Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2025Scopus SJRQ3/r/bayburt/11799
  Chemical composition of essential oils and comparative analysis of enzyme inhibitory, antioxidant and antimicrobial activities in essential oils of endemic Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian

  Fidan, Muhammed Said; Baltacı, Cemalettin; Akmeşe, Osman; Sefalı, Abdurrahman; Aygül, İmdat; Öz, Mehmet

  In this study, essential oil from the aerial parts of Gelasia sericea (Aucher ex DC.) Zaika, Sukhor. & N. Kilian was extracted via hydrodistillation using a Clevenger apparatus. GC-MS/FID analysis determined aldehydes (28.31%) as the dominant group, with eucalyptol (8.71%) as the main constituent. Antioxidant activities were exhibited using DPPH, ABTS, and FRAP assays, with the essential oil demonstrating relatively higher antioxidant properties. The essential oil also showed higher levels of bioactive components, including total antioxidant capacity (160.26 mg AA eq./100 mL), total phenolic content (21.57 mg GA eq./100 mL), and total flavonoid content (14.85 mg QE eq./100 mL). While G. sericea essential oil demonstrated some antioxidant activity, it was significantly lower compared to the reference standard ascorbic acid (AA). The antimicrobial results showed relatively higher antimicrobial activity compared to most bacterial and fungal strains, as evidenced by lower MIC and MBC values and larger inhibition zones. Nevertheless, chloramphenicol (CH) and nalidixic acid (NA) continually showed superior antimicrobial efficacy, with the lowest MIC and MBC values and the largest inhibition zones. The essential oil also demonstrated potent enzyme inhibitory activity, especially against CA-II, AChE, and BChE, with the lowest IC50 value observed for CA-II, highlighting its potential as a potent enzyme inhibitor. However, the essential oil exhibited comparatively greater inhibition against AChE, BChE and α-amylase but was less effective overall. These findings suggest that the G. sericea essential oil has important antioxidant potential and enzyme inhibitory activity, while the essential oil shows varying efficacy across different enzymes.

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  AtıfOpenAlex1

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• MakaleYalnız künye2024Scopus SJRQ3/r/bayburt/11628
  Microwave hydrodistillation of Pelargonium graveolens L’Her leaves: Essential oil profile, phytochemical composition of wastewater, histo-anatomical structure

  Boztaş, Kadir; Gercek, Yusuf Can; Türer, Gizem; Canlı, Deniz; Bayram, Sinan; Çelik, Saffet; Kırkıncı, Seran; Basturk, Fatma Nur; Feizollahi, Sepideh; Cevahir Öz, Gül

  By-products with remarkable bioactive properties are formed in extraction processes of essential oils from plant materials. In present study, it was aimed to determine the concentration of individual phenolic and organic acid compounds by LC-MS/MS of the by-product (wastewater) collected after the essential oil isolation from Pelargonium graveolens L. and to determine some bioactive properties of this by-product. In addition, the chemical analysis and antibacterial activity of the essential oil extracted from P. graveolens were determined and the histo-anatomical characterization of the glandular trichomes in the leaf sample was performed. As a result, quercetin (95862.22 μg/kg), myricetin (59834.32 μg/kg) and rutin (36522.90 μg/kg) were the phenolic compounds determined in the highest concentrations in the by-product extracted using two different extraction techniques (non-hydrolysis and hydrolysis methods). Moreover, the individual organic acids of the by-product screened in LC-MS/MS was found to be 4487.17 mg/kg in total. When the essential oil component profile of the plant sample was examined, the citronellol (38.78%) compound was found to be dominant, and it was also detected that the essential oil had remarkable bioactive properties. In conclusion, it can be stated that both the essential oil obtained from P. graveolens and the by-product obtained during essential oil isolation can be considered as a source of some bioactive components in many different industrial applications.

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  AtıfScopus6·WoS4·OpenAlex6

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• MakaleAçık Erişim2022Scopus SJRQ2/r/bayburt/13493
  Characterization of Essential Oil and Wastewater from Thymus nummularius M. Bieb. and Micromorphological Examination of Glandular Trichomes

  Gerçek, Yusuf Can; Bayram, Sinan; Çelik, Saffet; Canlı, Deniz; Mavaldi, Muhammed Halid; Boztas, Kadir; Bastürk, Fatma Nur; Kırkıncı, Seran; Yesil, Yeter; Kösesakal, Taylan

