Sürdürülebilir Kalkınma Amaçları

• 9

• MakaleYalnız künye2025/r/bayburt/14380
  Investigation of fatigue, mental well-being and tachycardia in short- and long-term metformin use

  Akeren, Zahide

  Purpose: The current study aimed compare short and long-term metformin users according to sociodemographic characteristics and to examine the relationships between fatigue, tachycardia and psychological well-being. Materials and Methods: In cross-sectional study were reached 92 patients, 46 short-term and 46 long-term metformin users. It was measured fatigue with the Functional Assessment of Chronic Illness Treatment Fatigue Scale (FACIT-F), tachycardia with saturation device, well-being with the World Health Organization Well-Being Index (WHO-5). Results: For the group using short-term metformin, the mean; year of metformin use was 1.82 +/- 0.77 and pulse rate was 82.48 +/- 8.80, FACIT-F score was 20.37 +/- 7.25, and WHO-5 score was 15.70 +/- 3.23. The group using long-term metformin mean year of metformin use was 5.93 +/- 2.68, pulse rate was 84.57 +/- 9.64, FACIT-F score was 25.33 +/- 5.65 and WHO-5 score was 12.63 +/- 3.70. Well-being negatively correlated with the year of diabetes, year of metformin use, pulse rate and fatigue. FACIT-F, metformin year and pulse rate explain a total of 41.9% of the variance in well-being. FACIT-F ((3=-.51, t=-5.82, p= .000) and the year of metformin use ((3=-.19, t=-2.12, p= .037) significantly decrease well-being, while pulse rate does not have a significant effect on well-being. Conclusions: Long-term use of metformin has been found cause fatigue and negative psychological well-being. Fatigue, year of metformin use and pulse rate number are important predictors of psychological well-being in Type II diabetes patients using metformin.

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• MakaleAçık Erişim2025/r/bayburt/14181
  Does Lrig1 expression have a relationship with ERBB1 and ERBB2 expression in schizophrenia?

  Uzunçakmak, Sevgi Karabulut; Özcan, Halil; Aydin, Pelin; Halici, Zekai

  Purpose: This study aims to investigate Lrig1 expression and to reveal the possible relationship between Lrig1, ERBB1 and ERBB2 in schizophrenia. Materials and Methods: In this study, peripheral blood samples of 70 schizophrenia patients and 60 healthy controls were used. Real Time PCR was applied for Lrig1, ERBB1 and ERBB2 gene expression analysis. Results: The level of Lrig1 mRNA was lower in the patients when compared to healthy controls. Expression levels of ERBB1 and ERBB2 were decreased in the patients versus in the healthy controls. According to the receiver operating characteristic curve analysis, the three genes had the power to discriminate the patients from the ERBB1 and ERBB2 expressions in the patients. in schizophrenia.

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  İncele
• MakaleAçık Erişim2023/r/bayburt/14498
  Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

  Dirican, Ebubekir; Kaya, Yasemin

  Purpose: Type 2 diabetes mellitus (T2DM) is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1-94 ATTG ins/del polymorphisms on the expression of apoptosis genes and telomere length (TL) in patients with T2DM compared with healthy individuals.Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized.Results: There was a significant difference between the NFKB1-94 ins/del genotype patients and the control group (OR:0.4792 (0.2345-1.011)). However, the distribution of other genotype/alleles (ins/ins and del/del) showed no difference between T2DM and control groups. The allelic frequency of NFKB1-94 ins/del was 0.455/0.235 for the T2DM group and 0.435/0.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals.Conclusion: NFKB1-94 ins/del polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.

