Sürdürülebilir Kalkınma Amaçları
Yazarlar
- Kutlu, Naciye(5)
- Ecem Bayram, Nesrin(3)
- Gerçek, Yusuf Can(3)
- Abca, Tuğba Elbir(2)
- Bayram, Sinan(2)
- Kamiloğlu, Aybike(2)
- Yilmaz, Özlem(2)
- Çelik, Saffet(2)
- Baştürk, Fatma Nur(1)
- Elbir Abca, Tuğba(1)
- Erdem, G.M.(1)
- Fuente-Ballesteros, Adrián(1)
- Gıdık, Betül(1)
- Isci, A.(1)
- Kutlu, N.(1)
- Kırkıncı, Seran(1)
- Sakiyan, O.(1)
- Yilmaz, M.S.(1)
- Yurtvermez, Bayram(1)
Yayınlar
- MakaleAçık Erişim2026Scopus SJRQ1Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Polyphenols From Tea Parts: A Green Assessment
Gerçek, Yusuf Can; Baştürk, Fatma Nur; Gıdık, Betül; Abca, Tuğba Elbir; Yurtvermez, Bayram; Fuente-Ballesteros, Adrián; Kutlu, Naciye; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) have emerged as green alternatives to conventional solvents for extracting bioactive compounds from plants. This study evaluated nine choline chloride–based DESs for recovering antioxidant polyphenols from four parts of the tea plant (Camellia sinensis): seed, bud, leaf, and flower. Total phenolic content, antioxidant activity, and individual polyphenolic profiles were determined, and the greenness of the sample preparation was assessed using AGREEprep. The highest total phenolic content (53.28 mg GAE/g) and antioxidant activity (141.17 mg TE/g) were obtained from leaves using ChCl:formic acid, outperforming ethanol and methanol. Leaves also exhibited the highest total quantified polyphenolic content (up to 95,813 mg/kg DW, corresponding to the sum of the quantified polyphenols), with epicatechin gallate and gallocatechin + epigallocatechin being the major contributors. Flowers and buds showed notable polyphenolic diversity, while seeds contained the lowest levels. Statistical analysis confirmed clear clustering patterns according to plant part and solvent type. AGREEprep evaluation yielded scores up to 0.54, highlighting the environmental benefits of DES-based extraction. We demonstrated that tea leaves were the richest source of bioactive polyphenols, and selected DESs, particularly formic acid–based and ethylene glycol–based, exhibited superior efficiency and sustainability compared to conventional solvents. These findings support the wider adoption of DESs for green, high-yield extraction of pharmacologically important compounds from tea and other medicinal plants.
- MakaleAçık Erişim2024Scopus SJRQ1Ultrasonic assisted extraction of water-soluble vitamins from minor components of bee pollen with deep eutectic solvents (DESs) as green solvent
Çelik, Saffet; Gerçek, Yusuf Can; Kutlu, Naciye; Bayram, Sinan; Kırkıncı, Seran; Ecem Bayram, Nesrin
In this study, different deep eutectic solvents (DESs) were used for vitamin extraction from bee pollen. The DES combination with the highest total amount of individual vitamins was choline chloride: ethylene glycol (molar ratio 1:2). The response surface method was then used to optimize the extraction conditions. The independent variables used for optimization were ultrasonic power, time, and temperature, while the dependent variables were the total individual amino acids, total individual phenolic compounds, and total individual vitamin contents. Spectrophotometric analyses and antimicrobial activity were also determined in the extracts. In addition, AGREEprep, ComplexGAPI and BAGI tools were used to evaluate the greenness of the method. The results obtained with DES were compared with water, ethanol, and methanol extracts, which were considered control groups. As a result of optimization, the optimum conditions were found to be 75 % for ultrasonic power, 71.5 min for sonication time, and 40 °C for ultrasonic bath temperature. The model's composite desirability value (d) was found to be 0.81. The predicted value and the experimental values were close to each other. Furthermore, the most dominant vitamin in DES extracts was ascorbic acid, the most dominant amino acid was proline, and the most dominant phenolic compound was myricetin. The amounts of these compounds were higher than those obtained from water, ethanol, and methanol extracts. The spectrophotometric analysis results obtained higher yields with DES compared to the control groups. Antimicrobial activity analysis showed that pollen extracts prepared using choline chloride: ethylene glycol had a weak inhibitory effect for Gram-positive and Gram-negative bacteria but not yeast-like fungal strains. In conclusion, DES has been successfully used to extract vitamins and other bioactive compounds from bee pollen. The results obtained showed that DESs can be an alternative to organic solvents for the extraction of vitamins, which are among the minor components of bee pollen. Besides, the potential of the application different DESs for more efficient extraction of vitamins and components with important pharmaceutical properties from bee pollen needs to be evaluated in detail.
