Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Ay Şal, Fulya(1)
- Bayraktar, Bulent(1)
- Beldüz, Ali Osman(1)
- Can, Zehra(1)
- Dirican, E.(1)
- Dirican, Ebubekir(1)
- Güler, Halil İbrahim(1)
- Halıcı, Z.(1)
- Halıcı, Zekai(1)
- Kara, Yakup(1)
- Karabulut Uzuncakmak, S.(1)
- Karabulut Uzunçakmak, Sevgi(1)
- Kerget, Ferhan(1)
- Kesmez Can, F.(1)
- Kolayli, Sevgi(1)
- Korkmaz, Serol(1)
- Naldan, M.E.(1)
- Naldan, Muhammet Emin(1)
- Parmaksiz, Ayse(1)
- Sait, Ahmet(1)
- Uzunçakmak, Sevgi Karabulut(1)
- Yildiz, Oktay(1)
- Çanakçi, Sabriye(1)
Yayınlar
- MakaleYayıncıda açık erişim2022Scopus SJRQ4Investigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patients
Karabulut Uzuncakmak, S.; Dirican, E.; Naldan, M.E.; Kesmez Can, F.; Halıcı, Z.
Background: COVID-19 pandemic spread around the world like an infectious disease that presents waved effects on patients. Some patients needed ICU and respiratory support. Some patients only had flu-like symptoms. Cytokine storm and elevated ROS were serious problems for treatment. Apoptotic genes and CYP Family are part of these mechanisms. Aim: In this study, our aim was to examine the gene expression CYP2E1 and Caspase-3 in patients with COVID-19 infection. Method: 60 COVID-19(+) patients (ICU and non-ICU patients) and 30 healthy volunteers were enrolled to study. To measure the level of gene expression qPCR was used. The 2-ΔΔCt method was utilized to analyze gene expression. Results: The expression of CYP2E1 and Caspase-3 genes showed a significant discrepancy between patients and healthy individuals. Caspase-3 expression increased (p=0,0041) but CYP2E1 expression decreased (p=0,0214) in COVID-19 patients compared to healthy individuals. Both levels of gene expression were lower in patients with affected lungs than patients with unaffected lungs (p<0,05). Laboratory findings including d-Dimer, LDH, platelet count, lymphocyte count were related to both gene expressions (p<0,05). We found no correlation between CYP2E1 and Caspase-3 expressions. Conclusion: The expression of Caspase-3 demonstrated apoptotic situations of patients but was not related to the CYP2E1 expression level. CYP2E1 gene expression is an important actor to metabolize endogens and xenobiotics however, COVID-19 patients demonstrated decreased CYP2E1 expression. CYP2E1 and Caspase-3 gene expression levels may be used as a diagnostic tool for COVID-19 patients.
AtıfScopus8WoS5OpenAlex7
İncele - MakaleAçık Erişim2022Scopus SJRQ2Preliminary investigation of gene expression levels of PAPP-A, STC-2, and HIF-1α in SARS-Cov-2 infected patients
Karabulut Uzunçakmak, Sevgi; Naldan, Muhammet Emin; Dirican, Ebubekir; Kerget, Ferhan; Halıcı, Zekai
Background: Coronavirus-19 is still considered a pandemic that influences the world. Other molecular alterations should be clearer besides the increasing cytokine storm and pro-inflammatory molecules. Hypoxic conditions that induce HIF-1α lead to stimulate gene expression of STC-2 that targets PAPP-A expression. This study aimed to determine gene expression levels of PAPP-A, STC-2, and HIF-1α in COVID-19 infection. We also aimed to reveal the relationship of these genes with laboratory and clinical data of COVID-19 patients. Materials and Results: We extracted RNA from peripheral blood samples of COVID-19(+) and COVID-19(−) individuals. The real-time PCR method was used to measure mRNA expression of PAPP-A, STC-2, and HIF-1α. Gene expression analysis was evaluated by the 2−ΔΔCt method. PAPP-A, STC-2, and HIF-1α mRNA expressions of severe patients were higher than healthy individuals (p = 0.0451, p = 0.4466, p < 0.0001, respectively). Correlation analysis of gene expression patterns of severe patients demonstrated a positive correlation between PAPP-A and STC-2 (p < 0.0001, r = 0.8638). Conclusion: This is the first study that investigates the relation of PAPP-A, STC-2, and HIF-1α gene expression in patients with COVID-19 infection. Besides the routine laboratory findings, PAPP-A, STC-2, and HIF-1α mRNA expressions may be considered to patients’ prognosis as a sign of increased cytokines and pro-inflammatory molecules.
