Sürdürülebilir Kalkınma Amaçları
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- MakaleYalnız künye2022Scopus SJRQ2Organic acids and their derivatives: minor components of bee pollen, bee bread, royal jelly and bee venom
Çelik, Saffet; Gerçek, Yusuf Can; Özkök, Aslı; Ecem Bayram, Nesrin
This study aimed to determine the organic acid profiles of bee products such as royal jelly, bee venom, bee pollen and bee bread, as well as to verify the method employed in the study. For this purpose, royal jelly, bee venom, bee pollen and bee bread samples were obtained from different locations, and 55 individual organic acids were determined using the liquid chromatography technique coupled with tandem mass spectrometry (LC–MS/MS), and method verification was carried out. Moreover, principal component and hierarchical cluster analyses were performed to compare the organic acid content of bee products and evaluate the overall variation. According to the results, the order of the total organic acid profiles was determined as bee venom (4141 mg/kg-6260 mg/kg) > bee bread (736–990 mg/kg) > bee pollen (837–1503 mg/kg) > royal jelly (192–1947 mg/kg). Although citric acid (423–41,519 mg/kg) was dominantly detected in samples among the organic acids screened. It is thought that the results obtained will contribute to scientific studies carried out to determine the authenticity of bee products and their standardization.
AtıfScopus24WoS21OpenAlex26
İncele - MakaleYalnız künye2022Scopus SJRQ1Bioactive compounds of deep eutectic solvents extracts of Hypericum perforatum L.: Polyphenolic- organic acid profile by LC-MS/MS and pharmaceutical activity
Bayram, Sinan; Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Ecem Bayram, Nesrin
Deep eutectic solvents (DESs) are known as environmentally-friendly solvents that have emerged as an alternative to conventional organic solvents in recent years. In this study, the effects of ten different DESs on the extraction of bioactive components from the Hypericum perforatum were investigated. Total phenolic content, total flavonoid content, phenolic profile (32 phenolics), organic acid profile (52 organic acids), as well as antioxidant and antimicrobial activities of H. perforatum extracts prepared using DESs and ultrasonic-assisted extraction method were determined. Total phenolic, total flavonoid content, and antioxidant activities of extracts were determined between 3.10 ± 0.86–16.64 ± 2.09 mg GA/g, 0.02 ± 0.00–12.29 ± 0.30 mg QE/g, and 8.40 ± 0.50–44.78 ± 2.66 mg TE/g, respectively. DES2 (choline chloride:ethylene glycol) and DES7 (choline chloride:lactic acid) demonstrated higher extraction success than other DESs for extracting phenolics and organic acids, respectively. DESs prepared using organic acid as hydrogen bond donor were found to have an antibacterial effect against all bacterial samples. However, the inhibitory effect against yeast-like fungi was observed in a narrower spectrum. Moreover, the results from principal component analysis also confirmed that the type of solvent used for extraction has a significant influence on the phenolics, organic acid, and other parameters. As a result, it was seen that DES components directly affect the extraction efficiency of target compounds from H. perforatum. This is a first and original report about use of DESs in extracting different bioactive components from H. perforatum.
