Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2023Scopus SJRQ2Determination of toxicity and radioprotective properties of bacterial and fungal eumelanin pigments
Bayram, Sinan; Aygün, Bünyamin; Karadayi, Mehmet; Alaylar, Burak; Güllüce, Medine; Karabulut, Abdulhalik
Purpose: Determination of the protective property of melanin, an organic polymer class consisting of phenolic and/or indolic compounds isolated from bacteria and fungi, against fast neutron radiation. To show that these melanin samples, which also have antioxidant and metal chelating properties, can be used as an active ingredient for a drug to be developed against neutrons used in nuclear research and medicine. Materials and methods: Bacterial and fungal media were prepared, and melanin pigments were produced and isolated. For molecular characterization of pigments, bacterial genomic DNA extraction, 16S rDNA gene amplification processes, and fungal genomic DNA extraction, ITS1, and ITS4 Gene Regions amplification were performed. The DEL assay was implemented to determine the genotoxicity properties of bacterial and fungal melanin pigments. Samples were prepared in a pad measuring 10 ml volume (60 × 15 mm) at a concentration of 0.2–1 microgram in 1% agarose gel for radiation-absorbed dose measurements. Absorption measurements were made using 241Am-Be fast neutron source and Canberra brand NP series BF3 gaseous detector to determine the neutron radiation absorption capacity of all samples. The results obtained to determine the absorption degrees of melanin samples were compared with paraffin and normal concrete, which are widely used in neutron radiation shielding studies. Results: Melanin pigments were obtained using different bacteria and fungi strains. Afterwards, the fast neutron radiation absorption capacity of these purified pigments were determined. Compared to reference samples, these pigments were found to have slightly lower radiation absorbing ability. In addition to these experiments, cytotoxicity tests were carried out using the Yeast DEL assay technique to evaluate the potential for use of these organic pigments in fields such as medicine and pharmacology. According to the results obtained from the tests, it was determined that these melanin samples did not have any toxic effects. Conclusion: It was determined that these melanin samples have the potential to be used as a radioprotective drug active substance to protect the tissues and cells of people exposed to neutron radiation after a nuclear accident or nuclear war. Giving a drug that will be developed by using these active ingredients before or after people are exposed to a radiation environment can provide great benefits.
AtıfScopus12WoS12OpenAlex17
İncele - MakaleAçık Erişim2021Scopus SJRQ3Determination of radioprotective and genotoxic properties of sulfamide derivatives
Aygün, Bünyamin; Alaylar, Burak; Aklncloǧlu, Akln; Allm, Bünyamin; Kocaman, Ebu Talip; Karadayl, Mehmet; Abu Al-Sayyed, Mohammed Ibrahim; Aksu, Seyma; Sakar, Erdem; Özpolat, Özgür Flrat
Some potential drug active substances with the ability to reduce the effects of radiation on human tissues and cells were investigated. For this purpose, eight different types of sulfamide derivatives were synthesized and nuclear radiation protection parameters were determined. Neutron radiation reduction parameters such as the half-value layer effective removal cross-sections, mean free path, and the number of particles passing through the sample were determined with GEANT4 code. Additionally, the gamma radiation attenuation parameters of the materials examined were determined using Phy-X/PSD software in the energy area of 0.015-15 MeV. These parameters are the half-value layer, mass attenuation coefficient, mean free path, exposure buildup factor and effective atomic number. Neutron radiation absorption experiments were applied using an 241Am-Be fast neutron source. All results obtained for neutron radiation were compared with paraffin and water. It has been found that the ability of sulfamide derivatives to absorb these radiations is superior to reference materials. To determine whether these derivatives could have adverse effects on human health, their genotoxic potential was determined using the Ames/Salmonella bacterial reversion test. The results showed that these derivatives can be considered genotoxically safe in tests at concentrations up to 5 mM. Thus, it is suggested that the derivative materials examined in this study can be used as active substances for a drug to be made for protection against both neutron and gamma radiation.
