Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2025Scopus SJRQ1Optimization of Hemp Seed (Cannabis sativa L.) Protein Extraction and Glycation Conditions by Response Surface Methodology: Physicochemical, Functional, and Thermal Characteristics
Yüzer, Elvan; Kutlu, Naciye; Yüzer, Mustafa Onur
In this study, first, optimization using the response surface method was implemented to obtain the highest yield of protein isolate from defatted hemp seed under different extraction conditions. Optimum extraction conditions were determined with 0% salt concentration, 12 pH value, and 49°C temperature. The protein isolate produced under optimum conditions was subjected to the glycation process, and the glycation conditions were also optimized. The optimum conditions for glycation were determined as a protein isolate: sugar ratio of 1.6:1, temperature of 60°C, and time of 90 min, providing the most suitable glycation degrees and browning indices. There was no statistical difference between the glycated protein isolate obtained under these conditions and the nonglycated protein isolate in terms of emulsifying activity index and emulsion stability index. For total phenolic–flavonoid compounds and antioxidant activity, the sugar complex caused a decrease in these values. In addition, there was a high positive correlation between bioactive compounds. SEM micrographs showed relationships between hemp seed protein isolate and sugar, and these interactions led to a spongy three-dimensional microstructure. When the FTIR results were analyzed, the glycated isolate had stronger absorption at 3300–3200 cm−1 compared to the nonglycated isolate. In conclusion, this study reports important findings in terms of functional and characteristic properties of how monosaccharides affect glycation.
AtıfScopus1WoS1
İncele - MakaleAçık Erişim2024Scopus SJRQ4Effect of Heat Treatment on Storage Stability of Sheep Tail Fat; [Isıl İşlemin Koyun Kuyruk Yağının Depolama Stabilitesi Üzerine Etkisi]
Bozdemir, Merve; Gençcelep, Hüseyin; Yüzer, Mustafa Onur
In this study, the effects of various physical, chemical, and technological properties of sheep tail fat (fresh and ghee) as well as different storage durations, storage temperatures, and the use of additives on the storage stability of thermally processed sheep tail fat (ghee tail fat) were investigated. To prevent lipid oxidation and other degradation factors during use after long-term storage, salt and antioxidant additives were used. Samples were stored in glass jars at 25ºC, +4ºC, and -18ºC for 90 days. The moisture, protein, fat, and ash contents of fresh tail fat was determined as 11.2%, 3.47%, 85.0%, and 0.07%, respectively. In clarified fat, the moisture content was determined as 0.4%, protein content as 2.98%, fat content as 96.0%, and ash content as 0.05%. Additionally, the cholesterol content in tail fat was 60 mg/100 g in fresh tail fat and 58 mg/100 g in ghee tail fat. The pH values of fats were determined as 5.7 in fresh samples and 6.2 in ghee samples. The fatty acid compositions of samples revealed that the dominant fatty acid groups in both fresh and ghee sheep tail fat were palmitic, stearic, and oleic acids. Fresh tail fat stored at room temperature deteriorated in terms of chemical properties (FFA, peroxide, and TBA) within 7 days, while those stored at +4ºC deteriorated within 15 days. However, thermally processed samples were preserved without deterioration for 60 days at these storage temperatures.
AtıfScopus1OpenAlex1İncele - MakaleAçık Erişim2023Soya proteini izolatı-fruktoz glikasyon konjugatlarının yapısı ve bazı teknofonksiyonel özellikleri üzerine ultrason işleminin etkisi
Yüzer, Mustafa Onur
Bu çalışmada, konjugatlar ultrasonik işlemden (130 W, 20 kHz) 5 ve 15 dakika sonra soya protein izolatı (SPI) ve fruktoz (F) sulu dispersiyonlarının ısıtılıp glikasyon reaksiyonu (95 oC, 30 dakika) gerçekleştirilmesiyle hazırlanmıştır. Ultrasonik ön işlemin, elde edilen SPI-F konjugatlarının yapı ve fonksiyonel özellikleri üzerindeki etkisi araştırılmıştır. Serbest amino grupları, aşılama derecesi ve FT-IR analizleri SPI-F konjugatlarının oluşumunu doğrulamıştır. Ultrasonik işlem glikasyon reaksiyonunu teşvik etmiştir. Konjugatların yapısal ve fizikokimyasal özelliklerindeki değişiklikler SPI’nin su tutma ve yağ tutma özelliklerinin düşmesine neden olsa da emülsifikasyon özelliklerini geliştirmiştir. Bu nedenle, emülsifikasyon özelliklerini kontrol etmek için soya proteini glikasyon konjugatlarının yapısını değiştirmek için optimum bir ultrasonik ön işlem kullanılabilir. Bu bulguların, gıda emülsiyonu ve diğer sistemlerde glikasyonla modifiye edilmiş protein-sakarit ikili sistemlerinin uygulanması için yararlı olacağı düşünülmektedir.
