Sürdürülebilir Kalkınma Amaçları

• 3

• MakaleAçık Erişim2021/r/bayburt/14671
  L929 Hücre Hattında Lipopolisakkaritle İndüklenen Sitotoksisite Modeli Üzerine Ferulago pauciradiata Boiss. & Heldr. Bitkisinin Kökünden İzole Edilen Prantşimgin Bileşiğinin Etkisinin İncelenmesi

  Karabulut, Sevgi; Karakaya, Songül; Kutlu, Zerrin; Postallı, Fatma Gül Delimustafaoğlu

  L929 hücre hattında oluşturulan lipopolisakkarit (LPS) kaynaklı sitotoksisite modelinde Ferulago pauciradiata (Apiaceae) bitkisinin kök kısmından izole edilen prantşimgin (Prn) bileşiğinin etkisinin incelenmesi. L929 hücre hatları, 37°C'de %5 CO2'li inkübatörde standart hücre kültürü prosedürleri kullanılarak son konsantrasyonu 2 µL olacak şekilde Prn saf maddesi ve 1 saat sonra son konsantrasyonu 1 µL olacak şekilde LPS uygulandı. LPS uygulamasının ardından, gerekli olan 24, 48 ve 72 saat inkübasyon süreleri sonunda sitotoksik etkilerin belirlenmesi amacıyla kolorimetrik bir metot olan MTT protokolü uygulandı. IC50 değerleri, Prn için 0.28 µg/mL ve LPS için ise 1 µg/mL olarak hesaplandı. L929 hücre hatlarına LPS uygulanmasının zamana bağlı olarak hücresel indekste anlamlı derecede azalmaya neden olmuştur. Ayrıca Prn+LPS gruplarında ise sadece LPS uygulanan grubuna göre azalmış hücre indeksini, anlamlı derecede artırdığı hatta kontrole yaklaştırdığı tespit edilmiştir.L929 hücre hattına uygulana LPS ile oluşturulan sitotoksisite ve hücre hasarını Pnr uygulaması sonucunda düzeldiği tespit edildi.

  • 
  AtıfOpenAlex2

  İncele
• MakaleAçık Erişim2020/r/bayburt/14959
  Correlation of IGF-I and IGFBP5 Gene Expression in Schizophrenia Patients

  Ozcan, Halil; Karabulut, Sevgi; Yeloğlu, Çağdaş Hünkar

  Objective: Schizophrenia is a severe mental disorder affecting more than 21 million people worldwide. Disruptions of the GH-IGF-I axis likely lead to schizophrenia by causing deficits in early stages of neurodevelopment and low IGF-I levels are related to pre/postnatal growth and schizophrenia. IGF axis has effective role on healthy cell growth both prenatal and postnatal. IGF-I function effecting cancer cells is regulated by IGFBP-5. Several studies showed that IGF-I expression is low in schizophrenia patients. Aim of this study is to investigate relation of IGF-I and IGFBP-5 mRNA expression levels in Schizophrenia. Materials and Methods: Fırstly, we collected the serum samples from patients who are diagnosed with schizophrenia and healthy control group. Then RNA was isolated from these serum samples. RNA expression was investigated by real time PCR. Results were evaluated using delta delta Ct. In this study IGF-I and IGFBP5 gene expression levels were calculated. For this purpose a portion of serum taken for routine tests was used. Results: Results of this study showed that there is no significant difference of IGF-I and IGFBP5 gene expression levels between patients and healthy subjects. On the other hand an important negative correlation between IGFBP5 expression level and age was found (p<0,05). Beside that there was not any association between PANNS points and gene expression levels. Conclusion: Consequently, relation of PANSS and gene expressions was not significant. Otherwise IGFBP5 and IGF-I mRNA expression levels had not an important relation for schizophrenia patients.