AtıfScopus33WoS31OpenAlex32İncele - MakaleAçık Erişim2021Scopus SJRQ3KESTANE (Castanea sativa) BALI ÖRNEKLERİNİN BOTANİK ORİJİNLERİNİN DOĞRULANMASI VE TOPLAM POLEN SAYILARI
Özkök, Aslı; Ecem Bayram, Nesrin
Bu çalışmada, Türkiye’nin Zonguldak ilinin farklı lokasyonlarından toplanan bal örneklerinin (n=9) botanik orijinlerinin palinolojik analizle tespit edilmesi ve toplam polen sayılarının saptanması amaçlanmıştır. Ek olarak toplanan bal örneklerinin Türk Gıda Kodeksi Bal Tebliği (No:2020/7)’nde kestane balı numuneleri için verilen polen içeriği kriteri ile uyumluluğu tartışılmıştır. Melissopalinolojik analizler neticesinde bal örnekleri kestane balı (n=7), karışık çiçek balı (n=2) ve geven balı (n=1) olarak etiketlenmiştir. Kestane balı olarak tespit edilen örneklerin 10 gr baldaki toplam polen sayısı (TPS-10) 41722-647312 arasında saptanmış olup bu bal örneklerinin çok iyi, iyi ve normal kalitede bal örnekleri olduğu belirlenmiştir. Ayrıca elde edilen sonuçlar kestane balı olarak toplanan bal örneklerinin %33’ünün ulusal gıda kodeksinde kestane botanik orijinine sahip unifloral bal tipleri için rapor edilen minimum polen içeriğine sahip olmadığını göstermiştir. Bu sonuçlar özellikle ülkemizde diğer polifloral çiçek ballarına kıyasla daha yüksek fiyata satılan kestane gibi unifloral bal tipleri için botanik orijin tespit analizinin zorunlu hale getirilmesinin haksız rekabetin önlenmesi açısından ne denli önemli olduğunu göstermiştir. Bu nedenle, ülkemizde üretilen fakat minimum polen içeriği Türk Gıda Kodeksi Bal Tebliği (No:2020/7)’nde belirtilmeyen lavanta ve çörekotu gibi farklı tipte unifloral çiçek balları için de melissopalinolojik çalışmaların yürütülerek polen içeriği değerlerinin belirlenmesi önemli görülmektedir.
AtıfOpenAlex8TR Dizin5
İncele - MakaleAçık Erişim2021Molecular Approach to Detecting Pollen Types In Honey: DNA Barcoding
Ecem Bayram, Nesrin
Nowadays, the demand for bee products has also increased due to the growing interest in natural products. Althoughit is quite easy to adulteration of honey, which is one of these products, it is also very difficult to detect adulteration.In the point of detecting forgery, studies are being carried out specifically to identify the plant sources of honey.Melissopalynological and chemical analyses are methods commonly used in order to identify the botanical origin of honey.Despite the fact that the detection of botanical origin of honey by DNA-based methods which provide faster, simpler andmore reliable results are being carried out in recent years, these researches are very less in Turkey. Unlike morphologicalmethods, which require the visual examination of pollen grains, the recently developed genetic methods have the potentialto increase the resolution and scale of pollen analyses. In this study, the aim was to present cumulative data by compilingthe results of the studies conducted using molecular techniques on honey, and the advantages and disadvantages of thistechnique were evaluated.
AtıfOpenAlex1İncele - MakaleAçık Erişim2021Scopus SJRQ3A study on free-radical scavenging activity, individual phenolic compounds and element concentration of propolis; [Propolisin serbest radikal temizleme aktivitesi, bireysel fenolik bilesik içerigi ve element konsantrasyonu üzerine bir çalisma]
Ecem Bayram, Nesrin
The aim of this study was to assess the quality of five propolis samples obtained from Turkey (TP), China (CP), Brazil (BP1, BP2), and Ethiopia (EP). The phenolic compounds of the propolis were identified and quantified using the liquid chromatography-tandem mass spectrometry technique (LCMS/ MS). In addition, quality parameters such as total flavonoid content, total phenolic content, freeradical scavenging activity and element contents were investigated. As a result of LC-MS/MS analysis, the extracts were sorted as TP> BP1> BP2> CP> EP in terms of the total concentration of individual phenolic compounds. Chlorogenic acid was determined as the dominant compound in BP1 and EP, pcoumaric acid as the dominant compound in the BP2 and trans-ferulic acid as the dominant compound in the samples from CP and TP. The concentration of DPPH was higher in TP whereas the ABTS concentration was almost similar to other propolis extracts. The contents of potassium (K), calcium (Ca), iron (Fe), magnesium (Mg) and sodium (Na) in the propolis samples were in the range of 2416.75- 14416.02 mg/kg, 8.52-613.25 mg/kg, 102.66-1425.82 mg/kg, 523.84-7336.74 mg/kg and 57.65-191.15 mg/kg, respectively. Consequently, it is again supported that chemical characteristics and activity of propolis varies according to its geographical origin with this study.
