Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleYalnız künye2023Scopus SJRQ3A comparison of the associations between bone turnover markers and different sports fields: combat versus team sports
Yıldız, K.; Uzunçakmak, S. Karabulut; Halıcı, Z.; Özçelik, C.; Yazıcı, A.G.
Sedentary life brings risks that include osteoporosis, while physical activity has many benefits for health in general and the skeletal system. Irisin is a hormone-like myokine regulated by mechanical force and it contributes to cortical and trabecular bone mineral density. This study aimed to investigate the effects of different types of sports on bone health by measuring elite athletes’ fibronectin type III domain 5 (FNDC5), N-terminal collagen type I extension propeptide (PINP), and C-terminal cross-linking telopeptide of type I collagen (CTXI) levels. Combat sports athletes, team sports athletes, and sedentary control subjects were enrolled. Serum levels of proteins were measured by ELISA. FNDC5 levels of elite athletes were higher compared to the sedentary controls (P=0.0014). The highest FNDC5 level was measured in wrestlers among all considered groups. The PINP levels of the athletes were also higher than those of the sedentary control group (P=0.1431). The highest PINP level was determined in boxers. The CTXI levels of the athletes were lower than those of the sedentary control (P<0.0001). The lowest CTXI level was seen in boxers. The FNDC5 and PINP levels of combat sports athletes were higher than those of team sports athletes (P=0.0134 and P=0.0262, respectively), while the CTXI levels of combat sports athletes were lower than those of team sports athletes (P<0.0001). Our results indicated that FNDC5, PINP, and CTXI levels are associated with physical activity. The effect of the exercises performed by athletes of specific sports on bone health has not been studied in much detail before. Combining different exercises for athletes of a particular sport may be more beneficial for bone health.
- MakaleYayıncıda açık erişim2022Scopus SJRQ4Investigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patients
Karabulut Uzuncakmak, S.; Dirican, E.; Naldan, M.E.; Kesmez Can, F.; Halıcı, Z.
Background: COVID-19 pandemic spread around the world like an infectious disease that presents waved effects on patients. Some patients needed ICU and respiratory support. Some patients only had flu-like symptoms. Cytokine storm and elevated ROS were serious problems for treatment. Apoptotic genes and CYP Family are part of these mechanisms. Aim: In this study, our aim was to examine the gene expression CYP2E1 and Caspase-3 in patients with COVID-19 infection. Method: 60 COVID-19(+) patients (ICU and non-ICU patients) and 30 healthy volunteers were enrolled to study. To measure the level of gene expression qPCR was used. The 2-ΔΔCt method was utilized to analyze gene expression. Results: The expression of CYP2E1 and Caspase-3 genes showed a significant discrepancy between patients and healthy individuals. Caspase-3 expression increased (p=0,0041) but CYP2E1 expression decreased (p=0,0214) in COVID-19 patients compared to healthy individuals. Both levels of gene expression were lower in patients with affected lungs than patients with unaffected lungs (p<0,05). Laboratory findings including d-Dimer, LDH, platelet count, lymphocyte count were related to both gene expressions (p<0,05). We found no correlation between CYP2E1 and Caspase-3 expressions. Conclusion: The expression of Caspase-3 demonstrated apoptotic situations of patients but was not related to the CYP2E1 expression level. CYP2E1 gene expression is an important actor to metabolize endogens and xenobiotics however, COVID-19 patients demonstrated decreased CYP2E1 expression. CYP2E1 and Caspase-3 gene expression levels may be used as a diagnostic tool for COVID-19 patients.
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İncele - Derleme MakaleAçık Erişim2020Scopus SJRQ1Effects of umbelliferone isolated from the Ferulago pauciradiata Boiss. & Heldr. Plant on cecal ligation and puncture-induced sepsis model in rats
Kutlu, Z.; Celik, M.; Bilen, A.; Halıcı, Z.; Yıldırım, S.; Karabulut, S.; Karakaya, S.; Bostanlık, Delimustafaoğlu F.; Aydın, P.
Sepsis is a pathophysiological event involving systemic inflammatory response syndrome, multiple organ failure syndromes, and tissue damage. Overproduction of free radicals as a result of tissue damage during sepsis contributes to cellular toxicity, organ failure, and even mortality. Antioxidants, which scavenge free radicals, play a protective role against various diseases. Previous studies have shown that umbelliferone (UF) has antioxidant and anti-inflammatory effects. Since oxidative stress is naturally associated with sepsis-induced organ dysfunction, the application of antioxidant compounds could potentially illuminate the pathophysiology of sepsis, which does not yet have an effective treatment. The sepsis model induced by cecal ligation and puncture (CLP) was applied to rats. Different doses of UF (10░mg/kg, 20░mg/kg, and 40░mg/kg) on oxidant-antioxidant in septic rats, mRNA of inflammatory mediators such as tumor necrosis factor- α (TNF-α) and interleukin (IL)-1 its effects on expression levels were evaluated in lung, kidney, and liver tissues. When the lung, kidney, and liver tissues of septic rats were compared with those of the control group, it was found that UF administration increased dose-dependent superoxide dismutase activity and glutathione levels and significantly decreased malondialdehyde levels. The effects of UF administration on oxidative parameters were dose-dependent. The 40░mg/kg UF dose showed greater anti-oxidative properties than the 20░mg/kg and 10░mg/kg doses for all the evaluated parameters. Further, the TNF- α mRNA expression of the CLP +40░mg/kg group was reduced to a level comparable to that of the control group. UF has been found to be an effective molecule in reducing oxidative stress by supporting endogenous antioxidants and enhancing the scavenging effects of free radicals. The potent antioxidant property of UF may also be related to the suppression of the cytokine cascade during sepsis. The results suggest that UF administration may represent a new treatment for the prevention of lung, kidney and liver damage caused by septic conditions.
AtıfScopus22WoS21OpenAlex23İncele