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İncele - MakaleAçık Erişim2024Scopus SJRQ1Ultrasound-assisted deep eutectic solvent extraction of bioactive compounds from persimmon calyx
Kutlu, Naciye; Kamiloğlu, Aybike; Abca, Tuğba Elbir; Yilmaz, Özlem
Abstract: This study aimed to investigate the ultrasound-assisted extraction of bioactive compounds from persimmon (Diospyros kaki) calyx by deep eutectic solvents (DES) with different molar ratios. For this reason, the prepared DES extracts’ total phenolic–flavonoid compounds and antioxidant activities (1,1-diphenyl-2-picrilhydrazyl radical scavenging activity [DPPH•], Cupric Reducing Antioxidant Capacity (CUPRAC), and ferric reducing antioxidant power [FRAP]) were investigated as a result of the experimental design and optimization study conducted for this purpose. A sonication time of 20 min was determined as the optimal condition. Under these conditions, a molar ratio of 1.9:1 (lactic acid:choline chloride) and a water ratio of 70% provided the highest phenolic/flavonoid compounds and antioxidative activity. Correlations among water ratio, molar ratio, and sonication time were determined using principal component analysis (PCA). In conditions where total flavonoid compound, FRAP, and DPPH• are high due to PCA, it can be concluded that the sonication time is at high level; on the contrary, the water and molar ratios are at low level. In conclusion, ultrasound-assisted extraction using DES proved effective in persimmon calyx. Therefore, it can be recommended to use these environmentally friendly green solvents as an alternative to organic solvents in preparing extracts in various fields. Practical Application: This study shows the effectiveness of the ultrasound-assisted green extraction method using persimmon calyx specified as waste. These findings are compelling in the food industry in terms of consumers being now aware of green technology and the discovery that calyx is a good source of bioactive compounds.
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İncele - MakaleYalnız künye2024Scopus SJRQ1Ultrasound assisted choline chloride-based deep eutectic solvent extraction of bioactive compounds from salsify (Tragopogon buphthalmoides (DC.) boiss. Var. Latifolius Boiss.): A Box–Behnken design approach for optimization
Yilmaz, Özlem; Elbir Abca, Tuğba; Kamiloğlu, Aybike; Kutlu, Naciye
This study aimed to investigate the ultrasound-assisted extraction of bioactive compounds from salsify using deep eutectic solvents (DESs) with different molar ratios. For this reason, total phenolic (TPC), total flavonoid (TFC) compounds, and antioxidant activities (1,1-diphenyl-2-picrilhydrazyl radical scavenging activity (DPPH•), and ferric reducing antioxidant power (FRAP)) of extracts prepared with DES were investigated with an experimental design and optimization study conducted for this purpose. An extraction time of 40.15 min was determined as the optimal condition. Under these conditions, a molar ratio of 3:1 (acetic acid:choline chloride) and a water ratio of 42.17 % provided the highest phenolic/flavonoid compounds and antioxidant activity. Correlations between water ratio, molar ratio, and sonication extraction time were determined using principal component analysis (PCA). In conditions where TPC, FRAP, and DPPH• were high according to PCA, it can be concluded that the extraction time, water ratio, and molar ratio are at high levels; contrarily, TFC is at a low level. In conclusion, ultrasound-assisted extraction using DES proved effective for salsify. Therefore, choline chloride-based DESs, can be recommended as environmentally friendly green solvents and an alternative to organic solvents for the extraction of bioactive compounds from salsify.
AtıfScopus11WoS8OpenAlex10İncele - MakaleAçık Erişim2024Scopus SJRQ2An optimization study for amino acid extraction from bee bread using choline chloride-acetic acid deep eutectic solvent and determination of individual phenolic profile
Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Bayram, Sinan; Ecem Bayram, Nesrin
In this study, the aim was to optimize the extraction conditions (molar ratio, time, temperature) in order to maximize the total individual amino acid (TAA) yield from bee bread, which is a fermented bee product, using choline chloride-acetic acid deep eutectic solvent (DES). In addition to the phenolic profile and amino acid profile investigated by LC-MS/MS, some spectrophotometric (total phenolic content, total flavonoid content, total proanthocyanidin content) and bioactivity (antioxidant/antimicrobial activity) tests were carried out on the extracts. Optimum conditions were determined as 1:2 for molar ratio, 15 min for time and 10 °C for temperature with optimization based on the TAA values of the different extracts (d: 0.99). Molar ratio and temperature were found to have statistically significant effects on TAA values. At optimum conditions, the amount of TAA obtained with DES (29.678 g/kg) was found to be approximately 35.2% and 54.4% higher than that obtained with ethanol and methanol extracts, respectively. All these results indicate that choline chloride-acetic acid DES can be chosen when preparing bee bread extracts, which are evaluated for different areas of use such as food and health due to active ingredient content. Higher active ingredients can be extracted by trying different DES combinations.
AtıfScopus7WoS9OpenAlex11İncele - MakaleYayıncıda açık erişim2022Scopus SJRQ3EFFECTS OF MOLAR RATIO, FREQUENCY, WATER CONTENT AND TEMPERATURE ON DIELECTRIC PROPERTIES OF DEEP EUTECTIC SOLVENTS
Kutlu, N.; Yilmaz, M.S.; Erdem, G.M.; Sakiyan, O.; Isci, A.
The aim of the study is to determine the effect of process parameters on dielectric propertiesof deep eutectic solvents (DESs), which have beenwidely used in recent years. In this study, DESs wereprepared using choline-chloride as the hydrogenbondacceptor and glycerol, formic acid and aceticacid as the hydrogen-bond donor. The effect of differentprocess parameters such as molar ratio (1:2, 1:3and 1:4), water content (15%, 30% and 45%), temperature(25, 50 and 75 °C) and frequency on dielectricproperties of the DESs were examined. The highestdielectric constant values were found to be38.13±0.54, 44.96±0.07 and 46.74±0.02 for glycerol,formic acid and acetic acid, respectively for the processconditions of 25 °C temperature, 45% water contentand 1:4 molar ratio (at 2450 MHz). Moreover, forall DESs, it was found that a decrease in water contentresulted in a decrease in both dielectric constant andloss factor values. This can be explained by the absenceof free water molecules which are responsiblefrom dipole rotation mechanism. In light of the results,if DES will be used in microwave processes, formicor acetic acid containing DESs might give moresuccessful results compared to the ones with glycerol
AtıfScopus7WoS6OpenAlex8İncele