Sürdürülebilir Kalkınma Amaçları

• 1

Yazarlar

• Dertli, Enes(3)
• İspirli, Hümeyra(2)
• Ahn-Jarvis, Jennifer H.(1)
• Alkay, Zuhal(1)
• Arioglu-Tuncil, Seda(1)
• Balubaid, Mohammed(1)
• Bayaman, Rabia Yusra(1)
• Can, Hasan(1)
• Dere, Sevda(1)
• Gonzales, Miguel Angel Alvarez(1)
• Harris, Hannah C.(1)
• Ispirli, Humeyra(1)
• Kabli, Mohammad(1)
• Lindemann, Stephen R.(1)
• Ozulku, Gorkem(1)
• Sagdic, Osman(1)
• Savva, George M.(1)
• Taylan, Osman(1)
• Tuncil, Yunus Emre(1)
• Warren, Frederick J.(1)
• Yılmaz, Mustafa Tahsin(1)

• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/11035
  The prebiotic potential of alternansucrase-based glucooligosaccharides: the effects on short-chain fatty acids production and microbiome diversity

  Ozulku, Gorkem; Harris, Hannah C.; Savva, George M.; Ahn-Jarvis, Jennifer H.; İspirli, Hümeyra; Dertli, Enes; Warren, Frederick J.

  Glucooligosaccharides (GLOS) produced by glucansucrases via acceptor reactions are oligosaccharides that transit upper intestinal tract and evade digestion and may be considered to be a prebiotic. The prebiotic function of GLOS can be dictated by the type of acceptor sugar used during its production. Therefore, the prebiotic effect on the gut microbiome and short chain fatty acids (SCFAs) production can be used to inform the selection of acceptor sugars. In this study, alternansucrase-based GLOS derived from different acceptor sugars (melibiose, cellobiose and lactose) and inulin (positive control) were investigated using in vitro colon model fermentation to examine their prebiotic effect and SCFAs production. Microbiome composition was determined using shotgun metagenomics. SCFAs and lactate formation was determined using 1H NMR spectroscopy. Fusicatenibacter saccharivorans and Bacteroides uniformis appeared to increase in abundance after 24 h following fermentation of all three GLOS. The highest abundance for Bifidobacterium longum was seen after supplementation with lactose derived GLOS. Fermentation of GLOS appeared to produce around twice as much acetate than fermentation with inulin, while similar amounts of other SCFAs and lactate were produced. Melibiose derived GLOS supplementation in healthy 3 donors increased F. saccharivorans while inulin supplementation promoted Lactobacillales in order level. This study suggests that there may be beneficial effects of GLOS's on gut microbiome composition and metabolite composition, relative to the effects seen for inulin.

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  AtıfScopus1·OpenAlex1

  İncele
• MakaleAçık Erişim2025Scopus SJRQ1/r/bayburt/10808
  Characterizations and In Vitro Gut Microbiome Modulatory Effects of Gluco-Oligosaccharides Synthesized by the Acceptor Reactions of Glucansucrase 53

  Bayaman, Rabia Yusra; Alkay, Zuhal; Ispirli, Humeyra; Arioglu-Tuncil, Seda; Dere, Sevda; Can, Hasan; Gonzales, Miguel Angel Alvarez; Sagdic, Osman; Lindemann, Stephen R.; Tuncil, Yunus Emre

  The production of novel oligosaccharides with potential prebiotic effects is of interest to expand the current market and explore the effectiveness of new functional carbohydrate forms. The utilization of glucansucrases is a cost-effective and environmentally friendly biotechnological strategy for producing novel gluco-oligosaccharides through acceptor reactions. In this study, an active glucansucrase (GS53) was used to produce gluco-oligosaccharides via its acceptor reactions with glucose, maltose, and maltotriose, and these oligosaccharides were tested in terms of structure and their gut microbiome modulatory effects. The formations of oligosaccharides were monitored by TLC analysis, and GS53 was active for the three acceptors but not for the other sugars tested. The structural characterization of the gluco-oligosaccharides by 1H NMR analysis revealed the glycosylation of each acceptor with alpha-(1 -> 3) and alpha-(1 -> 6) linkages, whereas LC-MS analysis demonstrated the formations of DP 8, DP 7, and DP 6 oligosaccharides with acceptors maltose, maltotriose, and glucose, respectively. In vitro fecal fermentation analysis, in which microbial short-chain fatty acids (SCFAs) and microbial compositional changes were assessed using gas chromatography and 16S rRNA sequencing, respectively, demonstrated that the gluco-oligosaccharides formed SCFAs-particularly propionate and butyrate-at levels comparable to those observed with inulin, a well-established prebiotic. Additionally, the gluco-oligosaccharides were found to promote the growth of Bifidobacterium adolescentis and Blautia OTUs, which are known to have important physiological functions beneficial to human health. Overall, these results demonstrate that gluco-oligosaccharides synthesized using GS53 through acceptor reactions exhibit prebiotic potentials and could be utilized in the future as dietary supplements as well as in the development of functional foods targeting colonic health.

  AtıfScopus3·WoS3·OpenAlex3

  İncele
• MakaleAçık Erişim2022Scopus SJRQ3/r/bayburt/13478
  Optimization of lactose derivative hetero-oligosaccharides production using whey as the acceptor molecule by an active glucansucrase

  Kabli, Mohammad; İspirli, Hümeyra; Balubaid, Mohammed; Taylan, Osman; Yılmaz, Mustafa Tahsin; Dertli, Enes

  Glucansucrases are capable of synthesis of distinct oligosaccharides using different acceptor molecules and in this study lactose derivative hetero-oligosaccharides were shown to be produced by an active glucansucrase in its acceptor reaction with sucrose and whey as the donor-acceptor molecules, respectively. Following the monitoring of the reaction products by TLC analysis, the effects of reaction parameters in terms of enzyme concentration, sucrose/whey ratio and reaction temperature on final oligosaccharide yields (OY) were optimized by Response Surface Methodology (RSM). Results indicated that increment of enzyme concentration and reaction temperature decreased the OY in a particular reaction time whereas the OY increased with the increment of sucrose/whey ratio in the acceptor reactions of glucansucrase E81 with whey as the acceptor molecule. A low level of glucansucrase E81 concentration was sufficient to obtain the highest OY in the tested conditions. These findings demonstrated the importance of the optimisation of the reaction parameters for the acceptor reactions of the glucansucrases.

  AtıfScopus2·WoS4·OpenAlex6

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