Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2025Scopus SJRQ1/r/bayburt/12789
  Characterization and in situ bioprotective efficacy of reuterin E81 produced by Limosilactobacillus reuteri E81 in white cheese model

  İspirli, Hümeyra; Öztürk, Hale İnci; Dertli, Enes

  This study focused on characterization and in situ application of reuterin E81, a potent antimicrobial compound produced by Limosilactobacillus (L.) reuteri E81, in white cheese. Strain E81 harbored the gene island containing pdu-cbi-cob-hem clusters responsible for reuterin production and this led to investigation of reuterin production for this strain. Initially, reuterin was synthesized from glycerol and characterized using NMR and FT-MIR analyses. Specific 1H and 13C NMR peaks confirmed the presence of reuterin components such as 3-hydroxypropionaldehyde and acrolein, while FT-MIR spectra indicated a distinct carbonyl (C=O) stretching band at 1717 cm−1, characteristic of aldehyde groups of reuterin system. Reuterin E81 showed dose dependent fungistatic and bactericidal effects, with complete inhibition of Gram-negative ones at lower doses. In white cheese production, four groups were prepared, including control and experimental groups with or without glycerol addition. The challenge test conducted over 30 days of cold storage demonstrated that white cheese samples containing L. reuteri E81 and glycerol achieved a significant reduction in microbial loads, with Escherichia (E.) coli counts decreasing from 6.4 log CFU/g to undetectable levels, which was attributed to in situ reuterin production. Meanwhile, total aerobic mesophilic bacterial counts declined progressively. These findings highlight the potential of in situ reuterin production as a natural strategy to enhance the microbiological safety of white cheese. However, it also inhibited the growth of S. thermophilus and L. reuteri cultures. The inhibitory effects of reuterin on beneficial lactic acid bacteria suggest the need for further optimization to balance food safety with starter culture viability.

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  AtıfScopus5·WoS5·OpenAlex6

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• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/11145
  Comparative quality assessment of plant-based kefir as a vegan alternative to traditional kefir

  Meral-Aktaș, Hacer; Bazu-Çırpıcı, Burcu; Aktaș, Haktan; Kadi̇roğlu, Hafsa; Çeti̇n, Bülent

  Plant-based milk is emerging as a promising alternative for vegan and vegetarian diets and for people who are allergic to animal products. In this study, kefir was produced using hazelnut, walnut, and cashew milk, and fermented with a combination of Bifidobacterium longum (human origin) and selected autochthonous microbial strains with probiotic potential found in traditional kefir. Significant variation was identified in the physicochemical, microbiological and sensory analyses of kefir produced with plant-based milk and cow milk kefir as a control. In particular, phenolic compound and total phenolic contents were found to be greater in plant-based kefir than in cow milk kefir. Notably, walnut kefir exhibited the highest total phenolic content at 843 mg GAE/L, compared to 83 mg GAE/L in cow milk kefir, highlighting a significant increase in antioxidant potential. On the other hand, the sensory properties of cow milk kefir were rated higher than those of kefir made with plant-based milk. These findings suggest that plant-based kefir can serve as a vegan beverage alternative, although further development is needed to improve flavour acceptability.

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  AtıfScopus5·WoS5·OpenAlex8

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• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/11035
  The prebiotic potential of alternansucrase-based glucooligosaccharides: the effects on short-chain fatty acids production and microbiome diversity

  Ozulku, Gorkem; Harris, Hannah C.; Savva, George M.; Ahn-Jarvis, Jennifer H.; İspirli, Hümeyra; Dertli, Enes; Warren, Frederick J.

  Glucooligosaccharides (GLOS) produced by glucansucrases via acceptor reactions are oligosaccharides that transit upper intestinal tract and evade digestion and may be considered to be a prebiotic. The prebiotic function of GLOS can be dictated by the type of acceptor sugar used during its production. Therefore, the prebiotic effect on the gut microbiome and short chain fatty acids (SCFAs) production can be used to inform the selection of acceptor sugars. In this study, alternansucrase-based GLOS derived from different acceptor sugars (melibiose, cellobiose and lactose) and inulin (positive control) were investigated using in vitro colon model fermentation to examine their prebiotic effect and SCFAs production. Microbiome composition was determined using shotgun metagenomics. SCFAs and lactate formation was determined using 1H NMR spectroscopy. Fusicatenibacter saccharivorans and Bacteroides uniformis appeared to increase in abundance after 24 h following fermentation of all three GLOS. The highest abundance for Bifidobacterium longum was seen after supplementation with lactose derived GLOS. Fermentation of GLOS appeared to produce around twice as much acetate than fermentation with inulin, while similar amounts of other SCFAs and lactate were produced. Melibiose derived GLOS supplementation in healthy 3 donors increased F. saccharivorans while inulin supplementation promoted Lactobacillales in order level. This study suggests that there may be beneficial effects of GLOS's on gut microbiome composition and metabolite composition, relative to the effects seen for inulin.