AtıfScopus1OpenAlex3İncele - MakaleYalnız künye2021Scopus SJRQ2Vitamin, mineral, polyphenol, amino acid profile of bee pollen from Rhododendron ponticum (source of “mad honey”): nutritional and palynological approach
Ecem Bayram, Nesrin
In this study, it was aimed to characterize the pollen collected by bees from the Rhododenron ponticum plant, which is the source of mad honey, in terms of some parameters. In the first stage, the botanical origin of the sample was confirmed by palynological analysis supported by light microscopic images. Then, the chemical profile of Rhododenron ponticum bee pollen was examined in terms of 31 elements, 42 amino acids, 33 phenolics and vitamins A, B1, B2, B5, B6, B7, B12, C, E, K1 and K2. The total individual phenolic compound content of Rhododendron ponticum bee pollen was determined as 11928.23 µg/100 g, total element content as 13997.75 mg/kg, total free amino acid content as 149466.11 µg/g and total vitamin content as 20509.37 µg/100 g. In addition, it was characterized by a low content of vitamin A (retinol), B7, B12, K2, as well as a deficiency of vitamin K1. As a result of analysis with LC-MS-MS, it was found that it contains L-asparagine and myricetin in a significantly higher concentration compared to other amino acids and phenolic compounds. However, the presence of phenolic compounds (myricetin, epicatechin, tyrosol etc.) and amino acids (L-asparagine, gamma-aminobutyric acid, L- Tryptophan etc.) with significant biological activity indicates that this product is a bee product with high medicinal properties. Moreover, the significant presence of Mg, P, Fe and Ca, high K/Na ratio, vitamin C, B2, B5 and B6 content makes bee pollen collected from Rhododendron ponticum plant attractive for diets.
AtıfScopus36WoS27OpenAlex36İncele - MakaleAçık Erişim2021Scopus SJRQ2Turkish royal jelly: amino acid, physicochemical, antioxidant, multi-elemental, antibacterial and fingerprint profiles by analytical techniques combined with chemometrics
Ecem Bayram, Nesrin; Çebi, Nur; Çelik, Saffet; Gerçek, Yusuf Can; Bayram, Sinan; Tanugur Samanci, Asli Elif; Sagdiç, Osman; Özkök, Asli
Royal jelly is one of the most remarkable commercial bee products used in cosmetics, medicine, and as a dietary supplement. This study aimed to determine some quality parameters (amino acid, multi-elemental, total phenolic-flavonoid, 10-HDA, sugar, and moisture content) as well as antioxidant and antimicrobial activities of different royal jelly samples (domestic and non-domestic samples). In this study, royal jelly samples exhibited high antioxidant and antimicrobial activity. 10-HDA values were found to be higher in domestic royal jelly samples (2.17-2.71%) compared to non-domestic samples (0.31-1.71%). Also, it was found that the acidity value varied between 2.57-3.37 mL 0.1 N NaOH/g and 3.87-4.97 mL 0.1 N NaOH/g in non-domestic and domestic royal jelly samples, respectively. L-Proline was the amino acid with the highest rate of occurrence in both domestic (21972.39-35053.00 nmol/g) and non-domestic (19688.31-21711.50 nmol/g) samples. It was determined that the new method developed for amino acid analysis has many advantages over other methods. Chemometrics of principal component analysis (PCA) showed that amino acid profile, elemental profile, and chemical parameters were capable of presenting characteristic and descriptive properties of royal jelly samples. In addition to these findings, Raman spectroscopy combined with chemometrics of hierarchical cluster analysis (HCA) was successfully used to distinguish domestic and non-domestic royal jelly samples. The obtained results will provide an important basis for subsequent research on the evaluation of the amino acid content, physicochemical properties, phenolic content, and the antioxidant and elemental profile of royal jelly.
