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- MakaleAçık Erişim2023Silver nanoparticle synthesis by biogenic reduction method and investigation of antimicrobial, antibiofilm, anticancer activities
Bülbül, Ali Savaş; Sen, Fatih; Bekmezci, Muhammed; Karakaya, Funda
In this study, R. aculeatus plant extract and biogenically formed AgNPs were investigated for their potential antibacterial, antibiofilm and anticancer abilities. AgNPs were characterised using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and UV-vis spectroscopy (UV-VIS). According to the Debye Scherrer formula, the particle size was found to be 32.56 nm. Disc diffusion and microdilution methods were used to investigate the antibacterial activity. In the disc diffusion study, the best results were obtained from the extract and AgNP. In the tests using plant extracts, Staphylococcus aureus ATCC 25923 showed the lowest antibiofilm activity, while Bacillus subtilis and Enterobacter aerogenes ATCC 13048 showed the highest activity. Salmonella infantis was most affected by AgNP, while Escherichia coli CFAI ATCC 25922 was least affected. Biogenically synthesised AgNPs were also investigated in cytotoxic activity studies. It was found to have the lowest concentration value on MCF-7 and HUVEC cell lines at the determined concentrations. The extract did not have any cytotoxic effect on MCF-7 cell line. HUVEC cell line showed the lowest cytotoxic activity of 10⁻³ g/mL. The antibacterial, antibiofilm and anticancer properties of R. aculeatus plant extract and biogenically produced AgNPs have been the subject of an important study. Furthermore, the comparison of the effects of plant extract and AgNPs on breast cancer cell lines and healthy cell lines provides a rich scientific material.
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İncele - MakaleAçık Erişim2023Antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of methanol extracts of some acantholimon boiss (plumbaginaceae) species
Bülbül, Ali Savaş; Sen, Fatih; Bostancı, Maşide Temiz; Uygun, Cihangir; Bekmezci, Muhammed; Bayat, Ramazan
Therapeutic properties of plants have been used since the existence of mankind. To benefit from this feature, the discovery of the active components of plants has gained momentum today. One of these plant species is Acantholimon Boiss. This plant extract has antifungal, antimicrobial, and anticancer properties. In this article, antimicrobial, antifungal and antioxidant capacities and antibiofilm properties of Acantholimon Boiss plant extract on 15 bacterial and 2 fungal species were evaluated. Antimicrobial properties were evaluated by Agar Well Diffusion method. It was observed that the plants formed a zone diameter of 12-18 cm. Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were used to determine the minimum active property. Biofilms of almost all bacteria were reduced by 10-50% by Acantholimon species at doses of 8 mg/ml-16 mg/ml. For MCF-7 cells, the IC50 values for A. acerosum subsp. brachystachyum (Boiss. ), A. acenum var. balansae, A. armenum var. balansae and A. kotschyi were 0.143 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml and 0.107 mg/ml, respectively. The same doses did not show a cytotoxic effect on HUVEC cell lines. With this article, it has given important results in terms of having a wide range of bacterial studies and examining its activities in fungi and cell lines. These findings suggest that extracts of Acantholimon Boiss species may be a potential source of natural medicines and antibacterial, antifungal, antibiofilm, antioxidant, and anticancer activities may support their therapeutic use.
- MakaleYalnız künye2022Scopus SJRQ1Investigation of antibacterial, antifungal, antibiofilm, antioxidant and anticancer properties of methanol extracts of Salvia marashica İlçim, Celep & Doğan and Salvia caespitosa Montbret & Aucher ex Benth plants with medicinal importance
Bostanci, Maside Temiz; Bulbul, Ali Savas; Celik, Ibrahim Seyfettin; Kocabas, Yusuf Ziya; Burhan, Hakan; Bayat, Ramazan; Sen, Fatih; Zakariae, Nilofar; Esmaeili, Roghayeh; Jafari, Hedayat
In this study, Antimicrobial, Antifungal and Antibiofilm activity tests on 16 bacteria and 2 fungi of Salvia marashica İlçim, Celep & Doğan and Salvia caespitosa Montbret & Aucher ex Benth species were performed by Agar Well Diffusion and Microdilution methods. Salvia species showed sensitivity with a zone diameter of 12–16 mm. Concentrations of 0.5 mg/ml to 16 mg/ml by the dilution method were used to determine the Minimum inhibited concentration (MIC) and Minimum bactericidal concentration (MBK). The MIC values of the plants are mostly 0.5 mg/ml - 4 mg/ml and MBC values are between 0.5 mg/ml - 8 mg/ml. Antifungal activity findings are remarkable on the species and it has been observed to have very high effects especially on Candida parapsilosis. When the antioxidant research findings are examined; It was observed that Salvia marashica and Salvia caespitosa plants have approximately 75% antioxidant activity at 1 mg/ml, and findings mostly directly proportional between concentrations and antioxidant activity capacity were recorded. MCF-7 and HUVEC cell lines were used to investigate anticancer activity properties. In line with the findings, while the IC50 value of Salvia marashica on the MCF-7 Cell line was 0.125 mg/ml, it was 1.65 mg/ml in the HUVEC cell line, while the IC50 value of Salvia caespitosa on the MCF-7 Cell line was 0.115 mg/ml in the HUVEC Cell line. It was found to be 9.87 mg/ml. It has been proven that both Salvia species have a cytotoxic effect on the MCF-7 Cell line.
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