-45.22 mg GAE g-1, and the antioxidant activity was within the range of 6.07-105.60 µg mL-1. In conclusion, although the biochemical contents and nonenzymatic antioxidant activities showed a wide variety, the antioxidant activity was found to be higher in samples 5, 8, and 11. According to the antimicrobial activity results, some plant extracts were effective on bacteria and fungi, especially samples 5, 6, 8, and 11 showed an effect at the concentration of 200 μg mL-1. This study, the first regional study on this subject, will contribute to future research and clinical trials in the health field.
AtıfScopus4WoS2OpenAlex3İncele - Yüksek Lisans TeziAçık Erişim2022İzonikotinik asitten türetilen yeni hidrazit-hidrazonların sentezi ve biyolojik aktivitelerinin değerlendirilmesi
Özlem Aslanhan
Hâlihazırda kullanılan antibiyotikler ve kemoterapötik ajanlar, artan bakteri direnci nedeniyle önümüzdeki yıllarda bulaşıcı hastalıkları başarılı bir şekilde tedavi etmek için yeterli olmayabilir. Bundan dolayı, önemli antibakteriyel ve antifungal aktiviteye sahip yeni bileşiklerin araştırılmasına ihtiyaç vardır. Hidrazid-hidrazon türevleri birçok biyoaktif molekülde bulunur ve antibakteriyel, antitüberküler, antifungal, antikanser, antienflamatuar, antikonvülsan, antiviral ve antiprotozoal etki gibi çok çeşitli biyolojik aktivite gösterirler. Bu bileşikler, geçmişten günümüze yeni ilaç geliştirme çalışmalarında incelenmektedir. Ülkemizin ilaç sektöründeki net ithalatçı konumu düşünüldüğünde okzadiazoller gibi ilaç olarak geliştirebilecek aktif maddelerin sentezlenmesinin ve incelenmesinin küresel pazardaki rekabet gücümüzü artırmak adına önemli olduğu düşünülmektedir. Bu tez kapsamında 12 tane sülfonat grubu içeren yeni hidrazid-hidrazon türevi sentezlendi ve karakterize edildi. Sentezlenen bu 12 yeni bileşiklerin FRAP ve DPPH yöntemine göre antioksidan aktiviteleri değerlendirildi. (E)-2-((2-izonikotinoilhidrazon)metil)fenil 4-metoksibenzensülfonat (3) bileşiği her iki yönteme göre en iyi antioksidan aktiviteye sahip olduğu görüldü. Elde edilen yeni bileşiklerin yapısında bulunan çeşitli grupların antioksidan aktivite üzerine etkili oldukları bulundu. Özellikle elektron verici grupların antioksidan aktiviteyi artırdığı belirlenmiştir. Elde edilen bu sonuçlara göre sentezlenen bu 12 yeni türev ilaç geliştirme çalışmalarında kullanılabilecek potansiyele sahip olduğu düşünülmektedir.
- MakaleYalnız künye2022Scopus SJRQ2Synthesis of 7-azaindole based carbohydrazides and 1,3,4-oxadiazoles; Antioxidant activity, α-glucosidase inhibition properties and docking study
Izgi, Samet; Sengul, Ibrahim F.; Şahin, Engin; Koca, Mehmet Serdar; Cebeci, Fatma; Kandemir, Hakan
In this current work, 7-azaindole based 1,3,4-oxadiazoles have been successfully prepared by treatment of 3-(hydrazonomethyl)-7-azaindole with the different acyl chlorides or acetic anhydrides to give the corresponding carbohydrazides, followed by iodine mediated synthetic protocol in order to afford the corresponding 2,5-disubstituted 1,3,4-oxadiazoles. The full characterization data of the novel compounds were obtained by utilizing 1H NMR, 13C NMR, FT-IR, high-resolution mass spectrometry and single crystal X-ray diffraction techniques. The antioxidant activity and α-glucosidase inhibition potential of the prepared compounds are examined by in vitro assays. The targeted hydrazide linked 7-azaindoles and their corresponding cyclized form 1,3,4-oxadiazoles exhibited inhibitory potential with IC50 values ranges between 0.46 and 24.92 mM. Plausible binding mode and interaction of ligands with α-glucosidase enzyme have been studied by molecular docking, supporting the experimental results.
