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- MakaleAçık Erişim2025Scopus SJRQ2Biosynthesis of Functionalized Silver Nanomaterials Utilizing Seeds of Black Rosehip (Rosa pimpinellifolia L.) and its Remarkable Antioxidative Capacity
Kizil, Hamit Emre
Background: Silver nanoparticles (AgNPs) have gained significant attention due to their unique antimicrobial, catalytic and optical properties. This study focuses on the green synthesis of silver nanoparticles using black rosehip (Rosa pimpinellifolia L.) seeds (Rp-AgNPs) and evaluates their biological activities. Methods: Rp-AgNPs were synthesized using R. pimpinellifolia seed extract and characterized using multiple analytical techniques including UV-Vis spectroscopy, XRD, FTIR, TEM, SEM, EDAX, zeta potential analysis, DSC, SERS and XPS. The biological activities were assessed through DPPH free radical scavenging assay, cytotoxicity testing against H460 lung cancer cells and antimicrobial activity evaluation against various pathogens. Result: The synthesized Rp-AgNPs exhibited spherical morphology with an average size of 51.27 nm and demonstrated stability with a zeta potential of-18.9. The nanoparticles showed enhanced antioxidant activity (IC50 = 30.89 μg/mL) compared to the seed extract (IC50 = 136.4 μg/mL). Cytotoxicity studies revealed significant effects against H460 cells with an IC50 of 65.8 μg/mL. Antimicrobial testing demonstrated effectiveness against both Gram-positive and Gram-negative bacteria (MIC 50-100 μg/mL) and Candida strains (MIC 100-200 μg/mL).
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İncele - Yüksek Lisans TeziAçık Erişim2022Sülfonil hidrazon türevlerinin sentezi ve biyolojik aktivitelerinin incelenmesi
Yasin Demirci
Hidrazonlar, kimyasal reaktiviteleri ve sentetik çalışmaları ile 1894 yılından bu yana modern yöntemlerle üstünde kapsamlı bir şekilde çalışılan önemli organik bileşik gruplarıdır. Organik sentez, biyokimya, antitümöral ve antimikrobiyal gibi çeşitli biyolojik alanlarda olduğu kadar supramoleküler kimya gibi analitik alanlarda da önemli aktiviteleri vardır. Hidrazon türevleri, organik kimyada çok yönlü bir bileşik sınıfını oluşturur. Bu yapılar birbirine bağlı iki nitrojen atomu ve terminal nitrojen atomunun yalnız elektron çiftiyle konjuge edilmiş bir C=N çift bağını içerir. Sülfonil hidrazonlar ise sülfonil hidrazit türevlerinin, sentetik olarak elde edilen açillenmiş aldehit bileşikleri ile asit katalizli kondenzasyon reaksiyonu sonucunda elde edilmektedir. Sülfonil hidrazonlar enzimatik modülasyon, analjezik, antifungal ve potansiyel antibakteriyel gibi farmakolojik etkileri ile iyi bilinirler. Buna ilaveten, anti-alzheimer, antidepresan, antineoplastik aktivite ve antidiyabetik ajanlar olarak da hareket edebilirler. Bu tez çalışmasında yapısında para pozisyonunda brom, flor ve metoksi gibi substituentler ihtiva eden aromatik sülfonil klorür bileşikleri düşük sıcaklıkta hidrazin reaktifi ile muamele edilerek 4 farklı sülfonil hidrazit bileşiği elde edilecektir. Daha sonra bu sülfonil hidrazit türevleri, sentetik olarak vanilin bileşiğinden elde edilen açillenmiş aldehit bileşikleri ile asit katalizli kondenzasyon reaksiyonuna tabi tutulacaktır. Bu reaksiyon sonucunda 15 tane hedef bileşiğin sentezi gerçekleştirilmiş olacaktır. Ayrıca 1H-NMR, 13C-NMR, FT-IR ve HRMS gibi spektroskopik analizler kullanılarak bu hedef bileşiklerinin yapısı aydınlatılacaktır. Daha sonra bu bileşikler biyolojik uygulamalar için kullanılacaktır. Anahtar Kelimeler: Hidrazon bileşikleri, sülfonil hidrazon türevleri, biyolojik aktivite, antioksidan, heterosiklik bileşik
- MakaleAçık Erişim2022Scopus SJRQ3Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits
Şahin, Birgütay; Bülbül, Ali Savaş; Çelik, I. Seyfettin; Korkmaz, Nesrin; Karadağ, Ahmet