AtıfOpenAlex1TR Dizin2
İncele - MakaleAçık Erişim2022Kastamonu pastırmasının kalite özelliklerinin belirlenmesi: Kuru küredilmiş bir et ürünü
Gençcelep, Hüseyin; Yüzer, Mustafa Onur; İhtiyar, Barış
Pastırma, kendine has üretim teknolojisi ile yüzyıllardır üretilen bir Türk kuru et ürünüdür. Sığır ve manda karkaslarının belirli kısımlarından elde edilen tüm kas ve/veya kaslardan üretilir. Bu çalışma, Kastamonu pastırma türlerinden en çok üretimi yapılan sırt ve kuşgömü türlerinin bazı fizikokimyasal özelliklerini inceleyen ilk çalışmadır. Tüm analizlerde pastırma türleri arasında önemli farklılıklar tespit edilmiştir (P<0.05). Pastırma tipi nem miktarını önemli derecede etkilemiştir (P<0.05). Kuşgömü pastırmada ortalama nem içeriği sırt pastırmadan daha yüksek olarak belirlenmiştir. Tüm örneklerin toplam protein miktarı %30'un üzerinde belirlenmiş olup, beslenme açısından oldukça değerlidir. Ancak, numunelerin %50'sindeki tuz miktarı (%10 / kuru madde) ve pH (maks. 6.0) kabul edilebilir maksimum sınırın üzerindedir. Ayrıca tüm örneklerin su aktivite değerleri 0.90'ın üzerindedir. Bazı örneklerin TBARS ve FFA değerleri daha önce belirlenen değerlerden yüksek çıkmıştır.
AtıfOpenAlex6TR Dizin1İncele - MakaleAçık Erişim2020Scopus SJRQ1Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains
Purutoğlu, Kübra; İspirli, Hümeyra; Yüzer, Mustafa Onur; Serencam, Hüseyin; Dertli, Enes
Traditional kefir grains were collected from distinct parts of Turkey, and their microbial profile was determined. A wide bacterial biota was observed formed by distinct lactic acid bacteria (LAB) in which Lactococcus lactis strains appeared to be dominant. Yeast species were also identified in kefir grains. Significant levels of antifungal and antibacterial activities were monitored in kefir isolates. All tested LAB produced an exopolysaccharide (EPS) containing glucose and galactose, and some strains formed a fructan-type EPS. Importantly, low levels of antibiotic resistance were observed among the kefir isolates.
AtıfScopus37WoS32OpenAlex44İncele - MakaleAçık Erişim2019Scopus SJRQ3Characterization of a glucansucrase from Lactobacillus reuteri E81 and production of malto-oligosaccharides
İspirli, Hümeyra; Yüzer, Mustafa Onur; Skory, Christopher; Colquhoun, Ian J.; Sağdıç, Osman; Dertli, Enes
Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of α-glucans with different structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified and heterologously expressed. Alignments of GTFA with other glucansucrases revealed its novelty and a putative 3D model structure was obtained. The biochemical properties of the truncated enzyme without the N-terminal variable region, GTFA-ΔN, was characterized. The Km and Vmax were found to be 7.5 mM and 1.49 IU/mg, respectively, and it showed optimum activities at pH 7 and at 50 °C. The GTFA-ΔN produced in vitro an α-glucan with (α1 → 3) and (α1 → 6) glycosidic linkages using sucrose as the substrate. Importantly, GTFA-ΔN synthesized DP = 9 oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively, as detected by TLC, HPLC, LC-MS and NMR analysis.
AtıfScopus40WoS40OpenAlex40İncele - MakaleAçık Erişim2019Melissopalynology analysis, physicochemical properties, multi-element content and antimicrobial activity of honey samples collected from Bayburt, Turkey; [Türkiye'nin Bayburt Ilinden Toplanan Bal Örneklerinin Melissopalinolojik Analizi, Fizikokimyasal Özellikleri, Multi-Element Içerigi ve Antimikrobiyal Aktivitesi]
Ecem Bayram, Nesrin; Yüzer, Mustafa Onur; Bayram, Sinan
The aim of this study was to determine the plant sources, physicochemical properties, element content and antimicrobial effects of honey samples collected from 10 different regions of the province of Bayburt, Turkey. The melissopalynological analysis showed that the pollen samples of 67 plant taxa belonged to 34 plant families in honey samples and were found with different rates and TPN-10 values was found between 16024 and 90126. In addition to these, the amount of ash was between 0.13% and 0.32%, the electrical conductivity value was between 0.36 and 0.69 mS/cm, the moisture content was between 16.1% and 18.9% and the fructose/glucose ratio was between 0.92 and 1.18. As a result of physicochemical analysis, it was determined that the data obtained were in compliance with the standard values defined in by the Turkish Food Codex Communiqué on Honey (No: 2012/58). Elemental analysis performed with ICP-MS showed that the K element was the highest (261.34-1863.05 mg/kg) in all honey samples in total among the 42 elements. In addition to these, the antimicrobial effects of honey samples and minimum inhibition concentration values (MIC) were determined by the agar well diffusion (AWD) method and and microbroth dilution method respectively.
AtıfScopus11OpenAlex12TR Dizin7İncele