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  AtıfScopus1·OpenAlex1

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• MakaleAçık Erişim2023Scopus SJRQ1/r/bayburt/12960
  Characterization of the black cumin (Nigella sativa L.) honey from Türkiye

  Kemal, Mehmet; Esertaş, Ülkü Zeynep Üreyen; Kanbur, Esra Demir; Kara, Yakup; Özçelik, Ali Erdem; Can, Zehra; Kolaylı, Sevgi

  This case report study investigated the botanical, physicochemical, and phenolic compositions, as well as the antioxidant and antimicrobial properties of varying amounts of black cumin honey (Nigella sativa) from Burdur region of Türkiye. The samples had an average moisture value of 17.23% and a Nigella sativa pollen ratio ranging from 19% to 51%. Electrical conductivity (EC) values ranged from 0.36 to 0.54 mS/cm. The average color values of Hunter (L, a, b) were 43.55 (L*), 32.77 (a*), and 64.52 (b*), respectively. The proline contents were ranged from 875 to 1624 mg/kg. The average F/G ratio and F+G value were 1.43±0.11 and 68.75±4.75, respectively. The average of total phenolic (TP) and total flavonoid (TF) contents were 36.87±7.00 mg GAE/100 g and 5.63±1.45 mg QUE/100 g, respectively. The antioxidant capacities of the samples were tested using the ferric reducing/antioxidant power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays, with mean values of 179.40±25.35 μmol FeSO4.7H2O/100 g and 46.07±12.87 mg/mL, respectively. The phenolic composition of the samples were tested using the HPLC-PDA method with 25 phenolic standards. Gallic acid, p-OH benzoic acid, t-cinnamic acid, p-coumaric acid, chrysin, and pinocembrin were determined as common components. The samples exhibited high antimicrobial activities against Staphylococcus aureus, Escherichia coli, Chromobacterium violaceum, Bacillus cereus, Klebsiella pneumoniae, and Acinetobacter haemolyticus, but did not affect Candida albicans, Candida parapilopsis, or Mycobacterium smegmatis.

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  AtıfScopus27·WoS27·OpenAlex33

  İncele
• MakaleYalnız künye2022Scopus SJRQ1/r/bayburt/13215
  Characterisation and functional roles of a highly branched dextran produced by a bee pollen isolate Leuconostoc mesenteroides BI-20

  Yilmaz, Mustafa Tahsin; İspirli, Hümeyra; Taylan, Osman; Taşdemir, Volkan; Sagdic, Osman; Dertli, Enes

  Lactic Acid Bacteria (LAB) from different niches can be responsible for the production of distinct exopolysaccharides (EPS) that might possess important structural and technological features. In this respect, the aim of this study was to isolate an EPS producer LAB strain from bee pollen environment. Leuconostoc mesenteroides BI-20 with a slimy-mucoid colony morphology was identified from bee pollen and the structural, technological and functional characteristics of EPS produced by this strain were determined. EPS BI-20 was a highly branched dextran containing 20% (1 → 3)-linked α-D-glucose branches determined by 1H and 13C NMR analysis. The presence of (1 → 6)/(1 → 3)-linked α-D-glucose linkages in dextran BI-20 was also confirmed by FTIR analysis. Dextran BI-20, with a molecular weight of 1 × 108 Da, possessed strong thermal properties, amorphous nature and highly branched and fibrous microstructural characteristics determined by DSC, TGA, XRD and SEM analysis, respectively. In terms of functional roles, dextran BI-20 demonstrated strong antioxidant capacity detected by ABTS and CUPRAC tests. Finally, no digestion was observed in dextran BI-20 under simulated gastric conditions. Results of this study unveiled techno-functional characteristics of dextran BI-20 produced by a bee pollen isolate LAB strain.

  AtıfScopus39·WoS36·OpenAlex39

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• MakaleYalnız künye2022Scopus SJRQ1/r/bayburt/12005
  Comparison of the probiotic characteristics of Lactic Acid Bacteria (LAB) isolated from sourdough and infant feces

  Kaya, Yasemin; Erten, Tayyibe; Vurmaz, Melike; İspirli, Hümeyra; Şimşek, Ömer; Dertli, Enes

  Probiotics are beneficial microorganisms with certain characteristics such as survival under gastrointestinal conditions, adhesion to intestinal surface and different functions which result in several health benefits to host. With this regards, this study aimed to isolate Lactic Acid Bacteria (LAB) from sourdough and human feces as two different environments and to test their potential probiotic functions. In total nine distinct isolates were assessed with several probiotic functions such as in vitro adhesion to human colon cells, antimicrobial activities and survival under harsh gastrointestinal conditions. In general, distinct strains showed strong antimicrobial activity against the pathogenic and fungal strains tested in this study. Importantly, the adhesion levels to HT29 cells were determined between 0.29 % and 9.54% and Lactobacillus paracasei F7B showed the highest adhesion. All isolates tolerated the bile salt after 24 h and the infant feces isolates showed good survival characteristics at pH 4.

