Sürdürülebilir Kalkınma Amaçları
Yazarlar
Yayınlar
- MakaleAçık Erişim2023Analysis of telomere length in patients with COVID-19 and investigation into its relationship with clinical- demographic data
Savrun, Atakan; Dirican, Ebubekir
Purpose: Novel coronavirus disease 2019 (COVID-19) is an infectious disease unknown before the 2019 outbreak in Wuhan. This study evaluated telomere length in COVID-19 (+) and (-) samples with clinical-demographic parameters.Materials and Methods: DNA was isolated from COVID-19 (+) (n=70) and (-) (n=70) patients. Telomere length was determined by real-time-PCR (RT-PCR). The 2- increment increment Ct method was used to analyze the telomere length of the samples.Results: There were significant differences in creatinine, LDH, ferritin, WBC, NEU and CRP in COVID-19 (+) patients compared to COVID-19 (-) patients. The NEU/LYM (or N/L) ratio was found higher in the patients with COVID-19 (+), than in COVID-19 (-). On the other hand, our COVID-19 (+) patients (mean +/- std:0.93 +/- 0.58) had significantly shorter telomere lengths than the COVID-19 (-) (mean +/- std:1.26 +/- 0.76). Moreover, COVID-19 (+) male patients (mean +/- std:1.06 +/- 0.50) had longer telomere length than female patients (mean +/- std:0.76 +/- 0.54). Telomere length was significantly shorter in patients with COVID-19 (+) with high blood urea nitrogen (BUN), high creatinine, high hematocrit, high NEU levels, normal platelets (PLT), and low WBC levels.Conclusion: Our findings suggest that telomere length and blood parameter levels influence the severity of COVID-19. Blood parameters differed in patients with COVID-19 (+) and COVID-19 (-). As a result, increasing the number of similar studies in the future can demonstrate the significance of our findings.
AtıfWoS2OpenAlex2
İncele - MakaleAçık Erişim2022Investigation of the effect of gossypin on MMP-2 and MMP- 9 genes in prostate cancer cells
Dirican, Ebubekir; Çinar, Irfan
Purpose: The aim of this study is to explore the effects of gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials and Methods: PC3 cells were grown in culture and treated with three different concentrations of gossypin (25-50-100 mu g/ml) and cisplatin (50 mu M) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis was performed and RT-PCR was used to evaluate mRNA expression levels of MMP-2 and MMP-9 genes. Results: Gossypin decreased MMP-2 and MMP-9 mRNA expression in prostate cancer cells in a concentrationdependent manner. Three concentrations (25-50-100 mu g/ml) of gossypin in PC3 cells reduced the mRNA expression of the MMP-2 gene. While the fold change value of MMP-2 gene expression was 0.3482 +/- 0.040 in the 100 mu g/ml gossypin group, it was 1.007 +/- 0.1425 in the control group. In addition, 50 mu g/ml and 100 mu g/ml concentrations of gossypin decreased the mRNA expression of the MMP-9 gene. The expression level of the MMP-9 gene in prostate cancer cells was 0.4740 +/- 0.038 in the 100 mu g/ml gossypin administered group, while it was 1.009 +/- 0.1687 in the control group. There was a positive correlation between the expressions of the MMP-2 and MMP-9 genes. Conclusion: According to the results obtained, it is seen that gossypin reduces the expression of MMP-2 and MMP9 genes in prostate cancer cells and the effects of gossypin on other genetic and epigenetic mechanisms in cancer need to be investigated to reveal the anti-cancer.
AtıfWoS2OpenAlex2TR Dizin1
İncele