Sürdürülebilir Kalkınma Amaçları
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112 →- MakaleYalnız künye2026Evaluation of Phenotypic Traits and Essential Oil Content in Wild-Grown Achillea biebersteinii Populations in Northeastern Türkiye
Coban, Furkan; Gül, Volkan; Çakır, Ahmet
Achillea biebersteinii Afan., a medicinal and aromatic plant widely distributed in Türkiye, is valued for its essential oil content and bioactive compounds. This study aimed to investigate the phenotypic variation, yield performance, and essential oil content of A. biebersteinii populations collected from ten different locations in northeastern Türkiye. A total of six morphological and agronomic traits plant height (PH), inflorescence diameter (ID), stem diameter (SD), number of flowering branches (NFB), dry herbage yield (DHY), and dry flower yield (DFY) alongside essential oil content (EO), were evaluated. ANOVA results revealed statistically significant differences among locations for PH, DHY, and NFB, indicating strong environmental or genetic influences. In contrast, EO content showed no significant variation across locations, suggesting greater genetic stability. Correlation analysis indicated a strong positive association between NFB and DHY, while EO content was negatively correlated with PH and SD. Principal Component Analysis further clarified trait associations and revealed distinct groupings among locations, with L9 and L10 showing superior yield performance and L3 standing out for its high EO content. These findings highlight the substantial phenotypic and chemotypic diversity of A. biebersteinii in northeastern Türkiye and underscore the importance of location specific selection in breeding and conservation programs. The identification of high-yielding and high-quality populations provides a valuable resource for sustainable utilization of this species in medicinal and aromatic plant sectors.
- MakaleAçık Erişim2026Exploratory Proteomic Analysis of Rhododendron ponticum Bee Pollen Based on High-Resolution Mass Spectrometry
Ecem Bayram, Nesrin; Celik, Saffet
Bee pollen is a bee product that is widely preferred by people for its nutritional properties as well as its health-protective properties, and is defined as a ‘superfood’. In this preliminary exploratory study, monofloral Rhododendron ponticum bee pollen was analyzed using a label-free LC-QTOF-MS proteomic approach. According to the results obtained, a total of 37 proteins from the rhododendron plant have been fully confirmed in the pollen sample. Normalized spectrum counting values (NSAF) providing information about the semi-quantitative amount of these proteins were determined to be between 120.84 and 43259.22. Based on these findings, it can be said that the proteomic approach can provide biomarkers for monitoring the botanical source and quality of monofloral bee pollen. However, since no biological or technical replicate study was conducted, the findings obtained are exploratory in nature and should be considered semi-quantitative. To our knowledge, this study is the first to report the protein profile of R. ponticum bee pollen using a proteomic approach.
- MakaleAçık Erişim2026Phytochemical profile, antioxidant potential, and cytotoxic effect of the ethanol extract of Micromeria fruticosa
Çakır, Özlem; Topcu, Kübra Cinar; Anlar, Pinar
In this study, the ethanol extract of dried Micromeria fruticosa (L.) Druce, commercially sourced from the Tortum Valley (Erzurum Province), was comprehensively analysed to evaluate its bioactive potential. Considering that geographical origin and botanical differences significantly influence phytochemical composition and biological activity, the use of locally sourced commercial samples provides valuable data for plant standardisation and comparative studies. The total antioxidant activity of the extract was determined as 8.84±0.10, 4.96 ±0.05, 34.64 ±0.69, and 18.27 ±0.11 mg TE/g dry weight by DPPH, FRAP, CUPRAC, and ABTS assays, respectively. The total phenolic content was 26.25 mg GAE/g dry weight, and LC-MS/MS analysis revealed a high quinic acid content (93.395 mg/g). ICP-MS analysis showed high levels of essential minerals (Na, Mg, K, Ca, Fe, and Al), while toxic elements such as Cd, Te, and Tl were below the limit of detection, and Pb and Ba were present at very low concentrations. Additionally, the extract significantly reduced A549 lung cancer cell viability in a dose-dependent manner. Overall, Tortum Valley–derived M. fruticosa represents a promising natural bioactive source, and this study highlights the impact of local botanical and environmental variations on the biological activity of the same plant species.
