Sürdürülebilir Kalkınma Amaçları
Yayınlar
- MakaleAçık Erişim2022Scopus SJRQ2Characterization of Essential Oil and Wastewater from Thymus nummularius M. Bieb. and Micromorphological Examination of Glandular Trichomes
Gerçek, Yusuf Can; Bayram, Sinan; Çelik, Saffet; Canlı, Deniz; Mavaldi, Muhammed Halid; Boztas, Kadir; Bastürk, Fatma Nur; Kırkıncı, Seran; Yesil, Yeter; Kösesakal, Taylan
This study aimed to determine the volatile profile by GC-MS and antimicrobial activity of the essential oil isolated from Thymus nummularius using the microwave-assisted hydrodistillation method and morphologically characterize the glandular trichomes in leaf samples. Obtained essential oil, as well as wastewater obtained during essential oil isolation, were chemically (polyphenolic compounds, organic acids, total phenolic content, total flavonoid content and antioxidant activity) characterized. As a result of GC-MS analysis of the essential oil, different ratios of essential oil components were identified, predominantly thymol (38.91%), linalool (13.12%) and geraniol (6.51%). The antimicrobial activity results showed that T. nummularius essential oil has a broad-spectrum inhibitory effect against all target microorganisms (bacteria and yeast-like fungi). Screening of 32 polyphenolic compounds and 52 organic acids in the wastewater was performed by LC-MS/MS and the major polyphenolic compound and organic acid were determined as caffeic acid (97316.85 µg/kg) and malic acid (6898.88 mg/kg), respectively. While the total phenolic and flavonoid content of the wastewater were 35.91 mg GA/g and 34.91 mg QE/g, respectively, the antioxidant activity results were obtained using the CUPRAC, CERAC and ABTS tests were 86.28 mg TE/g, 72.85 mg TE/g and 47.88 mg TE/g. These results support that the T. nummularius, an important production area in Turkey, can be used in different industrial areas due to its rich bioactive content. In addition, it can be said that wastewater is also considered a valuable source of bioactive substances.
AtıfScopus11WoS8OpenAlex11
İncele - MakaleYalnız künye2020Scopus SJRQ4Screening for antioxidant capacity, pollen types and phytochemical profile by GC/MS and UHPLC from propolis
Bayram, Nesrin Ecem; Gerçek, Yusuf Can; Öz, Gül Cevahir
Propolis is a resinous mixture collected by honeybees from different parts of plants such as poplars, birches, alders, conifers, pines, palms and willows. This study aims to determine pollen types (by light microscope), chemical compound profile (by GC-MS), fatty acid composition (by GC-MS), total phenolic content (by the Folin-Ciocalteu method), total flavonoid content (by the aluminium chloride method) and antioxidant capacity (by the CUPRAC, ABTS and CERAC methods) of a propolis sample from the western part of Istanbul, Turkey. As a result of microscopic analysis of the sample, pollen types of taxa belonging to 27 plant families were diagnosed. The GC-MS analysis of propolis revealed the presence of 38 phytochemical constituents that may contribute to its quality. Of these compounds, rates of "4H-1-benzopyran-4-one, 2,3-dihydro-5,7-dihydroxy- 2-phenyl-,(S)"were highest. In addition, the concentrations of naringenin, pinocembrin and galangin were determined to be 2.45, 9.92 and 7.06 mg/ml by UHPLC analysis. The extract had significant antioxidant activity in all assays, with values of 282.8±9 mg TE /g in the CUPRAC, 425.7±18 mg TE/g in the CERAC and 186.4±8 mg TE/g in the ABTS assays. Antioxidant capacity of the propolis extract was positively associated with the total phenolic and flavonoid contents of the extract. Moreover, the major fatty acids were C20:1n9 (cis- 11-eicosenoic acid), C22:1 (erucic acid) and C24:1 (nervonic acid). The results show that the propolis used in our study has important potential as an alternative food supplement and for cosmetic and therapeutic medicine and it can be used as an active agent in these areas.
AtıfScopus20WoS18OpenAlex3İncele - MakaleAçık Erişim2018Scopus SJRQ2Chemical characterization of 64 propolis samples from hakkari, Turkey
Bayram, Nesrin Ecem; Sorkun, Kadriye; Öz, Gül Cevahir; Salih, Bekir; Topçu, Gülaçtı
Propolis is a resinous substance produced by honey bees from various plants they visit. Sixty-four propolis samples were collected from different localities of four districts (Merkez, Yüksekova, Şemdinli, and Çukurca) in Hakkari territory. Ethanol extracts of the propolis samples were prepared, and their chemical contents were determined by gas chromatography–mass spectroscopy (GC–MS). Flavonoids were generally found in a high concentration in Şemdinli samples of Turkey. Twenty-seven coumarins were identified in the 28 propolis samples among studied 64 ones. Except suberosin, the coumarins were never reported before in any propolis sample with Turkish origin. The propolis samples belonging to Yüksekova were found to be richer in coumarins than the others, and the most richest one among the Yüksekova apiaries, was found to be Akocak sample (Y2) with 41.99% total yield, followed by Akçalı sample (Y5) (30.86%). This is a first comprehensive and original report about the chemical profile of propolis samples from Hakkari. The propolis samples from Hakkari exhibited a chemical content rich in flavonoids including coumarins and furocoumarins.
AtıfScopus16WoS14OpenAlex23İncele