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- MakaleAçık Erişim2026Scopus SJRQ2Production of Gamma-Aminobutyric Acid by Levilactobacillus brevis AK19B: Strain Characterization and Process Optimization Using Box–Behnken Design
Memiş, Habibe; Kamiloğlu, Aybike
Levilactobacillus brevis AK19B strain demonstrated promising probiotic potential as a hydrophilic lactic acid bacterium with valuable phytase activity. It also exhibited strong antioxidant properties and maintained viability in bile salt-rich environments, suggesting robustness in gastrointestinal conditions. To maximize gamma-aminobutyric acid (GABA) production, a Box–Behnken design was applied to assess the effects of monosodium glutamate (MSG) concentration, NaCl concentration, pH, and temperature. The results showed that these factors significantly influenced GABA synthesis, with their interactions well-modeled by a quadratic response surface. Optimal conditions were identified as pH 5.136, MSG concentration of 297 mM, temperature at 26 °C, and NaCl concentration of 2.82%, under which the predicted GABA production reached 43.85 mg/mL. These findings highlight AK19B as a promising candidate for functional food applications and industrial GABA biosynthesis.
- MakaleAçık Erişim2025Scopus SJRQ1The impact of osmotic dehydration and microwave drying process conditions on the quality characteristics of celery roots (Apium graveolens l. Subsp. rapaceum)
Kutlu, Naciye
In this study, celery roots were dried by the osmotic dehydration-assisted microwave drying method, and both osmotic dehydration (OD) and microwave conditions were optimized using response surface methodology (RSM). Utilizing a Box–Behnken experimental design, the drying time was minimized while maximizing the total phenolic compounds (TPC) in dried samples at different OD salt concentrations (10, 20 and 30 %), OD temperatures (30, 45 and 60 °C), OD durations (1, 2 and 3 h) and microwave powers (210, 350 and 490 W). The optimal drying conditions were identified as 15.05 % for OD salt concentration, 42.5 °C for OD temperature, 1.6 h for OD duration and 489.7 W for microwave power. Under these optimal conditions, the predicted values for time and TPC were 10 min and 104.38 mg GAE/100 g, respectively. The composite desirability value was 0.99 as a result of optimization. The experimental values for time and TPC were measured as 11 min and 97.45 mg GAE/100 g, respectively. The predicted and experimental values were found to be statistically similar to each other. A comparison with control groups designed to assess the effects of OD and microwave processes revealed that these processes significantly influenced both quality (TPC, antioxidant activity, color) and drying characteristics (drying time, rehydration rate). The greenness assessment value was calculated as 78 using the MoGAPI tool. Consequently, the application of OD pretreatment and use of the innovative microwave drying method as a final drying step in vegetable drying was successfully implemented to enhance the properties of the final product.
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İncele - MakaleAçık Erişim2024Scopus SJRQ2An optimization study for amino acid extraction from bee bread using choline chloride-acetic acid deep eutectic solvent and determination of individual phenolic profile
Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Bayram, Sinan; Ecem Bayram, Nesrin
In this study, the aim was to optimize the extraction conditions (molar ratio, time, temperature) in order to maximize the total individual amino acid (TAA) yield from bee bread, which is a fermented bee product, using choline chloride-acetic acid deep eutectic solvent (DES). In addition to the phenolic profile and amino acid profile investigated by LC-MS/MS, some spectrophotometric (total phenolic content, total flavonoid content, total proanthocyanidin content) and bioactivity (antioxidant/antimicrobial activity) tests were carried out on the extracts. Optimum conditions were determined as 1:2 for molar ratio, 15 min for time and 10 °C for temperature with optimization based on the TAA values of the different extracts (d: 0.99). Molar ratio and temperature were found to have statistically significant effects on TAA values. At optimum conditions, the amount of TAA obtained with DES (29.678 g/kg) was found to be approximately 35.2% and 54.4% higher than that obtained with ethanol and methanol extracts, respectively. All these results indicate that choline chloride-acetic acid DES can be chosen when preparing bee bread extracts, which are evaluated for different areas of use such as food and health due to active ingredient content. Higher active ingredients can be extracted by trying different DES combinations.
AtıfScopus7WoS9OpenAlex11İncele - MakaleAçık Erişim2022Scopus SJRQ1Optimization of Biocatalytic Production of Enantiopure (S)-1-(4-Methoxyphenyl) Ethanol with Lactobacillus senmaizuke Using the Box–Behnken Design-Based Model
Kavi, Mervenur; Özdemir, Akın; Dertli, Enes; Şahin, Engin
Enantiomerically pure (S)-1-(4-methoxyphenyl) ethanol is a significant molecule for the production of various drug intermediates. (S)-1-(4-methoxyphenyl) ethanol was synthesized from 4-methoxyacetophenone using Lactobacillus senmaizuke as a biocatalyst. In addition, optimization of experimental conditions is important to analyze the role of culture parameters for catalytic bioreduction reactions. For this particular purpose, the experimental conditions of pH, incubation period, temperature, and agitation speed were investigated with the Box–Behnken experimental design-based proposed optimization model. (S)-1-(4-methoxyphenyl) ethanol, which can be used for the synthesis of antihistamines, including diphenhydramine hydrochloride and loratadine cycloalkyl [b] indoles that have the treatment function for an allergic response, was obtained in > 99% conversion, > 99% enantiomeric excess and 96% yield with whole cells of L. senmaizukei at this optimization conditions: pH = 5.80, the temperature = 29 °C, incubation period = 50 h, and agitation speed = 155 rpm. The bioreduction of 4-methoxyacetophenone efficiency was importantly affected by the quadratic and linear effects of experimental design parameters. Besides, the results demonstrate the importance of design parameters for catalytic bioreduction reactions. It is also concluded that the results show the effectiveness of the Box–Behnken experimental design-based proposed model to obtain optimum operating conditions of design parameters for catalytic bioreduction reactions.
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