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  İncele
• MakaleAçık Erişim2023/r/bayburt/14329
  LRIG1 levels in chronic rhinosinusitis with nasal polyps

  Uzunçakmak, Sevgi Karabulut; Sahin, Abdulkadir; Özçelik, Aysegul Tavaci; Halici, Zekai

  Purpose: Nasal polyps (NPs), usually occurring together with chronic rhinosinusitis (CRS), are benign masses of mucosal origin arising from inflammation. The transmembrane protein known as leucine-rich repeats and immunoglobulin-like domains 1 (Lrig1) is a member of the Lrig family. Lrig1 is frequently expressed in the respiratory tract and epithelial tissues and can inhibit several signaling pathways involved in cell proliferation. The aim of this study was to determine Lrig1 levels in NP tissues of patients with CRS. Material and Methods: This study included 36 patients with CRS and NPs and 15 patients who underwent rhinoplasty as the control group. The Lrig1 levels of all participants were measured by the ELISA method.Results: This study revealed that Lrig1 levels were significantly lower in NP tissues than in tissues of the control group. The mean level of Lrig1 of the NP tissues was 22.2 ng/ml, while the mean level of the control group was 28.5 ng/ml. According to the results of ROC analysis, Lrig1 levels have the power to distinguish polyp tissues from control tissues (AUC=0.794). Lrig1 levels were higher in tissues with scores of 4-8 than in tissues with scores of 16-20 based on the results of computed tomography scoring. According to endoscopic evaluations, Lrig1 levels of tissues with scores of 5-8 or 9-11 were relatively lower than those of tissues with scores of 2-4.Conclusion: Lrig1 levels were found to be decreased in NP tissues. Thus, Lrig1 may be used to confirm the presence of NPs. Lrig1 may also be helpful in NP grading. Increasing the Lrig1 levels in cases of NPs has the potential to become a targetable treatment modality.

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  AtıfWoS1·OpenAlex1·TR Dizin1

  İncele
• MakaleAçık Erişim2023/r/bayburt/14087
  Analysis of telomere length in patients with COVID-19 and investigation into its relationship with clinical- demographic data

  Savrun, Atakan; Dirican, Ebubekir

  Purpose: Novel coronavirus disease 2019 (COVID-19) is an infectious disease unknown before the 2019 outbreak in Wuhan. This study evaluated telomere length in COVID-19 (+) and (-) samples with clinical-demographic parameters.Materials and Methods: DNA was isolated from COVID-19 (+) (n=70) and (-) (n=70) patients. Telomere length was determined by real-time-PCR (RT-PCR). The 2- increment increment Ct method was used to analyze the telomere length of the samples.Results: There were significant differences in creatinine, LDH, ferritin, WBC, NEU and CRP in COVID-19 (+) patients compared to COVID-19 (-) patients. The NEU/LYM (or N/L) ratio was found higher in the patients with COVID-19 (+), than in COVID-19 (-). On the other hand, our COVID-19 (+) patients (mean +/- std:0.93 +/- 0.58) had significantly shorter telomere lengths than the COVID-19 (-) (mean +/- std:1.26 +/- 0.76). Moreover, COVID-19 (+) male patients (mean +/- std:1.06 +/- 0.50) had longer telomere length than female patients (mean +/- std:0.76 +/- 0.54). Telomere length was significantly shorter in patients with COVID-19 (+) with high blood urea nitrogen (BUN), high creatinine, high hematocrit, high NEU levels, normal platelets (PLT), and low WBC levels.Conclusion: Our findings suggest that telomere length and blood parameter levels influence the severity of COVID-19. Blood parameters differed in patients with COVID-19 (+) and COVID-19 (-). As a result, increasing the number of similar studies in the future can demonstrate the significance of our findings.

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  AtıfWoS2·OpenAlex2

  İncele
• MakaleAçık Erişim2022/r/bayburt/14522
  CYB mtDNA mutations and expression status of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia

