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- MakaleAçık Erişim2022Scopus SJRQ3Investigation of the recovery efficiency of CeUF method through RT-qPCR quantification of inactivated SARS-CoV-2 in untreated wastewater
Sait, Ahmet; Korkmaz, Serol; Parmaksiz, Ayse; Bayraktar, Bulent
Wastewater-based disease monitoring is an early warning system and a surveillance tool for infectious disease outbreaks regarding pathogens with pandemic potential. This study aimed at investigating the recovery efficiency of centrifugal ultrafiltration (CeUF), which is one of the most-used virus concentration methods, for inactive severe acute respiratory syndrome coronavi-rus 2 (SARS-CoV-2) added to wastewater. Inactivated SARS-CoV-2 was inoculated into untreated wastewater at different concentrations (4 × 103, 8 × 103, 16 × 103, 24 × 103 and 32 × 103 gene copy/ µL) and concentrated through ultrafiltration with a disposable centrifugal filter device. Total nucleic acids in concentrated filtrates were extracted and isolated by an automated system. In isolates, total RNA was measured by a UV/VIS spectrophotometer, and the recovered virus was quanti-fied by RT-qPCR with two gene regions (N1 and N2). The recovery rates were between 11% and 17.8% (mean 15.1%, CV below 15%). While there were positive correlations among the inoculated virus, total RNA and recovered virus, there was no correlation and linearity between the recovery rates. Despite limited recovery rates, CeUF integrated with RT-qPCR quantification can be a valid assay for monitoring SARS-CoV-2 in wastewater, and an early warning system.
AtıfScopus2WoS2OpenAlex3
İncele - MakaleAçık Erişim2021Scopus SJRQ3A novel weighted mean-squared error optimization model to obtain optimal conditions of adsorption factors for a lead removal process
Özdemir, Akın; Teke, Çağatay; Serencam, Hüseyin; Uçurum, Metin; Gündoğdu, Ali
Lead (Pb) removal process from wastewater is an important issue to prevent health problems for people. For this particular purpose, a low-cost adsorbent may be beneficial for improving the adsorption capacity for the Pb removal process. The aims of this paper are four-fold. First of all, a D-optimal experimental design was selected to reduce experimental runs and its cost. Second, the effect of four adsorption design factors, stirring speed (rpm), adsorbent dosage (g), pH level, and initial metal concentration (ppm), was examined. Also, the yellow natural stone, which is from Bayburt, Turkey, was used as a cheap adsorbent for the Pb removal process from the solu-tion. Third, a novel weighted mean-squared error optimization model was developed to obtain optimal adsorption levels for adsorption factors. Besides, a verification study was conducted to verify the results of the adsorption experiment. Finally, the lead (Pb(II)) removal capacity of the yellow Bayburt stone was obtained to be 46.031 mg/g, and the results of the experiment from the proposed methodology showed a good performance for the removal study.
- MakaleAçık Erişim2019Scopus SJRQ2Modeling design parameters with taguchi experimental method for obtaining operating conditions for cu(Ii) removal through adsorption process
Serencam, Hüseyin; Özdemir, Akın; Teke, Çağatay; Uçurum, Metin
One of the environmental concerns deals with the removal process of pollutants in water and wastewater. In this paper, a removal process was used to eliminate pollutants in water and wastewater. The aim of this paper was to obtain an optimum adsorption condition for the highest metal ions adsorbed (MIA) mg/g. For this particular purpose, a Taguchi L18 design was conducted. In addition, the signal-to-noise ratio was analyzed for optimum adsorption levels. The analysis of variance was also performed to evaluate the effect of each adsorption condition on MIA (mg/g) values. Moreover, an optimization model was also proposed to find the best optimal setting of adsorption levels. Then, the confirmation tests were performed using optimum coded levels of the adsorption parameters for the verification purpose. The results of the experimental study showed a good performance that Cu(II) removal capacity was found to be 43.66 mg/g. To the best of our knowledge, this research is the first to study natural stone as an efficient, inexpensive and cheap adsorbent for the removal process.
AtıfScopus6WoS5OpenAlex6İncele - MakaleAçık Erişim2018Scopus SJRQ2Development of cloud point extraction preconcentration of cadmium and lead in solid samples using flame atomic absorption spectrometry
Bahadır, Zekeriyya; Yazar, Murat; Tümay, Süreyya Oğuz; Serencam, Hüseyin; Bektaş, Hakan; Duran, Celal
2-[(6-Morpholin-4-ylpyridin-3-yl)amino]-N’-(4-oxo-3-phenyl-1,3thiazolidin-2-ylidene) acetohydrazide (MPAPTAH) was first used in a cloud point extraction procedure to determine the cadmium and lead ions in solid samples. Triton X-114 was used as a mixed micellar medium. The optimal extraction parameters (e.g. surfactant concentrations, amount of ligand, pH etc.) were evaluated. The optimum experimental conditions for Cd and Pb ions during the extraction procedure were carried out with 0.7 mg of MPAPTAH agent, 1.0 mL 0.2% (w/v) of the surfactant, a pH of 8.0. Under the best analytical parameters, the detection limit (LOD) for Cd(II) and Pb(II) ions were found to be 0.6 μg L−1 and 1.91 μg L−1, while the relative standard deviation (RSD) was taken as 2.78 and 2.86%, respectively. Preconcentration factor (PE) of 50 was obtained from using 50 mL of the sample volume for both ions. The accuracy of the method was tested through analysis of Pb2+ and Cd2+ in certified reference material (CRM Sandy Soil C). The interference effect of certain cations and anions were also investigated. The recoveries of the analyte ions were almost quantitative in the presence of all the interfering ions. Afterwards, the developed method was successfully applied to find lead and cadmium in real solid samples, including black tea, tobacco, and chili pepper.
AtıfScopus9WoS8OpenAlex8İncele - DergiDesalination and Water Treatment