AtıfScopus6WoS6OpenAlex8İncele - MakaleAçık Erişim2022Scopus SJRQ3Investigation of the recovery efficiency of CeUF method through RT-qPCR quantification of inactivated SARS-CoV-2 in untreated wastewater
Sait, Ahmet; Korkmaz, Serol; Parmaksiz, Ayse; Bayraktar, Bulent
Wastewater-based disease monitoring is an early warning system and a surveillance tool for infectious disease outbreaks regarding pathogens with pandemic potential. This study aimed at investigating the recovery efficiency of centrifugal ultrafiltration (CeUF), which is one of the most-used virus concentration methods, for inactive severe acute respiratory syndrome coronavi-rus 2 (SARS-CoV-2) added to wastewater. Inactivated SARS-CoV-2 was inoculated into untreated wastewater at different concentrations (4 × 103, 8 × 103, 16 × 103, 24 × 103 and 32 × 103 gene copy/ µL) and concentrated through ultrafiltration with a disposable centrifugal filter device. Total nucleic acids in concentrated filtrates were extracted and isolated by an automated system. In isolates, total RNA was measured by a UV/VIS spectrophotometer, and the recovered virus was quanti-fied by RT-qPCR with two gene regions (N1 and N2). The recovery rates were between 11% and 17.8% (mean 15.1%, CV below 15%). While there were positive correlations among the inoculated virus, total RNA and recovered virus, there was no correlation and linearity between the recovery rates. Despite limited recovery rates, CeUF integrated with RT-qPCR quantification can be a valid assay for monitoring SARS-CoV-2 in wastewater, and an early warning system.
AtıfScopus2WoS2OpenAlex3İncele - Derleme MakaleAçık Erişim2022Scopus SJRQ3SARS-CoV-2 Infection and Candidate Biomarkers
Uzunçakmak, Sevgi Karabulut
Severe acute respiratory syndrome coronavirus 2 is a virus that can still infect individuals and whose deadly effects continue despite the current vaccines and drugs. Since 2019, many studies on the pathogenesis of the disease have been completed and continue to be done. In addition to the diagnosis and treatment of the disease, many molecules that can be markers of the disease have been investigated. In the early stages of the pandemic, many nonspecific and infection-related laboratory findings and chest computed tomography were used to obtain information about the diagnosis of the disease. The more individual molecules became associated with the disease yet. The purpose of this review is to summarize the impact and role of many molecules associated with coronavirus disease-2019 infection that have been previously used and newly revealed. Numerous studies are summarized in this review. The obtained data show that previously used laboratory findings and new potential biomarkers are not specific to the disease. New potential biomark-ers have been associated with the severity of the disease itself, as can be seen with lung imaging and even with routine laboratory findings. One of the important points that are seen frequently in studies is that the effectiveness of these molecules has been shown not only in coronavirus disease-2019 infection but also in many other diseases. This removes the pathogenesis of the disease from being a unique mechanism created by the Severe acute respiratory syndrome coronavirus 2 and provides a general perspective formed by viral or bacterial infections. However, there are still many molecular changes that need to be investigated. Future studies will continue to update themselves with the mutations of the virus.
AtıfScopus1WoS1
İncele - MakaleAçık Erişim2021Scopus SJRQ2Targeting cov-2 spike rbd and ace-2 interaction with flavonoids of anatolian propolis by in silico and in vitro studies in terms of possible covid-19 therapeutics
Güler, Halil İbrahim; Ay Şal, Fulya; Can, Zehra; Kara, Yakup; Yildiz, Oktay; Beldüz, Ali Osman; Çanakçi, Sabriye; Kolayli, Sevgi
Propolis is a multi-functional bee product rich in polyphenols. In this study, the inhibitory effect of Anatolian propolis against SARS-coronavirus-2 (SARS-CoV-2) was investigated in vitro and in silico. Raw and commercial propolis samples were used, and both samples were found to be rich in caffeic acid, p-coumaric acid, ferulic acid, t-cinnamic acid, hesperetin, chrysin, pinocembrin, and caffeic acid phenethyl ester (CAPE) at HPLC-UV analysis. Ethanolic propolis extracts (EPE) were used in the ELISA screening test against the spike S1 protein (SARS-CoV-2): ACE-2 interaction for in vitro study. The binding energy values of these polyphenols to the SARS-CoV-2 spike and ACE-2 protein were calculated separately with a molecular docking study using the AutoDock 4.2.6 program. In addition, the pharmacokinetics and drug-likeness properties of these eight polyphenols were calculated according to the SwissADME tool. The binding energy value of pinocembrin was highest in both receptors, followed by chrysin, CAPE, and hesperetin. Based on the in silico modeling and ADME (absorption, distribution, metabolism, and excretion) behaviors of the eight polyphenols, the compounds exhibited the potential ability to act effectively as novel drugs. The findings of both studies showed that propolis has a high inhibitory potential against the Covid-19 virus. However, further studies are now needed.
AtıfScopus35WoS29OpenAlex39İncele