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- MakaleYayıncıda açık erişim2025Scopus SJRQ4ANALYSIS OF PESTICIDES, ANTIBIOTICS, AND HEAVY METAL LEVELS IN HONEY PRODUCED IN THE BAYBURT AND UPPER ÇORUH VALLEY REGIONS OF TÜRKİYE; [Türkiye'nin Bayburt ve Yukarı Çoruh Vadisi Bölgelerinde Üretilen Ballarda Pestisit, Antibiyotik ve Ağır Metal Düzeylerinin Analizi]
Özdemir, Mustafa; Yildizlar, Osman
This study investigates pesticide, antibiotic, and heavy metal levels in Bayburt and the Upper Çoruh Valley honey. Thirteen honey samples were collected from different apiaries managed by stationary and migratory beekeepers. These samples were analysed for heavy metals (Fe, Cu, Zn, Cd, Pb), antibiotics, pesticides, and chemical residues such as naphthalene. The results revealed that lead (Pb) levels exceeded international food safety standards in 2 honey samples, while pesticide residues were detected in 5 samples. Additionally, antibiotic residues were found in 6 samples, including sulfamethazine, tetracycline, and streptomycin. However, no naphthalene was detected in any of the samples. These findings highlight the importance of stricter regulations and monitoring systems to control chemical use in beekeeping practices. Enhancing awareness among beekeepers regarding the risks associated with pesticide and antibiotic use is crucial for improving honey quality and ensuring the health of beekeepers and consumers. The adoption of safer practices and adherence to guidelines are necessary to mitigate these health hazards.
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İncele - MakaleAçık Erişim2015Scopus SJRQ2The toxicological impacts of some heavy metals on carbonic anhydrase from gilthead sea bream (Sparus aurata) gills
Kaya, Elif Duygu; Söyüt, Hakan; Beydemir, Şükrü
It is known that heavy metals have toxic effects on fish. Insufficient measures are a serious problem in our country and around the world. This problem can threaten human health in areas where it is common for people to obtain nutrition from local bodies of water. In this study, the toxicological impacts of some heavy metals were investigated on carbonic anhydrase activity in gilthead gills. Carbonic anhydrase (CA) was purified from gilthead sea bream (Sparus aurata) gills with a specific activity of 2872.92EUmg-1 and a yield of 32.84% using affinity chromatography. The overall purification was approximately ~84-fold. SDS-polyacrylamide gel electrophoresis showed a single band, and the MW was approximately 30.5kDa (Soyut et al., 2008, 2012; Soyut and Beydemir, 2008, 2012; Kaya et al., 2013). The kinetic and characteristic properties of CA such as the optimum pH, stable pH, optimum temperature, activation energy (Ea), activation enthalpy (δH), Q10, Km and Vmax were determined. Cadmium (Cd2+), copper (Cu2+), nickel (Ni2+) and silver (Ag+) inhibited CA activity in in vitro conditions. Ki values were calculated for these metals. Ki values were 31.20mM for cadmium (Cd2+), 161.96mM for copper (Cu2+), 10.79mM for nickel (Ni2+) and 0.0082mM for silver (Ag+) based on Lineweaver-Burk plots. Except for cadmium, heavy metals had the same inhibition mechanism. Cadmium was competitive, and the others were noncompetitive.
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İncele - MakaleAçık Erişim2015Scopus SJRQ2Simultaneous separation and preconcentration of Cd(II), Co(II), and Ni(II) ions in environmental samples by carrier element-free coprecipitation method prior to their flame atomic absorption spectrometric determination
Duran, Celal; Ozdes, Duygu; Akcay, Hakki Turker; Serencam, Huseyin; Tufekci, Mehmet
ABSTRACT: In the present study, Cd(II), Co(II), and Ni(II) ions were separated and preconcentrated via carrier element-free coprecipitation (CEFC) method by using an organic coprecipitating agent, 1,2-bis-(2-tosyl ethoxy) benzene (BTEB), prior to their flame atomic absorption spectrometric (FAAS) detections. The experimental conditions were optimized in terms of pH of the aqueous solution, BTEB amount, standing time, centrifugation rate and time, and sample volume. Under the optimum experimental conditions, the detection limits for Cd(II), Co(II), and Ni(II) ions were found to be 0.13, 0.67, and 0.46 μg L−1, respectively, and the relative standard deviations for the analyte ions were found to be lower than 4.0%. The accuracy of the method was tested by analyzing certified reference material and spike tests. The method was applied to determine the levels of Cd(II), Co(II), and Ni(II) ions in sea and stream water, macaroni, red lentil, and cracked wheat samples.
AtıfScopus17WoS16OpenAlex16İncele - MakaleAçık Erişim2014Scopus SJRQ1Simultaneous separation and preconcentration of Ni(II) and Cu(II) ions by coprecipitation without any carrier element in some food and water samples
Duran, Celal; Tumay, Sureyya O.; Ozdes, Duygu; Serencam, Huseyin; Bektas, Hakan
A simple and highly sensitive separation and preconcentration procedure, which has minimal impact on the environment, has been developed. The procedure is based on the carrier element-free coprecipitation of Ni(II) and Cu(II) ions by using 2-{4-[2-(1H-Indol-3-yl)ethyl]-3-(4-chlorobenzyl)-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl}-N′-(pyrrol-2-ylmethyliden)acetohydrazide (ICOTPA), as an organic coprecipitant. The levels of analyte ions were determined by flame atomic absorption spectrometry. The detection limits for Ni(II) and Cu(II) ions were found to be 0.27 and 0.58 μg L-1, respectively, and the relative standard deviations for the analyte ions were lower than 4.0%. Spike tests and certified reference material analyses were performed to validate the method. The method was successfully applied for the determination of Ni(II) and Cu(II) ions levels in sea and stream water as liquid samples and red lentil and rice as solid samples.
