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- MakaleAçık Erişim2024Scopus SJRQ2Determination of anti-cancer effects of Nigella sativa seed oil on MCF7 breast and AGS gastric cancer cells
Çınar, İrfan; Gıdık, Betül; Dirican, Ebubekir
Background: This study aimed to investigate the cytotoxic, apoptotic, invasion, metastasis, and heat shock proteins (HSPs) effects of N. sativa oil on breast and gastric cancer cells. Methods: We assessed the cytotoxic and apoptotic effects of various concentrations of N. sativa oil (10-50-100-200 µg/mL) on MCF7 breast cancer and AGS, an adenocarcinoma of the gastric cell line, at 24, 48 and 72 h using the MTT test. Additionally, the expression of the Caspase-3, BCL2/Bax, MMP2-9 and HSP60-70 gene was examined using RT-PCR in cell lines treating with N. sativa. Results: The MTT experiments demonstrate that N. sativa has a time and dose-dependent inhibitory effect on the proliferation of MCF7 and AGS cancer cells. The vitality rates of MCF7 and AGS cells treated with N. sativa were 77.04–67.50% at 24 h, 65.28–39.14% at 48 h, and 48.95–32.31% at 72 h. The doses of 100 and 200 µg/mL were shown to be the most effective on both cancer cells. RT-PCR analysis revealed that N. sativa oil extract increased caspase-3 levels in both cell lines at higher concentrations and suppressed BCL2/Bax levels. Exposure of MCF7 and AGS cell lines to N. sativa caused a significant decrease in the expression of MMP2-9 and HSP60-70 genes over time, particularly at a dosage of 200 µg/mL compared to the control group (p < 0.05). Conclusions: Our findings indicate that N. sativa oil has a dose-dependent effect on cytotoxicity and the expression of apoptotic, heat shock proteins, and matrix metalloproteinases genes in breast and gastric cancer.
AtıfScopus11WoS6OpenAlex12
İncele - MakaleAçık Erişim2022Impact of gossypin on gene expression of HSP60 and HSP70 in different cancer cell lines
Dirican, Ebubekir; Çinar, Trfan
Purpose: The aim of this study is to evaluate the impact of gossypin on the expression level of heat shock proteins (HSPs) genes in different cancer cells. Materials and Methods: Cells were grown under standard culture conditions. Cancer cells were treated with different concentrations (5-100 mu g/ml) of gossypin and cisplatin (50 mu M) as positive control. Cell viability and effective dose range (5-100 mu g/ml) of gossypin were determined by MTT at 24, 48 and 72 hours. After RNA isolation and cDNA synthesis, HSP60 and HSP70 gene expression levels were analyzed using RT-PCR. For gene expression analysis, the 2-(Delta Delta ct) method was used. Results: According to the Mil results, 25-50-100 mu g/ml of gossypin doses were found effective on HSP60 and HSP70 gene expression levels in the cancer cell lines. Gossypin affected with dose-dependently the expression of HSP60 and HSP70 in the three cell lines. In the three cell lines, 50 mu g/ml and 100 mu g/ml of gossypin doses significantly reduced the expression of HSP60 and HSP70 compared to control group. Conclusion: Our results strongly supported the anticarcinogenic effect of gossypin at various doses in different cell lines. However, we believe that further in vivo research and human studies are needed. Our findings suggest that gossypin could be suitable candidate agent for further investigation to develop new strategies for the prevention and/or treatment of different cancer types.
AtıfWoS1OpenAlex2
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