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- MakaleAçık Erişim2026Scopus SJRQ1Effects of graviola oil extract on fattening performance and circadian rhythms of adipokine, cardiac and mitochondrial function markers in lambs
Kotan, Gökşad Cemil; Bayraktar, Bülent
Background: This study investigated the effects of dietary graviola (Annona muricata L.) oil extract (GOE) (supplemented at 200, 400, and 600 mg/kg) on the growth performance and circadian rhythm profiles of adipokine, cardiac, and mitochondrial function biomarkers in 48 male Anatolian Merino lambs (2.5 months old). Methods: Lambs were assigned to a control group (G0) or supplementation groups (G200, G400, G600 mg/kg) for a 60-day trial (70 days total). The study was conducted under a 12-h light/12-h dark cycle to ensure controlled environmental conditions. To analyze circadian profiles, blood samples were collected on days 0, 15, 30, 45, and 60 at four time points (07:00, 13:00, 19:00, and 01:00). Serum levels of Apelin, cTnI, BMAL1, MOTS-c, and BMCP1 were measured by ELISA. Data were analyzed using GLM Multivariate analysis (p < 0.05). Results: Key findings showed the G400 dose resulted in the most effective linear increase in live weight and live weight gain by day 60, and had a significant overall effect on live weight (p < 0.05). The G200 dose significantly improved the feed conversion ratio (p < 0.05), while feed consumption was unaffected (p > 0.05). Regarding biomarkers on day 60, the G400 dose effectively modulated BMAL1, MOTS-c, and BMCP1 (peak at 19:00). The G600 dose yielded the best results for Apelin (peak at 19:00) and cTnI (peaks at 13:00 and 07:00). Conclusions: In conclusion, GOE at a dose of 400 mg/kg (G400) not only optimizes fattening performance in Anatolian Merino lambs but also supports metabolic homeostasis by modulating adipokine levels, cardiac function, and mitochondrial biogenesis processes in a manner consistent with the circadian rhythm. These findings demonstrate that the G400 dose is a safe and effective phytogenic strategy for improving animal health and production efficiency.
- MakaleAçık Erişim2024Scopus SJRQ3The Relationship of Mitochondrial-derived Peptide (MOTS-c) and Brain Mitochondrial Carrier Protein 1 (BMCP1) Response in Sheep with Some Physiological Parameters
Bayraktar, B.; Demir, N.; Aksakal, V.
Background: Mitochondria are organelles called power plants of the cell, which are found in eukaryotic cells and are responsible for the production of energy needed by the cells through aerobic respiration. Mitochondrial dysfunction causes endocrinological, cardiovascular, metabolic and neurodegenerative diseases. The aim of this study was to examine the effects of body condition score (BCS), lactation, pregnancy, age and gender on Mitochondrial-Derived Peptide (MOTS-c) and Brain Mitochondrial Transporter Protein 1 (BMCP1) levels in Akkaraman sheep. Methods: Sheep are classified into 3 categories according to BCS (BCS<2, BCS=3-3.5, BCS≥4). In creating groups in terms of BCS, it was determined by the palpation method applied to the waist area. The sheep included in the study were divided into 4 groups: early lactation, early pregnancy, late pregnancy (dry period) and age (female and male sheep).1st group, ewes in the first period of pregnancy (on the 100th day of the first pregnancy) (n= 30), 2nd group, ewes in the early lactation period (n= 30), 3rd group, ewes in the dry period (the last two months of pregnancy) (n= 30) and in the 4th group, <2 (young) (n=30), 2-8 (adult) (n=30), 8> (old) (n=30), male and female sheep (n=30) of different ages. =180) total of 270 animals. Blood samples were taken from the jugular vein of the sheep before the morning feeding. MOTSc and BMCP1 levels were determined in blood serum samples by ELISA method. Result: In the study, serum MOTS-c and BMCP1 levels in sheep during lactation and pregnancy were determined to be lowest in the VKS<2 group and highest in the VKS ≥4 groups. Likewise, in age and gender groups, the highest level was detected in young lambs and yearlings in the VKS<2 group, while the highest level was detected in old sheep and rams in the VKS ≥4 group. As a result, it was determined that different age, gender, pregnancy and lactation periods affected serum MOTS-c and BMCP1 levels depending on the change in BCS (P<0.05). It was concluded that MOTS-c and BMCP1 may be useful parameters in the evaluation of mitochondrial function, energy metabolism and metabolic profile.
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