  Dirican, Ebubekir; Uzunçakmak, Sevgi Karabulut; Özcan, Halil

  Purpose: This study aimed to screen for cytochrome b (CYB) mitochondrial DNA (mtDNA) mutations and analyze the mRNA expressions of genes in the PI3K/AKT/mTOR signaling pathway in patients with schizophrenia. Materials and Methods: In this study, whole blood was obtained from 44 schizophrenic patients and 41 healthy individuals for DNA (patients) and RNA (patients and control) isolation. Samples for CYB mtDNA mutations were amplified by PCR and identified by Sanger DNA sequencing. RT-PCR and 2- increment increment Ct method was used for mRNA expression of PIK3CA, AKT1, and mTOR genes. Results: In schizophrenia patients, m.15326 A > G (43/44), m.15452 C > A (5/44), m.15078 A > G (3/44), m.14872 C > T (3/44) and m.14798 T > C (3/44) was the most common CYB mtDNA mutations. In silico analysis showed that some of the mutations were associated with the harmful, disease-causing or benign character. The mRNA expression of the PIK3CA, AKT1, and mTOR genes in schizophrenia patients was significantly higher than in healthy individuals. There was a significant moderate positive correlation between the PIK3CA and AKT1 genes. In addition, by ROC analysis, PIK3CA, AKT1 and mTOR genes were found to have good diagnostic power in the patient group. ROC analyzes showed that PIK3CA in particular has significant diagnostic value for schizophrenia patients with 80% sensitivity and 63.4% specificity. Conclusion: Both of CYB mtDNA mutations frequency and PIK3CA, AKT1 and mTOR mRNA expression were higher in schizophrenic patients compared to healthy individuals. We believe that studying these mechanisms in

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  AtıfWoS1·OpenAlex1·TR Dizin1

  İncele
• MakaleAçık Erişim2022/r/bayburt/14521
  Impact of gossypin on gene expression of HSP60 and HSP70 in different cancer cell lines

  Dirican, Ebubekir; Çinar, Trfan

  Purpose: The aim of this study is to evaluate the impact of gossypin on the expression level of heat shock proteins (HSPs) genes in different cancer cells. Materials and Methods: Cells were grown under standard culture conditions. Cancer cells were treated with different concentrations (5-100 mu g/ml) of gossypin and cisplatin (50 mu M) as positive control. Cell viability and effective dose range (5-100 mu g/ml) of gossypin were determined by MTT at 24, 48 and 72 hours. After RNA isolation and cDNA synthesis, HSP60 and HSP70 gene expression levels were analyzed using RT-PCR. For gene expression analysis, the 2-(Delta Delta ct) method was used. Results: According to the Mil results, 25-50-100 mu g/ml of gossypin doses were found effective on HSP60 and HSP70 gene expression levels in the cancer cell lines. Gossypin affected with dose-dependently the expression of HSP60 and HSP70 in the three cell lines. In the three cell lines, 50 mu g/ml and 100 mu g/ml of gossypin doses significantly reduced the expression of HSP60 and HSP70 compared to control group. Conclusion: Our results strongly supported the anticarcinogenic effect of gossypin at various doses in different cell lines. However, we believe that further in vivo research and human studies are needed. Our findings suggest that gossypin could be suitable candidate agent for further investigation to develop new strategies for the prevention and/or treatment of different cancer types.

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  AtıfWoS1·OpenAlex2

  İncele
• MakaleAçık Erişim2022/r/bayburt/14508
  Investigation of the effect of gossypin on MMP-2 and MMP- 9 genes in prostate cancer cells

  Dirican, Ebubekir; Çinar, Irfan

  Purpose: The aim of this study is to explore the effects of gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials and Methods: PC3 cells were grown in culture and treated with three different concentrations of gossypin (25-50-100 mu g/ml) and cisplatin (50 mu M) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis was performed and RT-PCR was used to evaluate mRNA expression levels of MMP-2 and MMP-9 genes. Results: Gossypin decreased MMP-2 and MMP-9 mRNA expression in prostate cancer cells in a concentrationdependent manner. Three concentrations (25-50-100 mu g/ml) of gossypin in PC3 cells reduced the mRNA expression of the MMP-2 gene. While the fold change value of MMP-2 gene expression was 0.3482 +/- 0.040 in the 100 mu g/ml gossypin group, it was 1.007 +/- 0.1425 in the control group. In addition, 50 mu g/ml and 100 mu g/ml concentrations of gossypin decreased the mRNA expression of the MMP-9 gene. The expression level of the MMP-9 gene in prostate cancer cells was 0.4740 +/- 0.038 in the 100 mu g/ml gossypin administered group, while it was 1.009 +/- 0.1687 in the control group. There was a positive correlation between the expressions of the MMP-2 and MMP-9 genes. Conclusion: According to the results obtained, it is seen that gossypin reduces the expression of MMP-2 and MMP9 genes in prostate cancer cells and the effects of gossypin on other genetic and epigenetic mechanisms in cancer need to be investigated to reveal the anti-cancer.