- MakaleAçık Erişim2026Scopus SJRQ2Bioactive profile and mineral composition of fruit extracts from four wild hawthorn (Crataegus spp.) species
Çakır, Özlem; Çınar Topçu, Kübra; Sefali, Abdurrahman; Bayyiğit, İshak; İlhan, Gülçe; Singh, Jyoti
This study compared the antioxidant capacity, total phenolic content (TPC), phenolic composition, lipid oxidation level, and mineral profiles of extracts obtained from four wild hawthorn species (Crataegus tanacetifolia, C. orientalis subsp. orientalis, C. orientalis subsp. szovitsii, and C. monogyna) collected from northeastern Türkiye. Significant interspecific variations were observed in DPPH, FRAP, and CUPRAC assays. While C. monogyna had the highest reducing capacity and TPC, C. orientalis subsp. szovitsii had the highest DPPH activity. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry analysis showed that catechin, epicatechin, chlorogenic acid, and quinic acid were the major phenolics, and were particularly abundant in C. monogyna. Despite having a high phenolic content, C. monogyna had the highest malondialdehyde level, indicating that phenolics alone were insufficient to fully inhibit lipid oxidation. Inductively coupled plasma-mass spectrometry mineral analysis detected high levels of K, Mg, and P in all species. Principal component analysis effectively distinguished the species based on their antioxidant, phenolic, and mineral profiles. Overall, hawthorn fruits were confirmed as valuable sources of natural antioxidants and essential minerals.
AtıfScopus1WoS1
İncele - MakaleYalnız künye2026Scopus SJRQ1Profile of honey, bee pollen and bee bread: comparative shotgun sequencing
Morgil, Hande; Ecem Bayram, Nesrin
The plant origin of the bee products often influences their value. Here, we investigated for the first time how the content of bee bread, a functional food with high nutritional value, differs in terms of plant and bacterial taxa in comparison with bee pollen and honey samples, using shotgun sequencing analysis. Sequencing of honey, bee pollen, and bee bread samples yielded approximately 32.74 Gbp of data. Out of a total of 268,964 abundant genes, 70,280 genes (26.13%) were assigned to a taxonomic group according to the supermajority rule. These taxonomic groups were annotated as follows: Kingdom (84.99%), phylum (75.94%), class (73.26%), order (67.21%), and family (65.11%). It was determined that honey, bee pollen, and bee bread samples consisted of large plant families Poaceae, Fabaceae and Asteraceae. This indicates that these taxa are also favored by bees for visiting, and are therefore used as food sources. These differences vary according to the environmental flora of the apiary and may be site specific. Based on these results, DNA-based next-generation sequencing techniques can be successfully used to identify the plant sources of bee products. Thus, the botanical and geographical origin of bee product samples can be more accurately, reliably and in detail identified. Furthermore, this technique can be successfully used to monitor the regional flora and food safety.
- MakaleAçık Erişim2026Scopus SJRQ3Anatomical and Phytochemical Characterization of Ranunculus pinardii and Ranunculus constantinopolitanus: LC-MS/MS-Based Bioactive Compound Profiling
Feizollahi, Sepideh; Gercek, Yusuf Can; Canlı, Deniz; Basturk, Fatma Nur; Yesil, Yeter; Kösesakal, Taylan; Ecem Bayram, Nesrin
The aim of this study was to characterize Ranunculus pinardii and Ranunculus constantinopolitanus species anatomically and phytochemically. In order to contribute to the taxonomic characterization of R. pinardii and R. constantinopolitanus, anatomical structures of the stem, leaf, and petiole of species were examined with light microscopy. Total phenolic-flavonoid content, antioxidant activity, total anthocyanin, total proanthocyanidin, and individual phenolic compounds of extracts prepared with different solvents were determined. The results showed that the total phenolic contents of the species had a similar range (0.09-0.45 mg GAE/g for R. pinardii and 0.01-0.44 mg GAE/g for R. constantinopolitanus), but R. constantinopolitanus exhibited higher antioxidant activity (29.28 mg TE/g). Higher individual phenolic compound recovery was achieved from R. pinardii compared to R. constantinopolitanus with all solvents, except methanol. Both species were rich in luteolin, a natural flavonoid (163.98-34417.11 µg/kg for R. pinardii and 44.93-129009.44 µg/kg for R. constantinopolitanus). These results indicate that these plants contain bioactive ingredients that may have applications in different fields, such as medicine, cosmetics, and food. In particular, the detection of luteolin compound at high levels in these species compared to other components indicates that optimization studies aimed at the recovery of luteolin are important for future research.