AtıfScopus26WoS23OpenAlex26İncele - MakaleAçık Erişim2013Scopus SJRQ2Carbonic anhydrase activity from the gilthead sea bream (Sparus aurata) liver: The toxicological effects of heavy metals
Kaya, Elif Duygu; Söyüt, Hakan; Beydemir, Sukru
Many studies have shown that metal ions may lead to oxidative stress in biological systems. Accordingly, DNA damage, protein modification, enzyme inhibition and activation, lipid peroxidation and many other effects may occur in living organisms. Many different formations of metal ions may enter human cells along with water, air, and various foods, and humans are negatively affected by these conditions, either directly or indirectly. These effects may cause irreversible damage to human metabolism. In this study, the toxicological effects of heavy metals on carbonic anhydrase enzyme activity from the gilthead sea bream liver were investigated. The carbonic anhydrase enzyme was purified via affinity chromatography and had a specific activity of 6775.5 EU mg(-1). The kinetics and characteristic properties, such as optimum pH, stable pH, optimum temperature, activation energy (E-a), activation enthalpy (Delta H), Q(10), K-m, and V-max, were determined for the purified enzyme SDS-polyacrylamide gel electrophoresis showed a single band and molecular weight of the subunit was approximately 25 kDa. Cd(II), Cu(II), Ni(II) and Ag(I) inhibited the enzyme activity in vitro. The type of inhibition and K-i values for these metals were calculated from Lineweaver-Burk plots as 17.74 mM, 36.20 mM, 12.85 mM and 0.025 mM for Cd(II), Cu(II), Ni(II) and Ag(I), respectively. All the metals were noncompetitive inhibitors. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
AtıfWoS27OpenAlex32İncele - MakaleYalnız künye2013Scopus SJRQ3Application of carrier element free coprecipitation (CEFC) method for determination of Co(II), Cu(II) and Ni(II) ions in food and water samples
Serencam, Huseyin; Duran, Celal; Ozdes, Duygu; Bektas, Hakan
A simple and highly sensitive separation and preconcentration procedure, which has minimal impact on the environment, has been developed. The procedure is based on the carrier element free coprecipitation (CEFC) of Co(II), Cu(II), and Ni(II) ions by using 2-{4-[2-(1H-indol-3-yl)ethyl]-3-(4-methylbenzyl)-5-oxo- 4,5-dihydro-1H-1,2,4-triazol- 1-yl}-N'-(pyridin-2-yl methylidene)acetohydrazide (IMOTPA), as an organic coprecipitant. The levels of analyte ions were determined by flame atomic absorption spectrometry (FAAS). The detection limits for Co(II), Cu(II) and Ni(II) ions were found to be 0.40, 0.16 and 0.17 μg L-1, respectively, and the relative standard deviations for the analyte ions were lower than 3.0%. Spike tests and certified reference material analyses were performed to validate the method. The method was successfully applied for the determination of Co(II), Cu(II) and Ni(II) ions levels in sea and stream water as liquid samples and red pepper, black pepper, and peppermint as solid samples.
AtıfScopus10WoS10İncele - MakaleAçık Erişim2012Scopus SJRQ3Changes in carbonic anhydrase activity and gene expression of Hsp70 in rainbow trout (Oncorhynchus mykiss) muscle after exposure to some metals
Söyüt, Hakan; Beydemir, Sükrü; Ceyhun, Saltuk Buǧrahan; Erdoǧan, Orhan; Kaya, Elif Duygu
The effects of some heavy metals Co(II), Cu(II), Zn(II), and Ag(I) on carbonic anhydrase (CA) activity from rainbow trout (RT) muscle were investigated. Moreover, the effects of these metals on the expression of heat shock protein 70 (Hsp70) gene in muscle tissue were examined by real-time quantitative PCR (RT-PCR) in muscle tissue aft er exposure to the metals at the end of 6, 12, 24, and 48 h. CA was purified with a specific activity of 2300 EU mg-1, a yield of 19%, and 1080-fold. The molecular weights (Mw) of subunit and native enzyme were approximately 30 and 31 kDa, respectively. Optimum pH, stable pH, optimum temperature, activation energy (Eα), activation enthalpy (ΔH), and Q10 (the difference in activity of enzyme caused by the increment of 10 oC) value were determined. Apparent Michaelis constant (Km), maximum reaction rate (Vmax), and turnover rate of the enzyme (Kcat) values were 1.29 mM, 0.17 μmol min-1, and 28.8 s-1, respectively. The catalytic efficiency (Kcat/Km) was 22.3. The heavy metals decreased in vitro CA activity. Inhibition mechanisms of the metal ions were noncompetitive, except for the Co(II) ion, which was competitive. The expression of the Hsp70 gene was increased in the presence of the metal ions. The expression level at the end of 48 h was the highest for all of the metals. Consequently, the in vitro inhibition rank order was determined as Co(II) > Zn(II) > Cu(II) > Ag(I). Interestingly, Ag(I) was the most effective metal ion on Hsp70 gene expression.
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