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  AtıfWoS2·OpenAlex2·TR Dizin1

  İncele
• MakaleAçık Erişim2021/r/bayburt/14504
  Expression level of the angiotensin 2 (ACE2) gene in patients with COVID-19

  Dirican, Ebubekir; Aydin, Ismail Erkan; Savrun, Seyda

  Purpose: Coronavirus disease-19 (COVID-19) is caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV2). The angiotensin converting enzyme 2 (ACE2) can play a vital role in regulating the pathological changes of various diseases, including COVID-19. The aim of this study was to analyze the expression of the ACE2 gene in Turkish COVID-19 patients. Materials and Methods: Eighty COVID-19 PCR test positive patients and 80 PCR test negative healthy volunteers were included in the study. RNA isolation was carried out from all samples. The RT-PCR device was used to analyze the expression of the ACE2 gene. Results: A significant difference was found in the age, Lactate dehydrogenase (LDH), ferritin and C-reactive protein (CRP) levels of the COVID-19 patients compared to the healthy volunteers. Based on the gene expression results, it was found that ACE2 gene expression was significantly higher in COVID-19 patients compared to healthy volunteers. In patients with COVID-19, a significant difference was found between the expression of the ACE2 gene and the levels of Blood urea nitrogen (BUN), Hemoglobin (HGB), Hematocrit (HCT) and CRP. Conclusion: ACE2 gene expression was high in the COVID-19 patients and showed differences based on clinical data. Thus, the expression of ACE2 can have paradoxical effects that support the pathogenicity of SARSCoV2 but, conversely, limit viral infection. The availability of ACE2 receptors may increase the susceptibility and / or disease course of COVID-19.

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  AtıfWoS1·OpenAlex1

  İncele
• MakaleAçık Erişim2020/r/bayburt/14469
  Attitudes of women toward for early diagnosis of cervical cancer: health responsibility and associated factors

  Önal, Betul; Yilmaz, Figen Alp

  Purpose: This study was conducted to determine the attitudes of women toward early diagnosis of cervical cancer, and the relationship between these attitudes, and health responsibility and some factors. Materials and Methods: This study was performed with 662 women aged 21 to 65. The Descriptive Characteristics Form, Attitude Scale for Early Diagnosis of Cervical Cancer and Healthy Lifestyle Behaviors Scale II-Health Responsibility Subscale were used for data collection. Results: In the present study, it was detected that perceived susceptibility of women were at moderate levels while their perceived severity, barriers and benefits were at low levels. A negative correlation was detected between Health Responsibility and Perceived Susceptibility Among the significant variables that impact attitudes toward early diagnosis of cervical cancer are women's individual and some obstetric characteristics, their health responsibility. Conclusion: The participating women's perceived susceptibility of attitudes toward early diagnosis of cervical women was at a moderate level while their perceived severity, barriers and benefits were at a low level. Age, educational attainment, income level, presenting to the clinic for regular gynecological examinations and history of gynecological cancer in the family were some factors that had an impact on the health responsibility and attitudes of the participating women toward early diagnosis of cervical cancer. In the light of these findings, evidence-based, individualized nursing services such as physical and psychosocial care, training and consultancy can be planned to improve women's attitudes toward early diagnosis of cervical cancer.

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  AtıfWoS5·OpenAlex6·TR Dizin2

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