Sürdürülebilir Kalkınma Amaçları

• 1

Yazarlar

• Dertli, Enes(2)
• Arici, M.(1)
• Bekar, Fevzi(1)
• Dertli, E.(1)
• Dikmetas, Dilara Nur(1)
• Ermis, Ertan(1)
• Erten, Hüseyin(1)
• Fevzi Bekar(1)
• Kamiloglu, Aybike(1)
• Karbancıoglu-Guler, Funda(1)
• Oztekin, Sebahat(1)
• Poyraz, Ecem(1)
• Sagdic, O.(1)
• Tatlisu, N.B.(1)
• Toker, O.S.(1)
• Yilmaz, M.T.(1)
• Yilmaz, Mustafa Tahsin(1)
• Zehir Şentürk, Duygu(1)
• Şimşek, Ömer(1)

• MakaleYalnız künye2025Scopus SJRQ2/r/bayburt/11806
  A novel exopolysaccharide from cold-adapted yeast Rhodotorula glutinis, along with structural, rheological, antioxidant, and antibiofilm properties

  Oztekin, Sebahat; Dikmetas, Dilara Nur; Karbancıoglu-Guler, Funda

  In the present study, a novel exopolysaccharide EPS-BMD26 produced by cold-adapted yeast Rhodotorula glutinis was structurally examined, along with its rheological, antioxidant, and antibiofilm properties. High-performance liquid chromatography (HPLC) analysis revealed glucose and galactose as two monosaccharide constituents. The functional groups within the EPS-BMD26 structure were detected by Fourier transform infrared (FT-IR) spectroscopy. The 1H and 13C nuclear magnetic resonance (NMR) spectra of EPS-BMD26 also confirmed its β-D-glucan type with galactose residues with an average molecular weight of 118 kDa. Differential scanning calorimetry (DSC) analysis revealed that EPS-BMD26 had a high level of thermal stability, up to 326.16 °C. Scanning electron microscopy (SEM) analysis demonstrated that EPS-BMD26 had a porous microstructure with fissures. X-ray diffraction (XRD) analysis revealed its semi-crystalline nature. EPS-BMD26 showed moderate and concentration-dependent antioxidant potential recruiting different methods, including •OH, DPPH•, CUPRAC, and ABTS radical scavenging activities. Water-soluble EPS-BMD26 exhibited high water holding capacity of 190 ± 0.22% with a water solubility index of 60.6 ± 8.3%. At 1.25 mg mL-1, EPS-BMD26 inhibited biofilm formation of Staphylococcus aureus ATCC 25923 by 79.5%. Rheological characterization revealed that the aqueous solution of EPS-BMD26 had shear thinning and pseudoplastic behavior. Given these properties, EPS-BMD26 may be a promising bio-hydrocolloid, antibiofilm agent, and food additive for industrial applications.

  AtıfScopus15·WoS15·OpenAlex17

  İncele
• MakaleYalnız künye2024Scopus SJRQ2/r/bayburt/13055
  Effect of different carbon sources on exopolysaccharide production of Lactiplantibacillus pentosus KS27 and optimization of production conditions using Box-Behnken designing

  Bekar, Fevzi; Kamiloglu, Aybike

  This study aimed to determine the exopolysaccharide (EPS) production of Lactiplantibacillus pentosus KS27 depending on temperature, incubation time, and medium composition and to determine the optimum conditions. Characterization was carried out for the exopolysaccharide produced under optimum conditions. In the study, three different conditions (25 °C, 35 °C, 45 °C) were used for temperature, glycerol, and sucrose as media, and three different periods (24 h, 36 h, and 48 h) were used. It was determined that the solubility of EPS produced under optimal conditions had the highest water content (98 ± 0.01%), while the water binding capacity was also found to be as high as 1970 ± 0.01% and complied with common EPS characteristics. Differential scanning calorimeter analysis showed a gradual deterioration up to 191.88 °C and a slow decline up to 400 °C. FTIR analysis deciphered peak values between 3289 and 517 cm−1, and functional groups were detected. When the nuclear magnetic resonance (NMR) spectrum was examined, a heterogeneous structure appropriate to the structure of EPS was found by defining the peak entities in three separate regions.