  This study aimed to determine the volatile profile by GC-MS and antimicrobial activity of the essential oil isolated from Thymus nummularius using the microwave-assisted hydrodistillation method and morphologically characterize the glandular trichomes in leaf samples. Obtained essential oil, as well as wastewater obtained during essential oil isolation, were chemically (polyphenolic compounds, organic acids, total phenolic content, total flavonoid content and antioxidant activity) characterized. As a result of GC-MS analysis of the essential oil, different ratios of essential oil components were identified, predominantly thymol (38.91%), linalool (13.12%) and geraniol (6.51%). The antimicrobial activity results showed that T. nummularius essential oil has a broad-spectrum inhibitory effect against all target microorganisms (bacteria and yeast-like fungi). Screening of 32 polyphenolic compounds and 52 organic acids in the wastewater was performed by LC-MS/MS and the major polyphenolic compound and organic acid were determined as caffeic acid (97316.85 µg/kg) and malic acid (6898.88 mg/kg), respectively. While the total phenolic and flavonoid content of the wastewater were 35.91 mg GA/g and 34.91 mg QE/g, respectively, the antioxidant activity results were obtained using the CUPRAC, CERAC and ABTS tests were 86.28 mg TE/g, 72.85 mg TE/g and 47.88 mg TE/g. These results support that the T. nummularius, an important production area in Turkey, can be used in different industrial areas due to its rich bioactive content. In addition, it can be said that wastewater is also considered a valuable source of bioactive substances.

  AtıfScopus11·WoS8·OpenAlex11

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• MakaleAçık Erişim2013Scopus SJRQ4/r/bayburt/13739
  Protective Role of Mentha longifolia L. ssp. longifolia against Aflatoxin B

  Ceker, Selcuk; Agar, Guleray; Alpsoy, Lokman; Nardemir, Gokce; Kizil, Hamit Emre; Mete, Ebru

  The aim of this study was to investigate the effects of essential oil of Mentha longifolia L. ssp. longifolia, used since ancient times for the treatment of many digestive tract diseases, on the genotoxicity and oxidative stress by a single dose of Aflatoxin B1 (5 μM) in the human lymphocyte culture. The total of the 12 identified components accounted for 97.69 % by Gas chromatography (GC). Major components of the oil were cis-piperitone epoxide (26.52 %), piperitenone oxide (26.40 %), pulegone (15.66 %) and piperitenone (13.57 %). According to the results obtained from GC-MS systems, monoterpenes consist of 89.19 % of the total oil approximately while sesquiterpenes were represented in small quantities in the oil. We studied the anti-genotoxic effects of essential oils of M. longifolia (MEO) by using sister chromatid exchange (SCE) and micronucleus (MN) test systems. Also, the anti-oxidative effects of MEO were evaluated by using superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) assay. The SCE frequency and the number of MN were increased when treated with AFB1. Especially, MEO at 1.5 and 2.0 μl reduced SCE frequency and MN number in the human lymphocyte (p <0.05). In addition, AFB1 treatment significantly reduced the activity of SOD and GPx and elevated the level of MDA (p<0.05). MEOs treatment led to significant attenuation of AFB1-induced oxidative stress by normalization of the activities of SOD and GPx and the MDA (p < 0.05). These results suggest that MEOs could provide anti-oxidative and anti-genotoxic protection for the oxidative and genotoxic agents that cause many diseases including cancer and neurodegenerative disease.

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  AtıfScopus10·WoS11·OpenAlex11

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• MakaleYalnız künye2012Scopus SJRQ4/r/bayburt/13995
  Investigation of anti-oxidative and anti-genotoxic effects of Origanum vulgare L. essential oil on human lymphocytes in vitro

  Ceker, Selcuk; Agar, Guleray; Nardemir, Gokce; Anar, Mustafa; Kizil, Hamit Emre; Alpsoy, Lokman

  Origanum vulgare L. (Lamiaceae) is a flavoring herb widely using around the world. Some preparations and extracts from O. vulgare have been used in Turkish traditional medicine to treat several ailments including digestive disorders and menstrual problems. This study is designed to examine the chemical composition and in vitro antigenotoxic and antioxidant activity of the essential oil of O. vulgare (OVE) from Turkey. The essential oil of O. vulgare was analized by using GC. OVEs that were investigated antagonistic effects against oxidative stress and the genotoxic effects of aflatoksin B1 (AFB1). Anti-genotoxic activity was evaluated by sister chromatid exchanges (SCEs) and micronucleus (MN) tests. In additional to investigating protective role of OVEs against the oxidative stress of AFB1 in vitro, oxidative status was assessed on erythrocytes by measuring following oxidative stress markers like; super oxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA).The total of the 25 identified components are accounted for 96.03 % (GC). Major components of the oil were β-caryophyllene (20.40%), and caryophyllene oxide (34.44%). In the SCE and MN tests, it was observed that OVEs suppressed the mutagenic effects of AFB1. OVEs were observed to modulate the adverse effects of AFB1. The results of this experiment clearly reveal that OVEs have strong anti-oxidative and anti-genotoxic effect. These anti-genotoxic effects of O. vulgare essential oils are probably related to its action on the enzymatic activation system. Anti-oxidative and anti-genotoxic effect may be role of these compounds (β-caryophyllene and caryophyllene oxide).

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  AtıfScopus14·WoS16·OpenAlex12

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