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  AtıfScopus18·WoS15·OpenAlex18

  İncele
• MakaleYalnız künye2022Scopus SJRQ1/r/bayburt/11864
  Bioactive compounds of deep eutectic solvents extracts of Hypericum perforatum L.: Polyphenolic- organic acid profile by LC-MS/MS and pharmaceutical activity

  Bayram, Sinan; Kutlu, Naciye; Gerçek, Yusuf Can; Çelik, Saffet; Ecem Bayram, Nesrin

  Deep eutectic solvents (DESs) are known as environmentally-friendly solvents that have emerged as an alternative to conventional organic solvents in recent years. In this study, the effects of ten different DESs on the extraction of bioactive components from the Hypericum perforatum were investigated. Total phenolic content, total flavonoid content, phenolic profile (32 phenolics), organic acid profile (52 organic acids), as well as antioxidant and antimicrobial activities of H. perforatum extracts prepared using DESs and ultrasonic-assisted extraction method were determined. Total phenolic, total flavonoid content, and antioxidant activities of extracts were determined between 3.10 ± 0.86–16.64 ± 2.09 mg GA/g, 0.02 ± 0.00–12.29 ± 0.30 mg QE/g, and 8.40 ± 0.50–44.78 ± 2.66 mg TE/g, respectively. DES2 (choline chloride:ethylene glycol) and DES7 (choline chloride:lactic acid) demonstrated higher extraction success than other DESs for extracting phenolics and organic acids, respectively. DESs prepared using organic acid as hydrogen bond donor were found to have an antibacterial effect against all bacterial samples. However, the inhibitory effect against yeast-like fungi was observed in a narrower spectrum. Moreover, the results from principal component analysis also confirmed that the type of solvent used for extraction has a significant influence on the phenolics, organic acid, and other parameters. As a result, it was seen that DES components directly affect the extraction efficiency of target compounds from H. perforatum. This is a first and original report about use of DESs in extracting different bioactive components from H. perforatum.

  AtıfScopus33·WoS31·OpenAlex32

  İncele
• MakaleAçık Erişim2022Scopus SJRQ1/r/bayburt/11652
  Identification of Lactic Acid Bacteria (LAB) from pre-fermented liquids of selected cereals and legumes and characterization of their exopolysaccharides (EPS)

  Yalmanci, Dilara; İspirli, Hümeyra; Dertli, Enes

  Recently, isolation of Lactic Acid Bacteria (LAB) strains with important technological functions from plant-based products has gained special interests related with the increment of consumer preferences to the plant-based fermented products. In this context, various cereals and legumes that were underwent to germination or not were selected for the isolation of exopolysaccharide (EPS) producer LAB strains in this study. Following genotypic discrimination, 17 distinct strains belonging to Weissella confusa, Weissella cibaria, Lactococcus lactis subsp. hordniae, Leuconostoc lactis, Lactiplantibacillus plantarum and Leuconostoc mesenteroides were identified from pre-ferment liquids. Two strains Weissella confusa W-16 and Leuconostoc lactis GW-6 were the highest EPS producers, and their EPSs were further characterized. The 1H and 13C NMR results revealed that both strains were dextran producers containing (1 → 3)-linked α-D-glucose units as branches with 9.2% and 6.9% levels for Weissella confusa W-16 and Leuconostoc lactis GW-6, respectively. FTIR analysis also supported the dextran structure and demonstrated the functional groups within the dextrans. A high level of thermal stability was observed in both dextrans detected by TGA and DSC analysis. Both dextrans were in amorphous characteristics determined by XRD analysis. These findings demonstrate that pre-fermented liquids of different cereals and legumes can be source of technologically important LAB strains with EPS production characteristics.

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  AtıfScopus41·WoS41·OpenAlex42

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• MakaleAçık Erişim2021Scopus SJRQ1/r/bayburt/11953
  Synthesis and characterization of cellobiose-derived oligosaccharides with Bifidogenic activity by glucansucrase E81

  İspirli, Hümeyra; Bowman, Michael J.; Skory, Christopher D.; Dertli, Enes

  Cellobiose-derived oligosaccharides were produced from acceptor reactions with glucansucrase E81 and their prebiotic potentials were determined in this study. The major product was a mixture of trisaccharides formed by the addition of an α-linked glucose to cellobiose at either the 2-O position of the reducing end (86%) glucose or the 6-O position of the non-reducing end glucose (11%) demonstrated by NMR spectroscopy and LC-MS-LTQ analysis. The purified DP 3 cellobiose-derived oligosaccharides were then tested for in vitro human gastric juice and α-amylase digestion and no digestion was observed during the 6 h digestion period. Importantly, DP 3 cellobiose-derived oligosaccharides promoted the growth of Bifidobacteria at high levels demonstrating a Bifidogenic effect and a mild level of proliferation was observed for Lactobacilli suggesting the prebiotic roles of cellobiose-derived oligosaccharides.

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  AtıfScopus7·WoS5·OpenAlex6

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