  AtıfScopus5·WoS5·OpenAlex6

  İncele
• Yüksek Lisans TeziAçık Erişim2022/r/bayburt/17205
  Lactobacıllus pentosus KS27'nin ekzopolisakkarit üretimi ve üretim koşullarının optimizasyonu

  Fevzi Bekar

  Bu çalışma ile Lactobacillus pentosus KS27'nin, ortam sıcaklığı, inkübasyon süresi ve besiyeri bileşimine bağlı olarak ekzopolisakkarit (EPS) üretimi ve bu şartların optimum koşullarının belirlenmesi amaçlanmıştır. Optimum şartlara bağlı olarak üretilen ekzopolisakkaritin karakterizasyonu gerçekleştirilmiştir. Yapılan çalışmada ortam sıcaklığı için 3 ayrı koşul (25oC-35oC-45oC) belirlenmiş, besiyeri olarak gliserol ve sükroz kullanılmış, gelişim süresi olarak ise yine 3 farklı süre (24-36-48 saat) belirlenmiştir. Belirlenen değişkenlere göre Box-Behnken deney matriksi ile 27 farklı EPS üretim denemesi belirlenmiştir. Bu denemelerde en yüksek verim 25oC'de 36 saatlik inkübasyon sonucunda alınmıştır. Karbon kaynağı olarak %7,5 sükroz konsantrasyonu ve %7,5 gliserol konsatrasyonu kullanılmış ve 1913,75 mg/L EPS elde edilmiştir. Denemelerde en düşük verim ise 25oC, 36 saat inkübasyon süresine sahip olan ve %7,5 sükroz konsantrasyonu %0 gliserol konsantrasyonu bulunan deneme olmuştur. Bu denemede elde edilen EPS miktarı 213,75 mg/L olmuştur. Elde edilen veriler doğrultusunda optimum nokta belirlenmeye çalışılmış ve istenirlilik derecesi 1 olan 100 koşuldan en yüksek verim elde etme amacıyla sükroz konsantrasyonunun %5,31, gliserol konsantrasyonunun %10,92 sıcaklığın 26oC ve inkübasyon süresinin 46,14 saat olduğu koşul ile üretim yapıldığında 2158 mg/L EPS üretimi gerçekleşeceği verisine ulaşılmıştır. Belirtilen koşullarda üretim gerçekleştirildiğinde 2178 mg/L EPS üretimi sağlanmıştır. Optimum koşullarda üretilen EPS'nin çözünürlüğünün en yüksek su ile (%98 ± 0,01) sağlandığı belirlenirken, su bağlama kapasitesi de %1970 ± 0,01 gibi yüksek bir değer bulunmuş ve ortak EPS özelliklerine uyum göstermiştir. Diferansiyel Tarama Kalorimetre analizinde 250oC civarına kadar kademeli bozulma ve 400oC'ye kadar yavaş bir bozulma görülmüştür. FTIR analizi ile 3289 ve 517 cm-1 arasında pik değerleri alınmış ve fonksiyonel gruplar belirlenmiştir. NMR spektrumu incelendiğinde, pik varlıkları üç ayrı bölge ile tanımlanarak EPS'lerin yapısına uygun heterojen yapı çözümlenmiştir.

