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- MakaleAçık Erişim2025Scopus SJRQ1Sensitive and selective nitrite sensor based on CuCo2O4-rGO nanocomposite synthesized by one-pot electrochemical method in pastırma
Anlar Daldal, Pınar; Öztürk Doğan, Hülya; Cinar Topcu, Kübra
In this study, CuCo₂O₄-rGO nanostructures were synthesized by a one-pot electrochemical method on the surface of Pencil Graphite Electrode (PGE) for nitrite determination in pastırma, a traditional meat product. The developed modified electrode was used to determine the amount of nitrite in pastırma samples obtained from three different brands in the Turkish market. When the nitrite values obtained by the electrochemical method were compared with the spectrophotometric method, they showed 91.20 % to 100 % agreement. In addition, the physicochemical properties of pastırma samples, such as pH, moisture, protein, fat, and ash, were analysed, and it was determined that pH and moisture values are critical parameters for product quality. These results show that the developed electrode provides a practical and sensitive method for nitrite sensing.
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İncele - MakaleAçık Erişim2021Potential bacteriocinogenic lactic acid bacteria from pastırma; [Pastırmadaki potansiyel bakteriyosinojenik laktik asit bakterileri]
Güllüce, Medine; Kaya, Mükerrem; Kaban, Güzin; Karadayi, Mehmet; Alaylar, Burak; Kamiloğlu, Aybike; Işik, Ceyda; Fettahoğlu, Kübra
Recent researches conducted have focused on the possibility of using bacteriocin produced by lactic acid bacteria both (LAB) as protective cultures and as natural antimicrobial agents in dry cured meat products such as pastırma. In the present study, 50 lactic acid bacteria strains, previously isolated from various traditional pastırma samples, were tested for their antimicrobial potentials. Determination of antagonistic activities against food-borne pathogenic strains (Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus) were done by using agar-spot and well diffusion assays. Then, active isolates were identified by using 16S rRNA gene sequence analysis. According to the results, 11 of tested LAB were determined with significant bacteriocinogenic potential and these were assigned to as 1 Lactobacillus plantarum, 9 L. plantarum subsp. plantarum and 1 Pediococcus pentosaceus. In conclusion, it was reached that the isolated/identified bacteriocin-producing lactic acid bacteria strains from pastırma samples have a significant potential to prepare pure or crude bacteriocin preparations.
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İncele - MakaleYalnız künye2020Scopus SJRQ1Probiotic properties of lactic acid bacteria strains isolated from pastırma
Topçu, Kübra Cinar; Kaya, Mükerrem; Kaban, Güzin
In this study, the probiotic properties of 80 lactic acid bacteria (LAB) strains isolated from pastırma, a dry-cured meat product, were investigated. For this purpose, strains were investigated for their resistance to bile salt and hydrophobicity abilities. In 6 strains selected as a result of these analyses, development at high salt concentrations, auto-aggregation and co-aggregation, resistance to low pH, and adhesion analyses were performed. The growth percentage of all strains decreased with increasing bile salt concentration at both 25 °C and 37 °C. P. pentosaceus K7, K41, K44, K51 and K81 and P. acidilactici K99 gave the highest values in terms of hydrophobicity ability. The highest auto-aggregation and the highest co-aggregation were found in P. pentosaceus K41 (17.62%) and P. pentosaceus K44 strain (26.23%), respectively. These six strains showed tolerance to high acidity even at pH 2. The strains significantly maintained their viability in the simulated gastric and intestinal fluids. Furthermore, the strains had low adhesion capacity in Caco-2 and HT-29 cell lines and generally showed resistance under gastrointestinal system conditions. As a result, P. pentosaceus K44, P. acidilactici K99 and P. pentosaceus K41 could be considered as probiotic strains.
AtıfScopus55WoS48OpenAlex50İncele - MakaleAçık Erişim2019Scopus SJRQ3Genotypic identification of lactic acid bacteria in pastirma produced with different curing processes; [Farklı kürleme İşlemleri İle üretilen pastırmada laktik asit bakterilerinin genotipik İdentifikasyonu]
Çınar, Kübra; Fettahoğlu, Kübra; Kaban, Güzin
The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two different curing temperatures (4°C or 10°C) and two different curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identification. According to the identification results, 68 of 87 isolates (78.16%) was identified as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L. plantarum (2.30%), respectively. P. pentosaceus was dominant species in all curing applications (4°C/nitrate or nitrite or 10°C/nitrate or nitrite). Another species determined in all groups was P. acidilactici. While L. plantarum was only isolated from samples produced with nitrate (4°C or 10°C), L. sakei was isolated from samples produced with nitrite (4°C or 10°C). The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effective than the curing temperature.
AtıfScopus3WoS3OpenAlex2İncele - MakaleAçık Erişim2019Scopus SJRQ3Biodiversity and characterization of gram-positive, catalase-positive cocci isolated from pastırma produced under different curing processes
Fettahoğlu, Kübra; Çinar, Kübra; Kaya, Mükerrem; Kaban, Güzin
The aim of this study was to determine the effects of different curing temperatures (4 °C or 10 °C) and agents (KNO3 or NaNO2) on microbiological and physicochemical properties of pastırma and diversity of gram-positive, catalase-positive cocci (GCC+) and also to investigate some technological properties of the strains. Four different groups of pastırma were produced applying different curing temperatures and agents. After production, GCC+ isolated from pastırma were subjected to phenotypic and genotypic (16S rRNA sequencing) identification. Genotypically characterized strains were evaluated for their technological properties. In all groups, aw and pH were observed to be under 0.90 and over 5.5, respectively. In genotypic identification, 45 isolates were identified as Staphylococcus vitulinus, while other isolates identified were S. equorum (n = 15), S. saprophyticus (n = 15), and S. xylosus (n = 12). All of the strains could grow at different salt concentrations (8% and 15%) and temperatures (4 °C, 10 °C, and 37 °C). Four strains of S. saprophyticus showed strong lipolytic activity while nine strains of S. vitulinus had strong proteolytic activity. This study showed that different curing temperatures and agents are particularly effective on the microflora in pastırma; however, these factors do not cause a high diversity in GCC+ in terms of species.
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