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- MakaleYalnız künye2024Scopus SJRQ2The wound healing effect and DNA damage repair of exopolysaccharide extracted from Weissella cibaria MED17
Kahraman-Ilıkkan, Özge; Akad Dinçer, Selin; Bağdat, Elif Şeyma; Çağlar, Işılsu; Dertli, Enes; İspirli, Hümeyra; Şahin, Feride İffet
Lactic acid bacteria can synthesize exopolysaccharides (EPSs), and some of these EPSs have pharmaceutical or medicinal properties. However, the wound healing capacities of EPSs have rarely been reported. In this study, EPS extracted from Weissella cibaria MED17 was used to investigate wound healing capacity. MED17 EPS was previously characterized and antioxidant capacity was also demonstrated. The application dose of the compound was determined by the MTT assay. The comet assay was applied to reveal the free radical scavenging activity and protection capacity of MED 17 against DNA damage by inducing oxidative stress and DNA damage with Mitomycin-C (MMC) in the cells. The comet assay showed that 15 mg/ml MED 17 EPS could ameliorate DNA damage by 50%. Moreover, the scratch test investigated the wound-healing effect of MED 17 EPS on HT-29 colon cells. MED 17 EPS could stimulate the migration of HT-29 and ameliorate MMC-induced DNA damage. However, further studies need to be conducted to clarify the mechanisms of the wound-healing process of MED 17.
AtıfScopus5WoS5OpenAlex5
İncele - MakaleYalnız künye2022Scopus SJRQ1Bioactive and technological properties of an α-D-glucan synthesized by Weissella cibaria PDER21
Yilmaz, Mustafa Tahsin; Ispirli, Humeyra; Taylan, Osman; Alamoudi, Mohammed; Dertli, Enes
A slimy-mucinous-type colony of EPS-producing Weissella cibaria PDER21 was isolated and identified. The monomer composition was glucose, showing that the EPS is a glucan type homopolysaccharide, The core structure of (1 → 6)-linked α-D-glucose units including (1 → 3)-linked α-D-glucose branches at a ratio of 93.4/6.6 was revealed by 1H and 13C NMR spectra and confirmed by FTIR analysis. The glucan showed a superior thermal stability with almost no degradation in structure up to 300 °C. XRD analysis revealed the amorphous structure while SEM analysis confirmed the layer-like morphology. The glucan had an antioxidant activity (89.5%), water-holding capacity (103.7%) and water solubility index (80.7%) values, suggesting that the glucan had a strong level of antioxidant properties; good water binding capacity and excellent solubility. The glucan PDER21 is a polysaccharide possessing a good combination of technical and functional attributes, suggesting a great deal of potential for use in the food industry.
AtıfScopus42WoS41OpenAlex40
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