Sürdürülebilir Kalkınma Amaçları
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- MakaleAçık Erişim2024Scopus SJRQ2The Effect of Different Doses of Salt Stress on Germination and Emergence in Cannabis (Cannabis sativa L.) Seed Treated with Pre-Salicylic Acid
Gul, Volkan; Sefaoglu, Fırat; Cetinkaya, Hatice; Dinler, Burcu Seckin
It is known that salinity stress, one of the abiotic stress factors, significantly limits the germination, growth, development, and crop yield of plants. Therefore, this study was conducted to evaluate the effects of salinity (control, 50, 100 ve 150 mM) on seed germination and the effect of salicylic acid (control, 0.25, 0.50, 0.75 ve 1.00 mM) on seed germination to improve salt tolerance in cannabis seeds. The physiological characteristics of the plant, such as germination percentage, germination duration, germination index, radicle length, plumule length, wet radicle weight, dry radicle weight, wet plumule weight, and dry plumule weight, were analyzed. Depending on the salinity application dose, it was observed that there was a significant decrease in the germination parameters of cannabis seeds compared to the control. It was observed that salicylic acid treatments under salinity stress positively affected all the characters examined and reduced germination arrest due to increasing concentration levels. Although it is recommended to prepare seeds with 0.5 mM salicylic acid pre-application dose against salt stress of the cannabis plant,it is essential to expand the studies on its transfer to practice. As a result, salicylic acid will provide positive results that can be transferred to practice by increasing the resistance of cannabis plants against salinity, especially in agricultural soils with salt problems, as it will be more sensitive to pests and diseases.
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İncele - MakaleAçık Erişim2022Scopus SJRQ2Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product
Kara, Yakup; Can, Zehra; Kolaylı, Sevgi
In this work, a reverse phase high-performance liquid chromatographic method (RP-HPLC) with photo diode array (PDA) at 4 different wavelengths (250, 280, 320 and 360) for the determination of some polyphenols in some bee products (honey, pollen, and propolis) is developed. The analyses were carried out on C18 column (250 mm x 4.6 mm, 5 μm; GL Sciences), and the gradient program was used with a mobile phase A reservoir with 70% acetonitrile and the B reservoir with 2% acetic acid. The HPLC method was founded that limit of detection was in the range 0.022-0.0908 μg/mL; the limit of quantification was in the range 0.074-0.3027 μg/mL, all calibration curves were linear R2>0.995 within the range, the recovery range was 91.43-111.37% for 10 ppm and 98.44-101.68% for 40 ppm and relative error levels 0.0330-0.0290 respectively. The developed method was applied to some bee products available on the Turkey market. The study aimed at phenolic profiles of the bee products extracts were revealed by using 25 phenolic standards. The proposed method was optimized, quickly, and simple validated by evaluating the linear range, the limits of detection and quantification, the accuracy, the precious, the repeatability, and recoveries suitable for the phenolic analysis. It is concluded that evaluated and the quantitative determination of the bee products can be made quickly and reliably with the optimized method.
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