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- MakaleAçık Erişim2026Scopus SJRQ3Anatomical and Phytochemical Characterization of Ranunculus pinardii and Ranunculus constantinopolitanus: LC-MS/MS-Based Bioactive Compound Profiling
Feizollahi, Sepideh; Gercek, Yusuf Can; Canlı, Deniz; Basturk, Fatma Nur; Yesil, Yeter; Kösesakal, Taylan; Ecem Bayram, Nesrin
The aim of this study was to characterize Ranunculus pinardii and Ranunculus constantinopolitanus species anatomically and phytochemically. In order to contribute to the taxonomic characterization of R. pinardii and R. constantinopolitanus, anatomical structures of the stem, leaf, and petiole of species were examined with light microscopy. Total phenolic-flavonoid content, antioxidant activity, total anthocyanin, total proanthocyanidin, and individual phenolic compounds of extracts prepared with different solvents were determined. The results showed that the total phenolic contents of the species had a similar range (0.09-0.45 mg GAE/g for R. pinardii and 0.01-0.44 mg GAE/g for R. constantinopolitanus), but R. constantinopolitanus exhibited higher antioxidant activity (29.28 mg TE/g). Higher individual phenolic compound recovery was achieved from R. pinardii compared to R. constantinopolitanus with all solvents, except methanol. Both species were rich in luteolin, a natural flavonoid (163.98-34417.11 µg/kg for R. pinardii and 44.93-129009.44 µg/kg for R. constantinopolitanus). These results indicate that these plants contain bioactive ingredients that may have applications in different fields, such as medicine, cosmetics, and food. In particular, the detection of luteolin compound at high levels in these species compared to other components indicates that optimization studies aimed at the recovery of luteolin are important for future research.
- MakaleYalnız künye2024Scopus SJRQ3Microwave hydrodistillation of Pelargonium graveolens L’Her leaves: Essential oil profile, phytochemical composition of wastewater, histo-anatomical structure
Boztaş, Kadir; Gercek, Yusuf Can; Türer, Gizem; Canlı, Deniz; Bayram, Sinan; Çelik, Saffet; Kırkıncı, Seran; Basturk, Fatma Nur; Feizollahi, Sepideh; Cevahir Öz, Gül
By-products with remarkable bioactive properties are formed in extraction processes of essential oils from plant materials. In present study, it was aimed to determine the concentration of individual phenolic and organic acid compounds by LC-MS/MS of the by-product (wastewater) collected after the essential oil isolation from Pelargonium graveolens L. and to determine some bioactive properties of this by-product. In addition, the chemical analysis and antibacterial activity of the essential oil extracted from P. graveolens were determined and the histo-anatomical characterization of the glandular trichomes in the leaf sample was performed. As a result, quercetin (95862.22 μg/kg), myricetin (59834.32 μg/kg) and rutin (36522.90 μg/kg) were the phenolic compounds determined in the highest concentrations in the by-product extracted using two different extraction techniques (non-hydrolysis and hydrolysis methods). Moreover, the individual organic acids of the by-product screened in LC-MS/MS was found to be 4487.17 mg/kg in total. When the essential oil component profile of the plant sample was examined, the citronellol (38.78%) compound was found to be dominant, and it was also detected that the essential oil had remarkable bioactive properties. In conclusion, it can be stated that both the essential oil obtained from P. graveolens and the by-product obtained during essential oil isolation can be considered as a source of some bioactive components in many different industrial applications.
AtıfScopus6WoS4OpenAlex6
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