Sürdürülebilir Kalkınma Amaçları

• 3
• 1

• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/10771
  Optimization of Ethanol Extraction Conditions From Sun-Dried Apricot and Prediction of Antioxidant, Phenolic, and Flavonoid Contents and Their Effects on HCT116 Cells Using Artificial Neural Networks

  Topcu, Kubra Cinar; Gueven, Sara Altun; Cakir, Ozlem; Anlar, Pinar; Durul, Melekber Sulusoglu; Ay, Mizgin; Alyafei, Mohammed; Hayat, Faisal; Ercisli, Sezai

  This study aimed to optimize the ethanol extraction conditions of sun-dried apricot (Prunus armeniaca L.) and evaluate the antioxidant capacity, phenolic, and flavonoid contents of the obtained extracts. Response surface methodology (RSM) was applied to determine the optimal extraction conditions, which were identified as 60 degrees C temperature, 34% ultrasonic power, 46 min sonication time, and a 4 g/mL solid-liquid ratio. Under these conditions, the extract exhibited a total phenolic content (TPC) of 4.20 mg GAE/g, a total flavonoid content (TFC) of 7.09 mg QE/g, a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of 1.37 mg TE/g, a ferric reducing antioxidant power (FRAP) value of 9.12 mg TE/g, and a thiobarbituric acid reactive substances (TBARS) level of 1.69 mg MDA/g. The artificial neural network (ANN) model provided highly accurate predictions for these parameters. Additionally, cell culture experiments demonstrated that the extract exerted a dose-dependent cytotoxic effect on HCT116 colon cancer cells, significantly reducing their viability. These findings highlight the potential of sun-dried apricot extracts as natural antioxidants with possible applications in the functional food and pharmaceutical industries.

  • 
  AtıfScopus2·WoS2·OpenAlex2

  İncele
• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/12760
  Pharmacological Potential and Bioactive Components of Wild Anatolian Sage (Salvia aethiopis L.)

  Bilginoğlu, Emine; Kızıl, Hamit Emre; Öğütcü, Hatice; Ağar, Güleray; Bağcı, Yavuz

  Salvia aethiopis L., a member of the Lamiaceae family, has gained attention due to its bioactive compounds with potential therapeutic applications. This study investigated the chemical composition and biological activities of S. aethiopis ethanol extract collected from Karaman province, Türkiye. The phenolic profile of the plant was characterized using LC–MS/MS analysis, which revealed substantial amounts of rosmarinic acid, ferulic acid, caffeic acid, and p-coumaric acid. The antimicrobial activity of the extracts was evaluated against various Gram-positive and Gram-negative bacteria, as well as fungal strains, using the agar well diffusion method. The results demonstrated potent antibacterial effects, particularly against Staphylococcus aureus, Micrococcus luteus, and Klebsiella pneumoniae. Furthermore, the cytotoxic potential of S. aethiopis was assessed on H-460 non-small cell lung cancer cells. The extract exhibited a dose-dependent cytotoxic effect, with a significant reduction in cell viability after 24 and 48 h of treatment, yielding IC50 values of 80.08 and 56.19 μg/mL, respectively. These findings suggest that S. aethiopis possesses promising antimicrobial and anticancer properties, which could contribute to the development of novel therapeutic agents.

  • 
  AtıfScopus8·WoS7·OpenAlex7

  İncele
• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/11134
  Dietary Carvacrol Attenuates Cyclophosphamide-Induced Neurotoxicity: Implications for Food-Derived Neuroprotection and Molecular Mechanisms

  Kızıl, Hamit Emre

  Carvacrol (CRV) is a phenolic monoterpene abundant in culinary herbs such as oregano and thyme and is well known for its potent antioxidant, anti-inflammatory, and neuroprotective properties. This study investigated the ability of CRV to counteract neurotoxicity induced by cyclophosphamide (CP), a widely used antineoplastic agent. Male Wistar albino rats were divided into five groups and received CP and/or CRV treatments. Neurotoxicity and neuroprotection were evaluated through biochemical assays, real-time PCR, histopathological and immunohistochemical analyses, and behavioral testing (Morris Water Maze). CP administration led to significant increases in oxidative stress markers, disruption of antioxidant enzyme activities, upregulation of inflammatory mediators (NF-κB, TNF-α, iNOS), dysregulation of apoptotic regulators (increased Bax and Casp-3, decreased Bcl-2), alterations in autophagy markers (Beclin-1, LC3A, LC3B), and suppression of Notch1/Hes1 signaling. Histopathological analyses revealed neuronal degeneration, vascular hyperemia, and increased GFAP and 8-OHdG expression in brain tissue. CRV treatment, particularly at higher doses, effectively mitigated these biochemical, molecular, and histological alterations. Notably, CRV administration preserved spatial learning and memory function in CP-treated rats, as demonstrated by the Morris Water Maze test, indicating functional neuroprotection. These findings highlight the multifaceted neuroprotective mechanisms of CRV and suggest its potential as a food-derived bioactive compound for development into functional foods or dietary supplements to improve the quality of life for cancer patients undergoing chemotherapy.

  • 
  AtıfScopus10·WoS9·OpenAlex11

  İncele
• MakaleYayıncıda açık erişim2024Scopus SJRQ1/r/bayburt/11767
  Can Enzymatic Modified Pectin in Mediterranean Fruits Be Therapeutic Against Stomach Cancer?

  Üstün, Selime; Kizil, Hamit Emre; Dertli, Enes

  The aim of this study was to evaluate the cytotoxic effects of enzymatically modified pectin products derived from grapefruit, jujube, and kumquat on the MKN-45 gastric cancer cell line in vitro. FTIR analysis revealed that the spectra of the pectins produced were comparable to those of commercial pectin and included the characteristic peaks identified in the literature. The galacturonic acid content was measured as 612 mg/g in grapefruit pectin, 544 mg/g in jujube pectin, and 704 mg/g in kumquat pectin. DSC analysis indicated that all pectin samples and their modifications exhibited one endothermic peak and one exothermic peak. Cytotoxicity assessments revealed that the enzymatically modified grapefruit pectin demonstrated significant cytotoxic effects across all tested concentrations, with 0.075 mg/mL being the most effective. For kumquat pectin, all tested concentrations showed cytotoxic properties, with 0.3, 0.15, and 0.075 mg/mL being the most effective. In the case of jujube pectin, cytotoxic effects were observed at all concentrations except for 1.2 mg/mL.

  • 
  AtıfScopus3·WoS3·OpenAlex3

  İncele