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- MakaleAçık Erişim2022Clinicopathological Significance of hTERT gene expression in Breast Cancer Patients
Dirican, Ebubekir; Kankaya, Burak; Tatar, Zeynep Baran
Aim: This study aimed to examine the clinical significance of the mRNA expression of the human telomerase reverse transcriptase (hTERT) gene in breast cancer (BCa) and to investigate its clinicopathological role. The hTERT gene is involved in events such as cell proliferation, apoptosis and cell invasion. It has been reported that high hTERT mRNA expression is associated with poor prognosis in solid tumors such as BCa, stomach, lung, glioblastoma, head and neck. Material and Methods: RNA isolations of 59 BCa and healthy blood samples were performed. hTERT gene mRNA expression was analyzed by Real-Time-Polymerase Chain Reaction (RT-PCR) method. mRNA expression results were evaluated in cancer and healthy samples by the 2-∆∆ct method. Clinical data were collected and the relationship of these data with hTERT gene expression was evaluated statistically. Results: hTERT gene mRNA expression in BCa patients was significantly higher than in healty samples (p=0,0211). Low hTERT gene expression was significantly associated with estrogen receptor (ER) (+) and human epidermal growth factor receptor 2 (HER 2) (+) patients (p=0.0464; p=0.0080, respectively). hTERT gene expression was found to be higher in histological grade 3 patients compared to grade 2 (p=0.0389). Moreover, hTERT expression was shown to be significantly higher in basal-like histological type compared to luminal A and luminal B (p=0.0160; p=0.0008, respectively). Conclusion: The findings of our study showed that hTERT gene expression was significantly higher in BCa patients than in healthy samples. Abnormal hTERT gene expression may play an important role in carcinogenesis or diagnosis.
- MakaleAçık Erişim2020Scopus SJRQ4Investigation of alterations in PIK3CA and OCT-4 gene expression in breast cancer
Dirican, Ebubekir; Kankaya, Burak; Büyükaşık, Süleyman; Alış, Halil; Velidedeoğlu, Mehmet; İlvan, Sennur; İlvan, Ahmet
Breast cancer (BCa) is one of the most prevalent cancer amongst women and PIK3CA mutations have a crucial role in carcinogenesis. On the other hand, Octamer-Binding Transcription Factor 4 (OCT-4) is common biomarkers of cancer stem cells, which provide to the pathological processes of numerous carcinomas. In this study, we evaluated the association of OCT-4 gene expression levels with the PIK3CA mutation in BCa patients. The mRNA expression level of the OCT-4 gene was detected by qPCR in 44 BCa patients and 30 BCa patients control have positive PIK3CA mutations. Exon 9 and exon 20 of PIK3CA mutations were identified by sequencing. OCT-4 gene expression and PIK3CA mutations were also clinically evaluated in BCa patients. The frequency of PIK3CA mutation in 44 new cases were 32%. PIK3CA mutations were significantly associated with HER2-positive (p = 0.0271) status, Ki-67 status (p = 0.0434) and patient age (p = 0.0149). OCT-4 mRNA expression was significantly higher in the BCa tissue in comparison to normal breast tissue (p = 0.0305). A significant correlation was found between expression of OCT-4 and node status (p = 0.0190). OCT-4 mRNA expression was higher in the PIK3CA mutation tissue than the wild type tissue. However, no association was found between the expression of OCT-4 and the PIK3CA mutation or wild type samples (p = 0.7908). Our results provided that OCT-4 gene and PIK3CA mutations may have a role in development of BCa. OCT-4 mRNA expression level and PIK3CA mutations have a potential important for BCa diagnosis or treatment.
AtıfScopus1WoS1OpenAlex1
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