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Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro study

MakaleAçık ErişimScopus SJRQ1
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Özet

Aldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2′5′ ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, α-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Ki values and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR.

Anahtar Kelimeler

Aldose reductasediabetic complicationsinhibitionphenolic acids
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ScopusScopusWeb of ScienceWeb of SciencePubMedPubMed
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Atıflar
Scopus
61
WoS
60
OpenAlex
64
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorAlim, Zuhal
dc.contributor.authorKilinç, Namik
dc.contributor.authorŞengül, Bülent
dc.contributor.authorBeydemir, Şükrü
dc.date.accessioned2026-10-29T19:11:30.185033337Z
dc.date.available2026-10-29T19:11:30.185033337Z
dc.date.issued2017
dc.description.abstractAldose reductase (AR) inhibitors have vital importance in the treatment and prevention of diabetic complications. In this study, rat kidney AR was purified 19.34-fold with a yield of 3.49% and a specific activity of 0.88 U/mg using DE-52 Cellulose anion exchange chromatography, gel filtration chromatography and 2′5′ ADP Sepharose-4B affinity chromatography, respectively. After purification, the in vitro inhibition effects of some phenolic acids (tannic acid, chlorogenic acid, sinapic acid, protocatechuic acid, 4-hydroxybenzoic acid, p-coumaric acid, ferulic acid, vanillic acid, syringic acid, α-resorcylic acid, 3-hydroxybenzoic acid and gallic acid) were investigated on purified enzyme. We determined IC50, Ki values and inhibition types of these phenolic acids. As a result, tannic and chlorogenic acid had a strong inhibition effect. On the other hand, gallic acid had a weak inhibition effect. In this study, all phenolic acids except for chlorogenic acid and p-coumaric acid showed non-competitive inhibition effects on rat kidney AR.
dc.identifier.doi10.1080/14756366.2016.1250752
dc.identifier.eissn1475-6374
dc.identifier.endpage284
dc.identifier.issn1475-6366
dc.identifier.issue1
dc.identifier.pmcidPMC6009866
dc.identifier.pmid28111996
dc.identifier.scopus2-s2.0-85013846384
dc.identifier.startpage277
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/bayburt/13817
dc.identifier.volume32
dc.identifier.wosWOS:000392591100021
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAldose reductase
dc.subjectdiabetic complications
dc.subjectinhibition
dc.subjectphenolic acids
dc.subject.classificationBiochemistry & Molecular Biology
dc.subject.classificationChemistry, Medicinal
dc.subject.classificationPharmacology & Pharmacy
dc.subject.otherAldehyde Reductase
dc.subject.otherAnimals
dc.subject.otherElectrophoresis, Polyacrylamide Gel
dc.subject.otherHydroxybenzoates
dc.subject.otherIn Vitro Techniques
dc.subject.otherKidney
dc.subject.otherRats
dc.subject.other3 hydroxybenzoic acid
dc.subject.other4 hydroxybenzoic acid
dc.subject.otheradenosine diphosphate
dc.subject.otheralpha resorcylic acid
dc.subject.othercellulose
dc.subject.otherchlorogenic acid
dc.subject.otherde 52 cellulose
dc.subject.otherferulic acid
dc.subject.othergallic acid
dc.subject.otherpara coumaric acid
dc.subject.otherphenol derivative
dc.subject.otherprotocatechuic acid
dc.subject.othersepharose
dc.subject.othersinapic acid
dc.subject.othersyringic acid
dc.subject.othertannin
dc.subject.otherunclassified drug
dc.subject.othervanillic acid
dc.subject.otherhydroxybenzoic acid derivative
dc.subject.otherphenolic acid
dc.subject.otheraffinity chromatography
dc.subject.otheranion exchange chromatography
dc.subject.otherantioxidant activity
dc.subject.otherArticle
dc.subject.otherdrug inhibition
dc.subject.otherenzyme activity
dc.subject.otherenzyme purification
dc.subject.othergel filtration chromatography
dc.subject.otherIC50
dc.subject.otherin vitro study
dc.subject.othernonhuman
dc.subject.otherpriority journal
dc.subject.otherprotein folding
dc.subject.otherrat
dc.subject.otheranimal
dc.subject.otherantagonists and inhibitors
dc.subject.otherenzymology
dc.subject.otherpolyacrylamide gel electrophoresis
dc.titleInhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an in vitro study
dc.typeArticle
rdl.citation.openalex64
rdl.citation.scopus61
rdl.citation.wos60
rdl.departmenta Department of Chemistry, Faculty of Science and Arts , Ahi Evran University , Kirşehir , Turkey
rdl.departmentb Department of Medical Services and Techniques, Vocational School of Health Service , Iğdir University , Iğdir , Turkey
rdl.departmentc Deparment of Health Care Service, Vocational School of Health Service , Bayburt University , Bayburt , Turkey
rdl.departmentd Department of Chemistry, Faculty of Sciences , Atatürk University , Erzurum , Turkey
rdl.departmente Department of Biochemistry, Faculty of Pharmacy , Anadolu University , Eskişehir , Turkey
rdl.oa.statusgold
rdl.oa.statusgreen
rdl.oa.urlhttps://www.tandfonline.com/doi/pdf/10.1080/14756366.2016.1250752?needAccess=true
rdl.scopus.quartileQ1
rdl.sdgSDG03
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)