AtıfScopus10WoS9OpenAlex11İncele - MakaleAçık Erişim2021Scopus SJRQ3Development of SiO2 based doped with LiF, Cr2O3, CoO4 and B2O3 glasses for gamma and fast neutron shielding
Aygün, Bunyamin; Sakar, Erdem; Karabulut, Abdulhalik; Alim, Bunyamin; Sayyed, Mohammed, I; Singh, Vishwanath P.; Yorgun, Nergiz Yildiz; Özpolat, Ozgur Firat
In this study, the fast neutron and gamma-ray absorption capacities of the new glasses have been investigated, which are obtained by doping CoO,CdWO4,Bi2O3, Cr2O3, ZnO, LiF,B2O3 and PbO compounds to SiO2 based glasses. GEANT4 and FLUKA Monte Carlo simulation codes have been used in the planning of the samples. The glasses were produced using a well-known melt-quenching technique. The effective neutron removal cross-sections, mean free paths, half-value layer, and transmission numbers of the fabricated glasses have been calculated through both GEANT4 and FLUKA Monte Carlo simulation codes. Experimental neutron absorbed dose measurements have been carried out. It was found that GS4 glass has the best neutron protection capacity among the produced glasses. In addition to neutron shielding properties, the gamma-ray attenuation capacities, were calculated using newly developed Phy-X/PSD software. The gamma-ray shielding properties of GS1 and GS2 are found to be equivalent to Pb-based glass.
AtıfScopus19WoS19OpenAlex16İncele - MakaleAçık Erişim2020Scopus SJRQ2Investigation of neutron and gamma radiation protective characteristics of synthesized quinoline derivatives
Aygün, Bunyamin; Alaylar, Burak; Turhan, Kadir; Sakar, Erdem; Karadayi, Mehmet; Abu Al-Sayyed, Ibrahim; Pelit, Emel; Güllüce, Medine; Karabulut, Abdulhalik; Turgut, Zuhal
Purpose: Quinoline is formed by various natural compounds, such as alkaloids from the cinchona plant, which exhibit various biological activities, and is an important building material for the development of new drugs. Quinoline can be used in anti-radiation drug development but radiation interaction properties must be determined. Material and methods: In this study, six types of synthesized quinoline derivatives were used. Fast neutron removal cross-section, mean free path, half value layer and transmission number were theoretically determined by using GEometry ANd Tracking 4 and FLUktuierende KAskade simulation codes for neutron shielding. Neutron dose absorption rates were determined using the(241)Am-Be fast neutron source and the Canberra NP series portable BF(3)gas proportional neutron detector. Gamma radiation shielding parameters were determined by using WinXCom and PSY-X/PSD software. Additionally, the genotoxic potentials of the derivatives were assessed by using the Ames/Salmonellabacterial reversion assay. Results and conclusions: Neutron shielding parameters such as removal cross-section, mean free path, half value layer and transmission number were theoretically determined for fast neutrons. To determine neutron absorption capacity of quinoline derivatives, neutron absorption, experiments were conducted. In addition, gamma radiation shielding parameters were calculated such as the mean free path (MFP), mass attenuation coefficient (mu t), half value thickness layer (HVL) and effective atomic number (Zeff) in the energy range of 0.015-15 MeV. The results of the all quinoline derivatives have excellent fast neutron shielding power compared to ordinary concrete. In addition, all quinoline derivatives have been found to have the capacity to attenuate gamma radiation. Moreover, they absorb well in both types of radiation, do not cause secondary radiation, and they are genotoxically safe at the tested concentrations. This study has demonstrated that these products can be used as active ingredients for a drug to be developed against radiation.
AtıfScopus47WoS47OpenAlex52İncele - MakaleAçık Erişim2018Scopus SJRQ3Investigation of high-temperature-resistant rhenium–boron neutron shields by experimental studies and Monte Carlo simulations
Korkut, Turgay; Korkut, Hatun; Aygün, Bünyamin; Bayram, Özkan; Karabulut, Abdulhalik
In this study, novel rhenium–boron neutron-shielding high-temperature-resistant materials were designed. The considered samples, Re60–B40, Re58–B42, Re50–B50, and Re40–B60, with different concentrations of rhenium and boron were investigated to elucidate their neutron-shielding performances, and compare them with well-known neutron-shielding materials such as the 316LN quality nuclear steel. In addition to the experimental studies, Monte Carlo simulations were performed using the FLUKA and GEANT4 codes, where 4.5-MeV neutrons emitted by a 241Am–Be source were employed. Experimental equivalent dose rates, simulated track lengths, energy balances, and neutron mass absorption cross sections were discussed in detail.
AtıfScopus5WoS2OpenAlex4İncele