  İncele
• MakaleAçık Erişim2020Scopus SJRQ2/r/bayburt/13235
  Structural and technological characterization of ropy exopolysaccharides produced by Lactobacillus plantarum strains isolated from Tarhana

  Zehir Şentürk, Duygu; Dertli, Enes; Erten, Hüseyin; Şimşek, Ömer

  Exopolysaccharide producing starter cultures enable manufacturing “clean labeled” foods with improved textural and nutritional properties. The structural and technological analyses were performed on the ropy exopolysaccharides of six Lactobacillus plantarum. The incubation temperature, time and pH affected the exopolysaccharide production and high exopolysaccharide was produced in the presence of sucrose and maltose. The viscosity of exopolysaccharide was high at acidic conditions except PFC311E that showed viscous at neutral pH. Lactobacillus plantarum strains produced between 120 and 400 mg/L exopolysaccharide in which the highest was observed at L. plantarum PFC311. Exoploysaccharides were degraded over 300 °C except PFC311E that degraded at 295.7 °C. The NMR analyses revealed that the exopolysaccharides were synthesized by α1-6, α1-3 and α1-4 bonds with glucose, galactose and fructose moieties. In conclusion, L. plantarum PFC311 produced ropy exopolysaccharide with different structural, rheological and thermal properties and reveals potential to be used in food industry.

  AtıfScopus42·WoS37·OpenAlex36

  İncele
• MakaleAçık Erişim2020/r/bayburt/12129
  Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

  Ermis, Ertan; Poyraz, Ecem; Dertli, Enes; Yilmaz, Mustafa Tahsin

  Response surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables, temperature (30, 36 and 42 °C), incubation time (18, 33 and 48 h) and initial pH of growth medium (4.5, 5.5, and 6.5) were studied. Under optimum growth conditions, the amount of exopolysaccharide derived from Lactobacillus brevis E25 ranged from 10 to 35 gL-1. The size of EPS molecules ranged from 105 to 107 Da. Infra red spectrum analysis showed characteristics absorption peaks indicating the presence of-OH, C=O and C-H groups. Furthermore, only glucose was detected as monosaccharide in exopolysaccharide structure, revealing that the structure of exopolysaccharide is a homopolymeric glucan type. Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.

  AtıfScopus18·OpenAlex18

  İncele
• MakaleAçık Erişim2015Scopus SJRQ1/r/bayburt/13903
  Effect of in situ exopolysaccharide production on physicochemical, rheological, sensory, and microstructural properties of the yogurt drink ayran: An optimization study based on fermentation kinetics

  Yilmaz, M.T.; Dertli, E.; Toker, O.S.; Tatlisu, N.B.; Sagdic, O.; Arici, M.

  Exopolysaccharide (EPS)-producing starter cultures are preferred for the manufacture of fermented milk products to improve rheological and technological properties. However, no clear correlation exists between EPS production and the rheological and technological properties of fermented milk products such as the yogurt drink ayran. In this study, 4 different strain conditions (EPS- and EPS+ Streptococcus thermophilus strains) were tested as a function of incubation temperature (32, 37, or 42°C) and time (2, 3, or 4 h) to determine the effect of culture type and in situ EPS production on physicochemical, rheological, sensory, and microstructural properties of ayran. Furthermore, we assessed the effect of fermentation conditions on amounts of EPS production by different EPS-producing strains during ayran production. A multifactorial design of response surface methodology was used to model linear, interaction, and quadratic effects of these variables on steady shear rheological properties of ayran samples and in situ EPS production levels. The physicochemical and microbiological characteristics of ayran samples altered depending on incubation conditions and strain selection. Steady shear tests showed that ayran samples inoculated with EPS+ strains exhibited pseudoplastic flow behavior. Production of ayran with EPS- strain (control sample) resulted in the lowest apparent viscosity values (η50), whereas those produced with the combination of 2 EPS+ strains yielded ayran with notably increased η50 values. We concluded that incubation time was the variable with the greatest effect on η50, consistency coefficient (K), and flow behavior index (n) values. In situ EPS production was also affected by these conditions during ayran fermentation in which strain-specific metabolism conditions were found to be the most important factor for EPS production. In addition, these findings correlated the amount of in situ EPS produced with the rheological properties of ayran. Scanning electron microscopy images of the samples showed differences in structural features, revealing a prominent network strand structure in the ayran samples inoculated with the admixture of 2 EPS-producing strains incubated at 37°C for 3 h. These results provide useful information for large-scale production of ayran by the dairy industry.

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  AtıfScopus79·WoS65·OpenAlex92

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