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- MakaleAçık Erişim2026Exploratory Proteomic Analysis of Rhododendron ponticum Bee Pollen Based on High-Resolution Mass Spectrometry
Ecem Bayram, Nesrin; Celik, Saffet
Bee pollen is a bee product that is widely preferred by people for its nutritional properties as well as its health-protective properties, and is defined as a ‘superfood’. In this preliminary exploratory study, monofloral Rhododendron ponticum bee pollen was analyzed using a label-free LC-QTOF-MS proteomic approach. According to the results obtained, a total of 37 proteins from the rhododendron plant have been fully confirmed in the pollen sample. Normalized spectrum counting values (NSAF) providing information about the semi-quantitative amount of these proteins were determined to be between 120.84 and 43259.22. Based on these findings, it can be said that the proteomic approach can provide biomarkers for monitoring the botanical source and quality of monofloral bee pollen. However, since no biological or technical replicate study was conducted, the findings obtained are exploratory in nature and should be considered semi-quantitative. To our knowledge, this study is the first to report the protein profile of R. ponticum bee pollen using a proteomic approach.
- MakaleAçık Erişim2022Scopus SJRQ1Screening of plant pollen sources, polyphenolic compounds, fatty acids and antioxidant/antimicrobial activity from bee pollen
Gercek, Yusuf Can; Celik, Saffet; Bayram, And Sinan
In this study, the botanical origin, total flavonoid and phenolic content, antioxidant activity, phenolic profile and fatty acid composition of mixed bee pollen loads collected in Bayburt, Turkey, were determined. In addition to these assays, antibacterial activity of bee-collected pollen extract (BCPE) against a variety of food-borne pathogenic bacteria was determined in vitro. Pollen loads were classified into five botanical families based on their color: Asteraceae, Fabaceae, Campanulaceae, Cistaceae and Rosaceae. Total flavonoid, total phenolic, CUPRAC and CERAC concentrations were 173.52 mg GAE/g, 79.21 mg QE/g, 85.59 mg Trolox/g and 118.13 mg Trolox/g, respectively. Twenty-three phenolic compounds were scanned in bee pollen extract by LC-MS/MS, with rutin being the most abundant. Cis-4,7,10,13,16,19 docosahexaenoic acid was the predominant fatty acid, followed by cis-11-eicosenoic acid, palmitic acid, and alfa linolenic acid. In addition, the agar well diffusion (AWD) and micro-broth dilution methods were used to determine of the antibacterial activity of the BCPE sample. MIC values were observed to vary between 2.5–5 mg/mL for Gram-positive bacteria and 5–10 mg/mL for Gram-negative bacteria. These findings indicate that bee pollen could be a potential source of antioxidants and antimicrobials.
AtıfScopus62WoS56OpenAlex71
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