- MakaleYalnız künye2026Scopus SJRQ2Microwave-assisted extraction of pectin from persimmon: physicochemical properties, structural characterization and greenness assessment
Yılmaz, Özlem; Kutlu, Naciye; Kamiloğlu, Aybike; Elbir Abca, Tuğba
In this study, the microwave-assisted extraction (MAE) of pectin from persimmon was optimized using a Box-Behnken experimental design, with pH, extraction time, and liquid-to-solid ratio as independent variables. The optimization results obtained through response surface methodology indicated optimum conditions of pH 1.78, extraction time of 8 min, and a liquid-to-solid ratio of 125 mL/g, corresponding to a predicted pectin yield of 4.28%. In the validation experiment, the actual pectin yield was determined to be 3.79 ± 0.04%, confirming a good agreement with the model predictions. The degree of esterification, methoxyl content, galacturonic acid and ash contents, water- and oil-holding capacities, antioxidant activity, rheological behavior, and structural properties (FT-IR, DSC, and 1H NMR) of the samples obtained under optimal MAE and conventional extraction conditions were compared. Based on the degree of esterification, both samples were classified as high-methoxyl, with galacturonic acid contents of 41% and 42% for the MAE and conventionally extracted samples, respectively; both values were below the ≥ 65% level specified for commercial food-grade pectin. The ash content was higher in the MAE sample (7.22%) than in the conventionally extracted sample (1.56%). MAE resulted in a pectin-rich complex with a relatively low galacturonic acid content and higher antioxidant activity than the conventionally extracted sample. Furthermore, the MAE sample exhibited higher water-holding capacity but lower oil-binding capacity than the conventionally extracted sample. Rheological analysis showed that the MAE sample exhibited higher apparent viscosity and shear stress than the conventionally extracted sample. FT-IR analysis revealed more pronounced absorption bands in the MAE sample, suggesting structural differences. DSC analysis revealed differences in the thermal behavior of the MAE and conventionally extracted samples. Additionally, 1H NMR analysis supported the presence of characteristic pectic structural features in both samples. The greenness assessment of the method was assessed using the Greenness Evaluation Metric for Analytical Methods (GEMAM), which yielded an overall score of 7.482, indicating a high level of environmental acceptability. Overall, MAE can be considered an efficient and sustainable approach for obtaining a pectin-rich complex with antioxidant activity from persimmon.
- MakaleYalnız künye2026Scopus SJRQ1Optimization of Antioxidant Recovery from Deep Eutectic Solvent Phase via Back-Extraction Using Taguchi Method
Kamiloğlu, Aybike; Elbir Abca, Tuğba; Kutlu, Naciye
This study investigates the use of deep eutectic solvents (DESs) via ethyl acetate-mediated liquid–liquid extraction and the optimization of the process using the Taguchi method for the recovery of antioxidant compounds from tea industry waste. In the study, choline chloride was used as a hydrogen bond acceptor; lactic acid, glycerol, and ethanol were used as hydrogen bond donors; and different ratios of water (10–30%) were added to reduce the viscosity of the DESs. Ultrasound-assisted extraction results showed that lactic acid-based DESs provided the highest total phenolic content and antioxidant activity due to their ability to break down plant cell walls. However, in the recovery phase carried out with ethyl acetate, it was determined that ethanol-based DESs (ChCl:EtOH) were more effective in transferring antioxidants to the organic phase due to their looser molecular structure. Optimization using the Taguchi L9 orthogonal array revealed the following results: ChCl:EtOH solvent, 2:1 ethyl acetate/extract ratio, 20% water ratio, and 30-min shaking period were determined as the optimal conditions. Under these conditions, the model predicted a yield of 93.34%, and experimental validation revealed a recovery yield of 85.70 ± 1.13%. The results demonstrate that this developed green extraction method offers a sustainable alternative for converting tea waste into high value-added functional components.