In this study, biological activities of silver nanoparticles (AgNP) synthesized by green synthesis method using the fruit extract of Gilaburu (Viburnum opulus L.) plant were investigated. The characterization of the synthesized AgNPs was performed by ultraviolet visible region spectroscopy (UV-Vis), Fourier transform - infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques. The crystal size was found to be 52.32 nm when the XRD data were calculated with the Debye-Scherrer's equation. The antimicrobial activities of extract and AgNPs were investigated by microdilution and disk diffusion methods. Antibiofilm activities were examined by the crystal violet technique. The cytotoxic effects of the extract and AgNPs against MCF-7 (human breast cancer) and HUVEC (human umbilical vein endothelial cells) cell lines were evaluated by MTT assay. The IC50 values for the HUVEC line were found to be 0.97 mg/mL (AgNP) and 85.24 mg/mL (extract), while the IC50 values for the MCF-7 line were determined as 0.022 µg/mL (AgNP) and 0.021 µg/mL (extract). To our knowledge, this is the first report to comprehensively examine the biological activities of V. opulus L. extract and biosynthetic AgNPs.
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İncele - MakaleYalnız künye2017Scopus SJRQ4Bioactive content of Rosa canina biotypes from Turkey
Beyhan, O.; Koc, A.; Ercisli, S.; Jurikova, T.; Cakir, O.
Rose hips have been used as medicinal and aromatic plants for a long time throughout Turkey. The plant has been the focus of many recent studies due to its potential effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa canina biotypes growing wild in Sivas province located eastern Anatolia. The results revealed that fruits of Rosa canina biotypes rich in terms of Vitamin C which ranged from 360 to 482 mg/100 g fresh weight base. The total phenolic content varied from 340 to 464 mg/100 g fresh weight. The biotype S-07 had the highest total flavonoids content (241 mg quercetin equivalent/100 g fresh weight) and the lowest value attributed to biotype S-01 as 151 mg/quercetin equivalent/100 g fresh weight. The antioxidant activity of the biotypes was between 14.2-30.7 μg Trolox/ml sample. The results revealed that there was enough diversity among Rosa canina biotypes for bioactive content and promising biotypes with high bioactive content are ready material for future breeding activities.
AtıfScopus4İncele - MakaleAçık Erişim2016Scopus SJRQ2Bioactive content of rose hips of different wildly grown rosa dumalis genotypes
Alp, Sevket; Ercisli, Sezai; Jurikova, Tunde; Cakir, Ozlem; Gozlekci, Sadiye
A rose hip is the fruit of a rose plant and mostly belongs to Rosa canina and Rosa dumalis. These species are genuine found as wild in natural conditions, while in some countries are cultivated for their hips that are picked and used in a wide variety of preparations. Because they contain a variety of antioxidants, carotenoids, flavonoids, polyphenols, leucoanthocyanins and catechins, rose hips are considered to be a good cancer preventative. The Rosa genus has been the focus of many recent studies due to its potential benefic effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content, including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa dumalis genotypes growing wild in Erzurum province located eastern Anatolia. The results revealed that fruits of Rosa dumalis different genotypes were rich rich in terms of vitamin C, which ranged from 402 to 511 mg/100 g fresh weight base. The total phenolic content varied from 297 to 403 mg/100 g fresh weight. The genotype 'E-09' had the highest total flavonoids content (229 mg quercetin equivalent/100 g fresh weight) and the lowest value was attributed to genotype 'E-04' with 143 mg/ quercetin equivalent/100 g fresh weight. The antioxidant activity of the genotypes was between 12.9-28.6 μg Trolox/ml samples. The results revealed that there was enough diversity among Rosa dumalis genotypes for bioactive content and promising genotypes, with high bioactive content, were determined, which can become study material for future breeding activities.
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