AtıfScopus24WoS20OpenAlex25İncele - MakaleAçık Erişim2020A mini report on palynological and antibacterial tests of four propolis samples from different regional origins
Bayram, Sinan; Kaci, Fatma Necmiye; Ecem Bayram, Nesrin; Gormez, Arzu
In this study, palynological and antibacterial tests of propolis samples from Iran-Turan (IP1 and IP2),Mediterranean (MP), and Europe-Siberia (EP) phytogeographical regions were performed. The pollens ofApiaceae, Asteraceae, Betulaceae, Boraginaceae, Brassicaceae, Campanulaceae, Caryophyllaceae,Fabaceae, Fagacee, Lamiaceae, Poaceae, Pinaceae, Ranunculaceae, Rosaceae, Salicaceae andScrophulariaceae taxa were found in the palynological analysis. Gram negative and Gram positivebacteria were used to determine in vitro antibacterial activities of the propolis samples. The most potentinhibitory effect against the target microorganisms was obtained from IP1. The most resistant strains wereBurkholderia cepacia, Citrobacter freundii and Streptococcus pneumoniae for all the propolis samples.But, the antibacterial activity levels of the samples were different from each other. These results indicatethat propolis can be assessed in different areas such as cosmetic, medicine and food as an antimicrobialagent.
AtıfOpenAlex2TR Dizin1İncele - MakaleAçık Erişim2020Scopus SJRQ4A study on free-radical scavenging activity, individual phenolic compounds and element concentration of propolis
Ecem Bayram, Nesrin
The aim of this study was to assess the quality of five propolis samples obtained from Turkey (TP), China (CP), Brazil (BP1, BP2), and Ethiopia (EP). The phenolic compounds of the propolis were identified and quantified using the liquid chromatography-tandem mass spectrometry technique (LC-MS/MS). In addition, quality parameters such as total flavonoid content, total phenolic content, free-radical scavenging activity and element contents were investigated. As a result of LC-MS/MS analysis, the extracts were sorted as TP> BP1> BP2> CP> EP in terms of the total concentration of individual phenolic compounds. Chlorogenic acid was determined as the dominant compound in BP1 and EP, p-coumaric acid as the dominant compound in the BP2 and trans-ferulic acid as the dominant compound in the samples from CP and TP. The concentration of DPPH was higher in TP whereas the ABTS concentration was almost similar to other propolis extracts. The contents of potassium (K), calcium (Ca), iron (Fe), magnesium (Mg) and sodium (Na) in the propolis samples were in the range of 2416.75-14416.02 mg/kg, 8.52-613.25 mg/kg, 102.66-1425.82 mg/kg, 523.84-7336.74 mg/kg and 57.65-191.15 mg/kg, respectively. Consequently, it is again supported that chemical characteristics and activity of propolis varies according to its geographical origin with this study.
AtıfOpenAlex3TR Dizin2İncele - MakaleYalnız künye2020Scopus SJRQ2Effects of processing methods and extraction solvents on the chemical content and bioactive properties of propolis
Ecem Bayram, Nesrin; Gerçek, Yusuf Can; Bayram, Sinan; Toğar, Başak
The aim of this study was to evaluate the bioactive properties of propolis extract prepared using different solvents and different extraction methods. The extracts were analyzed by gas chromatography–mass spectrometry (GC–MS) and the differences between their antibacterial activities were evaluated by disc diffusion method. At the same time, bioactive properties of different concentrations of propolis extracts were investigated on human umbilical vein endothelial cells (HUVEC). The proliferative effects and cytotoxic effects of the extracts were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyzes, respectively. Total antioxidant capacity (TAC) and total oxidative status (TOS) parameters were used in assessing biochemical effects in the HUVEC cell line. The DNA damage was also analyzed by 8-oxo-2-deoxyguanosine (8-OHdG) level as indicators of genotoxicity. As a result of the MTT analysis conducted within the scope of the present study, the extracts tested were sorted as 95% ethanol extract of propolis (PEE95) > ultrasonic ethanol extract of propolis (PUEE) > 50% ethanol extract of propolis (PEE50) > ultrasonic water extract of propolis (PUWE) in terms of the effectiveness of their cell viabilities. It was observed that high concentrations of PEE95 induced LDH release. In addition to this, our findings have shown that PEE50, PUEE and PUWE increased oxidative stress at high concentrations. According to 8-OH-dG analysis, all tested extracts were found to be non-genotoxic. The results obtained from antibacterial activity and minimum inhibition concentration tests showed that PUEE and PEE95 had stronger antibacterial effects than PEE50 and PUWE. All these results indicated that propolis has beneficial effects for human health and therefore it is a valuable product which can be used as a food supplement.