AtıfScopus28WoS27İncele - MakaleYalnız künye2022Scopus SJRQ1Bioactive compounds of deep eutectic solvents extracts of Hypericum perforatum L.: Polyphenolic- organic acid profile by LC-MS/MS and pharmaceutical activity
Bayram, Sinan; Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) are known as environmentally-friendly solvents that have emerged as an alternative to conventional organic solvents in recent years. In this study, the effects of ten different DESs on the extraction of bioactive components from the Hypericum perforatum were investigated. Total phenolic content, total flavonoid content, phenolic profile (32 phenolics), organic acid profile (52 organic acids), as well as antioxidant and antimicrobial activities of H. perforatum extracts prepared using DESs and ultrasonic-assisted extraction method were determined. Total phenolic, total flavonoid content, and antioxidant activities of extracts were determined between 3.10 ± 0.86–16.64 ± 2.09 mg GA/g, 0.02 ± 0.00–12.29 ± 0.30 mg QE/g, and 8.40 ± 0.50–44.78 ± 2.66 mg TE/g, respectively. DES2 (choline chloride:ethylene glycol) and DES7 (choline chloride:lactic acid) demonstrated higher extraction success than other DESs for extracting phenolics and organic acids, respectively. DESs prepared using organic acid as hydrogen bond donor were found to have an antibacterial effect against all bacterial samples. However, the inhibitory effect against yeast-like fungi was observed in a narrower spectrum. Moreover, the results from principal component analysis also confirmed that the type of solvent used for extraction has a significant influence on the phenolics, organic acid, and other parameters. As a result, it was seen that DES components directly affect the extraction efficiency of target compounds from H. perforatum. This is a first and original report about use of DESs in extracting different bioactive components from H. perforatum.
AtıfScopus33WoS31OpenAlex32İncele - MakaleAçık Erişim2021Scopus SJRQ3Honey: Determination of volatile compounds, antioxidant and antibacterial activities
Karlidag, Semiramis; Keskin, Merve; Bayram, Sinan; Mayda, Nazlı; Ozkok, Aslı
Honey is an important functional food for human health and nutrition that is collected by honey bees and stored in the honeycombs. In this study, total phenolic content, antioxidant activity and volatile compounds of 13 different honey samples collected from various districts of Malatya province were investigated. As a result of this study, it was determined that the total phenolic content varied between 8.50 mg GAE 100 g–1 and 73.90 mg GAE 100 g–1 and it was observed that the honey samples were rich in aldehydes, aliphatic acid and esters, alcohols, hydrocarbons, carboxylic acid esters, ketones, terpenes, fatty acids and esters. In addition, the antibacterial effects of honey samples were determined against 18 different pathogenic bacteria using agar well diffusion (AWD) method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In AWD assay, it was recorded that inhibition zone diameters varied between 9 mm and 14 mm and honey samples were found to have a partial inhibitory effect against selected target pathogens.
AtıfScopus5WoS3OpenAlex7İncele - MakaleYalnız künye2021Scopus SJRQ1Orange, red and purple barberries: Effect of in-vitro digestion on antioxidants and ACE inhibitors
Şensu, Eda; Kasapoğlu, Kadriye Nur; Gültekin-Özgüven, Mine; Demircan, Evren; Arslaner, Ayla; Özçelik, Beraat
Bioactive compounds such as phenolics, anthocyanins and carotenoids were investigated in Berberis plant with purple (PB), red (RB) and orange (OB) fruits (barberries). The study was aimed to evaluate the bioaccessibility of the antioxidants and ACE (angiotensin converting enzyme) inhibitory compounds of barberry plants upon their transit through in-vitro digestion. Among barberries, (PB) exhibited the strongest antioxidant activity due to its higher phenolic and flavonoid content. The order of total phenolics and anthocyanins was PB > RB > OB. Chlorogenic acid was the main phenolic compound in all barberries even after in-vitro digestion. All barberries had similar amounts of total carotenoids (4.45 mgβ-carotene/100gDW). After in-vitro digestion, decreasing phenolics, anthocyanins and carotenoids led to a reduced antioxidant activity. Anthocyanins were found the most sensitive compounds in-vitro digestion. In terms of ACE inhibition activity, only RB exhibited ACE inhibition before (73.84%) and after (65.51%) in-vitro digestion. No ACE inhibition activity was detected at all in RB and OB samples. Leaves and branches of Berberis had also over 50% ACE inhibitory activity. To our knowledge, this is the first study on the carotenoids of barberry and the effect of in-vitro digestion on its bioactive compounds and ACE inhibitors.
AtıfScopus30WoS22OpenAlex26İncele