- MakaleAçık Erişim2026Scopus SJRQ1Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Polyphenols From Tea Parts: A Green Assessment
Gerçek, Yusuf Can; Baştürk, Fatma Nur; Gıdık, Betül; Abca, Tuğba Elbir; Yurtvermez, Bayram; Fuente-Ballesteros, Adrián; Kutlu, Naciye; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have emerged as green alternatives to conventional solvents for extracting bioactive compounds from plants. This study evaluated nine choline chloride–based DESs for recovering antioxidant polyphenols from four parts of the tea plant (Camellia sinensis): seed, bud, leaf, and flower. Total phenolic content, antioxidant activity, and individual polyphenolic profiles were determined, and the greenness of the sample preparation was assessed using AGREEprep. The highest total phenolic content (53.28 mg GAE/g) and antioxidant activity (141.17 mg TE/g) were obtained from leaves using ChCl:formic acid, outperforming ethanol and methanol. Leaves also exhibited the highest total quantified polyphenolic content (up to 95,813 mg/kg DW, corresponding to the sum of the quantified polyphenols), with epicatechin gallate and gallocatechin + epigallocatechin being the major contributors. Flowers and buds showed notable polyphenolic diversity, while seeds contained the lowest levels. Statistical analysis confirmed clear clustering patterns according to plant part and solvent type. AGREEprep evaluation yielded scores up to 0.54, highlighting the environmental benefits of DES-based extraction. We demonstrated that tea leaves were the richest source of bioactive polyphenols, and selected DESs, particularly formic acid–based and ethylene glycol–based, exhibited superior efficiency and sustainability compared to conventional solvents. These findings support the wider adoption of DESs for green, high-yield extraction of pharmacologically important compounds from tea and other medicinal plants.
- MakaleYalnız künye2026Scopus SJRQ2Optimizing microwave drying parameters for rhododendron bee pollen: effects on antioxidant potential, polyphenols, and proteins
Kutlu, Naciye; Gercek, Yusuf Can; Ecem Bayram, Nesrin
Monofloral Rhododendron spp. bee pollen is a remarkable bee product due to its functional components as well as its potential pharmacological effects. In this study, Rhododendron spp. bee pollen was dried by the microwave method as an alternative to the conventional drying method. Different combinations of microwave powers (140, 210 and 280 W) and sample weights (10, 20 and 30 g) applied during microwave drying (MWD) were determined by Central Composite Design. Drying time, total protein, total carotenoid, total phenolic-flavonoid compounds, and proanthocyanidin yields of dried pollen were determined and antioxidant activity tests (CUPRAC and DPPH•) were performed. The analysis results were optimized by response surface methodology (RSM) and the optimum drying conditions were obtained when the microwave power was 280 W and the sample weight was 30 g. The optimal conditions have been established to reduce drying time and enhance the content of bioactive compounds. As a result of optimization, the composite desirability (d) was found to be 0.8. Experimental and predicted values were close to each other. In conclusion, MWD has demonstrated efficacy as a drying technique by significantly reducing drying time while maintaining and enhancing the yields of bioactive compounds in monofloral Rhododendron spp. bee pollen. In comparison to conventional techniques, the application of MWD in the drying process of monofloral Rhododendron spp. bee pollen is demonstrably more efficient and sustainable.
Tam Kayıt
| Alan | Değer | Dil |
|---|---|---|
| dc.date.accessioned | 2026-10-29T17:14:21.542808733Z | |
| dc.date.available | 2026-10-29T17:14:21.542808733Z | |
| dc.identifier.uri | https://rdlopen.rawdatalibrary.net/r/bayburt/10212 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.title | Gıda İşleme Bölümü | tr |
| dc.title | Department of Food Processing | en |
| organization.identifier.code | bayburt-aydintepe-gida-isleme | |
| organization.unitType | department | |
| rdl.unit.alias | \bgida isleme bolumu\b | |
| rdl.unit.alias | \bfood processing\b |