AtıfScopus23WoS22OpenAlex25İncele - MakaleYalnız künye2020Scopus SJRQ2Bee bread and bee pollen of different plant sources: determination of phenolic content, antioxidant activity, fatty acid and element profiles
Mayda, Nazlı; Özkök, Aslı; Ecem Bayram, Nesrin; Gerçek, Yusuf Can; Sorkun, Kadriye
This study aims to determine the plant sources, fatty acid composition, total phenolic-flavonoid content, antioxidant capacity, and elemental profile of bee pollen (BP) and bee bread (BB) samples from the same bee hive in different locations. 31 families and 71 species were determined by pollen analysis of BP and BB samples. Pollen frequencies in BB samples were generally similar or less than in BP. Total phenolic varied from 8.26 ± 0.299 to 43.42 ± 0.779 mg GAE/g, and total flavonoid ranged from 1.81 ± 0.040 to 4.44 ± 0.125 mg QE/g. ABTS and DDPH assays indicated that the samples have good antioxidant activity. Samples showed a protein content ranging from 17.6 to 22.2% while the total fatty acid was between 60.27 and 86.49%. The elemental analysis showed that all samples were rich in essential minerals. As a result, total protein, total fatty acids, moisture content and antioxidant capacity of BB samples were found to be lower than those of BP samples from the same hive. In spite of these data, it is necessary to work with more detailed and more samples to be able to say which bee product (bee pollen or bee bread) has superior properties as functional food.
AtıfScopus154WoS143OpenAlex172İncele - MakaleYalnız künye2019Scopus SJRQ3Physicochemical and palynological characterization of the onobrychis miller (fabaceae) honey
Ozenirler, Cigdem; Gencay Celemli, Omur; Ecem Bayram, Nesrin; Dikmen, Fatih; Zare, Golshan; Celikbicak, Omur; Sorkun, Kadriye
The purpose of this study was to contribute toward characterizing the general properties of the Onobrychis Miller (sainfoin) honey.We analyzed 21 samples of Onobrychis honeys, which were collected in 2013, from Northeast Anatolia according to their microscopic and physicochemical features (fructose/glucose (F/G) ratio, 5-(hydroxymethyl) furan-2-carbaldehyde (HMF) value, and volatile compounds). The relationships were examined between these variables with statistical methods such as principal component, regression, and correlation analyses. Statistical analyses revealed certain correlation patterns between several parameters. Onobrychis radiata (Desf.) M. Bieb., O. tournefortii (Willd.) Desv., O. oxyodonta Boiss. and Onobrychis spp. pollens were identified.TPN-10 ranged from 6532 to 481157 while the HMF values were between 0.1-7.5 mg/kg and F/G ratios were between 0.8-1.3. Alcohols, aldehydes, aliphatic acids and their esters, carboxylic acids and their esters, hydrocarbons, flavonoids, ketones, sugars and vitamins were identified in the honey samples within ranges of 0-11.95%, 0-16.76%, 0-24.72%, 0-1.86%, 0-14.71%, 2.74-7.09%, 0-27.02%, 5.12-51.39%, and 0-21.36%, respectively.Although it was not significant, a positive correlation between flavonoids and Onobrychis pollen number was calculated via correlation analysis. There was no meaningful correlation between Onobrychis pollen number and other parameters. Although a negative trend between flavonoids and sugars was observed, it was not significant. F/G ratio was found to be positively correlated with sugars, HMF, and TPN-10 of which the latter two parameters had significant values. Furthermore, a significant positive correlation between TPN-10 and HMF was also detected.