  • 
  İncele
• MakaleAçık Erişim2017Scopus SJRQ2/r/bayburt/13619
  Comparison of telomere length and insulin-like growth factor-binding protein 7 promoter methylation between breast cancer tissues and adjacent normal tissues in Turkish women

  Kaya, Zehra; Akkiprik, Mustafa; Karabulut, Sevgi; Peker, Irem; Gullu Amuran, Gokce; Ozmen, Tolga; Gulluoglu, Bahadır M.; Kaya, Handan; Ozer, Ayse

  Background: Both insulin-like growth factor-binding protein 7 (IGFBP7) and telomere length (TL) are associated with proliferation and senescence of human breast cancer. This study assessed the clinical significance of both TL and IGFBP7 methylation status in breast cancer tissues compared with adjacent normal tissues. We also investigated whether IGFBP7 methylation status could be affecting TL. Methods: Telomere length was measured by quantitative PCR to compare tumors with their adjacent normal tissues. The IGFBP7 promoter methylation status was evaluated by methylation-specific PCR and its expression levels were determined by western blotting. Results: Telomeres were shorter in tumor tissues compared to controls (P<.0001). The mean TL was higher in breast cancer with invasive ductal carcinoma (IDC; n=72; P=.014) compared with other histological type (n=29), and TL in IDC with HER2 negative (n=53; P=.017) was higher than TL in IDC with HER2 positive (n=19). However, telomeres were shortened in advanced stages and growing tumors. IGFBP7 methylation was observed in 90% of tumor tissues and 59% of controls (P=.0002). Its frequency was significantly higher in IDC compared with invasive mixed carcinoma (IMC; P=.002) and it was not correlated either with protein expression or the other clinicopathological parameters. Conclusion: These results suggest that IGFBP7 promoter methylation and shorter TL in tumor compared with adjacent tissues may be predictive biomarkers for breast cancer. Telomere maintenance may be indicative of IDC and IDC with HER2 (−) of breast cancer. Further studies with larger number of cases are necessary to verify this association.

  • 
  AtıfScopus6·WoS6·OpenAlex6

  İncele
• MakaleAçık Erişim2016Scopus SJRQ3/r/bayburt/13893
  Correlation between the DNA methylation and gene expression of IGFBP5 in breast cancer

  Karabulut, Sevgi; Kaya, Zehra; Amuran, Gökçe Gullu; Peker, Irem; Özmen, Tolga; Gulluolu, Bahadir M.; Kaya, Handan; Erzik, Can; Ozer, Ayşe; Akkiprik, Mustafa

  BACKGROUND: The insulin-like growth factor binding protein5 (IGFBP5) is often dysregulated in human cancers and considered neither a tumor suppressor nor an oncogene. OBJECTIVE: We aim to examine the reason of the changeable gene regulation of IGFBP5 in the case of methylation in breast cancer. METHODS: We used methyl-specific polymerase (MSP) chain reaction to detect CpG methylation of IGFBP5 promoter and exon-I in breast cancer and adjacent tissues. Gene expression is evaluated by quantative polymerase chain reaction (qPCR). RESULTS: IGFBP5 methylation was detected in 24 of 58 (41%) and 54 of 56 (96.5%) promoter and exon-I site respectively in tumor tissues. In adjacent tissues 17 of 58 (29%) and 53 of 56 (96.5%) was methylated. IGFBP5 expression was higher estrogene receptor (ER)(+) than ER(-) patients (p = 0:0549). Beside, we found a positive correlation between the expression of IGFBP5 and G2 tumor grade (p = 0:0131). However, no correlation was observed between IGFBP5 expression and age, menopause or the presence of lymph node metastasis (p > 0:05). CONCLUSIONS: In summary, our results showed that IGFBP5 promoter and exon-I methylation did not have any differences between tumor and adjacent tissues so that IGFBP5 methylation did not change IGFBP5 gene regulation in breast cancer. This is the first study investigating the IGFBP5 gene methylation in breast cancer.

  AtıfScopus9

  İncele