AtıfScopus3İncele - MakaleAçık Erişim2019Melissopalynology analysis, physicochemical properties, multi-element content and antimicrobial activity of honey samples collected from Bayburt, Turkey; [Türkiye'nin Bayburt Ilinden Toplanan Bal Örneklerinin Melissopalinolojik Analizi, Fizikokimyasal Özellikleri, Multi-Element Içerigi ve Antimikrobiyal Aktivitesi]
Ecem Bayram, Nesrin; Yüzer, Mustafa Onur; Bayram, Sinan
The aim of this study was to determine the plant sources, physicochemical properties, element content and antimicrobial effects of honey samples collected from 10 different regions of the province of Bayburt, Turkey. The melissopalynological analysis showed that the pollen samples of 67 plant taxa belonged to 34 plant families in honey samples and were found with different rates and TPN-10 values was found between 16024 and 90126. In addition to these, the amount of ash was between 0.13% and 0.32%, the electrical conductivity value was between 0.36 and 0.69 mS/cm, the moisture content was between 16.1% and 18.9% and the fructose/glucose ratio was between 0.92 and 1.18. As a result of physicochemical analysis, it was determined that the data obtained were in compliance with the standard values defined in by the Turkish Food Codex Communiqué on Honey (No: 2012/58). Elemental analysis performed with ICP-MS showed that the K element was the highest (261.34-1863.05 mg/kg) in all honey samples in total among the 42 elements. In addition to these, the antimicrobial effects of honey samples and minimum inhibition concentration values (MIC) were determined by the agar well diffusion (AWD) method and and microbroth dilution method respectively.
AtıfScopus11OpenAlex12TR Dizin7İncele - MakaleYalnız künye2018Scopus SJRQ2Plant Origins of Propolis from Hakkari, Turkey
Ecem Bayram, Nesrin; Sorkun, Kadriye; Cevahir Oz, Gul
In this study, palynological analysis of 64 propolis samples from Hakkari province in Eastern Anatolia Region of Turkey was carried out. By microscopic analysis method, pollen diagnosis was made in propolis samples, and plants which may be propolis sources, were estimated. As a result, 71 plant taxa belonging to 34 families were identified at different rates in propolis samples. The pollens of the plants belonging to the family Apiaceae (2.7%-35%), Asteraceae (3.4%-35.4%) and, Fabaceae (12%-66%) were considerably found in the propolis of the Hakkari province. This study is the first report introducing the plant profile of Hakkari propolis in detail.
AtıfWoS1İncele - MakaleAçık Erişim2018Scopus SJRQ3Melissopalynological Analysis for Geographical Marking of Kars Honey
Gençay Çelemli, Omur; Özenirler, Cigdem; Ecem Bayram, Nesrin; Zare, Golshan; Sorkun, Kadriye
In this research, the melissopalynological analysis of honey samples collected from Kars city located in the East Anatolian Region of Turkey was conducted for geographical marking. Within this context, melissopalynological analyses of 100 honey samples determined by sampling method were collected from eight districts of Kars in Eastern Anatolia Region of Turkey were done, to determine the nectarous source plants of Kars honey. As a result of melisopalynological analyses carried out in 100 honey samples; pollens of the taxa belonging to Apiaceae, Asteraceae, Berberidaceae, Betulaceae, Brassicaceae, Boraginaceae, Campanulaceae, Caryophyllaceae, Chenopodiaceae, Cistaceae, Cyperaceae, Dipsacaceae, Ericaceae, Fabaceae, Iridaceae, Lamiaceae, Liliaceae, Malvaceae, Onagraceae, Papaveraceae, Plantaginaceae, Poaceae, Polygonaceae, Ranunculaceae, Rhamnaceae, Rosaceae, Rubiaceae, Rutaceae, Salicaceae and Scrophulariaceae families were detected at different rates. Almost in all of the honey samples, Lotus corniculatus (in 99 samples), Onobrychis radiata (in 99 samples), Trifolium nigrescens (in 88 samples) from Fabaceae family and pollens of Echium vulgaris (81 samples) and Myosotis lithoospermifolia (15 samples) taxa from the Boraginaceae family, were found in honey samples. Onobrychis radiata pollen was the most intensely observed one among these samples (in dominant, secondary, minor, trace amounts). The total number of pollens (TPN-10) in 10 grams of honey were also detected during the melissopalynological analyses. TPN-10 values minimum: 226, maximum: 481157 and mean: 31678 were detected and the pollen abundance of the honeys are classified as good category. Kars is an important province for beekeeping with floral variety. As a result of this study, the first step of the geographical marking studies of Kars' honey was completed.
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